##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n02_52370180-2.342000000358cd.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5341839 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.001499109201905 33.0 33.0 33.0 27.0 33.0 2 30.490867470921533 33.0 33.0 33.0 22.0 33.0 3 30.336765110292543 33.0 33.0 33.0 22.0 33.0 4 33.70022964001723 37.0 37.0 37.0 22.0 37.0 5 33.787871742296986 37.0 37.0 37.0 22.0 37.0 6 34.375118568717625 37.0 37.0 37.0 27.0 37.0 7 33.821835888352304 37.0 37.0 37.0 22.0 37.0 8 34.07128855811641 37.0 37.0 37.0 27.0 37.0 9 33.53066631173272 37.0 37.0 37.0 15.0 37.0 10-11 33.55620629899179 37.0 37.0 37.0 15.0 37.0 12-13 33.556652212842806 37.0 37.0 37.0 18.5 37.0 14-15 35.17102069156334 40.0 37.0 40.0 15.0 40.0 16-17 35.09060035317425 40.0 37.0 40.0 18.5 40.0 18-19 35.145348259279245 40.0 37.0 40.0 18.5 40.0 20-21 35.06870620773108 40.0 37.0 40.0 15.0 40.0 22-23 35.93395429925911 40.0 37.0 40.0 27.0 40.0 24-25 35.25267702751805 40.0 37.0 40.0 22.0 40.0 26-27 35.11900929623675 40.0 37.0 40.0 18.5 40.0 28-29 34.88033521789032 40.0 37.0 40.0 15.0 40.0 30-31 34.84910898662427 40.0 37.0 40.0 15.0 40.0 32-33 34.64749031185702 40.0 37.0 40.0 15.0 40.0 34-35 34.90712889325193 40.0 37.0 40.0 18.5 40.0 36-37 34.559935445452396 40.0 37.0 40.0 15.0 40.0 38-39 34.52105772936998 40.0 37.0 40.0 15.0 40.0 40-41 35.04987757961257 40.0 37.0 40.0 18.5 40.0 42-43 34.76227166337286 38.5 37.0 40.0 15.0 40.0 44-45 34.263277309555754 37.0 37.0 40.0 15.0 40.0 46-47 34.14530651335617 37.0 37.0 40.0 15.0 40.0 48-49 34.06379816014672 37.0 37.0 40.0 15.0 40.0 50-51 33.1170365673694 37.0 33.0 38.5 15.0 38.5 52-53 33.52116031576392 37.0 35.0 38.5 15.0 40.0 54-55 34.23461302371712 37.0 37.0 40.0 15.0 40.0 56-57 34.55870394072154 37.0 37.0 40.0 18.5 40.0 58-59 34.06183993939166 37.0 35.0 40.0 15.0 40.0 60-61 33.83675256779547 37.0 33.0 40.0 15.0 40.0 62-63 33.52892852068361 37.0 33.0 40.0 10.5 40.0 64-65 33.34509220513759 37.0 33.0 40.0 10.5 40.0 66-67 33.24914818660764 37.0 33.0 40.0 15.0 40.0 68-69 33.34831160205315 37.0 33.0 38.5 15.0 40.0 70-71 32.940404512378606 37.0 33.0 37.0 15.0 40.0 72-73 33.17849190138452 37.0 33.0 37.0 18.5 40.0 74-75 33.288517400093866 37.0 33.0 37.0 22.0 40.0 76-77 33.02555065774165 37.0 33.0 37.0 22.0 37.0 78-79 32.58762890457761 37.0 33.0 37.0 22.0 37.0 80-81 32.103368989593285 37.0 33.0 37.0 15.0 37.0 82-83 31.727169706911795 37.0 33.0 37.0 15.0 37.0 84-85 31.480022890993155 37.0 33.0 37.0 15.0 37.0 86-87 31.30928730723633 37.0 33.0 37.0 15.0 37.0 88-89 31.165689662305432 37.0 33.0 37.0 15.0 37.0 90-91 31.004428531073287 35.0 33.0 37.0 15.0 37.0 92-93 30.863095649269848 33.0 33.0 37.0 15.0 37.0 94-95 30.76266085518489 33.0 33.0 37.0 15.0 37.0 96-97 30.646115130014216 33.0 33.0 37.0 15.0 37.0 98-99 30.52175720758338 33.0 33.0 37.0 15.0 37.0 100-101 29.417692951809293 33.0 27.0 37.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -0.0036313941135261985 2306 2 0.055315943641808474 2306 3 -0.012802845223482962 2306 4 0.1622678311297321 2306 5 0.06378731048025088 2306 6 -0.278110423923863 2306 7 0.27543714377905104 2306 8 -0.2262414577911187 2306 9 0.20110291087205923 2306 10-11 0.11176689883517099 2306 12-13 0.12252330595799066 2306 14-15 0.4669960367857726 2306 16-17 0.3901252528549719 2306 18-19 0.3844822741522478 2306 20-21 0.40104985462945564 2306 22-23 0.08916896895009785 2306 24-25 0.35958029399581193 2306 26-27 0.11149518600510078 2306 28-29 