##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n01_30423-1.3410000003574b.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2610328 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.69214520167581 33.0 33.0 33.0 33.0 33.0 2 32.702580671854264 33.0 33.0 33.0 33.0 33.0 3 32.735430949673756 33.0 33.0 33.0 33.0 33.0 4 36.6620980198657 37.0 37.0 37.0 37.0 37.0 5 36.63753712177167 37.0 37.0 37.0 37.0 37.0 6 36.53058581143826 37.0 37.0 37.0 37.0 37.0 7 36.410225841350204 37.0 37.0 37.0 37.0 37.0 8 36.34794784410235 37.0 37.0 37.0 37.0 37.0 9 36.39116118740633 37.0 37.0 37.0 37.0 37.0 10-11 36.31061881878446 37.0 37.0 37.0 35.0 37.0 12-13 36.29517937975611 37.0 37.0 37.0 35.0 37.0 14-15 38.08274975405389 40.0 37.0 40.0 35.0 40.0 16-17 38.09153428228177 40.0 37.0 40.0 37.0 40.0 18-19 38.100691369054005 40.0 37.0 40.0 37.0 40.0 20-21 38.07002051083236 40.0 37.0 40.0 37.0 40.0 22-23 37.8348370396364 40.0 37.0 40.0 33.0 40.0 24-25 37.929067917901506 40.0 37.0 40.0 33.0 40.0 26-27 37.85406565764915 40.0 37.0 40.0 33.0 40.0 28-29 37.626726028299885 40.0 37.0 40.0 33.0 40.0 30-31 37.59838725248321 40.0 37.0 40.0 33.0 40.0 32-33 37.48665799853505 40.0 37.0 40.0 33.0 40.0 34-35 37.4743302374261 40.0 37.0 40.0 33.0 40.0 36-37 37.35833695995292 40.0 37.0 40.0 33.0 40.0 38-39 37.02470800604368 38.5 37.0 40.0 33.0 40.0 40-41 36.86827536616088 37.0 37.0 40.0 33.0 40.0 42-43 36.654122393814106 37.0 37.0 40.0 33.0 40.0 44-45 36.46520571361147 37.0 37.0 40.0 33.0 40.0 46-47 36.58215442657014 37.0 37.0 40.0 33.0 40.0 48-49 36.145786659760766 37.0 37.0 40.0 33.0 40.0 50-51 36.11431398659479 37.0 37.0 40.0 33.0 40.0 52-53 36.125977080274964 37.0 37.0 40.0 33.0 40.0 54-55 35.67052971887058 37.0 35.0 40.0 30.0 40.0 56-57 35.47575477104793 37.0 33.0 40.0 27.0 40.0 58-59 35.35420797692856 37.0 33.0 40.0 27.0 40.0 60-61 35.00805588416475 37.0 33.0 38.5 27.0 40.0 62-63 34.81552184246577 37.0 33.0 37.0 27.0 40.0 64-65 34.508920718009385 37.0 33.0 37.0 27.0 40.0 66-67 33.67120875231005 37.0 33.0 37.0 27.0 40.0 68-69 33.56699694444529 37.0 33.0 37.0 27.0 37.0 70-71 33.28517546453932 37.0 33.0 37.0 27.0 37.0 72-73 32.39251484870867 35.0 33.0 37.0 22.0 37.0 74-75 31.308661785032378 33.0 30.0 37.0 18.5 37.0 76-77 28.69359501947648 33.0 27.0 33.0 15.0 35.0 78-79 30.801227661811083 33.0 30.0 37.0 15.0 37.0 80-81 31.17968508172153 33.0 33.0 37.0 15.0 37.0 82-83 31.00294848003776 33.0 33.0 37.0 15.0 37.0 84-85 30.87924410265683 33.0 33.0 37.0 15.0 37.0 86-87 30.7246547177213 33.0 33.0 37.0 15.0 37.0 88-89 30.6351259305344 33.0 33.0 37.0 15.0 37.0 90-91 30.519400627047638 33.0 33.0 37.0 15.0 37.0 92-93 30.309496354481123 33.0 27.0 37.0 15.0 37.0 94-95 30.16765019568422 33.0 27.0 37.0 15.0 37.0 96-97 30.02374931426242 33.0 27.0 37.0 15.0 37.0 98-99 29.727105942241742 33.0 27.0 37.0 10.5 37.0 100-101 28.16292109650588 33.0 24.