##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n01_30245-1.34100000035771.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2174707 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.71755643403916 33.0 33.0 33.0 33.0 33.0 2 32.74590232155412 33.0 33.0 33.0 33.0 33.0 3 32.77366698134507 33.0 33.0 33.0 33.0 33.0 4 36.7242865360713 37.0 37.0 37.0 37.0 37.0 5 36.69232177024307 37.0 37.0 37.0 37.0 37.0 6 36.57881268603081 37.0 37.0 37.0 37.0 37.0 7 36.541071509863166 37.0 37.0 37.0 37.0 37.0 8 36.47476786528024 37.0 37.0 37.0 37.0 37.0 9 36.4956635537569 37.0 37.0 37.0 37.0 37.0 10-11 36.44742096291593 37.0 37.0 37.0 37.0 37.0 12-13 36.4278751114518 37.0 37.0 37.0 37.0 37.0 14-15 38.328652549515866 40.0 37.0 40.0 37.0 40.0 16-17 38.31844174870454 40.0 37.0 40.0 37.0 40.0 18-19 38.336892280201425 40.0 37.0 40.0 37.0 40.0 20-21 38.27657725845367 40.0 37.0 40.0 37.0 40.0 22-23 38.1797462830625 40.0 37.0 40.0 37.0 40.0 24-25 38.17143964681219 40.0 37.0 40.0 37.0 40.0 26-27 38.09938281340889 40.0 37.0 40.0 37.0 40.0 28-29 37.98372332456741 40.0 37.0 40.0 35.0 40.0 30-31 37.93572605413051 40.0 37.0 40.0 33.0 40.0 32-33 37.82799682899811 40.0 37.0 40.0 33.0 40.0 34-35 37.76978921758195 40.0 37.0 40.0 33.0 40.0 36-37 37.70169360746068 40.0 37.0 40.0 33.0 40.0 38-39 37.58549818435311 40.0 37.0 40.0 33.0 40.0 40-41 37.49149908470429 40.0 37.0 40.0 33.0 40.0 42-43 37.389259104789744 40.0 37.0 40.0 33.0 40.0 44-45 37.28265899728101 40.0 37.0 40.0 33.0 40.0 46-47 37.22276196287592 40.0 37.0 40.0 33.0 40.0 48-49 37.01702252303414 38.5 37.0 40.0 33.0 40.0 50-51 36.885447786759315 37.0 37.0 40.0 33.0 40.0 52-53 36.7931684590154 37.0 37.0 40.0 33.0 40.0 54-55 36.61536105783446 37.0 37.0 40.0 33.0 40.0 56-57 36.43866139208639 37.0 37.0 40.0 33.0 40.0 58-59 36.254065720117694 37.0 37.0 40.0 33.0 40.0 60-61 36.02305873848753 37.0 37.0 40.0 33.0 40.0 62-63 35.82308651234396 37.0 37.0 40.0 30.0 40.0 64-65 35.56714122868046 37.0 35.0 40.0 27.0 40.0 66-67 35.22007010599589 37.0 33.0 38.5 27.0 40.0 68-69 34.98284067692798 37.0 33.0 37.0 27.0 40.0 70-71 34.72443920031526 37.0 33.0 37.0 27.0 40.0 72-73 34.33861458118266 37.0 33.0 37.0 27.0 40.0 74-75 33.86690321960614 37.0 33.0 37.0 27.0 38.5 76-77 31.19094894162754 33.0 30.0 35.0 24.5 37.0 78-79 33.522656385434914 37.0 33.0 37.0 27.0 37.0 80-81 33.86266011927124 37.0 33.0 37.0 27.0 37.0 82-83 33.78183819705367 37.0 33.0 37.0 27.0 37.0 84-85 33.634848970459004 37.0 33.0 37.0 27.0 37.0 86-87 33.45245796330264 37.0 33.0 37.0 27.0 37.0 88-89 33.30129507101417 37.0 33.0 37.0 27.0 37.0 90-91 33.145832978879454 37.0 33.0 37.0 27.0 37.0 92-93 32.991497705208104 37.0 33.0 37.0 27.0 37.0 94-95 32.89277130206506 37.0 33.0 37.0 27.0 37.0 96-97 32.76290415214555 33.0 33.0 37.0 27.0 37.0 98-99 32.47647936020806 33.0 33.0 37.0 27.0 37.0 100-101 30.912942525130973 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1231746624780925 2306 2 0.10136837758445694 2306 3 0.13628378884710202 2306 4 0.13631417591898298 2306 5 0.21606589401093856 2306 6 0.18582983595921831 2306 7 0.22049646680744672 2306 8 0.3296996010040303 2306 9 0.2148231939344427 2306 10-11 0.2637790856966902 2306 12-13 0.2912267132738364 2306 14-15 0.5714992757551087 2306 16-17 0.5592591322110536 2306 18-19 0.6115830211859858 2306 20-21 0.5461210881826801 2306 22-23 