##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n01_30521-1.341000000356df.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3070365 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.69357975354722 33.0 33.0 33.0 33.0 33.0 2 32.7126286288438 33.0 33.0 33.0 33.0 33.0 3 32.74322629394225 33.0 33.0 33.0 33.0 33.0 4 36.680702457199715 37.0 37.0 37.0 37.0 37.0 5 36.641179468890506 37.0 37.0 37.0 37.0 37.0 6 36.5203169655725 37.0 37.0 37.0 37.0 37.0 7 36.45828590411889 37.0 37.0 37.0 37.0 37.0 8 36.37915850395637 37.0 37.0 37.0 37.0 37.0 9 36.39387369254144 37.0 37.0 37.0 37.0 37.0 10-11 36.330385801036684 37.0 37.0 37.0 37.0 37.0 12-13 36.32864643128748 37.0 37.0 37.0 37.0 37.0 14-15 38.17226844365409 40.0 37.0 40.0 37.0 40.0 16-17 38.15556114663892 40.0 37.0 40.0 37.0 40.0 18-19 38.128804067268874 40.0 37.0 40.0 37.0 40.0 20-21 38.05255824633228 40.0 37.0 40.0 37.0 40.0 22-23 37.93059929356933 40.0 37.0 40.0 33.0 40.0 24-25 37.932727379318095 40.0 37.0 40.0 33.0 40.0 26-27 37.841678920910056 40.0 37.0 40.0 33.0 40.0 28-29 37.69543930444752 40.0 37.0 40.0 33.0 40.0 30-31 37.60551253678309 40.0 37.0 40.0 33.0 40.0 32-33 37.48959260543942 40.0 37.0 40.0 33.0 40.0 34-35 37.37001480280031 40.0 37.0 40.0 33.0 40.0 36-37 37.295112307494385 40.0 37.0 40.0 33.0 40.0 38-39 37.21092492260692 40.0 37.0 40.0 33.0 40.0 40-41 37.11253857440402 40.0 37.0 40.0 33.0 40.0 42-43 36.898902084931265 37.0 37.0 40.0 33.0 40.0 44-45 36.74881048344415 37.0 37.0 40.0 33.0 40.0 46-47 36.68785144437225 37.0 37.0 40.0 33.0 40.0 48-49 36.45216431922589 37.0 37.0 40.0 33.0 40.0 50-51 36.286429137903795 37.0 37.0 40.0 33.0 40.0 52-53 36.12955430380427 37.0 37.0 40.0 33.0 40.0 54-55 35.88773680002215 37.0 37.0 40.0 33.0 40.0 56-57 35.66688260190564 37.0 35.0 40.0 27.0 40.0 58-59 35.40955456435961 37.0 33.0 40.0 27.0 40.0 60-61 35.11861944752497 37.0 33.0 38.5 27.0 40.0 62-63 34.82300622238724 37.0 33.0 37.0 27.0 40.0 64-65 34.508742934471954 37.0 33.0 37.0 27.0 40.0 66-67 34.12339738109313 37.0 33.0 37.0 27.0 40.0 68-69 33.847378731844586 37.0 33.0 37.0 27.0 38.5 70-71 33.535006587164716 37.0 33.0 37.0 27.0 37.0 72-73 33.0529060877127 37.0 33.0 37.0 27.0 37.0 74-75 32.5647624956642 35.0 33.0 37.0 22.0 37.0 76-77 29.754651319957073 33.0 27.0 33.0 22.0 37.0 78-79 32.3030711332366 33.0 33.0 37.0 24.5 37.0 80-81 32.78576130199504 37.0 33.0 37.0 27.0 37.0 82-83 32.74140240655427 37.0 33.0 37.0 27.0 37.0 84-85 32.638498028735995 37.0 33.0 37.0 24.5 37.0 86-87 32.48614285272272 33.0 33.0 37.0 22.0 37.0 88-89 32.35508677307095 33.0 33.0 37.0 22.0 37.0 90-91 32.21746942138801 33.0 33.0 37.0 22.0 37.0 92-93 32.06328905521005 33.0 33.0 37.0 22.0 37.0 94-95 31.978355342117304 33.0 33.0 37.0 22.0 37.0 96-97 31.832130707586884 33.0 33.0 37.0 22.0 37.0 98-99 31.521297630737713 33.0 33.0 37.0 18.5 37.0 100-101 29.771630408762476 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.08717080501709518 2306 2 0.1333815127949194 2306 3 0.12446786300132118 2306 4 0.12669679818292678 2306 5 0.17150338163610002 2306 6 0.189552289670452 2306 7 0.20871260563393434 2306 8 0.2312467822653943 2306 9 0.21704513502827183 2306 10-11 0.26229745824286965 2306 12-13 0.24107342793438136 2306 14-15 0.5263640420165956 