0.21043747813566682 2306 30-31 0.19977613481758993 2306 32-33 0.23732426495598702 2306 34-35 0.007527345575567779 2306 36-37 0.2726415383197889 2306 38-39 0.054408254023137204 2306 40-41 -0.2558513190614917 2306 42-43 0.14185515686713046 2306 44-45 0.3561404398688026 2306 46-47 0.29199423108178735 2306 48-49 0.22742605827301077 2306 50-51 0.06163108050247956 2306 52-53 0.07753255833527817 2306 54-55 0.27135029928616206 2306 56-57 0.14598781576971476 2306 58-59 0.3200250789184764 2306 60-61 0.4447207652383298 2306 62-63 0.46588111126906995 2306 64-65 0.3458614600854375 2306 66-67 0.232908780140896 2306 68-69 -0.024686180663344715 2306 70-71 0.18262957084358078 2306 72-73 -0.12447474585425056 2306 74-75 -0.25098666307160045 2306 76-77 0.004661846051554619 2306 78-79 0.11997036067663913 2306 80-81 0.19774874984605617 2306 82-83 0.17360057775112736 2306 84-85 0.21331091080717002 2306 86-87 0.22100771550691078 2306 88-89 0.334759401967343 2306 90-91 0.25007625279217294 2306 92-93 0.2525233613617992 2306 94-95 0.24069604719162285 2306 96-97 0.2958512821465824 2306 98-99 0.2592727138260784 2306 100-101 0.12003148485212023 2307 1 -0.14418363291949632 2307 2 -0.1590895289950076 2307 3 -0.21167722333293781 2307 4 -0.0358814226016122 2307 5 -0.09024627160930265 2307 6 -0.34319251347609736 2307 7 0.15601301442581672 2307 8 -0.4079864826667432 2307 9 -0.09795181549609566 2307 10-11 -0.05350237977179262 2307 12-13 -0.12499224130569786 2307 14-15 0.2174357134026934 2307 16-17 0.1851855762380481 2307 18-19 0.25030130400298845 2307 20-21 0.2588657750274663 2307 22-23 -0.07074583204492058 2307 24-25 0.2117699706127283 2307 26-27 -0.10905580901977174 2307 28-29 0.028153896046120508 2307 30-31 -0.015587049262016706 2307 32-33 0.060714190329122175 2307 34-35 -0.19805536586721928 2307 36-37 -0.0042794815807098985 2307 38-39 -0.11301400965844977 2307 40-41 -0.4123227120963122 2307 42-43 -0.07055964412790416 2307 44-45 0.1648064846449273 2307 46-47 0.08125169376835117 2307 48-49 -0.09598563326927945 2307 50-51 -0.24211829760697157 2307 52-53 -0.27002527748561533 2307 54-55 -0.10614099424617507 2307 56-57 -0.1634394230362517 2307 58-59 0.035197342600071124 2307 60-61 -0.07509515515968701 2307 62-63 0.011896036642205843 2307 64-65 0.06444106207548117 2307 66-67 -0.131081891500898 2307 68-69 -0.2590891657379686 2307 70-71 -0.32373299134547295 2307 72-73 -0.49444116376469793 2307 74-75 -0.541091140683541 2307 76-77 -0.3823804425056565 2307 78-79 -0.1812628482785854 2307 80-81 -0.04716667303951283 2307 82-83 -0.20229059140309857 2307 84-85 -0.14947204939183578 2307 86-87 0.04758793938750827 2307 88-89 0.027395595997191435 2307 90-91 -0.004651359148127199 2307 92-93 -0.07500711127501702 2307 94-95 -0.12150917668897065 2307 96-97 0.021271680156534245 2307 98-99 -0.1092944503530262 2307 100-101 -0.20610408728718355 2201 1 0.03001120164153548 2201 2 0.08361443107776623 2201 3 0.11624051901169707 2201 4 0.25499239809728635 2201 5 0.1591241609315759 2201 6 -0.11320886256743279 2201 7 0.38076265000008647 2201 8 -0.0018560589133471694 2201 9 0.26374257908381793 2201 10-11 0.19551455070147483 2201 12-13 0.17378901100800448 2201 14-15 0.4150441277835739 2201 16-17 0.4294654272877594 2201 18-19 0.4270677523176545 2201 20-21 0.4710923040073496 2201 22-23 0.22682580598353042 2201 24-25 0.48148743598165566 2201 26-27 0.1650889283812873 2201 28-29 0.28588002509834354 2201 30-31 0.3348854297675743 2201 32-33 0.33773561528533236 