##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n01_30230-1.34100000035714.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2203502 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.691128485474486 33.0 33.0 33.0 33.0 33.0 2 32.71040189661729 33.0 33.0 33.0 33.0 33.0 3 32.734488555036485 33.0 33.0 33.0 33.0 33.0 4 36.68036334888736 37.0 37.0 37.0 37.0 37.0 5 36.64630529039683 37.0 37.0 37.0 37.0 37.0 6 36.5347524077582 37.0 37.0 37.0 37.0 37.0 7 36.47348085002873 37.0 37.0 37.0 37.0 37.0 8 36.39331391575774 37.0 37.0 37.0 37.0 37.0 9 36.41408811972941 37.0 37.0 37.0 37.0 37.0 10-11 36.336279476941705 37.0 37.0 37.0 37.0 37.0 12-13 36.30588331664777 37.0 37.0 37.0 37.0 37.0 14-15 38.10496314503005 40.0 37.0 40.0 35.0 40.0 16-17 38.05056405666979 40.0 37.0 40.0 37.0 40.0 18-19 38.08483609272876 40.0 37.0 40.0 37.0 40.0 20-21 38.00606239522361 40.0 37.0 40.0 33.0 40.0 22-23 37.8752569773025 40.0 37.0 40.0 33.0 40.0 24-25 37.87289460141175 40.0 37.0 40.0 33.0 40.0 26-27 37.74984297722444 40.0 37.0 40.0 33.0 40.0 28-29 37.61924087203007 40.0 37.0 40.0 33.0 40.0 30-31 37.560027628747335 40.0 37.0 40.0 33.0 40.0 32-33 37.47449627910481 40.0 37.0 40.0 33.0 40.0 34-35 37.33975508077596 40.0 37.0 40.0 33.0 40.0 36-37 37.23150557612382 40.0 37.0 40.0 33.0 40.0 38-39 37.10565862885534 38.5 37.0 40.0 33.0 40.0 40-41 37.00680734576143 37.0 37.0 40.0 33.0 40.0 42-43 36.785235502395736 37.0 37.0 40.0 33.0 40.0 44-45 36.64615257894025 37.0 37.0 40.0 33.0 40.0 46-47 36.59628219080355 37.0 37.0 40.0 33.0 40.0 48-49 36.35864092703342 37.0 37.0 40.0 33.0 40.0 50-51 36.2195924941298 37.0 37.0 40.0 33.0 40.0 52-53 36.099556977937844 37.0 37.0 40.0 33.0 40.0 54-55 35.878311433345644 37.0 37.0 40.0 33.0 40.0 56-57 35.65753083046896 37.0 33.0 40.0 27.0 40.0 58-59 35.44269916705317 37.0 33.0 40.0 27.0 40.0 60-61 35.21861155560558 37.0 33.0 40.0 27.0 40.0 62-63 34.95946384437137 37.0 33.0 37.0 27.0 40.0 64-65 34.64743031773967 37.0 33.0 37.0 27.0 40.0 66-67 34.16585666815823 37.0 33.0 37.0 27.0 40.0 68-69 33.86079999019742 37.0 33.0 37.0 27.0 38.5 70-71 33.54808187149365 37.0 33.0 37.0 27.0 37.0 72-73 33.02178759084403 37.0 33.0 37.0 27.0 37.0 74-75 32.45042663905002 35.0 33.0 37.0 22.0 37.0 76-77 29.6788080972924 33.0 27.0 33.0 22.0 37.0 78-79 32.14585033278844 33.0 33.0 37.0 22.0 37.0 80-81 32.66648090176456 35.0 33.0 37.0 27.0 37.0 82-83 32.49336919140532 35.0 33.0 37.0 22.0 37.0 84-85 32.41491748135468 33.0 33.0 37.0 22.0 37.0 86-87 32.29162419639283 33.0 33.0 37.0 22.0 37.0 88-89 32.19986571375928 33.0 33.0 37.0 22.0 37.0 90-91 32.090008994772866 33.0 33.0 37.0 22.0 37.0 92-93 31.908131011453587 33.0 33.0 37.0 22.0 37.0 94-95 31.75060517303819 33.0 33.0 37.0 22.0 37.0 96-97 31.608692435949685 33.0 33.0 37.0 18.5 37.0 98-99 31.291722675994848 33.0 33.0 37.0 15.0 37.0 100-101 29.555144946544182 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1128629296492818 2306 2 0.10655017628106123 2306 3 0.12724834941759156 2306 4 0.18559904811343841 2306 5 0.19990572970279175 2306 6 0.22463168051636728 2306 7 0.23873098803051107 2306 8 0.2614678909845338 2306 9 0.23870864013148463 2306 10-11 0.28020961358342333 2306 12-13 0.2460081102492211 2306 14-15 0.5216482244773175 