0.49318207258831137 2306 24-25 0.48551271111384864 2306 26-27 0.498072357939364 2306 28-29 0.4775602627916342 2306 30-31 0.5887155298579572 2306 32-33 0.6953086466045733 2306 34-35 0.7152130052567927 2306 36-37 0.6248895326988304 2306 38-39 0.6583288057805419 2306 40-41 0.590766644527811 2306 42-43 0.6350081690417753 2306 44-45 0.6606442370771433 2306 46-47 0.7177496898206712 2306 48-49 0.7596975442208489 2306 50-51 0.6213854142567499 2306 52-53 0.7744584762933826 2306 54-55 0.7813579564283586 2306 56-57 0.7657186524505093 2306 58-59 0.8566717816410545 2306 60-61 0.7616160293267171 2306 62-63 0.9021012764915284 2306 64-65 0.6577761462859257 2306 66-67 0.6334448179801413 2306 68-69 0.7567799701377211 2306 70-71 0.73480080074836 2306 72-73 0.7378246943786522 2306 74-75 0.8298304028748262 2306 76-77 0.26154743237740874 2306 78-79 0.6431976222540428 2306 80-81 0.74302790658097 2306 82-83 0.8812025442817344 2306 84-85 0.6753299600871756 2306 86-87 0.6464688416817381 2306 88-89 0.8072284167732491 2306 90-91 0.7592076453770105 2306 92-93 0.7527829700244624 2306 94-95 0.991598530334322 2306 96-97 1.0187004920043492 2306 98-99 0.7242249481190015 2306 100-101 0.600668999559435 2307 1 0.10258529083677104 2307 2 0.012538222924156628 2307 3 0.08422028563330741 2307 4 0.12689874735424667 2307 5 0.1821740976801678 2307 6 0.13750171260563349 2307 7 0.1619169765659123 2307 8 0.20300401602282392 2307 9 0.21828695237870477 2307 10-11 0.21959728667311396 2307 12-13 0.27349346423681453 2307 14-15 0.45230137847951113 2307 16-17 0.4159627370487158 2307 18-19 0.4572004915944703 2307 20-21 0.47375212795414967 2307 22-23 0.476822736187529 2307 24-25 0.4434213432892733 2307 26-27 0.4574441748758886 2307 28-29 0.39134826930118294 2307 30-31 0.5754687173118569 2307 32-33 0.597052233646366 2307 34-35 0.5660173324669344 2307 36-37 0.5078534843221263 2307 38-39 0.4916483824257867 2307 40-41 0.511440605166122 2307 42-43 0.5955311573456541 2307 44-45 0.6211885921518956 2307 46-47 0.5085880282468977 2307 48-49 0.6027480751704744 2307 50-51 0.4681303476977945 2307 52-53 0.5925751564240471 2307 54-55 0.457915681833164 2307 56-57 0.46528224347295577 2307 58-59 0.457238440112242 2307 60-61 0.4838327878997717 2307 62-63 0.46577161941356593 2307 64-65 0.3781378008349989 2307 66-67 0.437943254986223 2307 68-69 0.4649435400860895 2307 70-71 0.3457484902101484 2307 72-73 0.221833276210468 2307 74-75 0.3440223291623923 2307 76-77 0.03776299675285344 2307 78-79 0.2103858722862313 2307 80-81 0.29246358967370867 2307 82-83 0.38805922886136557 2307 84-85 0.28857004063697644 2307 86-87 0.2867329115540045 2307 88-89 0.28938160188558726 2307 90-91 0.5862556357297635 2307 92-93 0.5424973577640344 2307 94-95 0.5774449471561169 2307 96-97 0.6231901481456887 2307 98-99 0.5835953015780788 2307 100-101 0.48325917904031357 2201 1 0.06492947085943968 2201 2 0.044075466821773546 2201 3 0.06893778603803469 2201 4 0.09322904073650307 2201 5 0.13276679820001647 2201 6 0.17256637902047345 2201 7 0.1787617232066765 2201 8 0.1349549810723758 2201 9 0.14748037601117403 2201 10-11 0.19242570032231043 2201 12-13 0.24451319693697116 2201 14-15 0.3611487153744477 2201 16-17 0.3422012023231247 2201 18-19 0.3443989698201406 2201 20-21 0.35956958698675834 2201 22-23 0.45844452310315376 2201 24-25 0.39987989022863957 2201 