2201 34-35 0.16286636118913123 2201 36-37 0.5008188071658566 2201 38-39 0.26664035331565117 2201 40-41 0.13503078633007704 2201 42-43 0.4799700324465377 2201 44-45 0.5426238809520001 2201 46-47 0.4605924867539031 2201 48-49 0.36728474331813743 2201 50-51 0.23944667576962075 2201 52-53 0.2104239037853901 2201 54-55 0.38735438564869185 2201 56-57 0.4228315276506578 2201 58-59 0.6103246336879309 2201 60-61 0.4452228389148303 2201 62-63 0.5774878446186662 2201 64-65 0.4966825140010229 2201 66-67 0.438037988484389 2201 68-69 0.2967007429765758 2201 70-71 0.4040961175622826 2201 72-73 0.1622386598544594 2201 74-75 0.17325363944120653 2201 76-77 0.19056584702740764 2201 78-79 0.35612195252831214 2201 80-81 0.47454260200511555 2201 82-83 0.45173344552376093 2201 84-85 0.44442383469104385 2201 86-87 0.4989706027964971 2201 88-89 0.47663976913006323 2201 90-91 0.4004300585984666 2201 92-93 0.4410623037867829 2201 94-95 0.454102548777378 2201 96-97 0.3689094670697344 2201 98-99 0.5004673954557433 2201 100-101 0.3705263006608561 1101 1 -0.05837289071216745 1101 2 -0.09131784320626579 1101 3 -0.09165488603980876 1101 4 0.03732905561953004 1101 5 -0.06635044462178996 1101 6 -0.17389613820957806 1101 7 0.3558810213300774 1101 8 -0.26198011992264014 1101 9 -0.008556953073515672 1101 10-11 0.031247341012047514 1101 12-13 0.045481072397898004 1101 14-15 0.1840558440420068 1101 16-17 0.12774911906812036 1101 18-19 0.17947008594362757 1101 20-21 0.1815169748108545 1101 22-23 -0.004726439213165179 1101 24-25 0.19636062279399624 1101 26-27 -0.10423213825793454 1101 28-29 0.08028101555063927 1101 30-31 0.08450856112144578 1101 32-33 0.014096090352815338 1101 34-35 -0.1442461805015256 1101 36-37 0.14382946348985826 1101 38-39 -0.010561984072104735 1101 40-41 -0.2754221807394899 1101 42-43 0.09109466933877997 1101 44-45 0.1469950317055364 1101 46-47 0.06086214491398323 1101 48-49 0.036301908613147305 1101 50-51 0.06611265646619557 1101 52-53 0.05587921366408466 1101 54-55 0.211473315159175 1101 56-57 0.12974093368355 1101 58-59 0.34884509025634713 1101 60-61 0.2477184976646285 1101 62-63 0.24294233575886182 1101 64-65 0.15315057573169355 1101 66-67 0.033652260866517736 1101 68-69 -0.0029514867857898253 1101 70-71 0.12042123751024292 1101 72-73 -0.03375649188598828 1101 74-75 0.01579111470618244 1101 76-77 0.15513690274089953 1101 78-79 0.27680057609614295 1101 80-81 0.25591655029957394 1101 82-83 0.23166378636791407 1101 84-85 0.22239396069379325 1101 86-87 0.2564898583640556 1101 88-89 0.13025798473377748 1101 90-91 0.15804620744069808 1101 92-93 0.11436321396950788 1101 94-95 0.11221928982201135 1101 96-97 0.15733540913070954 1101 98-99 0.09819108117302022 1101 100-101 0.1099787637636851 2304 1 0.049094775730495144 2304 2 -0.06882357108955972 2304 3 0.10040556032244297 2304 4 0.143019997507551 2304 5 0.1449783850036468 2304 6 0.04485231437769954 2304 7 0.38505196180332035 2304 8 -0.04399319089337439 2304 9 0.09773202698990957 2304 10-11 0.1941800270847409 2304 12-13 0.19009587961483732 2304 14-15 0.36748108877884533 2304 16-17 0.31822079011666204 2304 18-19 0.32647729148326476 2304 20-21 0.40863997594660617 2304 22-23 0.25285809373345813 2304 24-25 0.35256491271331214 2304 26-27 0.24710133851809957 2304 28-29 0.21810125629858845 2304 30-31 0.28391651610008495 2304 32-33 0.20171919563190244 2304 34-35 0.00970910467435715 2304 36-37 0.30378256951563287 2304 38-39 0.13799685887583024 2304 40-41 -0.012082254936700565 