2306 16-17 0.47176733125684933 2306 18-19 0.5180978208618825 2306 20-21 0.6275968061641422 2306 22-23 0.6395445704751097 2306 24-25 0.6281635446983387 2306 26-27 0.6810142018491021 2306 28-29 0.6382660209322637 2306 30-31 0.6930301090899817 2306 32-33 0.667333257054409 2306 34-35 0.6562919681218133 2306 36-37 0.5315936222037791 2306 38-39 0.6279557516470078 2306 40-41 0.7778933495551215 2306 42-43 0.8226618652994233 2306 44-45 0.9223543787822948 2306 46-47 0.8460844251473318 2306 48-49 0.848684575439485 2306 50-51 0.6697752756140858 2306 52-53 0.935084452405178 2306 54-55 0.9048010324232862 2306 56-57 0.7417688694121907 2306 58-59 0.8293422175608995 2306 60-61 0.7759088374975178 2306 62-63 0.9857792761846866 2306 64-65 0.803214063969655 2306 66-67 0.6922927167709005 2306 68-69 0.9633251429065126 2306 70-71 0.7675530800273762 2306 72-73 0.7401287968110282 2306 74-75 0.8752931634543302 2306 76-77 0.3531826246894276 2306 78-79 0.6707242687043262 2306 80-81 0.8347896213996009 2306 82-83 0.9751082722758753 2306 84-85 0.990187399589523 2306 86-87 0.9054353902757626 2306 88-89 0.9691444957676509 2306 90-91 1.0426654120865422 2306 92-93 1.1449220593956575 2306 94-95 1.1104338660413333 2306 96-97 1.22806884047489 2306 98-99 1.0607082071154252 2306 100-101 0.8299206977837663 2307 1 0.16437725136977122 2307 2 0.14884106267307118 2307 3 0.10652469620109883 2307 4 0.0916598683146006 2307 5 0.1520910876280226 2307 6 0.21523599587688125 2307 7 0.2232256056075954 2307 8 0.2510180994503699 2307 9 0.24629497089727437 2307 10-11 0.2379157827587477 2307 12-13 0.271285696638877 2307 14-15 0.5417377205082232 2307 16-17 0.45743714552203585 2307 18-19 0.43491318137822077 2307 20-21 0.46502658144503783 2307 22-23 0.4079550037778219 2307 24-25 0.4910159196777286 2307 26-27 0.5177052925847363 2307 28-29 0.4966616290165007 2307 30-31 0.514175699507625 2307 32-33 0.6302172110741466 2307 34-35 0.483126202451551 2307 36-37 0.6019968475353181 2307 38-39 0.5360692094709592 2307 40-41 0.5453026757511452 2307 42-43 0.5166915127919509 2307 44-45 0.6081399828621059 2307 46-47 0.5250363690617732 2307 48-49 0.6335805191890458 2307 50-51 0.4337668515922317 2307 52-53 0.5248977459297777 2307 54-55 0.50963314087263 2307 56-57 0.4063932039704099 2307 58-59 0.47374529272637034 2307 60-61 0.5623553931001837 2307 62-63 0.3868481788535192 2307 64-65 0.45253213336404485 2307 66-67 0.44995192224519087 2307 68-69 0.30522603919181535 2307 70-71 0.25387250974965525 2307 72-73 0.26880573071440494 2307 74-75 0.24778009017328628 2307 76-77 -0.171723963184224 2307 78-79 0.24547347665193797 2307 80-81 0.4850874507638352 2307 82-83 0.35245682538166534 2307 84-85 0.29673706922417864 2307 86-87 0.4868062592367295 2307 88-89 0.524256914206866 2307 90-91 0.6100148926886177 2307 92-93 0.696115069901392 2307 94-95 0.7376075934575397 2307 96-97 0.6735696001385136 2307 98-99 0.48874884911952066 2307 100-101 0.44952244854342993 2201 1 0.12602772016756347 2201 2 0.1480724768709436 2201 3 0.11639271828574493 2201 4 0.16617182292189625 2201 5 0.24164122104291863 2201 6 0.24134813821422796 2201 7 0.27737992392604127 2201 8 0.2347670579704655 2201 9 0.24564833052687618 2201 10-11 0.28730038678545355 2201 12-13 0.20426267465970227 