##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n02_53115004-1.3420000003588a.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4753638 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.402840729563337 33.0 33.0 33.0 22.0 33.0 2 29.69123016939868 33.0 33.0 33.0 15.0 33.0 3 29.41627191637226 33.0 33.0 33.0 15.0 33.0 4 32.641965374729836 37.0 37.0 37.0 15.0 37.0 5 32.81292664691758 37.0 37.0 37.0 15.0 37.0 6 33.64392219180341 37.0 37.0 37.0 22.0 37.0 7 32.68986069195845 37.0 37.0 37.0 15.0 37.0 8 33.2416721677166 37.0 37.0 37.0 22.0 37.0 9 32.36299293299153 37.0 37.0 37.0 15.0 37.0 10-11 32.44810984765773 37.0 33.0 37.0 15.0 37.0 12-13 32.431396963757024 37.0 33.0 37.0 10.5 37.0 14-15 33.83353160253263 37.0 33.0 40.0 15.0 40.0 16-17 33.70966068514262 37.0 33.0 40.0 10.5 40.0 18-19 33.753040303026864 37.0 33.0 40.0 10.5 40.0 20-21 33.745927645310815 37.0 33.0 40.0 10.5 40.0 22-23 34.90019801676105 37.0 33.0 40.0 22.0 40.0 24-25 34.010651000349625 37.0 33.0 40.0 15.0 40.0 26-27 33.90058330062155 37.0 33.0 40.0 10.5 40.0 28-29 33.50067926922496 37.0 33.0 40.0 15.0 40.0 30-31 33.48522279147045 37.0 33.0 40.0 10.5 40.0 32-33 33.201716874528515 37.0 33.0 40.0 6.0 40.0 34-35 33.415856760653625 37.0 33.0 40.0 10.5 40.0 36-37 33.20683621680911 37.0 33.0 40.0 6.0 40.0 38-39 33.502356510950136 37.0 33.0 40.0 15.0 40.0 40-41 32.984539735671916 37.0 33.0 40.0 6.0 40.0 42-43 32.833959906076146 37.0 33.0 40.0 6.0 40.0 44-45 32.68472672929659 37.0 33.0 40.0 6.0 40.0 46-47 32.66336203976827 37.0 33.0 40.0 6.0 40.0 48-49 32.590033675260926 37.0 33.0 40.0 6.0 40.0 50-51 31.705280881716277 37.0 30.0 38.5 10.5 38.5 52-53 32.12326927292318 37.0 33.0 38.5 10.5 40.0 54-55 32.720397514493115 37.0 33.0 40.0 10.5 40.0 56-57 33.06433167607631 37.0 33.0 40.0 10.5 40.0 58-59 32.38017156544104 37.0 33.0 40.0 6.0 40.0 60-61 32.2469055279346 37.0 33.0 40.0 6.0 40.0 62-63 31.81150762847318 37.0 33.0 40.0 6.0 40.0 64-65 31.65039418230837 37.0 33.0 37.0 6.0 40.0 66-67 31.620252741163714 37.0 33.0 37.0 6.0 40.0 68-69 31.880024414984902 37.0 33.0 37.0 15.0 40.0 70-71 31.374414395879533 37.0 33.0 37.0 10.5 40.0 72-73 31.92374419339462 37.0 33.0 37.0 15.0 37.0 74-75 32.13315885643796 37.0 30.0 37.0 22.0 37.0 76-77 31.755297731968653 37.0 27.0 37.0 22.0 37.0 78-79 31.104953721760047 35.0 27.0 37.0 15.0 37.0 80-81 30.487533442807383 33.0 27.0 37.0 15.0 37.0 82-83 30.096232296190834 33.0 27.0 37.0 6.0 37.0 84-85 29.867120403362648 33.0 27.0 37.0 6.0 37.0 86-87 29.705141514772478 33.0 27.0 37.0 6.0 37.0 88-89 29.563748543746915 33.0 27.0 37.0 6.0 37.0 90-91 29.41303250268531 33.0 27.0 37.0 6.0 37.0 92-93 29.26492046722952 33.0 27.0 37.0 6.0 37.0 94-95 29.147987709623663 33.0 27.0 37.0 6.0 37.0 96-97 29.014073431759 33.0 27.0 37.0 6.0 37.0 98-99 28.877183643348523 33.0 27.0 37.0 6.0 37.0 100-101 27.7632599495376 33.0 24.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -0.10510243789601503 2306 2 -0.17040138300553664 2306 3 -0.17865109881087804 2306 4 -0.06738482815180902 2306 5 -0.2067918296712037 2306 6 -0.6054787461549154 2306 7 0.22877181865353435 2306 8 -0.612388056324086 2306 9 -0.09739639073296047 2306 10-11 -0.14377183074394395 2306 12-13 -0.16787814909863386 2306 14-15 0.23140341275034615 2306 16-17 0.14932664117809225 2306 18-19 0.14933856880998775 2306 20-21 0.2780213984859685 2306 22-23 -0.013695957761804323 2306 24-25 0.16254823993057954 2306 26-27 -0.25935603354982817 2306 28-29 -0.08257958829054957 2306 30-31 -0.04725690297163254 2306 32-33 0.07184304095180494 2306 34-35 -0.23752986144015154 2306 36-37 -0.07729170610960523 2306 38-39 -0.36103856804313494 2306 40-41 -0.037922646468359744 2306 42-43 -0.15217303710046082 2306 44-45 -0.026761684984727196 2306 46-47 0.011470799658731323 2306 48-49 -0.006021389181967152 2306 50-51 -0.006692478977356586 2306 52-53 -0.14971486519978328 2306 54-55 0.1297896358392947 2306 56-57 -0.007034610732972624 2306 58-59 0.11464241712132406 2306 60-61 -0.003867498896958921 2306 62-63 0.14199625063028876 2306 64-65 0.07706687294291115 2306 66-67 -0.08404039627244586 2306 68-69 -0.20042689151712523 2306 70-71 0.0022461507340487685 2306 72-73 -0.4417458229887963 2306 74-75 -0.4548557392366419 2306 76-77 -0.0713954986513059 2306 78-79 0.1435855651254414 2306 80-81 0.2055862659402088 2306 82-83 0.15561062692507477 2306 84-85 0.21525066696116468 2306 86-87 0.32991905599146065 2306 88-89 0.3704039809551496 2306 90-91 0.31060272639285813 2306 92-93 0.3013835045715467 2306 94-95 0.21640770658214237 2306 96-97 0.05271374374808602 2306 98-99 0.16136991853667126 2306 100-101 0.0836633147336947 2307 1 -0.15793966656608305 2307 2 -0.2904495118397463 2307 3 -0.16770687306049936 2307 4 -0.07077602260876148 2307 5 -0.07946840293694635 2307 6 -0.40573982329178193 2307 7 0.24334323477427944 2307 8 -0.4710131404527118 2307 9 -0.07113712447979026 2307 10-11 -0.0648358943863343 2307 12-13 -0.12954503365237713 2307 14-15 0.22829518097660184 2307 16-17 0.17328317996926046 2307 18-19 0.2509680252059354 2307 20-21 0.25431455828435645 2307 22-23 -0.09610850031031504 2307 24-25 0.16894559817082921 2307 26-27 -0.2865531826184835 2307 28-29 -0.02101075292118537 2307 30-31 0.07852543268099055 2307 32-33 0.13692198870248262 2307 34-35 -0.07733453778099886 2307 36-37 0.00877010603299766 2307 38-39 -0.18312253688043967 2307 40-41 0.01887300934753 2307 42-43 -0.06703182206528169 2307 44-45 0.08009052463232536 2307 46-47 0.039940457672862806 2307 48-49 -0.02113529570356576 2307 50-51 -0.13710167090681935 2307 52-53 -0.19041186174748503 2307 54-55 -0.03293051484172338 2307 56-57 -0.07226971088839917 2307 58-59 0.30124100618146343 2307 60-61 0.17694894818796314 2307 62-63 0.21471770863376705 2307 64-65 0.14812367368275758 2307 66-67 4.021275377681377E-4 2307 68-69 -0.1333552938238256 2307 70-71 -0.1336398023282328 2307 72-73 -0.4518659029775627 2307 74-75 -0.3238912257784854 2307 76-77 0.023282633717514045 2307 78-79 0.037005423837776164 2307 80-81 0.17773748925509025 2307 82-83 0.05967581873833083 2307 84-85 -0.03787756200425818 2307 86-87 0.10048343193866316 2307 88-89 0.16778996549001235 2307 90-91 0.2714564079581585 2307 92-93 0.11859585731620115 2307 94-95 0.03595125736677929 2307 96-97 0.050808442313417856 2307 98-99 0.22325435123135406 2307 100-101 -0.050899918516840614 2201 1 -0.14735629079513046 2201 2 -0.12743021687001743 2201 3 -0.022809289175476977 2201 4 0.08487440459657591 2201 5 0.005179930679304334 2201 6 -0.29568833231684977 2201 7 0.43010184961046605 2201 8 -0.1965897262049907 2201 9 0.21872028512808583 2201 10-11 0.10108273855657046 2201 12-13 0.08152269678388535 2201 14-15 0.4297588543767006 2201 16-17 0.3804946041756736 2201 18-19 0.42897770098254995 2201 20-21 0.45443904196001483 2201 22-23 0.19305562009935073 2201 24-25 0.3073343833233295 2201 26-27 0.11123228108321115 2201 28-29 0.3015328163652953 2201 30-31 0.34711169420987176 2201 32-33 0.3740440109963785 2201 34-35 0.1509403730999992 2201 