FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n01_30423-1.3410000003574b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n01_30423-1.3410000003574b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2610328
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT2246048.604435917631807TruSeq Adapter, Index 6 (97% over 36bp)
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA280901.076109975451361No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC256080.9810261392438038No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC121270.4645776316233056No Hit
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT102350.3920963189300348No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT87440.3349770603541011No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATATCGTAT52420.20081767502015072TruSeq Adapter, Index 6 (97% over 36bp)
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT52180.19989825033482383No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC51340.19668026393617968No Hit
TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT35230.13496388193361142No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC31950.12239841123414376No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG29040.11125038692455506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG301300.081.8331763
CGGAAGA301150.081.525454
GAAGAGC302100.081.2219246
GATCGGA304500.081.198941
ATCGGAA306100.080.598022
GGAAGAG318300.077.08815
GAGCACA381600.064.064259
AGAGCAC384250.063.820168
AAGAGCA385450.063.522917
CGACGCG250.00499044957.0907971
GCCGTCT263850.045.8472850-51
ATGCCGT262000.045.8437748-49
TCTCGTA257050.045.6956942-43
TCGTATG266200.045.6786444-45
GTATGCC265850.045.44571746-47
AATCTCG258600.045.4183240-41
CTGCTTG267350.045.41543658-59
TGCCGTC263950.045.11902250-51
TATGCCG263600.045.0789548-49
CACTCCG272400.045.05624430-31
Produced by FastQC (version 0.11.2)