2304 42-43 0.23073059014269148 2304 44-45 0.3797095473211485 2304 46-47 0.24429102484261733 2304 48-49 0.13954867525741577 2304 50-51 0.11008580320611827 2304 52-53 0.13870651847392423 2304 54-55 0.43021901679049535 2304 56-57 0.4081752074508245 2304 58-59 0.5427653302183018 2304 60-61 0.4252172990338181 2304 62-63 0.37374159653769823 2304 64-65 0.39925227464349433 2304 66-67 0.2543843000715711 2304 68-69 0.2270815836347495 2304 70-71 0.4004183490064932 2304 72-73 0.18857508083552688 2304 74-75 0.22462512202371698 2304 76-77 0.2390795236945351 2304 78-79 0.4256265565171944 2304 80-81 0.46695412246303647 2304 82-83 0.3759097198655148 2304 84-85 0.2790745589874106 2304 86-87 0.30579042781193877 2304 88-89 0.4191707971146563 2304 90-91 0.36947529958593606 2304 92-93 0.40891135962869996 2304 94-95 0.5067918010911079 2304 96-97 0.4236571746942417 2304 98-99 0.3196254001345693 2304 100-101 0.2952693531363515 2202 1 -0.021962778536163796 2202 2 -0.0366446953224866 2202 3 -0.02730247980272793 2202 4 0.10155745318026277 2202 5 0.03285564416160014 2202 6 -0.08690213409300185 2202 7 0.3494185073205003 2202 8 -0.0636734373275587 2202 9 -0.005963282487719823 2202 10-11 0.1957792970393939 2202 12-13 0.10189412972078316 2202 14-15 0.24472163189957996 2202 16-17 0.2440971937612133 2202 18-19 0.2690613355429292 2202 20-21 0.30945327914396614 2202 22-23 0.21042009131385697 2202 24-25 0.2228364278442072 2202 26-27 0.09013649943170066 2202 28-29 0.18638840734845274 2202 30-31 0.175938799256933 2202 32-33 0.09272933386390037 2202 34-35 -0.1384909515637105 2202 36-37 0.14988571768918035 2202 38-39 0.08393333754788301 2202 40-41 -0.10160027500218405 2202 42-43 0.143916991279319 2202 44-45 0.17641385289866918 2202 46-47 0.17009023233465825 2202 48-49 0.07986148320514985 2202 50-51 0.05528630601929052 2202 52-53 0.06806823906194381 2202 54-55 0.2875819238424313 2202 56-57 0.21188228747571003 2202 58-59 0.3401606239174271 2202 60-61 0.28310832005143993 2202 62-63 0.2692922618224216 2202 64-65 0.24699332477535307 2202 66-67 0.2656313474827172 2202 68-69 0.13067297573674352 2202 70-71 0.23657979531632378 2202 72-73 0.08393948467404755 2202 74-75 0.11085735446859957 2202 76-77 0.26763438729189915 2202 78-79 0.30220081066619997 2202 80-81 0.22975083796465867 2202 82-83 0.29136428102947676 2202 84-85 0.3166650818240839 2202 86-87 0.29978848915485656 2202 88-89 0.2901324182546645 2202 90-91 0.35341259649649714 2202 92-93 0.219242900931647 2202 94-95 0.2367830695344928 2202 96-97 0.21937821887659226 2202 98-99 0.22760159250638878 2202 100-101 0.07326509312106921 1102 1 -0.20343139411352595 1102 2 -0.33278405635819297 1102 3 -0.17075284522348255 1102 4 -0.18153216887026957 1102 5 -0.19716268951975024 1102 6 -0.22841042392386157 1102 7 0.16478714377905135 1102 8 -0.20459145779111765 1102 9 -0.06269708912793703 1102 10-11 -0.024058101164825985 1102 12-13 -0.100801694042012 1102 14-15 -0.11022896321422593 1102 16-17 -0.1205497471450272 1102 18-19 -0.06479272584775231 1102 20-21 0.041949854629450556 1102 22-23 -0.17210603104989985 1102 24-25 -0.15579470600419398 1102 26-27 -0.30945481399489694 1102 28-29 -0.18026252186432856 1102 30-31 -0.062423865182410054 1102 32-33 -0.1767007350440153 1102 34-35 -0.22909765442443586 1102 36-37 -0.08298346168021453 1102 38-39 -0.21176674597685974 1102 40-41 -0.26970131906148254 1102 42-43 -0.09161984313287519 1102 44-45 -0.07938456013119577 1102 46-47 -0.09845576891821395 