2201 14-15 0.4835395927990547 2201 16-17 0.53882995351006 2201 18-19 0.5991446746758271 2201 20-21 0.5416580794886343 2201 22-23 0.5607438958014015 2201 24-25 0.6470904171307126 2201 26-27 0.5807070815571791 2201 28-29 0.5287039447404709 2201 30-31 0.6296669220731985 2201 32-33 0.6521403630405658 2201 34-35 0.7223584208301048 2201 36-37 0.6982505190815615 2201 38-39 0.7437342049359046 2201 40-41 0.6484432979496972 2201 42-43 0.7135725771053103 2201 44-45 0.804314917209318 2201 46-47 0.7784575975861969 2201 48-49 0.8884537223066289 2201 50-51 0.8768382747020098 2201 52-53 0.7924186678108782 2201 54-55 0.7622467280985461 2201 56-57 0.7660242893770359 2201 58-59 0.7073034231249338 2201 60-61 0.7494742312304723 2201 62-63 0.8267269387278091 2201 64-65 0.7568314678715709 2201 66-67 0.8769979029237618 2201 68-69 0.8887860152509575 2201 70-71 0.8614524400364161 2201 72-73 0.6993800541479658 2201 74-75 0.5373174357969646 2201 76-77 0.42982609508591807 2201 78-79 0.7576256685057174 2201 80-81 0.8179684873922923 2201 82-83 0.8504686204711547 2201 84-85 0.79862870653605 2201 86-87 0.9626076714307459 2201 88-89 1.0732203121207462 2201 90-91 1.0922207471240029 2201 92-93 1.1568118924648552 2201 94-95 1.0829277232865735 2201 96-97 1.1585093393203039 2201 98-99 1.1768936767875573 2201 100-101 0.8576756212339802 1101 1 0.11250623266337101 1101 2 0.1154676009288238 1101 3 0.1286751373612347 1101 4 0.1424387128182687 1101 5 0.15094906838658062 1101 6 0.12609413815859938 1101 7 0.04459865344873748 1101 8 0.08757811035318497 1101 9 0.1995611459278095 1101 10-11 0.24195386184452872 1101 12-13 0.2349059165627665 1101 14-15 0.4418818610030897 1101 16-17 0.41068297241968565 1101 18-19 0.411400834951408 1101 20-21 0.3733164423332127 1101 22-23 0.4251696819429185 1101 24-25 0.42975084628564275 1101 26-27 0.39958027996573975 1101 28-29 0.49474007122654484 1101 30-31 0.41092738035803933 1101 32-33 0.5046348443559978 1101 34-35 0.5249383030602885 1101 36-37 0.5507660851964644 1101 38-39 0.5886744960736152 1101 40-41 0.6350362066979756 1101 42-43 0.6330328301647654 1101 44-45 0.6499716429164195 1101 46-47 0.5484207904050393 1101 48-49 0.5829007338129486 1101 50-51 0.6961708525714627 1101 52-53 0.6227962412405645 1101 54-55 0.5725823771709244 1101 56-57 0.4680485198491482 1101 58-59 0.6977656168831672 1101 60-61 0.6122740952110917 1101 62-63 0.6342989836931636 1101 64-65 0.46921192378774634 1101 66-67 0.6084689250819437 1101 68-69 0.5967744027602677 1101 70-71 0.6459639949551459 1101 72-73 0.6033355027143656 1101 74-75 0.4633081447277405 1101 76-77 0.27573479162361636 1101 78-79 0.6365259451801606 1101 80-81 0.6235358314941308 1101 82-83 0.5413201502855145 1101 84-85 0.5712467896376765 1101 86-87 0.6248486237339392 1101 88-89 0.8261090044175532 1101 90-91 0.7011726352004999 1101 92-93 0.7400709803872765 1101 94-95 0.6197079876749996 1101 96-97 0.7261961812988602 1101 98-99 0.8814679716954146 1101 100-101 0.7654477175804466 2304 1 0.1252003731005189 2304 2 0.1236087446555203 2304 3 0.10878730079290477 2304 4 0.13864947488379187 2304 5 0.13101565489310474 2304 6 0.15497333527548562 2304 7 0.11381453421729049 2304 8 0.12615690581306183 2304 9 0.1707885711933912 2304 10-11 0.14111173837967073 