FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n01_30245-1.34100000035771.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n01_30245-1.34100000035771.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2174707
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT325191.4953278763529985TruSeq Adapter, Index 6 (97% over 36bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC24580.11302672038118239No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC23440.10778463489564342No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG22560.10373811276645542No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG50700.068.380953
CGGAAGA51750.066.901744
GATCGGA51950.066.857031
ATCGGAA53650.064.715432
GAAGAGC58400.059.1196446
GCGCACC558.1985127E-751.8141942
GGAAGAG67450.051.3997655
CGAGCGA405.345609E-447.5783961
GCCGTCT39250.043.68905650-51
TATGCCG39000.043.54131748-49
CGTATGC38900.043.5296446-47
TCGTATG39600.043.47884444-45
TTGTGTT235750.043.3503271
TGCCGTC39350.043.0951750-51
ATGCCGT39600.043.06152748-49
AATCTCG38500.042.74326740-41
TCTCGTA38850.042.4838642-43
CTCGTAT38600.042.32883544-45
CGAGGCG459.549156E-442.291911
CGTCTTC40950.042.0517652-53
Produced by FastQC (version 0.11.2)