2306 16-17 0.5209100635296764 2306 18-19 0.520502220450183 2306 20-21 0.59321666115531 2306 22-23 0.565835410573591 2306 24-25 0.5490720102143669 2306 26-27 0.556578920739554 2306 28-29 0.5481761175646227 2306 30-31 0.4818572564878423 2306 32-33 0.5115252070255849 2306 34-35 0.6587568986797194 2306 36-37 0.6839018614631769 2306 38-39 0.7530394325123666 2306 40-41 0.6912281924105912 2306 42-43 0.7632253944263354 2306 44-45 0.6521215667664322 2306 46-47 0.6155599801340301 2306 48-49 0.6664974805853063 2306 50-51 0.5846614211726404 2306 52-53 0.6331686975490385 2306 54-55 0.6419057003281594 2306 56-57 0.5704995663241519 2306 58-59 0.6802823101013828 2306 60-61 0.6204741905510005 2306 62-63 0.7020907854773313 2306 64-65 0.6109687650699982 2306 66-67 0.6371767959213273 2306 68-69 0.6230140069574261 2306 70-71 0.6984327120304883 2306 72-73 0.43220846277234415 2306 74-75 0.27437242290312014 2306 76-77 -0.006814554031102205 2306 78-79 0.33942054401625654 2306 80-81 0.44550355383418605 2306 82-83 0.44736019143989125 2306 84-85 0.47610413575569765 2306 86-87 0.3955308818779173 2306 88-89 0.4565208276952788 2306 90-91 0.6369283456396175 2306 92-93 0.5010374590676108 2306 94-95 0.3887355589841164 2306 96-97 0.5661550850537793 2306 98-99 0.4374091596272791 2306 100-101 0.3622737127228213 2307 1 0.11761428493558412 2307 2 0.08686471357371772 2307 3 0.12445281840233946 2307 4 0.17357668595755626 2307 5 0.21434581276256637 2307 6 0.2022572700381673 2307 7 0.17193094624769145 2307 8 0.27998265983612214 2307 9 0.20011042069860707 2307 10-11 0.25983684069144175 2307 12-13 0.24970361536256291 2307 14-15 0.5788087801772761 2307 16-17 0.5608294391568052 2307 18-19 0.514202661568838 2307 20-21 0.5621321345437025 2307 22-23 0.5062501754898037 2307 24-25 0.5277040928950711 2307 26-27 0.544762489065242 2307 28-29 0.6080268347791886 2307 30-31 0.6280148016936451 2307 32-33 0.6690580285612384 2307 34-35 0.5365236600600909 2307 36-37 0.5768162406322119 2307 38-39 0.5655087814353266 2307 40-41 0.6826990056133582 2307 42-43 0.617796319711374 2307 44-45 0.5401372777310698 2307 46-47 0.5694661726560923 2307 48-49 0.6132546447958163 2307 50-51 0.5381096707794129 2307 52-53 0.5859677360156894 2307 54-55 0.6919199101353684 2307 56-57 0.6722937081407778 2307 58-59 0.6317781560490872 2307 60-61 0.6615975927733047 2307 62-63 0.4226419461956681 2307 64-65 0.5243905838849656 2307 66-67 0.4585515383513723 2307 68-69 0.6971723212724896 2307 70-71 0.4916014916988516 2307 72-73 0.41076119436696956 2307 74-75 0.5165971631590338 2307 76-77 0.20220996701399585 2307 78-79 0.4481103410811329 2307 80-81 0.65237605507766 2307 82-83 0.6481165127859256 2307 84-85 0.5715504658918036 2307 86-87 0.6647086633536432 2307 88-89 0.6926154442790846 2307 90-91 0.7605222031133252 2307 92-93 0.7283597901511669 2307 94-95 0.7247227351147245 2307 96-97 0.7566745658830172 2307 98-99 0.67278824040595 2307 100-101 0.3992352651493043 2201 1 0.08829079041643695 2201 2 0.0650682394558757 2201 3 0.11535252944091212 2201 4 0.16632620734066705 2201 5 0.16605165419169765 2201 6 0.1367221557143239 2201 7 0.22155202770520077 2201 8 0.3008033048318737 2201 9 0.1855216047239452 2201 10-11 0.21918562488000504 2201 12-13 