2304 12-13 0.16422839761588648 2304 14-15 0.338205129017787 2304 16-17 0.32653650530812683 2304 18-19 0.35781659650177744 2304 20-21 0.43377658144503783 2304 22-23 0.40700451503331436 2304 24-25 0.5175554831438944 2304 26-27 0.42385468841178664 2304 28-29 0.46779264517648755 2304 30-31 0.3861977551080926 2304 32-33 0.42630274988635364 2304 34-35 0.46690686161129946 2304 36-37 0.31430650626648315 2304 38-39 0.38620080155558156 2304 40-41 0.3911732777811352 2304 42-43 0.49489510885081955 2304 44-45 0.545595236074135 2304 46-47 0.41988156952738365 2304 48-49 0.6154437632642029 2304 50-51 0.4673727547589621 2304 52-53 0.4900077317082179 2304 54-55 0.49631077405367563 2304 56-57 0.544704764592268 2304 58-59 0.586070384533393 2304 60-61 0.7124633637511621 2304 62-63 0.6122981732525616 2304 64-65 0.5803922749952406 2304 66-67 0.4995821981045765 2304 68-69 0.5044938356286295 2304 70-71 0.5477283191612159 2304 72-73 0.31653883408374384 2304 74-75 0.5256637314781685 2304 76-77 0.1691684825838884 2304 78-79 0.3414560978518111 2304 80-81 0.3498063435273977 2304 82-83 0.5520937067192762 2304 84-85 0.35028999462915067 2304 86-87 0.2609543047457379 2304 88-89 0.39874978394318106 2304 90-91 0.5314657114026389 2304 92-93 0.4161592261535283 2304 94-95 0.35663618026265453 2304 96-97 0.40126226093472894 2304 98-99 0.33920462711700594 2304 100-101 0.40628594076540026 2202 1 0.10698985784816983 2202 2 0.032925184126533225 2202 3 0.06767622423062392 2202 4 0.10308956674712277 2202 5 0.12385376877043086 2202 6 0.04554089740491207 2202 7 0.11526567059816273 2202 8 0.07286342754939312 2202 9 0.07709352363636413 2202 10-11 0.18068276529957217 2202 12-13 0.1490106102352442 2202 14-15 0.15091872321303157 2202 16-17 0.15878095099176903 2202 18-19 0.24401456007971944 2202 20-21 0.23218374919018459 2202 22-23 0.32222096614528795 2202 24-25 0.19938207504461047 2202 26-27 0.1639840957534986 2202 28-29 0.09897979397231182 2202 30-31 0.17402154649805368 2202 32-33 0.2549196115925696 2202 34-35 0.24068094432067966 2202 36-37 0.40667106708507816 2202 38-39 0.42667689352479243 2202 40-41 0.1698300091189182 2202 42-43 0.4833813554340196 2202 44-45 0.2767336110634986 2202 46-47 0.21750355094143003 2202 48-49 0.35194751393908774 2202 50-51 0.2801058790347781 2202 52-53 0.23719623527489375 2202 54-55 0.36194690346924574 2202 56-57 0.40339563759457064 2202 58-59 0.4055254541696911 2202 60-61 0.4203680096746538 2202 62-63 0.366283144855565 2202 64-65 0.23820650435496304 2202 66-67 0.28675558641679544 2202 68-69 0.304730920527696 2202 70-71 0.2613744233084532 2202 72-73 0.35355129196237556 2202 74-75 0.18215852401346666 2202 76-77 0.1296813579179883 2202 78-79 0.32635523397981814 2202 80-81 0.337089819820072 2202 82-83 0.3488156340915225 2202 84-85 0.48525826981209974 2202 86-87 0.3412801127449967 2202 88-89 0.44257554170388147 2202 90-91 0.44213884903599876 2202 92-93 0.38556047242963487 2202 94-95 0.5097775808726581 2202 96-97 0.4840536131178439 2202 98-99 0.5622648725330279 2202 100-101 0.19377399465197698 1102 1 0.029082423113948153 1102 2 0.033534695990447005 1102 3 0.07909468319495971 1102 4 0.09958999647920308 1102 5 0.13865480413046072 1102 6 0.1734752454952826 1102 7 0.15064190120808973 