36-37 0.2626498944107567 2201 38-39 0.13244909781655423 2201 40-41 0.36802354971985096 2201 42-43 0.302734346666675 2201 44-45 0.33099458732683473 2201 46-47 0.40874626313883056 2201 48-49 0.32153544497430175 2201 50-51 0.2665145798712061 2201 52-53 0.29160915901865536 2201 54-55 0.45383446563697305 2201 56-57 0.43619829114860664 2201 58-59 0.5549407176056675 2201 60-61 0.3385362565113894 2201 62-63 0.5391558345886089 2201 64-65 0.45994933995333653 2201 66-67 0.4306723136974462 2201 68-69 0.29377875246147056 2201 70-71 0.33504434863651866 2201 72-73 0.16602678643566904 2201 74-75 0.25128262699127646 2201 76-77 0.3096737387930517 2201 78-79 0.5766028323139842 2201 80-81 0.49197870466843696 2201 82-83 0.4132534512851542 2201 84-85 0.43400907297565894 2201 86-87 0.39855506663565876 2201 88-89 0.5107695105587595 2201 90-91 0.5098221353389114 2201 92-93 0.555817890926793 2201 94-95 0.5269229373305642 2201 96-97 0.369320438013073 2201 98-99 0.4086941463991245 2201 100-101 0.29739026990617745 1101 1 -0.20347775499880427 1101 2 -0.3026174560604389 1101 3 -0.16226102835910083 1101 4 -0.18012511965956435 1101 5 -0.15900675121116592 1101 6 -0.35642926939632247 1101 7 0.18991202781052152 1101 8 -0.45225821429099966 1101 9 -0.08559946911623229 1101 10-11 -0.16078843000627785 1101 12-13 -0.09722616482902424 1101 14-15 0.09810744549524486 1101 16-17 0.03058992285609463 1101 18-19 0.11363768004981267 1101 20-21 0.041455652217059935 1101 22-23 -0.2222217045890389 1101 24-25 0.05083096476386828 1101 26-27 -0.15803449568418415 1101 28-29 -0.0750621149236963 1101 30-31 -0.028062631932932902 1101 32-33 -0.043787378583473924 1101 34-35 -0.20374179602014664 1101 36-37 -0.020986725633015624 1101 38-39 -0.07281245936105307 1101 40-41 0.017946241342677638 1101 42-43 0.0119136545273264 1101 44-45 -0.02264842127733857 1101 46-47 -0.14507599895525658 1101 48-49 -0.0852126418557475 1101 50-51 -0.03916546199425852 1101 52-53 0.019398887029097978 1101 54-55 0.13255257380752994 1101 56-57 0.15885865081096284 1101 58-59 0.303615091322861 1101 60-61 0.17843502501442998 1101 62-63 0.2362711277147973 1101 64-65 0.25040659776573193 1101 66-67 0.1217667284645394 1101 68-69 0.10679476464051163 1101 70-71 0.24940705575500388 1101 72-73 0.017321659276131385 1101 74-75 0.18998625895582677 1101 76-77 0.2356469748027905 1101 78-79 0.5716275709946785 1101 80-81 0.3521851095400521 1101 82-83 0.2038274362516468 1101 84-85 0.2819258245965841 1101 86-87 0.35383820219547246 1101 88-89 0.2640387204161705 1101 90-91 0.3681953971747376 1101 92-93 0.2818087974227339 1101 94-95 0.219529707845318 1101 96-97 0.23955297481526117 1101 98-99 0.22317120195241458 1101 100-101 0.13148564295732612 2304 1 -0.07530663821355432 2304 2 -0.30811488788683405 2304 3 -0.13665719653744546 2304 4 -0.07894441147161757 2304 5 -0.2056358382210064 2304 6 -0.20491769039906416 2304 7 0.1287776474601614 2304 8 -0.3716157858118265 2304 9 -0.0988476311096278 2304 10-11 -0.0635181882365714 2304 12-13 -0.07472206226795919 2304 14-15 0.05127515582184827 2304 16-17 0.00964301621675645 2304 18-19 0.12158657724585709 2304 20-21 0.14850183787201132 2304 22-23 0.08996423473453063 2304 24-25 0.015162973034158256 2304 26-27 -0.044369857094629594 2304 28-29 0.012000747567938674 2304 30-31 0.13189933421575262 2304 32-33 -0.02525254087900919 2304 34-35 -0.17514738972145238 2304 36-37 -0.0010476864320878576 2304 38-39 -0.11056431514048626 2304 40-41 0.06831339834191397 2304 42-43 -0.07099895647879606 