0.1599917374408406 2201 14-15 0.3954038291751516 2201 16-17 0.3362302541447235 2201 18-19 0.41020691427308975 2201 20-21 0.38588451483317954 2201 22-23 0.4534898926904347 2201 24-25 0.43317226738833625 2201 26-27 0.475272366605509 2201 28-29 0.41739690978496213 2201 30-31 0.5050056551722264 2201 32-33 0.5441899446221896 2201 34-35 0.508458678029335 2201 36-37 0.6476668940794568 2201 38-39 0.5984511177817922 2201 40-41 0.5872262385816498 2201 42-43 0.5606417350716697 2201 44-45 0.5500269305645062 2201 46-47 0.5979285662147973 2201 48-49 0.5640635593701688 2201 50-51 0.5308990271450398 2201 52-53 0.5153133572895854 2201 54-55 0.5239799741238258 2201 56-57 0.47371128760005377 2201 58-59 0.5503151049999175 2201 60-61 0.4938922310699567 2201 62-63 0.4173988265586672 2201 64-65 0.3714746631645909 2201 66-67 0.4152034003937004 2201 68-69 0.6215003554442546 2201 70-71 0.6015829782414599 2201 72-73 0.5361551512551088 2201 74-75 0.47716564845045895 2201 76-77 0.24043022529481206 2201 78-79 0.6527582561334953 2201 80-81 0.5515448431490739 2201 82-83 0.545343793283223 2201 84-85 0.5593652262285218 2201 86-87 0.6217916303245516 2201 88-89 0.7464764350322497 2201 90-91 0.7966765867868162 2201 92-93 0.8913563498417076 2201 94-95 0.9358234908363698 2201 96-97 0.9280608676305349 2201 98-99 0.8572442549082737 2201 100-101 0.5345300491379277 1101 1 0.10137352848843761 1101 2 0.07769837445612637 1101 3 0.12582466786496127 1101 4 0.13313066856333222 1101 5 0.1517948123264432 1101 6 0.1127348124912615 1101 7 0.1154855356361324 1101 8 0.23252312835075628 1101 9 0.24381725211362237 1101 10-11 0.22083819920324999 1101 12-13 0.20348810081698332 1101 14-15 0.3752712019150053 1101 16-17 0.38098916633600766 1101 18-19 0.40930743924309354 1101 20-21 0.4414442525526425 1101 22-23 0.40371563660445275 1101 24-25 0.4433922368182692 1101 26-27 0.4831518731969098 1101 28-29 0.4392879046257221 1101 30-31 0.4663163019702026 1101 32-33 0.3923936587554877 1101 34-35 0.4626114738523768 1101 36-37 0.5129411170179168 1101 38-39 0.4894357354731511 1101 40-41 0.4848228574105491 1101 42-43 0.535342896226247 1101 44-45 0.5316158058845915 1101 46-47 0.4850435980271257 1101 48-49 0.4724315736849434 1101 50-51 0.4505446336734309 1101 52-53 0.45938589893619053 1101 54-55 0.5274268516582978 1101 56-57 0.48738231672836463 1101 58-59 0.5211246851912108 1101 60-61 0.48416219439702246 1101 62-63 0.586816427706637 1101 64-65 0.43373964878724536 1101 66-67 0.45817575412174705 1101 68-69 0.5776420025476909 1101 70-71 0.6916991618527035 1101 72-73 0.6484174417054689 1101 74-75 0.5934553627436046 1101 76-77 0.3028487118223602 1101 78-79 0.6996832194388816 1101 80-81 0.5744900530844035 1101 82-83 0.5361992595573284 1101 84-85 0.595107083245594 1101 86-87 0.7250607194244765 1101 88-89 0.732451625816374 1101 90-91 0.7569847534067051 1101 92-93 0.82851701370911 1101 94-95 0.822588614935114 1101 96-97 0.8449759858122974 1101 98-99 0.7406473839103711 1101 100-101 0.8248324784485135 2304 1 0.08182176053679058 2304 2 0.06885564753964246 2304 3 0.09024001478564259 2304 4 0.12124800687877979 2304 5 0.17270228035368973 2304 6 0.10779491849314837 2304 7 0.15486416980294138 2304 8 0.16099757983648288 2304 9 0.16035956380984828 