1102 48-49 -0.13562394172699044 1102 50-51 -0.07939391949752661 1102 52-53 -0.11859244166471683 1102 54-55 0.07470029928615673 1102 56-57 0.10481281576971924 1102 58-59 0.16595007891847047 1102 60-61 0.07202076523832801 1102 62-63 0.16908111126907244 1102 64-65 0.10296146008543161 1102 66-67 0.0718837801408938 1102 68-69 0.023013819336661356 1102 70-71 0.11675457084357532 1102 72-73 -0.018224745854247715 1102 74-75 0.07471333692840432 1102 76-77 0.16616184605155837 1102 78-79 0.15779536067663713 1102 80-81 0.15344874984604928 1102 82-83 0.18057557775113153 1102 84-85 0.1817359108071699 1102 86-87 0.17873271550691427 1102 88-89 0.13963440196734211 1102 90-91 0.1642262527921723 1102 92-93 0.1483983613617994 1102 94-95 0.18384604719162567 1102 96-97 0.12985128214658204 1102 98-99 0.096772713826077 1102 100-101 0.11918148485212399 2305 1 -0.00843006457886375 2305 2 -0.15248225198972065 2305 3 -0.019743728414365336 2305 4 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-0.9676021447845145 1213 64-65 -0.871145495092307 1213 66-67 -0.7580415676179939 1213 68-69 -0.5294981332650934 1213 70-71 -1.500689014936949 1213 72-73 -0.7793656526033459 1213 74-75 -0.8840500324688207 1213 76-77 -0.9508201220061423 1213 78-79 -1.0156686784784341 1213 80-81 -1.231835485084389 1213 82-83 -1.2885872764477355 1213 84-85 -1.1936864488012802 1213 86-87 -1.1280597110773236 1213 88-89 -1.2697731199285904 1213 90-91 -1.2188496874448198 1213 92-93 -1.2182603197304225 1213 94-95 -1.23741227326175 1213 96-97 -1.2095379502078742 1213 98-99 -1.1789354263078735 1213 100-101 -1.0656502513663249 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 598.0 3 406.0 4 556.0 5 433.0 6 1188.0 7 6072.0 8 31196.0 9 97721.0 10 142421.0 11 107995.0 12 60380.0 13 37849.0 14 26305.0 15 20108.0 16 17055.0 17 16085.0 18 15766.0 19 15914.0 20 16707.0 21 17851.0 22 18838.0 23 19962.0 24 21903.0 25 24793.0 26 29057.0 27 34478.0 28 41969.0 29 51154.0 30 61334.0 31 79142.0 32 106426.0 33 152642.0 34 233760.0 35 401069.0 36 841404.0 37 1776423.0 38 813773.0 39 1106.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 38.5399738909271 23.838746464396376 25.09093159873056 12.530348045945965 2 29.720732123804567 26.297639050203774 33.549579158030994 10.432049667960666 3 36.61440700290369 20.17135274963678 29.177890410512003 14.036349836947522 4 25.3733215271785 18.238840662092063 39.142034004500616 17.245803806228828 5 33.306972675284314 17.41584556779095 34.03515304368107 15.242028713243663 6 28.058494710084197 17.58232262178728 40.89613559939664 13.463047068731884 7 35.79527108407205 18.732404211351668 30.710143213767083 14.762181490809196 8 25.805962013715657 32.33818409873594 28.051755904872955 13.804097982675453 9 33.88115285125791 26.85263137184728 24.228314241252573 15.037901535642245 10-11 26.762434274086356 30.316855714630154 28.20590806811759 14.7148019431659 12-13 29.545801829594204 24.358469525141764 27.61109913467013 18.4846295105939 14-15 25.701735779024343 25.23071702259706 29.623831470982402 19.443715727396196 16-17 26.657326278857884 26.441213875675412 30.602960912303878 16.29849893316283 18-19 25.776152634709387 27.127928210828838 30.868654104804556 16.227265049657223 20-21 24.47479278934267 29.157337706063082 30.020448457110138 16.34742104748411 22-23 27.513833037696944 27.09540080601871 29.037774525584876 16.352991630699474 24-25 23.7720137487935 30.74666329419099 28.976132369043494 16.505190587972017 26-27 