2304 44-45 0.12836388936799636 2304 46-47 -0.05743107635592537 2304 48-49 -0.046383308396031 2304 50-51 0.017188323914261616 2304 52-53 0.005735433294724146 2304 54-55 0.21948006659472696 2304 56-57 0.35740216757962884 2304 58-59 0.3757754377745144 2304 60-61 0.14428172252682714 2304 62-63 0.22083483441573648 2304 64-65 0.19303768718082992 2304 66-67 0.04709715495316047 2304 68-69 0.06897078080193708 2304 70-71 0.174149497860018 2304 72-73 0.0053617310783558025 2304 74-75 0.2372522056255022 2304 76-77 0.3271369005716096 2304 78-79 0.2752831454420921 2304 80-81 0.2584181883269956 2304 82-83 0.09266787772868668 2304 84-85 0.10548918768952831 2304 86-87 0.25118057202431743 2304 88-89 0.25042710409597646 2304 90-91 0.20814041989433818 2304 92-93 0.15334887218709525 2304 94-95 0.02289869544580725 2304 96-97 0.0655149291153947 2304 98-99 0.1547889143525154 2304 100-101 0.12634781467838607 2202 1 -0.2733635287676002 2202 2 -0.32440551393580463 2202 3 -0.2801925399998062 2202 4 -0.19906504672311343 2202 5 -0.42318790228047476 2202 6 -0.40579187671421835 2202 7 0.028409626091487894 2202 8 -0.4840374791016018 2202 9 -0.3048132910894168 2202 10-11 -0.2357421305487719 2202 12-13 -0.21880369778211417 2202 14-15 -0.18393822409454685 2202 16-17 -0.12998307560685873 2202 18-19 -0.03451100356846837 2202 20-21 0.02096575588046079 2202 22-23 -0.09254922186510584 2202 24-25 -0.25810740853307834 2202 26-27 -0.2933189673547716 2202 28-29 -0.2001464572763041 2202 30-31 -0.148865650560019 2202 32-33 -0.2492893845449231 2202 34-35 -0.4614950216535334 2202 36-37 -0.3993462059085431 2202 38-39 -0.3372300198499545 2202 40-41 -0.313731924125797 2202 42-43 -0.4211070906953651 2202 44-45 -0.27658122749128466 2202 46-47 -0.3964817396699374 2202 48-49 -0.33211458455172505 2202 50-51 -0.267596777846439 2202 52-53 -0.4343731426638726 2202 54-55 -0.14007486640728928 2202 56-57 -0.10124915829452874 2202 58-59 -0.13326909802206188 2202 60-61 -0.053491011515113485 2202 62-63 -0.012194336164661479 2202 64-65 -0.13522012060954225 2202 66-67 -0.18849015165575267 2202 68-69 -0.18314118255093348 2202 70-71 -0.13133247443614593 2202 72-73 -0.20285145679929428 2202 74-75 -0.053715168419167014 2202 76-77 -0.00610283811813872 2202 78-79 0.12726021876624216 2202 80-81 -0.028528605829418296 2202 82-83 -0.12547468565005104 2202 84-85 -0.0801412284446883 2202 86-87 -0.09882734362252776 2202 88-89 -0.21818117309159035 2202 90-91 -0.08084509969613052 2202 92-93 -0.027876324460848423 2202 94-95 -0.08278805205523909 2202 96-97 -0.04068144188125089 2202 98-99 -0.062150232640981073 2202 100-101 -0.20772895241537626 1102 1 -0.10995425844934559 1102 2 -0.2052931050909983 1102 3 -0.06909310197066887 1102 4 0.06615420994937438 1102 5 0.012796074947232228 1102 6 -0.1251962756401639 1102 7 0.2089301020793357 1102 8 -0.10799991655850505 1102 9 0.10408768172175797 1102 10-11 0.22861873397663857 1102 12-13 0.1385388696763954 1102 14-15 0.0799230879533468 1102 16-17 0.22654720185708044 1102 18-19 0.24701362484113787 1102 20-21 0.23266202750460252 1102 22-23 -0.08507612456931923 1102 24-25 -0.050016554131950386 1102 26-27 -0.07472098927603099 1102 28-29 0.1187794887568856 1102 30-31 0.14038133783374462 1102 32-33 0.08442951264228782 1102 34-35 0.02543336007581587 1102 36-37 0.03917594278630787 1102 38-39 -0.014930106419875244 1102 40-41 0.14567282694898864 1102 42-43 0.028898866489207364 1102 44-45 0.154032157099266 1102 46-47 0.053575660591242524 1102 48-49 