2304 10-11 0.1533451162423276 2304 12-13 0.13859954530056484 2304 14-15 0.26751877451917494 2304 16-17 0.3273776604995646 2304 18-19 0.2897168804571635 2304 20-21 0.31833580050484045 2304 22-23 0.38261421440580534 2304 24-25 0.3201818598742747 2304 26-27 0.3849210123174629 2304 28-29 0.3330376324961435 2304 30-31 0.36388178129429605 2304 32-33 0.36351229933465845 2304 34-35 0.36136343813314653 2304 36-37 0.28267397483367773 2304 38-39 0.4005691007363481 2304 40-41 0.25353646626436444 2304 42-43 0.3478733252607924 2304 44-45 0.37411611264884215 2304 46-47 0.3225337518842437 2304 48-49 0.3505749176923487 2304 50-51 0.36860988143999407 2304 52-53 0.4275210097869433 2304 54-55 0.26151271398250486 2304 56-57 0.3966889503568467 2304 58-59 0.33628932853323334 2304 60-61 0.3550728290229941 2304 62-63 0.47594786926111254 2304 64-65 0.48040541825093896 2304 66-67 0.348976586937205 2304 68-69 0.22959816890494267 2304 70-71 0.38227199956055813 2304 72-73 0.3750489327380606 2304 74-75 0.400252268976665 2304 76-77 0.1949062885035282 2304 78-79 0.3942034203595739 2304 80-81 0.32741420908689634 2304 82-83 0.3532737577503866 2304 84-85 0.27881532867283454 2304 86-87 0.3509508305325113 2304 88-89 0.531496302566012 2304 90-91 0.4063918077970001 2304 92-93 0.3071761174825127 2304 94-95 0.3639275716200032 2304 96-97 0.31830827680688145 2304 98-99 0.4815889324563116 2304 100-101 0.3583271763747895 2202 1 0.07714337139932326 2202 2 0.05554216120169286 2202 3 0.042664072852019785 2202 4 0.07929742253636363 2202 5 0.06362984205892985 2202 6 0.08207185234767422 2202 7 0.07785658486526614 2202 8 0.058898284817935576 2202 9 0.060346934062827984 2202 10-11 0.09404497489943964 2202 12-13 0.08037536125180367 2202 14-15 0.07456486049230193 2202 16-17 0.13533461117975065 2202 18-19 0.09094533997725307 2202 20-21 0.13676027763726495 2202 22-23 0.1990321765843106 2202 24-25 0.06920600842320113 2202 26-27 0.18915671700614922 2202 28-29 0.022759604763741947 2202 30-31 0.14138305542834217 2202 32-33 0.13378488130919663 2202 34-35 0.17324420964526865 2202 36-37 0.1186500586615864 2202 38-39 0.13245906754453074 2202 40-41 0.08140345165928409 2202 42-43 0.20739141507588243 2202 44-45 0.18825317139869924 2202 46-47 0.0752882708261069 2202 48-49 0.12262163777855761 2202 50-51 0.14730290743022323 2202 52-53 0.16568843349446638 2202 54-55 0.16045274736599424 2202 56-57 0.23747628510999874 2202 58-59 0.15340330530447943 2202 60-61 0.240280572607098 2202 62-63 0.2546193642896952 2202 64-65 0.14904004544628435 2202 66-67 0.25723998621916877 2202 68-69 0.2863372417449739 2202 70-71 0.1510143078214341 2202 72-73 0.1575392851605102 2202 74-75 0.08300549606082797 2202 76-77 0.09748525133609931 2202 78-79 0.2055587838927906 2202 80-81 0.25959712825992654 2202 82-83 0.4191968360267211 2202 84-85 0.3422494146533097 2202 86-87 0.35128823645599283 2202 88-89 0.23320865732064533 2202 90-91 0.2949067202198066 2202 92-93 0.28789435360311444 2202 94-95 0.3290784629624284 2202 96-97 0.34983761313598905 2202 98-99 0.29859742064408223 2202 100-101 0.11007394128215608 1102 1 0.08132323023979637 1102 2 0.039566647000519595 1102 3 0.08281810885920748 1102 4 0.09988233295806026 1102 5 0.12775437658122257 1102 6 