FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n01_30521-1.341000000356df.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n01_30521-1.341000000356df.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3070365
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT685622.2330244124069942TruSeq Adapter, Index 27 (97% over 39bp)
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC37690.12275413509468744No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA36450.11871552730701398No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGAGC136200.053.6594166
CGGAAGA136200.053.591434
TCGGAAG136800.053.565543
GATCGGA141850.051.7755131
ATCGGAA141750.051.532522
GGAAGAG159000.046.175435
GCCGTCT84450.043.56351550-51
ATGCCGT85250.043.2090248-49
GATCTCG83100.042.49127640-41
GTATGCC86650.042.45331246-47
TATGCCG85550.042.3079548-49
TGCCGTC85750.042.2660450-51
TCGTATG87300.042.18956444-45
TCGATCT83700.042.15693738-39
TCTCGTA84950.041.59522642-43
GAGCACA176950.041.463169
CTCGATC83900.041.32059538-39
ACTCGAT88750.041.0691536-37
CGTATGC88250.040.95705446-47
TTACTCG90250.040.80619434-35
Produced by FastQC (version 0.11.2)