26-27 0.45035560257362306 2201 28-29 0.38618350035545035 2201 30-31 0.4959119385807256 2201 32-33 0.5326668793751423 2201 34-35 0.4324078852343689 2201 36-37 0.5780358813864979 2201 38-39 0.5004341108448358 2201 40-41 0.5371403335491891 2201 42-43 0.548314781183187 2201 44-45 0.44740817015632217 2201 46-47 0.46836039167394716 2201 48-49 0.7502670973495214 2201 50-51 0.6637300634893535 2201 52-53 0.6824821591042394 2201 54-55 0.5595593240183305 2201 56-57 0.6593116176747458 2201 58-59 0.576256122194529 2201 60-61 0.6407464281062687 2201 62-63 0.5979685686697707 2201 64-65 0.5945139633014946 2201 66-67 0.49840725225206484 2201 68-69 0.6482422467577891 2201 70-71 0.5504707574020671 2201 72-73 0.4144497027389846 2201 74-75 0.3636889238775538 2201 76-77 0.17415336964152672 2201 78-79 0.3180343172343427 2201 80-81 0.2350696731734203 2201 82-83 0.3745206538609125 2201 84-85 0.3693189658613818 2201 86-87 0.4418176344507785 2201 88-89 0.6013741189291011 2201 90-91 0.5316507394298711 2201 92-93 0.5368469161616716 2201 94-95 0.5640817539483365 2201 96-97 0.4936410142619323 2201 98-99 0.6459340852378901 2201 100-101 0.5145369478241513 1101 1 0.0717326187094045 1101 2 0.11126429677172922 1101 3 0.12167960797120969 1101 4 0.13847725417442547 1101 5 0.17520786120736176 1101 6 0.11136167041190248 1101 7 0.15571048351421268 1101 8 0.2108820300133729 1101 9 0.12927397823304432 1101 10-11 0.19582359540482486 1101 12-13 0.1856933463676782 1101 14-15 0.41729211123261223 1101 16-17 0.4528534262414894 1101 18-19 0.3348756083959401 1101 20-21 0.3121454350374009 1101 22-23 0.4164096817542031 1101 24-25 0.41856163317900297 1101 26-27 0.4422666024675195 1101 28-29 0.47762655148671485 1101 30-31 0.4614034280627166 1101 32-33 0.3581590504111105 1101 34-35 0.5049624037435976 1101 36-37 0.503076368016039 1101 38-39 0.48401272615950575 1101 40-41 0.46132423075226114 1101 42-43 0.5788462693640426 1101 44-45 0.663374423940688 1101 46-47 0.5377809236423161 1101 48-49 0.5403976536602073 1101 50-51 0.43999912860641643 1101 52-53 0.5472006656955628 1101 54-55 0.5355814032691129 1101 56-57 0.6146303332243193 1101 58-59 0.5685342318426123 1101 60-61 0.43101716767752407 1101 62-63 0.535029769893157 1101 64-65 0.5067795726301085 1101 66-67 0.6153971536358469 1101 68-69 0.6543359588802389 1101 70-71 0.5207062110653027 1101 72-73 0.5137825156129168 1101 74-75 0.33420822928111704 1101 76-77 0.24112824585816028 1101 78-79 0.5757951258671525 1101 80-81 0.6218962804209767 1101 82-83 0.5960303423830027 1101 84-85 0.5444440658678147 1101 86-87 0.4984222409811494 1101 88-89 0.5583756144753806 1101 90-91 0.5339238768319277 1101 92-93 0.5414243643151693 1101 94-95 0.6455425564626509 1101 96-97 0.6575317038473898 1101 98-99 0.526888291781269 1101 100-101 0.6227673316552149 2304 1 0.06951483470703579 2304 2 0.07933044914972953 2304 3 0.05979790159742748 2304 4 0.12594802213231304 2304 5 0.10436244541715922 2304 6 0.10468951443149876 2304 7 0.14779509560813864 2304 8 0.21131488301367796 2304 9 0.07895237604773087 2304 10-11 0.16017108125935664 2304 12-13 0.16725370007041107 2304 14-15 0.25114110737165163 2304 16-17 0.3199632157707839 2304 18-19 0.36555230191974175 2304 20-21 0.3382968800685049 2304 22-23 0.28482327370002736 2304 24-25 0.30269679324602095 2304 26-27 0.37015571543709314 2304 28-29 0.3337493963976925 