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1300628214844366 2306 2 0.14594177139700548 2306 3 0.1256208300656283 2306 4 0.19765422068258687 2306 5 0.1914179954693438 2306 6 0.25585787187913667 2306 7 0.21060653855118971 2306 8 0.2425825098867449 2306 9 0.2396275636660974 2306 10-11 0.34170030833880105 2306 12-13 0.29330244972269526 2306 14-15 0.6114235787472424 2306 16-17 0.5960410670553884 2306 18-19 0.6165519795544512 2306 20-21 0.6427327173753099 2306 22-23 0.5728553898851416 2306 24-25 0.6886175086521362 2306 26-27 0.6560625178308683 2306 28-29 0.6210209859745959 2306 30-31 0.6749523183943325 2306 32-33 0.635749674844881 2306 34-35 0.7176447426379866 2306 36-37 0.6110017814567072 2306 38-39 0.8048745864394107 2306 40-41 0.7003133329514313 2306 42-43 0.8390351275814822 2306 44-45 0.9061485661630257 2306 46-47 0.7698579646391366 2306 48-49 0.6437857613299229 2306 50-51 0.606573425821189 2306 52-53 0.6311481050882435 2306 54-55 0.5575083961387861 2306 56-57 0.6870536835296903 2306 58-59 0.7118206123283528 2306 60-61 0.5469386088152035 2306 62-63 0.782460917029212 2306 64-65 0.5854057954807175 2306 66-67 0.3250224405799855 2306 68-69 0.696369994213093 2306 70-71 0.8897134111026546 2306 72-73 0.37197134250667574 2306 74-75 0.2304731142581069 2306 76-77 0.07684261462933151 2306 78-79 0.5806634175513921 2306 80-81 0.7188964515614416 2306 82-83 0.6660475773016543 2306 84-85 0.6165951958019633 2306 86-87 0.39444549956636266 2306 88-89 0.5778978705463409 2306 90-91 0.6966132670196004 2306 92-93 0.49556400301679915 2306 94-95 0.5203690813266029 2306 96-97 0.6828861044300893 2306 98-99 0.6765441418370415 2306 100-101 0.4472551821314532 2307 1 0.045043124185880856 2307 2 0.08335656657204993 2307 3 0.11053150774632314 2307 4 0.14035472953538175 2307 5 0.11638667981463868 2307 6 0.18863277225776187 2307 7 0.2703304217207503 2307 8 0.24612582479242917 2307 9 0.24296624513162612 2307 10-11 0.2898314056705473 2307 12-13 0.2715479999206636 2307 14-15 0.5148575193843854 2307 16-17 0.4083452112194763 2307 18-19 0.4306364701129368 2307 20-21 0.4968079728301831 2307 22-23 0.44946231209890186 2307 24-25 0.47379016678733166 2307 26-27 0.38383726925621175 2307 28-29 0.43190156232449795 2307 30-31 0.5830007963045603 2307 32-33 0.6387498422399958 2307 34-35 0.5364437819602372 2307 36-37 0.6336767270887549 2307 38-39 0.6272327226224377 2307 40-41 0.6317465898220789 2307 42-43 0.6077301833254793 2307 44-45 0.4670060902189803 2307 46-47 0.4924509150348513 2307 48-49 0.6360222807030738 2307 50-51 0.37493847911099465 2307 52-53 0.5347301123826895 2307 54-55 0.3539952974707177 2307 56-57 0.3747103504658966 2307 58-59 0.26079609745501386 2307 60-61 0.5340756061156 2307 62-63 0.45904574450585045 2307 64-65 0.3885207632997165 2307 66-67 0.47714009523250667 2307 68-69 0.6682891083240747 2307 70-71 0.5637779135490391 2307 72-73 0.3978828302095181 2307 74-75 0.31882067617656773 2307 76-77 0.09922710135759516 2307 78-79 0.40798435797150034 2307 80-81 0.6106414605624764 2307 82-83 0.5467418485583515 2307 84-85 0.5114008401384069 2307 86-87 0.5157282285040132 2307 88-89 0.597560590660283 2307 90-91 0.7361699394883523 2307 92-93 0.8050878632049923 2307 94-95 0.8170820724645935 2307 96-97 0.8455836980543232 2307 98-99 0.6821192504665454 2307 100-101 0.4530948601653826 2201 1 0.15838020148726883 2201 2 0.11484980894083208 2201 3 0.11506818014449749 2201 4 0.12382464836984752 2201 5 0.24109779339091375 2201 6 0.21609431569703474 2201 7 0.21775540369423396 2201 8 