0.1244442756598616 1102 50-51 0.0899981922942743 1102 52-53 0.11751061887267156 1102 54-55 0.26289894252261803 1102 56-57 0.43375217693650114 1102 58-59 0.27072846628177416 1102 60-61 0.24392204440318466 1102 62-63 0.3621495371791319 1102 64-65 0.2425377183703965 1102 66-67 0.2939579870908915 1102 68-69 0.11277603312556295 1102 70-71 0.21277971431563358 1102 72-73 0.20720204047889368 1102 74-75 0.26921958133505797 1102 76-77 0.2794705042145509 1102 78-79 0.15968212242419 1102 80-81 0.162601146345839 1102 82-83 0.16895625649482682 1102 84-85 0.34991267967381035 1102 86-87 0.2694993918109816 1102 88-89 0.3311369787139036 1102 90-91 0.25813493052998027 1102 92-93 0.35589818289759734 1102 94-95 0.30853858066818773 1102 96-97 0.26310210396412614 1102 98-99 0.17702726504804644 1102 100-101 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-0.2935525401745842 1213 70-71 -1.293804590675851 1213 72-73 -0.5913746659275034 1213 74-75 -0.8361769862006412 1213 76-77 -0.9221514006721243 1213 78-79 -0.8706008593654104 1213 80-81 -1.0121173129755299 1213 82-83 -1.042189033692516 1213 84-85 -1.0172043123345738 1213 86-87 -0.9686234956513502 1213 88-89 -0.9735243602091934 1213 90-91 -0.9582237750903531 1213 92-93 -1.0305585182683927 1213 94-95 -1.1233393179300002 1213 96-97 -1.0860214385486486 1213 98-99 -0.9461751455375733 1213 100-101 -0.8131732989934939 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 535.0 3 381.0 4 623.0 5 463.0 6 1272.0 7 6977.0 8 39802.0 9 134309.0 10 176699.0 11 119366.0 12 67795.0 13 43161.0 14 30372.0 15 23298.0 16 20105.0 17 18634.0 18 18079.0 19 18459.0 20 18880.0 21 19705.0 22 21022.0 23 22100.0 24 24119.0 25 27559.0 26 32314.0 27 39031.0 28 48479.0 29 57492.0 30 67469.0 31 86871.0 32 117744.0 33 167420.0 34 252416.0 35 427249.0 36 845273.0 37 1397406.0 38 360654.0 39 105.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.762090563193496 22.370735116696498 22.477196484983278 13.389977835126729 2 30.325627132917887 28.156718476651154 29.744716875797028 11.772937514633927 3 34.922557228515224 21.018886584627662 29.39774505459961 14.6608111322575 4 26.35499223967082 17.757280667731713 34.2104847345782 21.677242358019264 5 36.868949164926555 16.058553637753985 29.434540106511342 17.637957090808115 6 33.47545189700898 16.323293050023064 34.9626201495194 15.238634903448553 7 36.363858442541115 19.89435859658921 27.262216814218 16.47956614665168 8 25.96739701705742 33.87169792568585 24.053743670427632 16.107161386829098 9 34.24867291482861 27.13235897943413 22.023802254307633 16.59516585142963 10-11 27.600138733994406 31.442649335092593 25.62952655182341 15.327685379089587 12-13 30.4354902915141 23.82006260740809 25.706117882718654 20.038329218359156 14-15 25.965951995202303 24.127047666617113 28.30300938609063 21.603990952089962 16-17 28.59771364249181 25.135467303504242 28.991129431467915 17.275689622536035 18-19 28.68975845745268 25.44413937104254 29.288411640438788 16.57769053106599 20-21 26.656578119045566 28.786300348870746 27.792976686506893 16.764144845576794 22-23 31.14664081803098 25.169905432488378 26.874887731746423 16.808566017734226 24-25 24.93237193938184 31.29277505873109 26.725385958870856 17.04946704301621 26-27 22.29247184837389 33.89532682637337 26.580360786805656 17.231840538447088 28-29 24.338825376939987 31.442342809506137 26.357080843954662 17.861750969599218 30-31 28.533161281888685 27.0691321048312 26.633384411105965 17.764322202174153 32-33 23.68168898705322 