2304 30-31 0.2687930641082019 2304 32-33 0.3889561616868775 2304 34-35 0.32492828176438593 2304 36-37 0.3312058825516928 2304 38-39 0.467538637492261 2304 40-41 0.32988337268356815 2304 42-43 0.42564487483245017 2304 44-45 0.4399920809439237 2304 46-47 0.29237677743853396 2304 48-49 0.5501766238027557 2304 50-51 0.34125241342549373 2304 52-53 0.40124422509966706 2304 54-55 0.38972151384370335 2304 56-57 0.44117669785578784 2304 58-59 0.35425608563955535 2304 60-61 0.41322716249558766 2304 62-63 0.4650558459414853 2304 64-65 0.43183235936086817 2304 66-67 0.32922400448020284 2304 68-69 0.6801364566792643 2304 70-71 0.518629095247654 2304 72-73 0.44880737482720434 2304 74-75 0.38102397529440424 2304 76-77 0.17620101904282492 2304 78-79 0.4766960169946799 2304 80-81 0.35130818977280853 2304 82-83 0.48658904820677407 2304 84-85 0.3668383685531964 2304 86-87 0.4455668033964173 2304 88-89 0.3948276952615828 2304 90-91 0.4448281201293085 2304 92-93 0.36273617288492943 2304 94-95 0.4692193262871953 2304 96-97 0.385242396039736 2304 98-99 0.5523179535303413 2304 100-101 0.37000088749017834 2202 1 0.07414392440614392 2202 2 0.06309849060760797 2202 3 0.037033296293955686 2202 4 0.07007048496221557 2202 5 0.11573568821787461 2202 6 0.06379927699750709 2202 7 0.10732589415987803 2202 8 0.11166871137726986 2202 9 0.1946117520051942 2202 10-11 0.13813669994834754 2202 12-13 0.15313318099037332 2202 14-15 0.13854758500040987 2202 16-17 0.12347382567995169 2202 18-19 0.16798758987475537 2202 20-21 0.1915912066250698 2202 22-23 0.22926612580701544 2202 24-25 0.1864452324807715 2202 26-27 0.24932612958758682 2202 28-29 0.17576387898136403 2202 30-31 0.25965282749783825 2202 32-33 0.22326614696049774 2202 34-35 0.21883034312758554 2202 36-37 0.29268734939098806 2202 38-39 0.27483840654842595 2202 40-41 0.23386493012137777 2202 42-43 0.2523088400162692 2202 44-45 0.2722721654146767 2202 46-47 0.217410298887323 2202 48-49 0.26134857318619 2202 50-51 0.36510140053415796 2202 52-53 0.27155344605131404 2202 54-55 0.37467507798910304 2202 56-57 0.422878928555825 2202 58-59 0.41246760515776515 2202 60-61 0.3017107740487006 2202 62-63 0.3743993526638931 2202 64-65 0.35515126282238896 2202 66-67 0.44889376467418884 2202 68-69 0.2989700749861015 2202 70-71 0.3311873665621903 2202 72-73 0.2728079085347943 2202 74-75 0.3329278618057714 2202 76-77 0.18141718708823973 2202 78-79 0.3732603567621524 2202 80-81 0.3536845063338845 2202 82-83 0.4941988288924932 2202 84-85 0.39604222179020354 2202 86-87 0.41227761624625003 2202 88-89 0.5043415271832288 2202 90-91 0.543659728796456 2202 92-93 0.353070464915227 2202 94-95 0.4485172372502717 2202 96-97 0.5434473265753823 2202 98-99 0.5464114248369683 2202 100-101 0.35550842034449204 1102 1 0.13194302290694537 1102 2 0.044599387867783946 1102 3 0.1059810291223684 1102 4 0.09022139423295528 1102 5 0.10763067345193633 1102 6 0.0358429106892757 1102 7 0.09966597696215729 1102 8 0.12210095057128711 1102 9 0.1175250922151676 1102 10-11 -7.51403936014583E-4 1102 12-13 0.025928535019559718 1102 14-15 0.14116165008356774 1102 16-17 0.09570680246423535 1102 18-19 0.10208630262432905 1102 20-21 0.10894718576906115 1102 22-23 0.11052469216894423 1102 24-25 0.0725179176201678 1102 26-27 0.12283717224630664 1102 28-29 0.14999979482259818 1102 30-31 