0.14732435678717337 2201 9 0.18624166251473184 2201 10-11 0.21421133985067087 2201 12-13 0.2739509571932004 2201 14-15 0.48502826281627165 2201 16-17 0.529924218189727 2201 18-19 0.4268838354687503 2201 20-21 0.4007439925761531 2201 22-23 0.41644374941285633 2201 24-25 0.36072579826994655 2201 26-27 0.4090142988566683 2201 28-29 0.3982948268797557 2201 30-31 0.49953457521631606 2201 32-33 0.5586578145808616 2201 34-35 0.6083892124703425 2201 36-37 0.4710221150747742 2201 38-39 0.4980860468962902 2201 40-41 0.5391723575666347 2201 42-43 0.5707735209112101 2201 44-45 0.6509770536667574 2201 46-47 0.4705065245158764 2201 48-49 0.5564340709669011 2201 50-51 0.6083442651593955 2201 52-53 0.7549707663291088 2201 54-55 0.6226169163440147 2201 56-57 0.6101002084724385 2201 58-59 0.5808608773466659 2201 60-61 0.7039691341352778 2201 62-63 0.5525501910456754 2201 64-65 0.3822265058996024 2201 66-67 0.40922058592343546 2201 68-69 0.654909622495687 2201 70-71 0.5388106154922028 2201 72-73 0.2697056573213885 2201 74-75 0.2091732562789943 2201 76-77 0.15377463588530205 2201 78-79 0.5360257542404732 2201 80-81 0.6477708770581216 2201 82-83 0.6248711818547079 2201 84-85 0.5938571142893103 2201 86-87 0.5637590630853389 2201 88-89 0.6865399202588911 2201 90-91 0.6442641904174131 2201 92-93 0.5819109676478327 2201 94-95 0.45387441628103886 2201 96-97 0.3939941293964182 2201 98-99 0.5824309011404587 2201 100-101 0.3388610794047544 1101 1 0.09469956394299572 1101 2 0.08895156713349905 1101 3 0.09169075903980684 1101 4 0.14033961374845205 1101 5 0.18114055161549203 1101 6 0.08550423624332382 1101 7 0.06039157587586175 1101 8 0.19549117768126933 1101 9 0.16455741923958556 1101 10-11 0.18456776475255054 1101 12-13 0.21876313211901532 1101 14-15 0.3787609122307387 1101 16-17 0.44805438347898274 1101 18-19 0.39337227638900885 1101 20-21 0.35191505738357876 1101 22-23 0.4502175615957569 1101 24-25 0.4262531762973154 1101 26-27 0.44701208117263036 1101 28-29 0.5099049612171598 1101 30-31 0.5145797266251009 1101 32-33 0.5184243993474453 1101 34-35 0.6155986799070803 1101 36-37 0.5947816479252168 1101 38-39 0.6567252424673029 1101 40-41 0.7079377138319884 1101 42-43 0.7123762363775725 1101 44-45 0.5207459518447521 1101 46-47 0.6001822081688317 1101 48-49 0.7638413749651178 1101 50-51 0.72278134834648 1101 52-53 0.8097796705698386 1101 54-55 0.7402020339291298 1101 56-57 0.7567116590611604 1101 58-59 0.6222998591265636 1101 60-61 0.5313218336470626 1101 62-63 0.652986149436181 1101 64-65 0.6227873885059338 1101 66-67 0.444615780409535 1101 68-69 0.6711184597272535 1101 70-71 0.5894952656086687 1101 72-73 0.20646676169613443 1101 74-75 0.06429187844306838 1101 76-77 0.03598210900186416 1101 78-79 0.45959343348997095 1101 80-81 0.3999057968066779 1101 82-83 0.325032123492921 1101 84-85 0.2963404417726352 1101 86-87 0.34913930017011907 1101 88-89 0.4103963770698975 1101 90-91 0.2528140404445338 1101 92-93 0.3615615228665696 1101 94-95 0.4831630023878084 1101 96-97 0.3519139736798991 1101 98-99 0.19227705928206973 1101 100-101 0.42066609870977345 2304 1 0.11448720873063678 2304 2 0.15217550535743385 2304 3 0.14043593278497468 2304 4 0.13165235506119188 2304 5 