26.52482802025455 29.155744806861307 20.63773818583093 34-35 19.089122653556426 26.40194042926852 36.05927486747011 18.449662049704944 36-37 21.668214730943124 27.073856953377874 29.322565737023226 21.935362578655777 38-39 19.810115315303154 26.418672254132385 26.266555271701346 27.50465715886311 40-41 22.51397725041272 25.88612930720464 28.489725057302138 23.1101683850805 42-43 25.47149738507975 26.059030306955783 30.732225140640907 17.737247167323567 44-45 21.99706641940331 29.056875352776355 31.402410986457607 17.543647241362727 46-47 24.525226544339525 30.494497293450856 27.524503032809704 17.455773129399915 48-49 19.993340889606102 29.123778561060533 30.871030412433488 20.011850136899877 50-51 19.000480049568203 26.630188103272573 32.15978741535975 22.209544431799472 52-53 27.02336565762133 26.159045285682986 26.841387929324036 19.976201127371645 54-55 29.340148191205508 26.284397951872762 26.59123317684343 17.7842206800783 56-57 29.57712236988922 26.13664757694243 26.20896049442797 18.077269558740376 58-59 26.74240398090206 26.12531311721742 26.527925542012337 20.60435735986818 60-61 21.187764085667215 26.493669851729816 25.835924876441517 26.482641186161448 62-63 24.941940223416804 26.714940519039853 26.805651671704066 21.537467585839277 64-65 20.911377810774063 28.911699995456097 30.636958905533575 19.539963288236265 66-67 19.49942906065453 28.606728754782868 28.470726047170242 23.423116137392363 68-69 19.14523380726032 26.51935705639464 35.59814126707634 18.737267869268695 70-71 18.662142148068867 33.07279081281085 30.557316191695534 17.707750847424748 72-73 18.386731823492973 38.40992169964356 25.65098846346356 17.552358013399907 74-75 19.037518590822263 38.13401332666891 25.0948873881401 17.73358069436873 76-77 19.325747786834363 37.13690251275269 25.54464623087096 17.992703469541983 78-79 19.049762315642802 36.351926778373254 26.433834258598555 18.16447664738539 80-81 18.324508294805174 36.313510833662214 26.338699070632188 19.023281800900424 82-83 17.963011770056088 35.37867865007948 26.735917428101263 19.922392151763177 84-85 18.315838740182592 34.30068328625772 27.084336302420635 20.29914167113905 86-87 18.4923563432401 33.860842092541404 27.87688692213825 19.769914642080245 88-89 18.94084541026121 33.96890117251235 27.487771214138316 19.60248220308812 90-91 19.388777734731462 33.03617317384217 27.1736413996524 20.40140769177396 92-93 19.035689123383552 33.587685101266125 26.838693925107776 20.53793185024254 94-95 18.67602812654838 33.99197739755632 26.71394836438007 20.618046111515227 96-97 19.326393185354195 34.056351797677394 26.51160000830684 20.105655008661568 98-99 19.951367805512554 32.87679538279902 26.303361686472808 20.868475125215618 100-101 20.031607980893906 33.02362278457306 25.715771975423934 21.228997259109097 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 181.0 1 220.5 2 297.5 3 436.0 4 479.5 5 415.0 6 490.5 7 719.0 8 973.5 9 1173.0 10 1382.0 11 1616.5 12 1745.0 13 1674.0 14 1573.5 15 1517.5 16 1454.0 17 1383.0 18 1359.5 19 1467.0 20 1706.5 21 2061.0 22 2540.5 23 3257.5 24 4293.5 25 5704.0 26 7645.5 27 10206.0 28 13089.5 29 16417.5 30 20494.5 31 25702.5 32 33685.0 33 48898.5 34 73584.0 35 115591.5 36 168449.0 37 229658.0 38 298441.0 39 334022.0 40 331928.5 41 301042.0 42 269414.5 43 274068.0 44 271177.0 45 254763.5 46 256107.0 47 238178.0 48 192274.5 49 154422.0 50 130303.5 51 114926.0 52 101772.0 53 87970.0 54 74360.0 55 60526.0 56 48042.5 57 37671.5 58 29589.5 59 22997.0 60 17391.0 61 13045.5 62 9728.5 63 7040.5 64 5021.5 65 3616.0 66 2614.5 67 1886.5 68 1387.0 69 1016.0 70 734.5 71 559.5 72 439.5 73 327.0 74 250.5 75 205.5 76 168.0 77 143.0 78 115.0 79 96.0 80 80.5 81 57.0 82 44.5 83 38.0 84 32.5 85 30.0 86 24.5 87 18.5 88 15.0 89 12.0 90 9.0 91 9.5 92 8.5 93 6.0 94 3.5 95 2.5 96 3.5 97 4.0 98 3.