0.1268159310289434 1102 32-33 0.1907295147241257 1102 34-35 0.09429981508643692 1102 36-37 0.17422357185499493 1102 38-39 0.1381983986355806 1102 40-41 0.17892646662644296 1102 42-43 0.12489789485511693 1102 44-45 0.0635510790940117 1102 46-47 0.17011408998819633 1102 48-49 0.11082505366548645 1102 50-51 0.06725411845533813 1102 52-53 0.2847728438272341 1102 54-55 0.18512921880949307 1102 56-57 0.19654822812768913 1102 58-59 0.15400368632095507 1102 60-61 0.040714744601615394 1102 62-63 0.2808836470782765 1102 64-65 0.19004479265805685 1102 66-67 0.004834757453714644 1102 68-69 0.19061354476892234 1102 70-71 0.1692283720587895 1102 72-73 0.024930246780691334 1102 74-75 0.11667190950892348 1102 76-77 -0.009076094057991924 1102 78-79 0.16881856563301767 1102 80-81 0.1449959192007384 1102 82-83 0.22933115710601726 1102 84-85 0.060693703329249615 1102 86-87 0.13325230927022602 1102 88-89 0.06245713951031462 1102 90-91 0.2746152049095514 1102 92-93 0.03965253949379388 1102 94-95 0.10598498533918388 1102 96-97 0.3079970715274172 1102 98-99 0.1148045519653138 1102 100-101 0.023294660471098183 2305 1 0.056078555071039204 2305 2 0.04643646897812914 2305 3 0.04830270298608497 2305 4 0.05331938791372437 2305 5 0.08347945703710025 2305 6 0.0812187129260522 2305 7 -0.041163922279181975 2305 8 -0.02209524422710274 2305 9 -0.017101938832063013 2305 10-11 0.0037065913637732706 2305 12-13 0.0640689647174355 2305 14-15 -0.00787819550245672 2305 16-17 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1213 60-61 -0.6801119918900866 1213 62-63 -0.4405543707152404 1213 64-65 -0.6996666326032397 1213 66-67 -0.6717662843007801 1213 68-69 -0.26346322529251154 1213 70-71 -0.12369208486422423 1213 72-73 -0.06717212371042791 1213 74-75 -0.015861761707611777 1213 76-77 -2.4024328793585106 1213 78-79 -2.0449732052559426 1213 80-81 -1.448255941186666 1213 82-83 -1.0052721267939404 1213 84-85 -0.6798649281501028 1213 86-87 -0.42960356886698037 1213 88-89 -0.5777885128197369 1213 90-91 -1.894155893273311 1213 92-93 -0.9145375869469703 1213 94-95 -0.5527799922237548 1213 96-97 -0.503980138531638 1213 98-99 -2.4529564877906154 1213 100-101 -0.8798429379350416 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 4.0 10 7.0 11 10.0 12 28.0 13 44.0 14 126.0 15 264.0 16 588.0 17 962.0 18 1555.0 19 2399.0 20 3316.0 21 4402.0 22 5619.0 23 7345.0 24 9302.0 25 12039.0 26 15514.0 27 20615.0 28 29071.0 29 37461.0 30 38608.0 31 44576.0 32 60512.0 33 85891.0 34 132969.0 35 228141.0 36 438338.0 37 729431.0 38 265109.0 39 459.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.35023455839269 25.758998161121166 24.124183283522687 14.766583996963453 2 29.091607248379553 24.228313379059163 33.401270604790405 13.278808767770878 3 36.541477021383436 20.016379466531536 27.332970367052717 16.10917314503231 4 28.2028797442598 20.428820985999494 34.06509474609683 17.303204523643874 5 31.229787228172274 18.963137974053158 33.749977582877825 16.05709721489674 6 25.472902786444333 19.768456164439623 38.502474126399555 16.256166922716485 7 31.54751421685772 21.249759162958505 29.51243546831826 17.690291151865516 8 28.03784601787735 28.87230325740433 26.110276004997456 16.979574719720862 9 29.97617610096441 25.61324353119754 26.282207212281932 18.128373155556126 10-11 23.992040290568482 29.30375534124783 