FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n02_52370180-2.342000000358cd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n02_52370180-2.342000000358cd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5341839
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT97490.18250269242483722Illumina Single End PCR Primer 1 (97% over 34bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC94320.17656840649821157No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC82920.155227441336214No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG81940.15339286713807737No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG70600.13216422284535342No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA68000.1272969851768277No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA55310.10354112132544616No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC51100.088.7928859
CGGGAGA72200.085.0927354
AGAGCGG55300.084.366378
GTTCGGA13350.083.888932
CGGAAAG29900.082.981695
TCGGGAG77850.082.821123
AAGCGGC15400.081.893249
GAGAGCG70750.080.355677
TCGGAGA18700.079.407714
ATCCGGA14200.078.199042
CGGAGAG30100.077.2291645
ATCGGGA98250.074.429312
ATTCGGA12300.073.688922
TTCGGAG14900.072.59873
CCGGAGA14150.072.08454
GAGCGTC114950.070.860039
GATCGGG104650.070.8383941
AGAGCGT116400.070.834698
GGATCGG33050.070.794961
GAACCGG8450.070.768621
Produced by FastQC (version 0.11.2)