1102 8 0.16183238271310074 1102 9 0.0868337996227595 1102 10-11 0.1535294644878391 1102 12-13 0.13905004741609162 1102 14-15 0.21279108742567843 1102 16-17 0.08979924325770838 1102 18-19 0.2162583000140188 1102 20-21 0.22769711758673594 1102 22-23 0.17655013257466834 1102 24-25 0.10010443476573982 1102 26-27 0.19189785673477644 1102 28-29 0.22650466350985 1102 30-31 0.24038221025123363 1102 32-33 0.249936292154743 1102 34-35 0.23161248956192537 1102 36-37 0.23681848174686593 1102 38-39 0.0973604689433003 1102 40-41 0.31139593040039415 1102 42-43 0.18964584116970684 1102 44-45 0.3048518134752882 1102 46-47 0.22058518383276748 1102 48-49 0.1350216935344477 1102 50-51 0.2411260703662208 1102 52-53 0.2594046983399778 1102 54-55 0.10059760432449139 1102 56-57 0.3031532148540137 1102 58-59 0.1399994023030402 1102 60-61 0.05531365041387204 1102 62-63 0.23042275976701632 1102 64-65 0.19963736562286982 1102 66-67 0.12918676197170242 1102 68-69 0.36263168827913006 1102 70-71 0.2538389218634265 1102 72-73 0.13199027441591227 1102 74-75 0.22528410942958743 1102 76-77 0.12322608400817003 1102 78-79 0.24314137656794088 1102 80-81 0.1801131130379261 1102 82-83 0.30924346948329173 1102 84-85 0.18444105212653028 1102 86-87 0.23162695072945638 1102 88-89 0.18053655057706663 1102 90-91 0.3069725640489338 1102 92-93 0.3083997969532426 1102 94-95 0.2755000918995094 1102 96-97 0.19298763214805348 1102 98-99 0.2121067401841259 1102 100-101 0.3666979669823327 2305 1 0.05774100969574647 2305 2 0.03849349338638319 2305 3 0.08887003879836897 2305 4 0.03659913262841741 2305 5 0.06252095226930976 2305 6 0.05481470366837016 2305 7 0.08308133156406683 2305 8 0.0806120661721863 2305 9 0.010321078241275927 2305 10-11 0.027599717162495097 2305 12-13 0.07270971166635576 2305 14-15 0.0700933189497519 2305 16-17 0.06003188697746964 2305 18-19 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70-71 -0.028480606076740855 1213 72-73 0.15969109345604693 1213 74-75 0.1752903828758221 1213 76-77 -2.093259806454988 1213 78-79 -1.9278125828264834 1213 80-81 -1.4398211177587612 1213 82-83 -0.9788435342538868 1213 84-85 -0.521784199823415 1213 86-87 -0.40581390349759516 1213 88-89 -0.40834643914343616 1213 90-91 -1.7322745636861612 1213 92-93 -0.8282324625906625 1213 94-95 -0.44158934735638056 1213 96-97 -0.4117102365181253 1213 98-99 -2.294247750938947 1213 100-101 -0.7697473253216351 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 6.0 10 14.0 11 13.0 12 25.0 13 89.0 14 256.0 15 730.0 16 1473.0 17 2393.0 18 3249.0 19 3736.0 20 5004.0 21 6222.0 22 7895.0 23 10080.0 24 12577.0 25 16078.0 26 20591.0 27 27465.0 28 38835.0 29 56487.0 30 60049.0 31 59424.0 32 78180.0 33 112290.0 34 173373.0 35 297423.0 36 512038.0 37 594697.0 38 102750.0 39 59.