29.977985494567648 16.726218873616048 12-13 23.199171198694813 27.667911125498744 29.83981290353137 19.29310477227507 14-15 22.191748129748053 28.368718176747485 30.576325914249598 18.86320777925486 16-17 22.0413600544809 28.372856665288705 31.33599146919562 18.249791811034775 18-19 20.641241143336945 28.3688197637886 32.36129322397595 18.62864586889851 20-21 21.512967034179777 28.798661152973708 31.93181886111554 17.75655295173097 22-23 20.59231427498049 30.649623144635118 30.858823740393532 17.899238839990858 24-25 20.741345845670246 28.690324719605904 31.67831344636312 18.89001598836073 26-27 20.708927685430726 28.815628955992693 30.73032367118881 19.74511968738777 28-29 21.240102689695668 29.717014751872323 30.663716997278257 18.379165561153755 30-31 20.083671041662164 29.29293463441282 31.35422381037997 19.269170513545042 32-33 19.933696815249135 30.269663913345568 30.77446754896177 19.022171722443527 34-35 20.11560637823854 29.298613560355484 31.770946614877317 18.81483344652866 36-37 20.986027309892165 29.263489119106417 30.92916216510136 18.82132140590006 38-39 21.530876062357954 29.92878336232197 30.296626779984848 18.24371379533522 40-41 21.449350934805164 29.970140770849557 29.903442053851453 18.677066240493822 42-43 20.190887942542325 29.94968452815335 31.235621441141426 18.623806088162898 44-45 19.945849952499152 29.208745306244822 31.50067273708809 19.344732004167938 46-47 21.045301663127283 29.049429360547197 30.4757520769547 19.42951689937082 48-49 20.336524787553813 30.357818265762198 30.628418573541595 18.677238373142398 50-51 21.2617663735909 29.39412459652071 30.408071103409807 18.936037926478587 52-53 20.13610678850887 29.25334863718468 29.78702906628113 20.823515508025324 54-55 21.09636060379653 29.352030136015422 30.42579010801529 19.125819152172756 56-57 20.29399936214063 29.39453257486167 30.43821674387739 19.873251319120307 58-59 20.240955820550234 29.29206867171897 30.869595712559345 19.597379795171456 60-61 20.74443525712189 29.593689382449433 30.802867241758314 18.859008118670367 62-63 19.887146179290465 29.605906051872932 30.433375042479412 20.07357272635719 64-65 20.68995410270325 29.400966694566826 30.412615778000568 19.496463424729356 66-67 20.00509195181827 30.57654969896767 29.80806515490435 19.61029319430971 68-69 19.87190765686045 31.089313900758448 29.62664994157026 19.41212850081084 70-71 19.569931944531653 31.80418406923814 29.361579492232465 19.264304493997734 72-73 19.626057314021057 31.41749190325953 29.44955044173866 19.506900340980753 74-75 19.91848489824757 30.852943922890695 29.61443968659856 19.614131492263173 76-77 20.04476200377011 30.56567286523744 29.750540722797574 19.63902440819488 78-79 19.934986660215618 30.57135485390165 29.862674496843383 19.630983989039347 80-81 19.672434192767838 30.95718523512092 29.70224592772988 19.668134644381364 82-83 19.453241287217082 30.661670744610653 29.711496767150702 20.173591201021562 84-85 19.55744773214726 30.334921694791795 30.080526339024576 20.02710423403637 86-87 19.71657012176892 30.374777265999363 30.2637667320725 19.64488588015922 88-89 20.004562814944045 30.825126667560525 29.568903680218426 19.601406837277004 90-91 20.176304031659388 30.416573526476192 29.419122578010082 19.987999863854338 92-93 19.731112707815644 30.71929443650692 29.65814780843103 