0.0657194389703406 1102 7 0.13960288438146762 1102 8 0.13053960303755474 1102 9 0.05062307986221981 1102 10-11 0.053697430254835865 1102 12-13 0.04702014552249523 1102 14-15 0.1475422495530836 1102 16-17 0.12036518530355522 1102 18-19 0.1355677813178744 1102 20-21 0.13003077838075683 1102 22-23 0.14190689705677784 1102 24-25 0.14162563729374966 1102 26-27 0.1280917630284435 1102 28-29 0.08408395547390768 1102 30-31 0.05361547538242917 1102 32-33 0.010365036224513346 1102 34-35 0.027777991146130887 1102 36-37 0.14221812184670313 1102 38-39 0.05633091459742445 1102 40-41 0.14012233173029642 1102 42-43 -0.07690138101852995 1102 44-45 0.07523702038366764 1102 46-47 0.11059447279901491 1102 48-49 0.06309397230699432 1102 50-51 -0.00893583392647912 1102 52-53 -0.001677445988136128 1102 54-55 0.012146715834866484 1102 56-57 -0.01875191656056785 1102 58-59 -0.06885639135733612 1102 60-61 -0.15571946593804142 1102 62-63 0.11930750099966048 1102 64-65 0.04751492274927216 1102 66-67 -0.023750274508572033 1102 68-69 0.053513045516503155 1102 70-71 0.05772670267300839 1102 72-73 -0.025181230669794274 1102 74-75 -0.16724423427377388 1102 76-77 -0.16477785416134338 1102 78-79 0.0984790056959639 1102 80-81 -0.10159875873296187 1102 82-83 0.05420371091341991 1102 84-85 0.11081633654099221 1102 86-87 0.06277480799245438 1102 88-89 -0.028698748826464282 1102 90-91 0.18880098970212345 1102 92-93 0.2130637263592874 1102 94-95 0.1533497942331934 1102 96-97 0.009278431593820358 1102 98-99 -0.12636543449064064 1102 100-101 -0.2037414793025114 2305 1 0.03850529277572434 2305 2 0.0355926155896924 2305 3 0.05033046021926424 2305 4 0.048361019732446664 2305 5 0.02197003060802416 2305 6 -0.046817426835353615 2305 7 0.0036587376691272766 2305 8 0.1118237900849337 2305 9 -0.026028668820131884 2305 10-11 0.0758217866606472 2305 12-13 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-0.40210265748213203 1213 68-69 0.04795883741560658 1213 70-71 0.22314705799680468 1213 72-73 0.3307451275644979 1213 74-75 0.3758971373003206 1213 76-77 -2.029653248291833 1213 78-79 -1.8602146033265896 1213 80-81 -1.2956178285850086 1213 82-83 -0.7342217377889853 1213 84-85 -0.44086478157688447 1213 86-87 -0.09119302489622072 1213 88-89 -0.30065105903747735 1213 90-91 -1.4721368892695068 1213 92-93 -0.6627730429834209 1213 94-95 -0.2135114111997396 1213 96-97 -0.3208710348141395 1213 98-99 -2.1461667017717474 1213 100-101 -0.6955382595596085 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 6.0 9 9.0 10 19.0 11 31.0 12 35.0 13 108.0 14 232.0 15 662.0 16 1356.0 17 2133.0 18 3271.0 19 4480.0 20 6130.0 21 8057.0 22 10732.0 23 13538.0 24 17409.0 25 22208.0 26 28643.0 27 38143.0 28 53992.0 29 75104.0 30 79523.0 31 83180.0 32 110504.0 33 156148.0 34 240976.0 35 408140.0 36 710937.0 37 834908.0 38 159719.0 39 32.