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1934097632171402 2 0.18240766335173186 3 0.15480774934902491 4 0.14853886644292225 5 0.15333519296168535 6 0.1352227493974089 7 0.10772801799379758 8 0.09470641222575216 9 0.09483263134466696 10-11 0.0744797984196525 12-13 0.09507455132258703 14-15 0.0987874970706646 16-17 0.09123538645559465 18-19 0.08716481987059174 20-21 0.08473510183148149 22-23 0.10448839394165059 24-25 0.4562715966171593 26-27 0.28686450251365375 28-29 0.12201181495099121 30-31 0.22867748869392243 32-33 0.2604741884005471 34-35 0.2439079290429772 36-37 0.24601158102489085 38-39 0.26400832373016203 40-41 0.2638189950517898 42-43 0.29069314912073657 44-45 0.26661685218773495 46-47 0.28528676352721855 48-49 0.2685206572313668 50-51 0.28404560885788943 52-53 0.2839404262587938 54-55 0.25940132588977116 56-57 0.27619898696535156 58-59 0.2651022227607571 60-61 0.24087867018902157 62-63 0.23375780823024386 64-65 0.2317488205875163 66-67 0.2409312614885694 68-69 0.24608520884425783 70-71 0.23211695968435123 72-73 0.22340784047922874 74-75 0.23229577010281388 76-77 0.23371573519060557 78-79 0.23590353325179578 80-81 0.24283506653220124 82-83 0.26824718247371804 84-85 0.2630932351180296 86-87 0.2626304316820086 88-89 0.26013760408344094 90-91 0.2519123248341586 92-93 0.22850919653536933 94-95 0.22694197580884365 96-97 0.222135131030171 98-99 0.24649542098073096 100-101 0.25483640108901856 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4753638.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.08584229143904 #Duplication Level Percentage of deduplicated Percentage of total 1 91.75911433428762 44.12314300680678 2 3.581900365137572 3.4447739212330637 3 1.3037897915578756 1.8808149089412058 4 0.7369152022305635 1.4174075278649116 5 0.4189340350196311 1.007239796923509 6 0.32315155518454225 0.9323408831302296 7 0.23857170711177694 0.8030345038363406 8 0.1665993689963167 0.6408856786728115 9 0.11808442116754835 0.5110369968004738 >10 1.0325722065655198 9.97376857452584 >50 0.15586266379934946 5.2502903981824725 >100 0.13150170836254002 13.219154892091007 >500 0.02225340072575135 7.502141707833035 >1k 0.010484699583688879 8.087606013746594 >5k 2.2045031412956255E-4 0.819739269537406 >10k+ 4.40899556370813E-5 0.3866219198742492 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT 18236 0.3836219754217717 Illumina Single End PCR Primer 1 (96% over 32bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 9765 0.2054216160338671 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 9041 0.19019117568481236 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 7534 0.15848914031737377 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 6378 0.13417092340645206 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 5947 0.12510418336440426 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 4924 0.10358382358942773 No Hit TTGTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTA 4800 0.10097529513185481 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.006058517707911288 0.0 2.1036519819136418E-5 2 0.006500284624113153 0.0 