19.891445047246407 94-95 19.36983256052929 31.073346275625752 29.57661868542893 19.980202478416032 96-97 19.60817234492168 31.356079165911517 29.297882303587087 19.73786618557972 98-99 20.036741662685593 30.73520356845406 28.959647883582246 20.268406885278104 100-101 20.02025204900784 30.649358056502436 28.72092878849695 20.60946110599278 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 25.0 1 20.0 2 21.0 3 51.5 4 58.5 5 38.0 6 35.0 7 33.0 8 35.5 9 43.0 10 46.5 11 44.5 12 47.0 13 60.0 14 78.0 15 97.5 16 118.0 17 168.0 18 242.5 19 395.0 20 663.0 21 1052.5 22 1715.0 23 2836.0 24 4300.5 25 6431.0 26 10061.0 27 14858.5 28 20324.0 29 25923.5 30 31606.0 31 37235.5 32 43870.0 33 53428.5 34 65294.5 35 80902.0 36 100831.0 37 121748.0 38 141616.0 39 152696.0 40 154709.0 41 145586.5 42 129351.0 43 118422.0 44 107002.0 45 97234.5 46 93603.0 47 84321.0 48 67512.0 49 54086.0 50 44386.5 51 36076.0 52 28960.5 53 23005.5 54 18679.5 55 14796.5 56 10957.0 57 7840.5 58 5600.5 59 3982.0 60 2890.0 61 2070.0 62 1408.5 63 928.5 64 642.5 65 468.0 66 333.0 67 227.0 68 159.5 69 115.0 70 86.0 71 64.5 72 46.0 73 27.5 74 22.0 75 20.0 76 15.0 77 11.5 78 7.0 79 4.0 80 3.0 81 1.0 82 1.0 83 1.0 84 1.5 85 1.5 86 0.5 87 0.5 88 1.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17560986376555554 2 0.006989447314051962 3 0.0015174457984454919 4 0.0 5 0.0013794961804049924 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.0692442706074887E-4 12-13 0.0 14-15 0.0 16-17 0.0 18-19 1.1495801503374937E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 2.2991603006749874E-5 38-39 2.0692442706074887E-4 40-41 1.1495801503374937E-4 42-43 0.001287529768377993 44-45 4.1384885412149773E-4 46-47 0.005931833575741468 48-49 0.004483362586316226 50-51 0.008460909906483955 52-53 0.004184471747228477 54-55 0.01984175339482514 56-57 0.011334860282327688 58-59 0.020669451103068136 60-61 0.009794422880875446 62-63 0.005701917545673969 64-65 0.013197180125874428 66-67 0.03163644573728783 68-69 0.03349876558083457 70-71 0.037637254122049546 72-73 0.03145251291323383 74-75 0.03467133733417881 76-77 0.035177152600327305 78-79 0.017657551109183905 80-81 0.002758992360809985 82-83 0.0 84-85 0.006391665635876465 86-87 0.027084108341951352 88-89 0.028072747271241597 90-91 0.025980511397627356 92-93 0.03609681672059731 94-95 0.03207328619441607 96-97 0.03407355565600331 98-99 0.04048821289488653 100-101 0.02786582284418085 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2174707.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.42186187580891 #Duplication Level Percentage of deduplicated Percentage of total 1 92.49897142219405 61.43953903459369 2 3.7231140525995596 4.9459233469930215 3 1.228884436369023 2.4487437688150355 4 0.6156672745212602 1.6357506667882753 5 0.3384103976936277 1.1238924346471852 6 0.26785297802690966 1.0674776105716484 7 0.15420605374197893 0.7169857241444304 8 0.13076585788326056 0.6948569400314873 9 0.12633740286840778 0.7552408970766424 >10 0.7877612443595425 9.92021960173962 >50 0.06069803212854119 2.7087511720476054 >100 0.0636785823686345 8.918185039668465 >500 0.002814290881515532 1.2022032921061288 >1k 7.682877637625724E-4 0.9170190571648582 >5k 0.0 0.0 >10k+ 6.968659995702693E-5 1.5052114136118466 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT 32519 