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.984835840139475 25.525183010659937 18.256630568049168 16.233350581151416 2 32.802147889193364 23.82377302676686 28.121310269733634 15.252768814306144 3 38.66633618961479 18.92578238867814 24.213198085274154 18.194683336432913 4 33.217158220602435 18.605768369558668 27.4713592683606 20.705714141478293 5 36.92453605104603 17.046425348179525 26.928011250918875 19.10102734985557 6 33.00447992339673 17.83077907675472 29.695329382662973 19.469411617185578 7 34.86305374116758 20.324619385643075 23.272444807050626 21.539882066138716 8 31.685972188974276 25.895846259320958 21.716375740343572 20.70180581136119 9 31.93242497227528 22.143263097384185 24.384039031190103 21.540272899150427 10-11 24.905984105764848 28.70008502264475 28.600389824185022 17.793541047405377 12-13 23.74444080752614 27.49419043012801 28.18515062541424 20.576218136931605 14-15 24.00766684091305 27.581134490524743 27.818386413341734 20.592812255220473 16-17 25.015527469861077 26.085025721697587 28.18541118075538 20.71403562768596 18-19 23.187947229066776 26.027544938068424 29.32716946147599 21.457338371388804 20-21 23.919126879051838 26.40593219372941 29.07450742826993 20.600433498948824 22-23 22.533216734818172 29.063205840347972 27.6380006937286 20.76557673110526 24-25 22.645564940976072 25.59736383133601 29.58599384763701 22.171077380050903 26-27 22.315131914283807 25.73156611673205 28.652082081446345 23.3012198875378 28-29 23.400035500665233 27.988512766397484 27.700631683855175 20.910820049082112 30-31 21.52071496385609 27.732533428436035 28.901677813549853 21.845073794158022 32-33 21.279375579124956 28.894463687542032 27.78817827847829 22.037982454854717 34-35 21.59015296226996 28.746679954989062 29.280085592429568 20.383081490311415 36-37 21.083910219143327 29.152283197600287 29.726107482335163 20.037699100921223 38-39 21.243645493745767 27.622619558843336 29.16098940173312 21.97274554567778 40-41 22.767251700449847 27.285755044027354 27.625258764107446 22.321734491415352 42-43 21.895492530760578 28.28925983755471 28.607027397090732 21.20822023459398 44-45 21.397327858163216 27.02454381362882 28.80786074273062 22.770267585477338 46-47 22.653279916617812 27.08422904045339 28.067943456452348 22.19454758647645 48-49 21.24221069650672 28.762968058481498 29.001353641869876 20.99346760314191 50-51 22.70487727777815 27.470091196627365 27.76682183409826 22.058209691496224 52-53 21.480600983703845 26.90584069678099 27.215198325338825 24.39835999417634 54-55 23.04270465531659 26.82016731606618 28.894385520166242 21.24274250845099 56-57 22.126292401931526 27.040542695212284 28.060164615072164 22.773000287784033 58-59 21.929531521321486 27.56129926308463 28.063668262830703 22.445500952763176 60-61 22.959246325583152 27.30455796088635 28.220218437375717 21.515977276154782 62-63 21.694458151544612 27.082130229937285 28.56822960179924 22.65518201671886 64-65 23.224918663753286 27.283213006502994 28.477898169755296 21.01397015998843 66-67 22.241843986072364 29.62899554071389 27.2162407607257 20.912919712488037 68-69 22.54475964962465 29.345564629369836 27.068567932301946 21.041107788703567 70-71 22.04002188141744 29.61861301918273 27.116080658892617 21.22528444050721 72-73 21.968139330239815 29.83584558290521 26.822937962036562 21.37307712481841 74-75 22.36583175806482 29.06507243628715 26.81375797489866 21.755337830749372 76-77 22.132186506045688 28.470541281302562 26.69797635483227 22.69929585781948 78-79 21.759775935767212 28.465578269052983 27.16609432301551 22.608551472164294 80-81 21.30799519849369 28.48356334172723 27.66344443751422 