21.657902569978777 33.0520449261686 28.56390562654726 16.726146877305368 28-29 23.132963435439496 31.07773028992912 28.569017346414338 17.22028892821704 30-31 25.534160970888582 28.147426363937612 28.895927223136024 17.42248544203779 32-33 21.840360250346325 30.25974712164512 30.770873838707768 17.12901878930079 34-35 26.1576303695625 28.291328801133282 29.010127685232824 16.540913144071396 36-37 22.966947391869844 29.73558044864423 30.823021136917838 16.474451022568086 38-39 21.96819008988125 32.274867505633416 28.915747003567283 16.841195400918053 40-41 26.684845677839203 27.830301699319904 28.16878606532337 17.316066557517516 42-43 26.359521551151175 27.969727829083784 28.874291898257148 16.796458721507893 44-45 23.31212558341647 29.870867046272565 29.96130422500049 16.85570314531047 46-47 23.6808630294137 30.455210617435096 28.84841704502242 17.015509308128788 48-49 20.625152164009197 30.51027056614491 30.37979198135795 18.484785288487945 50-51 19.16735434572645 28.54330097025406 32.30546786754471 19.983876816474787 52-53 24.32620097022517 28.00849076095155 28.76954462835836 18.895763640464917 54-55 25.78377331349281 27.92591748663993 28.48199900296857 17.80831019689869 56-57 26.27764823199336 27.66329221877716 28.158538509545476 17.900521039684 58-59 24.89585408845028 27.773056469341213 28.573534892481963 18.757554549726546 60-61 21.28968005616296 28.20922954395369 28.068803378785272 22.43228702109808 62-63 23.6615218764997 28.270685298059593 28.41227375143725 19.655519074003447 64-65 21.238919205759295 29.516090958867508 30.615801500619412 18.629188334753792 66-67 19.521450935945904 29.433675248817572 29.628711480086288 21.416162335150236 68-69 18.736486026822682 28.435778277708835 34.30530565518989 18.522430040278596 70-71 18.146613674849775 32.2771372822501 31.742972112119705 17.833276930780418 72-73 17.957964860172908 36.0760041709606 28.19524335997801 17.77078760888848 74-75 18.39158353853836 35.95445339780302 27.805338601893748 17.848624461764874 76-77 18.828558778519096 35.50342403193893 27.998897764670883 17.669119424871095 78-79 18.832708051193258 35.21052770835237 28.339279659212007 17.617484581242362 80-81 18.254688521882898 35.41712292198574 28.15894293266375 18.169245623467614 82-83 17.92025360627393 34.85898050292896 28.30579881452432 18.914967076272784 84-85 18.089103732365768 33.91809012670716 29.009631823863636 18.983174317063437 86-87 18.1253273602244 33.56997275818603 29.670471373383656 18.634228508205908 88-89 18.48240783571994 33.84535007284024 29.08456478530218 18.58767730613764 90-91 18.95394059463917 33.075307148409806 28.907666107504106 19.063086149446924 92-93 18.64323663083405 33.658100719670145 28.765199371680655 18.933463277815154 94-95 18.41928565913462 33.82524805964971 28.687669392774893 19.06779688844078 96-97 19.054986033617823 33.69907195677106 28.39759745392032 18.8483445556908 98-99 19.41526339421231 32.848497246159425 28.150195154610593 19.58604420501767 100-101 19.320909660450138 32.84949814697824 27.875177316145823 19.9544148764258 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 515.0 1 528.0 2 538.5 3 784.0 4 990.0 5 967.5 6 1091.5 7 1372.0 8 1740.5 9 2097.5 10 2378.5 11 2544.0 12 2593.0 13 2558.0 14 2435.0 15 2397.5 16 2474.0 17 2635.5 18 3054.0 19 3638.0 20 4619.0 21 6203.5 22 8639.0 23 12225.0 24 16853.0 25 23020.0 26 31493.0 27 41863.0 28 53404.0 29 65475.0 30 78489.5 31 92327.0 32 108449.5 33 128816.5 34 154518.0 35 189853.0 36 239260.0 