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.53967219196055 21.886555542523485 23.70762039585362 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27.877205410843924 21.125790276473666 20-21 25.06564550429271 27.184772239825516 28.423845315320794 19.32573694056098 22-23 24.300431767250494 28.34388169377654 27.235713877273536 20.11997266169942 24-25 25.13857486855015 24.124348423554867 29.395412393544458 21.34166431435052 26-27 22.550217789682062 24.169095376359994 29.473833016716117 23.806853817241826 28-29 23.155277372110394 27.391965153650872 27.02255319033067 22.43020428390807 30-31 20.202704603853324 27.03834623249718 29.048510053542042 23.710439110107455 32-33 20.949356978119376 26.52411933367884 28.19078448760201 24.335739200599775 34-35 21.81899539914191 26.745063085942288 28.891578042588574 22.54436347232723 36-37 20.342482103487995 29.428042270894238 29.54034078480528 20.68913484081249 38-39 20.197653867247926 27.526975852264023 27.240930729020235 25.034439551467813 40-41 23.213790965783762 26.62438696973293 25.371312600110596 24.79050946437271 42-43 23.244122568438613 28.42696817884147 26.953951288671373 21.37495796404854 44-45 20.758760311487826 28.457369199483185 26.667957044487174 24.115913444541814 46-47 22.473030194382293 28.0980897616854 26.63377795124785 22.795102092684456 48-49 21.377446312955602 27.67137238599304 28.950430649109705 22.000750651941665 50-51 22.087081430918516 26.687846632839236 26.75479045449182 24.470281481750433 52-53 20.44254597250307 27.877092297187613 26.221204002251962 25.459157728057356 54-55 22.322527083333807 27.757758918668408 28.602492486024563 21.317221511973226 56-57 21.976128419526177 27.17089205820045 26.52913483392129 24.323844688352082 58-59 20.508622982697748 26.420928832817538 27.336766125027573 25.733682059457134 60-61 20.903132456189322 26.20575531013436 29.767569998824484 23.123542234851836 62-63 20.30521854684547 26.168912920741484 29.629200153219703 23.896668379193343 64-65 23.209716899818584 28.063582595055493 27.578314035002517 21.148386470123405 66-67 21.866498759421802 30.1760915124749 26.02651575594464 21.93089397215866 68-69 21.270330409660037 27.749345084622036 28.33111377493428 22.649210730783643 70-71 20.9142757264187 30.971717311583998 26.69239299078733 21.421613971209975 72-73 23.326165922422632 30.434341469438024 25.977003301942148 20.262489306197196 74-75 24.69933353202603 28.266790573431216 26.394615049541898 20.639260845000855 76-77 23.33615029164939 27.233086389823903 26.43142227340113 22.999341045125586 78-79 23.341205498926296 28.82853461067277 26.222186616168607 21.60807327423233 80-81 22.59709151491786 29.17795483853839 26.3779814332028 21.846972213340944 82-83 22.079036007228495 28.80861918890929 27.00698705968953 22.105357744172686 84-85 22.685808491735983 28.28748137802079 26.55584437982143 22.470865750421794 86-87 20.328542327486907 29.114638007637616 27.878365572849578 22.6784540920259 88-89 19.791128072737475 29.367292195992427 28.377951419073476 22.46362831219662 90-91 21.255752576346186 28.620956854656033 26.844228993620302 23.279061575377476 92-93 21.69335277002343 28.681277173950054 25.830625680129042 23.794744375897476 94-95 22.2953392420642 29.75201171425906 25.748421714234095 22.204227329442652 96-97 21.286032652686927 31.231242273893784 24.450518645026534 23.032206428392758 98-99 21.35655634623225 29.03091508543939 26.355866257092707 23.25666231123565 100-101 22.809530975397674 29.770078235454466 25.926919564946505 21.493471224201354 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 253.0 1 171.5 2 96.5 3 159.0 4 185.5 5 149.0 6 151.5 7 171.5 8 177.5 9 184.5 10 197.5 11 198.5 12 208.0 13 218.5 14 234.5 