22.54499702226486 82-83 20.776976027280146 28.532975721127617 27.66926733466542 23.020780916926814 84-85 20.781751191459534 29.150239855331144 27.41251012968604 22.65549882352328 86-87 20.652921790643337 29.148515290159228 27.632758613949377 22.565804305248054 88-89 20.97705352765158 29.031514545362736 27.581343754021376 22.41008817296431 90-91 21.470743266840067 28.91904896559273 26.81734723662881 22.792860530938395 92-93 21.447017900303507 28.845841510400316 26.775177641977965 22.931962947318212 94-95 21.718691764166213 29.168219622234325 26.61981953570321 22.49326907789625 96-97 21.736567241860428 29.829136029965742 26.444827548571425 21.989469179602402 98-99 21.88709768071531 29.427603503567347 26.624521550295157 22.06077726542219 100-101 21.981961437155793 29.486974411433653 26.35544145937414 22.175622692036416 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 24.0 1 15.0 2 20.5 3 44.5 4 40.5 5 22.5 6 20.0 7 29.5 8 33.0 9 28.5 10 29.0 11 29.5 12 26.5 13 33.0 14 38.0 15 40.5 16 45.0 17 66.0 18 103.0 19 155.0 20 222.5 21 296.5 22 424.0 23 611.0 24 893.5 25 1292.5 26 1799.5 27 2479.5 28 3339.0 29 4361.5 30 5826.5 31 8132.0 32 12829.5 33 21348.0 34 35369.0 35 55409.0 36 84646.5 37 119644.0 38 142825.5 39 147867.5 40 150589.5 41 155265.5 42 163577.0 43 177124.5 44 175746.5 45 165227.0 46 161851.0 47 157566.0 48 148172.0 49 132837.5 50 118540.5 51 114352.5 52 110298.0 53 99861.0 54 86176.0 55 71404.5 56 57839.5 57 44300.0 58 31795.0 59 23072.0 60 17343.5 61 13819.0 62 10966.5 63 8384.0 64 6335.5 65 4551.0 66 3252.0 67 2448.0 68 1925.5 69 1403.5 70 995.0 71 721.0 72 526.0 73 400.0 74 297.5 75 228.0 76 186.5 77 145.0 78 93.5 79 61.5 80 56.5 81 46.0 82 28.0 83 22.5 84 23.0 85 18.0 86 12.0 87 8.5 88 6.5 89 6.0 90 4.5 91 3.0 92 1.0 93 0.0 94 0.0 95 0.5 96 1.0 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1707614566997735 2 0.006123050516795235 3 0.0018238873879815592 4 0.0 5 0.001237637870416058 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.1170121467643097E-4 12-13 0.0 14-15 0.0 16-17 0.0 18-19 1.628470882126392E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 1.465623793913753E-4 40-41 3.256941764252784E-5 42-43 0.0012702072880585859 44-45 6.18818935208029E-4 46-47 0.005373953911017094 48-49 0.004201454875886091 50-51 0.007832944943027947 52-53 0.004299163128813675 54-55 0.019737067091371872 56-57 0.01154585855427612 58-59 0.01985106005312072 60-61 0.009314853445762963 62-63 0.005862495175655012 64-65 0.012799781133513441 66-67 0.030208134863444574 68-69 0.03162490453089453 70-71 0.03563094290092546 72-73 0.030550113748691113 74-75 0.034100180271726656 76-77 0.03346507662769736 78-79 0.017294360768182287 80-81 0.0025566992849384356 82-83 0.0 84-85 0.00589506459329754 86-87 0.026348658872805027 88-89 0.02703261664329811 90-91 0.025436715178814247 92-93 0.034507297992258254 94-95 0.03090837734275892 96-97 0.03235771642785141 98-99 0.03932757180335237 100-101 0.02703261664329811 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3070365.