37 298870.5 38 352729.5 39 366803.5 40 356207.0 41 322615.0 42 273324.0 43 257369.5 44 242188.5 45 224980.5 46 231211.0 47 211551.5 48 156617.5 49 114784.5 50 92867.0 51 79958.5 52 70499.5 53 60547.5 54 52232.0 55 45648.5 56 39863.5 57 34444.0 58 29587.0 59 25390.5 60 21235.5 61 17495.5 62 14500.0 63 11923.5 64 9620.5 65 7701.5 66 6095.0 67 4768.5 68 3725.5 69 2859.0 70 2198.5 71 1689.0 72 1267.5 73 967.0 74 752.5 75 552.5 76 394.0 77 292.5 78 213.0 79 159.5 80 124.0 81 87.5 82 68.0 83 52.0 84 42.5 85 41.0 86 27.5 87 26.5 88 23.0 89 13.0 90 10.5 91 8.5 92 7.0 93 5.5 94 8.5 95 6.0 96 2.0 97 2.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19392198080099382 2 0.18327021836487398 3 0.15500279959766664 4 0.14785170425390956 5 0.15225093830046169 6 0.1322765437146271 7 0.10651762436119845 8 0.09393768700254725 9 0.09330120207666312 10-11 0.07398201256159162 12-13 0.09308592041055523 14-15 0.09762555554369946 16-17 0.0898847756362556 18-19 0.08610330637070866 20-21 0.08455889441819568 22-23 0.10502937284332231 24-25 0.45455319787810905 26-27 0.28599701338808603 28-29 0.12491952677720164 30-31 0.23312196417750516 32-33 0.2639446827206885 34-35 0.24762071638624825 36-37 0.24932424957023225 38-39 0.2668369451044855 40-41 0.26707094691547234 42-43 0.2925397040232774 44-45 0.26927056393874843 46-47 0.2881498300491647 48-49 0.2719662648013166 50-51 0.2873074235296122 52-53 0.2872325429500964 54-55 0.2625125916374492 56-57 0.278808477754571 58-59 0.2684000772018775 60-61 0.24448509211902492 62-63 0.23702511438476523 64-65 0.2353683815629786 66-67 0.24496245581343803 68-69 0.24947401072926384 70-71 0.23565854380860224 72-73 0.22710343759892426 74-75 0.23617334779277324 76-77 0.23715615539891788 78-79 0.23920601126316238 80-81 0.24587038284006688 82-83 0.27192882451155864 84-85 0.2665655030037409 86-87 0.2655171748905199 88-89 0.2636451604026254 90-91 0.25521173513466056 92-93 0.23182091410841846 94-95 0.2307164255605607 96-97 0.22607382963058228 98-99 0.25031641724881637 100-101 0.25805719715626024 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5341839.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.52198044707596 #Duplication Level Percentage of deduplicated Percentage of total 1 94.18092943275165 58.88378228481941 2 3.1468219541445333 3.934910813749078 3 0.7668153981879827 1.4382845199607748 4 0.4019967849763097 1.0053454052030495 5 0.28162998055309413 0.8804032068725469 6 0.23183482445833029 0.8696863417041009 7 0.12037441531451236 0.5268232788435501 8 0.07791504006265038 0.3897122089064134 9 0.07543092847066926 0.4244481931453173 >10 0.5542988942396136 6.963970411137378 >50 0.06830718680726715 2.9971506261696903 >100 0.07518640752584334 9.682717527265568 >500 0.012687693500160566 5.566770158467649 >1k 0.005560433967364044 5.400225820842537 >5k 2.1062503993163489E-4 1.0357692029129497 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT 9749 0.18250269242483722 Illumina Single End PCR Primer 1 (97% over 34bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 9432 0.17656840649821157 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 8292 0.155227441336214 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 8194 0.15339286713807737 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 7060 0.13216422284535342 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 6800 0.1272969851768277 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 5531 0.10354112132544616 