15 247.0 16 271.5 17 300.0 18 305.0 19 339.0 20 382.5 21 414.5 22 450.0 23 527.0 24 630.0 25 765.0 26 1046.5 27 1372.0 28 1743.0 29 2308.0 30 3044.5 31 4027.0 32 5545.0 33 8251.5 34 13097.0 35 22775.0 36 35241.0 37 49543.5 38 67008.5 39 81493.5 40 92134.5 41 99561.0 42 132838.5 43 169634.0 44 160620.5 45 137116.5 46 131930.0 47 128202.0 48 113557.5 49 102339.0 50 92943.0 51 87668.5 52 86518.0 53 86809.5 54 73962.0 55 47414.5 56 31144.5 57 24812.5 58 21486.0 59 18065.5 60 14284.5 61 11262.5 62 8671.0 63 6568.0 64 4974.0 65 3762.0 66 2886.0 67 2258.0 68 1794.5 69 1342.0 70 954.5 71 756.5 72 527.5 73 287.5 74 192.5 75 140.0 76 90.0 77 66.0 78 55.5 79 37.5 80 20.5 81 14.5 82 11.5 83 9.0 84 6.0 85 5.0 86 3.5 87 2.0 88 2.0 89 1.5 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17245275928953094 2 0.007669609557876508 3 0.0018606745081238864 4 0.0 5 6.353522710666929E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.2691152538096177E-4 12-13 0.0 14-15 0.0 16-17 0.0 18-19 4.538230507619235E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 1.5883806776667323E-4 40-41 6.807345761428853E-5 42-43 0.001406851457361963 44-45 5.899699659905006E-4 46-47 0.005082818168533543 48-49 0.0038121136264001577 50-51 0.007351933422343162 52-53 0.004197863219547793 54-55 0.01999090538606273 56-57 0.011549796641890953 58-59 0.019786685013219864 60-61 0.009757195591381355 62-63 0.005899699659905006 64-65 0.013115486167019588 66-67 0.031699540095720354 68-69 0.033424067688615666 70-71 0.03764462206070156 72-73 0.03210798084140609 74-75 0.034967066061206205 76-77 0.035012448366282395 78-79 0.017290658234029286 80-81 0.002382571016500098 82-83 0.0 84-85 0.005650096981985948 86-87 0.02675286884241539 88-89 0.02818241145231545 90-91 0.026140207723886796 92-93 0.03607893253557292 94-95 0.03251642158709182 96-97 0.03371905267161092 98-99 0.04029948690765881 100-101 0.02725207419825351 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2203502.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.92396337054237 #Duplication Level Percentage of deduplicated Percentage of total 1 86.7195309188491 37.22345968671295 2 6.063453702684756 5.205349292660399 3 1.9341349072518876 2.490622077377022 4 1.166662142768325 2.0031105232794424 5 0.719547979155418 1.5442925550307474 6 0.440704242816546 1.1350063665540018 7 0.3544961734085307 1.0651466533669645 8 0.2625835278157852 0.9016900583738043 9 0.2366156677032881 0.9140834028053126 >10 1.703142932172739 14.235023855546688 >50 0.22359297769078543 6.707220347965751 >100 0.1605424246518231 13.6282125277108 >500 0.010100452465293884 2.994486551987776 >1k 0.004149237531491183 3.2084524130750443 >5k 2.122037240583306E-4 0.6702126185642121 >10k+ 5.305093101420664E-4 6.07363106898907 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT 58326 2.646968325873995 TruSeq Adapter, Index 27 (97% over 39bp) TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 29929 1.3582470086253609 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 23893 1.0843194151854638 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 10747 0.48772363265383917 No Hit TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 10465 0.47492582262235294 