FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n02_53115004-1.3420000003588a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n02_53115004-1.3420000003588a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4753638
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT182360.3836219754217717Illumina Single End PCR Primer 1 (96% over 32bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC97650.2054216160338671No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG90410.19019117568481236No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA75340.15848914031737377No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC63780.13417092340645206No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG59470.12510418336440426No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA49240.10358382358942773No Hit
TTGTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTA48000.10097529513185481No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA96950.089.4531862
GAGCGGC60400.089.152559
CGGGAGA86350.087.889734
AGAGCGG65050.086.788348
AAGCGGC26800.084.190779
GAGAGCG77100.083.9842457
CGGAAAG40050.083.135725
GTTCGGA8050.081.329982
TCGGGAG88600.081.2737963
ATCCGGA14650.080.31222
GGATCGG27150.078.9959261
ATTCGGA11300.076.8316352
GATCGGG115800.076.747691
ACCGGAA13300.076.6830443
CCGGAAA21200.076.5192644
TCGGAGA16450.075.835124
TCGGGAA31800.073.243133
AAAGCGG34200.073.1820768
CGGAGAG30900.071.835725
AATCGGA14300.071.661382
Produced by FastQC (version 0.11.2)