0.14928726976221185 2304 6 0.15258917832826313 2304 7 0.1351985256828101 2304 8 0.11103171822180968 2304 9 0.11002073970290382 2304 10-11 0.14692297710775648 2304 12-13 0.15119556455145045 2304 14-15 0.3155369740067968 2304 16-17 0.3406702135948123 2304 18-19 0.3624842455009869 2304 20-21 0.38449227746080084 2304 22-23 0.42681022568842764 2304 24-25 0.37509487513901973 2304 26-27 0.42818968735023333 2304 28-29 0.44354395735615526 2304 30-31 0.40960868415406537 2304 32-33 0.4631637815868217 2304 34-35 0.46719134399974394 2304 36-37 0.5009109915545622 2304 38-39 0.5961557443978052 2304 40-41 0.48786049375477347 2304 42-43 0.46261754911888175 2304 44-45 0.4973386159374158 2304 46-47 0.6110412815279034 2304 48-49 0.7860421471658867 2304 50-51 0.6549724680376059 2304 52-53 0.5609862342763989 2304 54-55 0.6750475937746927 2304 56-57 0.7376479524974542 2304 58-59 0.7924253417520504 2304 60-61 0.7487446135698477 2304 62-63 0.5996560336060668 2304 64-65 0.6495731027916563 2304 66-67 0.5633416491354026 2304 68-69 0.6600180736268655 2304 70-71 0.5091381227515299 2304 72-73 0.30106135629072384 2304 74-75 0.38813357728476916 2304 76-77 0.2063489043686566 2304 78-79 0.5080972944938296 2304 80-81 0.4189695033703842 2304 82-83 0.4256595366203335 2304 84-85 0.27100260393480013 2304 86-87 0.4196026206334409 2304 88-89 0.37781915699267543 2304 90-91 0.48109589372638695 2304 92-93 0.40186075066580074 2304 94-95 0.35381937304417477 2304 96-97 0.2705915798575056 2304 98-99 0.32137937588438703 2304 100-101 0.36371629175996745 2202 1 0.1467383275932903 2202 2 0.14276269034560585 2202 3 0.10025763103984531 2202 4 0.11748383441077692 2202 5 0.09781354685352994 2202 6 0.11565657070946855 2202 7 0.17201592453641723 2202 8 0.12228852071687868 2202 9 0.14557580431473838 2202 10-11 0.16224649799118396 2202 12-13 0.1651126323632397 2202 14-15 0.2890709964064868 2202 16-17 0.2886332837919028 2202 18-19 0.1969105878341182 2202 20-21 0.17666256896055188 2202 22-23 0.3153047534878297 2202 24-25 0.2813048001799672 2202 26-27 0.35281262675301406 2202 28-29 0.2940310272218625 2202 30-31 0.3607416134944117 2202 32-33 0.41485980994803384 2202 34-35 0.27214365965277665 2202 36-37 0.3738638785049915 2202 38-39 0.44131300651974925 2202 40-41 0.4716128216622195 2202 42-43 0.3426487633588522 2202 44-45 0.39851678737151275 2202 46-47 0.3832132515373843 2202 48-49 0.4696717583211978 2202 50-51 0.4335934320631267 2202 52-53 0.4812447247740508 2202 54-55 0.5547017905667957 2202 56-57 0.5733337164912271 2202 58-59 0.5233318621366578 2202 60-61 0.5967178022895681 2202 62-63 0.5970806646359748 2202 64-65 0.5063294496295825 2202 66-67 0.5926710176187697 2202 68-69 0.4953143068611894 2202 70-71 0.4630693953294269 2202 72-73 0.13069553178431903 2202 74-75 0.026805988280603543 2202 76-77 0.05901339832171004 2202 78-79 0.24152945422236982 2202 80-81 0.23233788404430555 2202 82-83 0.39345987335786603 2202 84-85 0.4247055517258147 2202 86-87 0.41173088466604213 2202 88-89 0.43786622430393507 2202 90-91 0.477613417540514 2202 92-93 0.44987594527799857 2202 94-95 0.5874434395079824 2202 96-97 0.5827375394386465 2202 98-99 0.518057242684069 2202 100-101 0.4330441370712741 1102 1 