1.4953278763529985 TruSeq Adapter, Index 6 (97% over 36bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 2458 0.11302672038118239 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 2344 0.10778463489564342 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 2256 0.10373811276645542 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 5.977816781754967E-4 0.0 0.0 2 6.897480902024962E-4 0.0 0.0 3 8.276977082429955E-4 0.0 0.0 4 9.656473262834947E-4 0.0 0.0 5 0.0011955633563509934 0.0 0.0 6 0.0012875297683779929 0.0 0.0 7 0.001471462592431992 0.0 0.0 8 0.0017013786224994907 0.0 0.0 9 0.0018853114465534897 0.0 0.0 10-11 0.0028969419788504843 0.0 0.0 12-13 0.003977547320167729 0.0 0.0 14-15 0.0051961022795254715 0.0 0.0 16-17 0.006414657238883215 0.0 2.2991603006749874E-5 18-19 0.007955094640335456 0.0 4.598320601349975E-5 20-21 0.009978355704929446 0.0 4.598320601349975E-5 22-23 0.012300507608611183 0.0 4.598320601349975E-5 24-25 0.015105483175434666 0.0 4.598320601349975E-5 26-27 0.017473618285129906 0.0 9.19664120269995E-5 28-29 0.020807400721108635 0.0 9.19664120269995E-5 30-31 0.032257219018470076 0.0 1.1495801503374938E-4 32-33 0.035889892293536556 0.0 1.3794961804049926E-4 34-35 0.039867439613704286 0.0 1.3794961804049926E-4 36-37 0.042787373195561515 0.0 1.3794961804049926E-4 38-39 0.04683389532474949 0.0 1.3794961804049926E-4 40-41 0.05035161058478223 0.0 1.3794961804049926E-4 42-43 0.0550878808041727 0.0 1.3794961804049926E-4 44-45 0.06072082354082642 0.0 1.3794961804049926E-4 46-47 0.06589393421734514 0.0 1.6094122104724914E-4 48-49 0.07127396932092461 0.0 1.83932824053999E-4 50-51 0.07798751739889558 0.0 1.83932824053999E-4 52-53 0.08479303188889353 0.0 1.83932824053999E-4 54-55 0.09265616011720199 0.0 1.83932824053999E-4 56-57 0.1004503135364902 0.0 1.83932824053999E-4 58-59 0.1090721646640214 0.0 1.83932824053999E-4 60-61 0.11792393182162011 0.0 1.83932824053999E-4 62-63 0.12872998523479254 0.0 1.83932824053999E-4 64-65 0.14070861040130922 0.0 2.2991603006749874E-4 66-67 0.15294014320090016 0.0 2.2991603006749874E-4 68-69 0.16717194546207836 0.0 2.2991603006749874E-4 70-71 0.18363393321491125 0.0 2.2991603006749874E-4 72-73 0.20147541714814915 0.0 2.2991603006749874E-4 74-75 0.2213631537489878 0.0 2.2991603006749874E-4 76-77 0.24506749644894693 0.0 2.2991603006749874E-4 78-79 0.27187570555481727 0.0 2.2991603006749874E-4 80-81 0.30089110854933565 0.0 2.2991603006749874E-4 82-83 0.3337001260399677 0.0 3.448740451012481E-4 84-85 0.37186618703117247 0.0 3.67865648107998E-4 86-87 0.4135269716794032 0.0 3.67865648107998E-4 88-89 0.4579007654824305 0.0 3.67865648107998E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 5070 0.0 68.38095 3 CGGAAGA 5175 0.0 66.90174 4 GATCGGA 5195 0.0 66.85703 1 ATCGGAA 5365 0.0 64.71543 2 GAAGAGC 5840 0.0 59.119644 6 GCGCACC 55 8.1985127E-7 51.814194 2 GGAAGAG 6745 0.0 51.399765 5 CGAGCGA 40 5.345609E-4 47.578396 1 GCCGTCT 3925 0.0 43.689056 50-51 TATGCCG 3900 0.0 43.541317 48-49 CGTATGC 3890 0.0 43.52964 46-47 TCGTATG 3960 0.0 43.478844 44-45 TTGTGTT 23575 0.0 43.350327 1 TGCCGTC 3935 0.0 43.09517 50-51 ATGCCGT 3960 0.0 43.061527 48-49 AATCTCG 3850 0.0 42.743267 40-41 TCTCGTA 3885 0.0 42.48386 42-43 CTCGTAT 3860 0.0 42.328835 44-45 CGAGGCG 45 9.549156E-4 42.29191 1 CGTCTTC 4095 0.0 42.05176 52-53 >>END_MODULE