2.1036519819136418E-5 3 0.007173453258325518 0.0 2.1036519819136418E-5 4 0.007552110615069974 0.0 2.1036519819136418E-5 5 0.008456680967292839 0.0 2.1036519819136418E-5 6 0.009108813081686068 0.0 4.2073039638272836E-5 7 0.010055456473547207 0.0 4.2073039638272836E-5 8 0.011107282464504029 0.0 4.2073039638272836E-5 9 0.012117035415822576 0.0 6.310955945740925E-5 10-11 0.01738668363051625 0.0 6.310955945740925E-5 12-13 0.021856944092082737 0.0 8.414607927654567E-5 14-15 0.026579642791478862 0.0 9.466433918611387E-5 16-17 0.02961941990534408 0.0 1.0518259909568209E-4 18-19 0.03339547521287906 0.0 1.0518259909568209E-4 20-21 0.037665888736163756 0.0 1.472556387339549E-4 22-23 0.04257791611393211 0.0 1.472556387339549E-4 24-25 0.04851021470292858 0.0 1.472556387339549E-4 26-27 0.055115681926137416 0.0 1.472556387339549E-4 28-29 0.06196306912726632 0.0 1.472556387339549E-4 30-31 0.0983246936346436 0.0 1.472556387339549E-4 32-33 0.10677085634202688 0.0 1.472556387339549E-4 34-35 0.11435452173682556 0.0 1.472556387339549E-4 36-37 0.12250617316674092 0.0 1.472556387339549E-4 38-39 0.13156239494887914 0.0 1.472556387339549E-4 40-41 0.1420701365985378 1.0518259909568209E-5 1.472556387339549E-4 42-43 0.15295653560494088 2.1036519819136418E-5 1.472556387339549E-4 44-45 0.16481061452302426 2.1036519819136418E-5 1.472556387339549E-4 46-47 0.17652795606228325 2.1036519819136418E-5 1.472556387339549E-4 48-49 0.18896053927539286 2.1036519819136418E-5 1.472556387339549E-4 50-51 0.20229769284072535 2.1036519819136418E-5 1.472556387339549E-4 52-53 0.21658148979791897 2.1036519819136418E-5 1.472556387339549E-4 54-55 0.2322221422834469 2.1036519819136418E-5 1.472556387339549E-4 56-57 0.24851492688336807 2.1036519819136418E-5 1.472556387339549E-4 58-59 0.2666273704476445 2.1036519819136418E-5 1.472556387339549E-4 60-61 0.2852446904875803 2.1036519819136418E-5 1.472556387339549E-4 62-63 0.3056185599324139 2.1036519819136418E-5 1.472556387339549E-4 64-65 0.32791727094069845 2.1036519819136418E-5 1.6829215855309135E-4 66-67 0.3515728374773174 2.1036519819136418E-5 1.6829215855309135E-4 68-69 0.3775950124935891 2.1036519819136418E-5 1.8932867837222775E-4 70-71 0.4063624533462582 2.1036519819136418E-5 1.8932867837222775E-4 72-73 0.43701266272274 2.1036519819136418E-5 1.9984693828179595E-4 74-75 0.47237505253870826 4.2073039638272836E-5 2.2088345810093238E-4 76-77 0.5098516125964998 4.2073039638272836E-5 2.3140171801050059E-4 78-79 0.5516511774771238 4.2073039638272836E-5 2.3140171801050059E-4 80-81 0.5977632289206709 4.2073039638272836E-5 2.3140171801050059E-4 82-83 0.6495761772352038 4.2073039638272836E-5 2.4191997792006882E-4 84-85 0.7082470310107754 4.2073039638272836E-5 2.52438237829637E-4 86-87 0.7726818912167901 4.2073039638272836E-5 2.52438237829637E-4 88-89 0.8412399093073557 4.2073039638272836E-5 2.734747576487734E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 9695 0.0 89.453186 2 GAGCGGC 6040 0.0 89.15255 9 CGGGAGA 8635 0.0 87.88973 4 AGAGCGG 6505 0.0 86.78834 8 AAGCGGC 2680 0.0 84.19077 9 GAGAGCG 7710 0.0 83.984245 7 CGGAAAG 4005 0.0 83.13572 5 GTTCGGA 805 0.0 81.32998 2 TCGGGAG 8860 0.0 81.273796 3 ATCCGGA 1465 0.0 80.3122 2 GGATCGG 2715 0.0 78.995926 1 ATTCGGA 1130 0.0 76.831635 2 GATCGGG 11580 0.0 76.74769 1 ACCGGAA 1330 0.0 76.683044 3 CCGGAAA 2120 0.0 76.519264 4 TCGGAGA 1645 0.0 75.83512 4 TCGGGAA 3180 0.0 73.24313 3 AAAGCGG 3420 0.0 73.182076 8 CGGAGAG 3090 0.0 71.83572 5 AATCGGA 1430 0.0 71.66138 2 >>END_MODULE