FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n01_30230-1.34100000035714.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n01_30230-1.34100000035714.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2203502
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT583262.646968325873995TruSeq Adapter, Index 27 (97% over 39bp)
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA299291.3582470086253609No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC238931.0843194151854638No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC107470.48772363265383917No Hit
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT104650.47492582262235294No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT91390.4147488860913219No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC55770.25309711540992474No Hit
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT48660.22083029650075198No Hit
TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT44820.20340349135149413No Hit
GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC26650.12094384302805262No Hit
AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC26240.11908316851992873No Hit
TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT25690.11658714174073816No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25460.11554334872398574No Hit
GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC24050.1091444437082426No Hit
TGTGTTTGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAA23380.10610382926813772No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGAGC112800.056.4198046
TCGGAAG113450.056.138413
CGGAAGA113400.055.953754
CGAGTCG352.7657216E-454.3759651
GATCGGA117950.054.1339341
ATCGGAA118450.053.8550032
GGAAGAG133750.047.795515
GCCGTCT73000.043.7596950-51
TTGTGTT233150.043.4057351
ATGCCGT73750.043.18536848-49
TCGTATG74000.042.97284744-45
TCTCGTA71150.042.95847742-43
AACGGTG1006.2755134E-1042.821071
TATGCCG73400.042.7117948-49
TGCCGTC73850.042.5163350-51
CGTATGC73750.042.44323746-47
GTATGCC74950.042.42912346-47
CTCGTAT71300.042.30185744-45
TGTGTTT430250.042.165861
TCGATCT73350.041.7338238-39
Produced by FastQC (version 0.11.2)