0.08963868564049449 1102 2 0.035415481843166674 1102 3 0.05801919516550669 1102 4 0.0486048297298467 1102 5 0.06927167878261287 1102 6 0.09510922216191631 1102 7 0.17575563962635243 1102 8 0.12378884558381742 1102 9 0.1476084189951763 1102 10-11 0.16164485144383178 1102 12-13 0.07585976344506662 1102 14-15 0.18979127071144575 1102 16-17 0.19520994822867266 1102 18-19 0.09835402168416607 1102 20-21 0.18162823367826775 1102 22-23 0.2635699936128617 1102 24-25 0.24026984235163695 1102 26-27 0.23733606923658357 1102 28-29 0.3737132217207062 1102 30-31 0.27481500105669454 1102 32-33 0.24409853749055088 1102 34-35 0.19230518131362118 1102 36-37 0.2915906089046416 1102 38-39 0.20175947153649787 1102 40-41 0.23478722573528898 1102 42-43 0.2917064272871954 1102 44-45 0.30568548088853476 1102 46-47 0.26109657279647536 1102 48-49 0.24763717945806718 1102 50-51 0.25700832090092973 1102 52-53 0.32264492441046855 1102 54-55 0.18135097810352363 1102 56-57 0.25417363208685373 1102 58-59 0.24553668866252565 1102 60-61 0.24472266798812115 1102 62-63 0.2707356849815383 1102 64-65 0.35292326644901806 1102 66-67 0.41405911774178605 1102 68-69 0.46516068077092143 1102 70-71 0.3082210544627131 1102 72-73 0.13391229710091324 1102 74-75 0.19643591369529645 1102 76-77 0.2702312859227405 1102 78-79 0.451333389209033 1102 80-81 0.43268359499614206 1102 82-83 0.4754129922747019 1102 84-85 0.3826049045627542 1102 86-87 0.5083110613278166 1102 88-89 0.5877330871176092 1102 90-91 0.5321668350797033 1102 92-93 0.5303983734886906 1102 94-95 0.46019436246116285 1102 96-97 0.4645104788084673 1102 98-99 0.4265914315774104 1102 100-101 0.4317189610762462 2305 1 0.003309664033984916 2305 2 0.022736580536346196 2305 3 0.048340538937992505 2305 4 0.04532541164826398 2305 5 0.10428013797163516 2305 6 0.025738601636923875 2305 7 0.04561309175024064 2305 8 0.0561553564186994 2305 9 -0.001139897731619044 2305 10-11 0.03595293062816296 2305 12-13 0.0016546735694760173 2305 14-15 0.02796596350585645 2305 16-17 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72-73 0.1638766217222738 1213 74-75 0.20107055911709892 1213 76-77 -2.0351615013859323 1213 78-79 -1.9295128239302386 1213 80-81 -1.4224256708736291 1213 82-83 -0.9729523227473393 1213 84-85 -0.7634807997346513 1213 86-87 -0.5644739808850225 1213 88-89 -0.6582173778810692 1213 90-91 -1.6711215645957225 1213 92-93 -0.8967937593259201 1213 94-95 -0.45482062077023855 1213 96-97 -0.4924506567312399 1213 98-99 -2.184414748805086 1213 100-101 -0.8133030032825843 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 4.0 9 13.0 10 14.0 11 15.0 12 16.0 13 66.0 14 228.0 15 663.0 16 1474.0 17 2524.0 18 3562.0 19 4564.0 20 6404.0 21 8546.0 22 11419.0 23 15502.0 24 20668.0 25 27472.0 26 37800.0 27 56695.0 28 90545.0 29 113414.0 30 79152.0 31 63946.0 32 84182.0 33 120850.0 34 187096.0 35 323485.0 36 569951.0 37 672014.0 38 108019.0 39 24.