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.11260655493564 #Duplication Level Percentage of deduplicated Percentage of total 1 85.95726150282914 41.35627903225334 2 6.595183410461798 6.346229291703742 3 2.324079487554744 3.354525659613535 4 1.2349551074146345 2.376676367841944 5 0.6659298534773196 1.6019810516770108 6 0.4922244878189705 1.4209321871483305 7 0.3168835673062417 1.067226607827077 8 0.31051307534167744 1.195167473926177 9 0.19933186299064726 0.8631337948138214 >10 1.5411947085645896 14.470201565229193 >50 0.20544584683960337 6.979172527887778 >100 0.149079557340942 13.561985048664388 >500 0.006236606254750717 2.0442662302820347 >1k 0.0016130658840377884 1.1237302989070554 >5k 0.0 0.0 >10k+ 6.78599210762836E-5 2.2384928622244966 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT 68562 2.2330244124069942 TruSeq Adapter, Index 27 (97% over 39bp) GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 3769 0.12275413509468744 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 3645 0.11871552730701398 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.001465623793913753 0.0 0.0 2 0.0015307626291988087 0.0 0.0 3 0.0016936097174114477 0.0 0.0 4 0.0017587485526965034 0.0 0.0 5 0.0021170121467643096 0.0 0.0 6 0.002279859234976949 0.0 0.0 7 0.0026055534114022274 0.0 0.0 8 0.0030615252583976174 0.0 0.0 9 0.003452358270107951 0.0 0.0 10-11 0.005373953911017094 0.0 0.0 12-13 0.007295549551926237 0.0 0.0 14-15 0.009086867522265268 0.0 0.0 16-17 0.010764192530855452 0.0 0.0 18-19 0.012946343512904818 0.0 0.0 20-21 0.015942729936017377 0.0 0.0 22-23 0.0191833869914489 0.0 0.0 24-25 0.022603175843914323 0.0 0.0 26-27 0.025892687025809637 0.0 0.0 28-29 0.03050125962222732 0.0 0.0 30-31 0.06378720445289078 0.0 0.0 32-33 0.07070820570192794 0.0 0.0 34-35 0.07720580452161224 0.0 0.0 36-37 0.08432222227650459 0.0 0.0 38-39 0.09345794392523366 0.0 0.0 40-41 0.10342418572384716 0.0 0.0 42-43 0.11513289136633592 0.0 0.0 44-45 0.1284537831821298 0.0 0.0 46-47 0.14351713884179895 0.0 0.0 48-49 0.15972042411895654 0.0 0.0 50-51 0.17808957566934225 0.0 0.0 52-53 0.19763122625485896 0.0 0.0 54-55 0.22121148462804913 0.0 0.0 56-57 0.24729958815971392 0.0 0.0 58-59 0.2744624824735821 0.0 0.0 60-61 0.304377492578244 0.0 0.0 62-63 0.3368492019678442 0.0 0.0 64-65 0.3716659094277065 0.0 0.0 66-67 0.4121496955573686 0.0 0.0 68-69 0.45725833899226964 0.0 0.0 70-71 0.5074803809970476 0.0 0.0 72-73 0.5610733577278271 0.0 0.0 74-75 0.6235415007661955 0.0 0.0 76-77 0.6934354710270603 0.0 0.0 78-79 0.7709344003074553 0.0 0.0 80-81 0.8584972796393914 0.0 0.0 82-83 0.959788168507653 0.0 0.0 84-85 1.0743836644828872 0.0 0.0 86-87 1.2047101891794623 0.0 0.0 88-89 1.350067500118064 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAAGAGC 13620 0.0 53.659416 6 CGGAAGA 13620 0.0 53.59143 4 TCGGAAG 13680 0.0 53.56554 3 GATCGGA 14185 0.0 51.775513 1 ATCGGAA 14175 0.0 51.53252 2 GGAAGAG 15900 0.0 46.17543 5 GCCGTCT 8445 0.0 43.563515 50-51 ATGCCGT 8525 0.0 43.20902 48-49 GATCTCG 8310 0.0 42.491276 40-41 GTATGCC 8665 0.0 42.453312 46-47 TATGCCG 8555 0.0 42.30795 48-49 TGCCGTC 8575 0.0 42.26604 50-51 TCGTATG 8730 0.0 42.189564 44-45 TCGATCT 8370 0.0 42.156937 38-39 TCTCGTA 8495 0.0 41.595226 42-43 GAGCACA 17695 0.0 41.46316 9 CTCGATC 8390 0.0 41.320595 38-39 ACTCGAT 8875 0.0 41.06915 36-37 CGTATGC 8825 0.0 40.957054 46-47 TTACTCG 9025 0.0 40.806194 34-35 >>END_MODULE