FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n01_30484-1.34100000035806.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n01_30484-1.34100000035806.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8394700
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT3248393.8695724683431214TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG480400.073.263753
CGGAAGA480350.073.160434
GAAGAGC490900.071.636936
GATCGGA493850.071.477571
ATCGGAA509400.069.1346742
GGAAGAG558300.063.167675
AGAGCAC761350.046.326988
GAGCACA765150.046.09079
AAGAGCA766650.045.9385767
GCCGTCT397450.044.11984350-51
ATGCCGT397650.043.98220448-49
CGATCGC652.5640857E-643.9135781
CGTCTTC400650.043.85729652-53
TTCGCGC652.593386E-643.841122
TGCCGTC397850.043.69938750-51
CCGTCTT398150.043.67333652-53
TATGCCG397800.043.6133748-49
TCTCGTA398600.043.4428142-43
TCGTATG405100.043.40320644-45
CGTATGC401600.043.2563546-47
Produced by FastQC (version 0.11.2)