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.80464210993275 21.462505621892607 21.31280594224455 12.420046325930088 2 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23.001007153509885 20-21 26.252333040139014 25.530029176410014 30.366222175910462 17.851415607540506 22-23 21.897631255535703 34.052157430024124 25.58919798584699 18.461013328593186 24-25 22.59325264870928 22.906450836829702 31.20320511445305 23.297091400007968 26-27 20.49041346528099 22.99358548044537 27.367652647483382 29.14834840679026 28-29 24.75447529965583 29.515352093683244 25.239031263504053 20.491141343156873 30-31 18.431630048024616 25.852479075426537 30.58134839759601 25.134542478952838 32-33 18.98966336797521 28.903972987302744 26.5138135897098 25.592550055012243 34-35 19.857063939857365 23.94677986827709 30.842273461419406 25.353882730446138 36-37 23.776590528086892 26.385917785044636 26.249919550340035 23.587572136528436 38-39 23.8471071609857 30.909082202407745 24.12299234769617 21.120818288910385 40-41 24.822924348500667 30.06851637248235 23.898365146102496 21.210194132914484 42-43 21.059333794712202 30.386644556747783 29.16967319550326 19.384348453036758 44-45 18.96414437258638 26.821221419403134 29.00222214517747 25.212412062833018 46-47 24.363703653047022 26.223357272446723 25.211849755772004 24.201089318734248 48-49 19.847908614144398 29.85985402690285 30.53186969462575 19.760367664327006 50-51 24.07057980648214 25.30294874324498 28.655661958664343 21.97080949160854 52-53 18.922801791116136 26.160172184983928 24.59089592585441 30.326130098045528 54-55 24.39223360269816 26.051874457689273 30.050485069343928 19.50540687026864 56-57 20.117466510320515 25.420892288010766 28.66393194995978 25.797709251708945 58-59 18.894698665824706 24.716939416662804 29.60785538381249 26.780506533700006 60-61 22.966682611361755 24.68409293490369 31.339352471692738 21.009871982041815 62-63 18.71071850958195 24.70205929352413 31.120135225064143 25.467086971829772 64-65 24.721424321247767 26.34410394283772 29.427488330339052 19.506983405575458 66-67 20.10487473816763 35.2511636355554 24.44849461236546 20.195467013911507 68-69 19.558758438064654 33.39080382685273 26.477616973659252 20.572820761423362 70-71 19.152252801558703 36.19785149640658 25.275786967717263 19.37410873431746 72-73 21.00594561762187 35.63574804990257 24.457699507757276 18.900606824718288 74-75 22.375240738778473 33.469506868492196 24.648910838461717 19.50634155426761 76-77 21.56414141762531 31.687633124166958 25.05365205721149 21.69457340099624 78-79 21.55494436651316 32.51632565715488 25.064783441494125 20.86394653483784 80-81 21.112894667782772 32.56550580113514 25.033435302545907 21.288164228536182 82-83 20.41601668449329 31.686573488082725 25.937487549457387 21.9599222779666 84-85 21.082669054259306 30.866473536780592 25.79308820281358 22.25776920614652 86-87 19.295128931044157 31.46245495498948 27.031930417242105 22.210485696724263 88-89 19.02229196544645 31.681434411191873 27.12681866474298 22.1694549586187 90-91 20.439527720660536 30.592108036274706 25.859294110073332 23.109070132991423 92-93 20.554560959799066 30.831161201350742 24.97158807294991 23.642689765900286 94-95 20.876471824680344 31.851348557477206 24.877949517402648 22.394230100439803 96-97 20.332586817012295 33.10232715349648 23.78020459452437 22.784881434966856 98-99 20.577741156632044 30.692618709998847 25.390392059172957 23.339248074196146 100-101 21.712537874738132 31.435773985865207 