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 9139 0.4147488860913219 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 5577 0.25309711540992474 No Hit TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 4866 0.22083029650075198 No Hit TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 4482 0.20340349135149413 No Hit GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC 2665 0.12094384302805262 No Hit AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC 2624 0.11908316851992873 No Hit TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 2569 0.11658714174073816 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2546 0.11554334872398574 No Hit GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC 2405 0.1091444437082426 No Hit TGTGTTTGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAA 2338 0.10610382926813772 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0030859967451810797 0.0 0.0 2 0.003358290575638234 0.0 0.0 3 0.0037667313213239654 0.0 0.0 4 0.003993642846704927 0.0 0.0 5 0.0044020835923906584 0.0 0.0 6 0.004946671253304966 0.0 0.0 7 0.005536641219295467 0.0 0.0 8 0.005945081964981198 0.0 0.0 9 0.006761963456352661 0.0 0.0 10-11 0.010937135523362358 0.0 0.0 12-13 0.014908087217529186 0.0 0.0 14-15 0.019015185826924595 0.0 0.0 16-17 0.025300635079977235 0.0 0.0 18-19 0.03240296582440134 0.0 0.0 20-21 0.03759923975562536 0.0 0.0 22-23 0.041592882602330294 0.0 0.0 24-25 0.04576805466933999 0.0 0.0 26-27 0.050260902871883034 0.0 0.0 28-29 0.05602445561655946 0.0 0.0 30-31 0.10909906140316641 0.0 0.0 32-33 0.11722249401180485 0.0 0.0 34-35 0.12468788319683849 0.0 0.0 36-37 0.131654067026034 0.0 0.0 38-39 0.1398909553973629 0.0 0.0 40-41 0.1493758571582871 0.0 0.0 42-43 0.16001800769865424 0.0 0.0 44-45 0.17218046545907378 0.0 0.0 46-47 0.1856590100667029 0.0 0.0 48-49 0.200544406131694 0.0 0.0 50-51 0.21672319789135658 0.0 0.0 52-53 0.23773520514163363 0.0 0.0 54-55 0.2621735764251632 0.0 0.0 56-57 0.28844993106427863 0.0 0.0 58-59 0.31660965136405594 0.0 0.0 60-61 0.3500337190526716 0.0 0.0 62-63 0.3891986483334256 0.0 0.0 64-65 0.4308822955459083 0.0 0.0 66-67 0.478465642418296 0.0 0.0 68-69 0.5299518675272361 0.0 0.0 70-71 0.587700850736691 0.0 0.0 72-73 0.6509864751654413 0.0 0.0 74-75 0.7189237858645012 0.0 0.0 76-77 0.7989781720189044 0.0 0.0 78-79 0.8866794765786461 0.0 0.0 80-81 0.9885173691696219 0.0 0.0 82-83 1.1002939865722836 0.0 0.0 84-85 1.2263206477688697 0.0 0.0 86-87 1.3669604111999898 0.0 0.0 88-89 1.5223494237808723 0.0 4.538230507619235E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAAGAGC 11280 0.0 56.419804 6 TCGGAAG 11345 0.0 56.13841 3 CGGAAGA 11340 0.0 55.95375 4 CGAGTCG 35 2.7657216E-4 54.375965 1 GATCGGA 11795 0.0 54.133934 1 ATCGGAA 11845 0.0 53.855003 2 GGAAGAG 13375 0.0 47.79551 5 GCCGTCT 7300 0.0 43.75969 50-51 TTGTGTT 23315 0.0 43.405735 1 ATGCCGT 7375 0.0 43.185368 48-49 TCGTATG 7400 0.0 42.972847 44-45 TCTCGTA 7115 0.0 42.958477 42-43 AACGGTG 100 6.2755134E-10 42.82107 1 TATGCCG 7340 0.0 42.71179 48-49 TGCCGTC 7385 0.0 42.51633 50-51 CGTATGC 7375 0.0 42.443237 46-47 GTATGCC 7495 0.0 42.429123 46-47 CTCGTAT 7130 0.0 42.301857 44-45 TGTGTTT 43025 0.0 42.16586 1 TCGATCT 7335 0.0 41.73382 38-39 >>END_MODULE