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0016848130391050723 0.0 0.0 2 0.0017971339083787438 0.0 0.0 3 0.0019281749225313605 0.0 0.0 4 0.0020030555020471414 0.0 1.8720144878945247E-5 5 0.0023025778201102654 0.0 1.8720144878945247E-5 6 0.0023774583996260465 0.0 1.8720144878945247E-5 7 0.0026957008625681156 0.0 1.8720144878945247E-5 8 0.003051383615268075 0.0 1.8720144878945247E-5 9 0.003332185788452254 0.0 3.744028975789049E-5 10-11 0.00628060860688613 0.0 3.744028975789049E-5 12-13 0.008732947586027957 0.0 3.744028975789049E-5 14-15 0.01084832395734877 0.0 3.744028975789049E-5 16-17 0.011831131563493396 0.0 3.744028975789049E-5 18-19 0.01287945967671433 0.0 3.744028975789049E-5 20-21 0.01391842771749579 0.0 4.680036219736312E-5 22-23 0.015621960901479808 0.0 5.616043463683574E-5 24-25 0.01755013582401117 0.0 5.616043463683574E-5 26-27 0.019609351760695146 0.0 9.360072439472624E-5 28-29 0.022239532116186955 0.0 1.1232086927367147E-4 30-31 0.039621186636287616 0.0 1.3104101415261672E-4 32-33 0.042401128150810985 0.0 1.5912123147103458E-4 34-35 0.04465690560872389 0.0 1.6848130391050722E-4 36-37 0.04723092552957886 0.0 1.8720144878945247E-4 38-39 0.04974878501579699 0.0 1.9656152122892508E-4 40-41 0.05300609022473347 0.0 2.0592159366839772E-4 42-43 0.05622595514391205 0.0 2.0592159366839772E-4 44-45 0.05961430136700114 0.0 2.2464173854734297E-4 46-47 0.06349873142938228 0.0 2.4336188342628822E-4 48-49 0.06815068743180017 0.0 2.4336188342628822E-4 50-51 0.0734765686498601 0.0 2.4336188342628822E-4 52-53 0.07919557291037788 0.0 2.4336188342628822E-4 54-55 0.08583186426996395 9.360072439472623E-6 2.6208202830523344E-4 56-57 0.09290807903420525 1.8720144878945247E-5 2.6208202830523344E-4 58-59 0.10157550611315691 1.8720144878945247E-5 2.808021731841787E-4 60-61 0.11112278000141898 1.8720144878945247E-5 2.808021731841787E-4 62-63 0.12146566004703624 1.8720144878945247E-5 2.808021731841787E-4 64-65 0.13243566494609815 1.8720144878945247E-5 2.808021731841787E-4 66-67 0.14505304259450724 1.8720144878945247E-5 2.9952231806312394E-4 68-69 0.1592616325576267 1.8720144878945247E-5 2.9952231806312394E-4 70-71 0.17493039382130388 1.8720144878945247E-5 2.9952231806312394E-4 72-73 0.19285493254289393 1.8720144878945247E-5 3.088823905025966E-4 74-75 0.21339093147509686 1.8720144878945247E-5 3.182424629420692E-4 76-77 0.2359861463439838 1.8720144878945247E-5 3.276025353815418E-4 78-79 0.2624377110579334 1.8720144878945247E-5 3.556827526999597E-4 80-81 0.29269882525474844 1.8720144878945247E-5 3.556827526999597E-4 82-83 0.32573988096608675 1.8720144878945247E-5 3.556827526999597E-4 84-85 0.36295552898542993 1.8720144878945247E-5 3.556827526999597E-4 86-87 0.4043738495300963 1.8720144878945247E-5 3.8376297001837753E-4 88-89 0.45067812788816736 1.8720144878945247E-5 3.9312304245785017E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 5110 0.0 88.792885 9 CGGGAGA 7220 0.0 85.092735 4 AGAGCGG 5530 0.0 84.36637 8 GTTCGGA 1335 0.0 83.88893 2 CGGAAAG 2990 0.0 82.98169 5 TCGGGAG 7785 0.0 82.82112 3 AAGCGGC 1540 0.0 81.89324 9 GAGAGCG 7075 0.0 80.35567 7 TCGGAGA 1870 0.0 79.40771 4 ATCCGGA 1420 0.0 78.19904 2 CGGAGAG 3010 0.0 77.229164 5 ATCGGGA 9825 0.0 74.42931 2 ATTCGGA 1230 0.0 73.68892 2 TTCGGAG 1490 0.0 72.5987 3 CCGGAGA 1415 0.0 72.0845 4 GAGCGTC 11495 0.0 70.86003 9 GATCGGG 10465 0.0 70.838394 1 AGAGCGT 11640 0.0 70.83469 8 GGATCGG 3305 0.0 70.79496 1 GAACCGG 845 0.0 70.76862 1 >>END_MODULE