24.859201966286644 21.992486173110017 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 248.0 1 160.0 2 103.5 3 225.5 4 249.0 5 171.0 6 159.0 7 169.0 8 185.5 9 195.5 10 199.5 11 195.0 12 198.0 13 200.5 14 187.0 15 193.5 16 205.5 17 222.5 18 237.5 19 255.5 20 276.0 21 313.0 22 364.5 23 406.5 24 483.5 25 600.0 26 775.5 27 1016.5 28 1314.0 29 1682.5 30 2285.5 31 3361.5 32 5689.5 33 9703.0 34 16128.5 35 27475.5 36 44983.0 37 68193.0 38 95047.5 39 116039.0 40 130879.5 41 140343.5 42 173040.0 43 212778.5 44 205893.5 45 181561.0 46 174287.5 47 162193.0 48 134210.0 49 111049.5 50 93872.0 51 85234.5 52 83013.0 53 82730.5 54 68908.5 55 42409.5 56 27435.5 57 21870.0 58 18892.0 59 15186.0 60 11526.0 61 8923.0 62 6633.0 63 4693.0 64 3420.5 65 2476.0 66 1779.5 67 1337.0 68 1047.0 69 825.0 70 652.0 71 487.5 72 282.0 73 152.5 74 103.0 75 76.0 76 50.5 77 31.0 78 20.0 79 12.0 80 8.5 81 5.0 82 5.0 83 4.0 84 5.5 85 5.0 86 2.0 87 1.0 88 1.5 89 1.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17024680423303126 2 0.006474282159176931 3 0.001800540008765182 4 0.0 5 6.895685139951762E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.9154680944310446E-4 12-13 0.0 14-15 0.0 16-17 0.0 18-19 3.830936188862089E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 1.1492808566586268E-4 40-41 1.1492808566586268E-4 42-43 0.0013982917089346626 44-45 4.7886702360776115E-4 46-47 0.006301890030678137 48-49 0.004635432788523128 50-51 0.008926081320048669 52-53 0.00429064853152554 54-55 0.020936066272131317 56-57 0.011780128780750925 58-59 0.021108458400630113 60-61 0.010132826219540226 62-63 0.006167807264067964 64-65 0.013599823470460416 66-67 0.03195000781510982 68-69 0.03344407292876604 70-71 0.03742824656518261 72-73 0.03229479207210741 74-75 0.03495729272336656 76-77 0.03532123166130847 78-79 0.018350184344649408 80-81 0.002413489798983116 82-83 0.0 84-85 0.006416818116344 86-87 0.02742950311225256 88-89 0.028942722906853083 90-91 0.02628022225559393 92-93 0.03633642975135692 94-95 0.03273534973382655 96-97 0.03348238229065466 98-99 0.03938202402150228 100-101 0.02831061843569084 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2610328.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.636859323023153 #Duplication Level Percentage of deduplicated Percentage of total 1 83.25783358190571 26.340163685704237 2 6.972198866672223 4.411569494341012 3 2.8353287106795144 2.6910268666288926 4 1.1524936032320747 1.4584511198454884 5 1.0111230871076935 1.5994379432543493 6 0.7279264922158534 1.3817584819040796 7 0.4251789195555565 0.9415927965565882 8 0.4166830876981181 1.0546035382230625 9 0.29628567263325406 0.8436193330072993 >10 2.3030420884033798 14.364469555772988 >50 0.3084293778856328 6.882049046015255 >100 0.2618372416990506 16.350977898859824 >500 0.021546578959571218 4.55987429054565 >1k 0.008988851663305876 4.509598555818361 >5k 4.905954173881185E-4 0.9443690512240706 >10k+ 6.132442716278486E-4 11.666438342298868 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT 224604 8.604435917631807 TruSeq Adapter, Index 6 (97% over 36bp) TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 28090 1.076109975451361 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 25608 0.9810261392438038 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 12127 0.4645776316233056 No Hit TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 10235 0.3920963189300348 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 8744 0.3349770603541011 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATATCGTAT 5242 0.20081767502015072 TruSeq Adapter, Index 6 (97% over 36bp) TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 5218 0.19989825033482383 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 5134 0.19668026393617968 No Hit TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 3523 0.13496388193361142 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 3195 0.12239841123414376 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 2904 0.11125038692455506 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.003984173636416573 0.0 0.0 2 0.004328957893414161 0.0 0.0 3 0.00471205151230037 0.0 0.0 4 0.004903598321743474 0.0 0.0 5 0.006052879178402101 0.0 0.0 6 0.006742447692397277 0.0 0.0 7 0.007700181739612799 0.0 0.0 8 0.008696225148716943 0.0 0.0 9 0.00988381536726419 0.0 0.0 10-11 0.015572755607724394 0.0 0.0 12-13 0.02051466329135649 0.0 0.0 14-15 0.0273145750265867 0.0 0.0 16-17 0.03430603357126001 0.0 0.0 18-19 0.04302141340092126 0.0 0.0 20-21 0.048844436407991645 0.0 0.0 22-23 0.05411197366767701 0.0 0.0 24-25 0.05947528433208393 0.0 0.0 26-27 0.06522168861537707 0.0 0.0 28-29 0.07211737375532884 0.0 0.0 30-31 0.12433303400951912 0.0 0.0 32-33 0.13486810852888986 0.0 0.0 34-35 0.14381334452988281 0.0 0.0 36-37 0.1526819618070986 0.0 0.0 38-39 0.16264239589814 0.0 0.0 40-41 0.173809574888673 0.0 0.0 42-43 0.18547477558375808 0.0 0.0 44-45 0.19828925713550175 0.0 0.0 46-47 0.2114102135823544 0.0 0.0 48-49 0.2275576096184081 0.0 0.0 50-51 0.24418387267806957 0.0 0.0 52-53 0.26040788743790055 0.0 0.0 54-55 0.278451596887441 0.0 0.0 56-57 0.29956005528807106 0.0 0.0 58-59 0.32241158965463346 0.0 0.0 60-61 0.34844280105795133 0.0 0.0 62-63 0.378592268864296 0.0 0.0 64-65 0.41044650327468424 0.0 0.0 66-67 0.4447525368459443 0.0 0.0 68-69 0.4817785351112963 0.0 0.0 70-71 0.5211030950899658 0.0 0.0 72-73 0.5664996889279814 0.0 0.0 74-75 0.6164359421497988 0.0 0.0 76-77 0.6733828085972338 0.0 0.0 78-79 0.7402709544547659 0.0 0.0 80-81 0.814073940133194 0.0 0.0 82-83 0.8933168551998063 0.0 0.0 84-85 0.9836503305331744 0.0 0.0 86-87 1.0868749061420633 0.0 0.0 88-89 1.201611444998483 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 30130 0.0 81.833176 3 CGGAAGA 30115 0.0 81.52545 4 GAAGAGC 30210 0.0 81.221924 6 GATCGGA 30450 0.0 81.19894 1 ATCGGAA 30610 0.0 80.59802 2 GGAAGAG 31830 0.0 77.0881 5 GAGCACA 38160 0.0 64.06425 9 AGAGCAC 38425 0.0 63.82016 8 AAGAGCA 38545 0.0 63.52291 7 CGACGCG 25 0.004990449 57.090797 1 GCCGTCT 26385 0.0 45.84728 50-51 ATGCCGT 26200 0.0 45.84377 48-49 TCTCGTA 25705 0.0 45.69569 42-43 TCGTATG 26620 0.0 45.67864 44-45 GTATGCC 26585 0.0 45.445717 46-47 AATCTCG 25860 0.0 45.41832 40-41 CTGCTTG 26735 0.0 45.415436 58-59 TGCCGTC 26395 0.0 45.119022 50-51 TATGCCG 26360 0.0 45.07895 48-49 CACTCCG 27240 0.0 45.056244 30-31 >>END_MODULE