##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n02_52260024-1.3420000003582d.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6655324 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.638310771947392 33.0 33.0 33.0 27.0 33.0 2 31.485960413046758 33.0 33.0 33.0 27.0 33.0 3 31.407802835744736 33.0 33.0 33.0 27.0 33.0 4 35.04993295593122 37.0 37.0 37.0 33.0 37.0 5 35.07632896610293 37.0 37.0 37.0 33.0 37.0 6 35.213377290121414 37.0 37.0 37.0 33.0 37.0 7 34.993647191331334 37.0 37.0 37.0 33.0 37.0 8 35.05102441293617 37.0 37.0 37.0 33.0 37.0 9 34.85000324552193 37.0 37.0 37.0 33.0 37.0 10-11 34.80358739859997 37.0 37.0 37.0 33.0 37.0 12-13 34.79777768896 37.0 37.0 37.0 33.0 37.0 14-15 36.43729681680411 40.0 37.0 40.0 33.0 40.0 16-17 36.37327776979753 40.0 37.0 40.0 33.0 40.0 18-19 36.35180901786299 40.0 37.0 40.0 33.0 40.0 20-21 36.27996773710792 40.0 37.0 40.0 33.0 40.0 22-23 36.53242419151945 40.0 37.0 40.0 33.0 40.0 24-25 36.21201146630878 40.0 37.0 40.0 27.0 40.0 26-27 36.127803920590495 40.0 37.0 40.0 27.0 40.0 28-29 36.059077514483135 40.0 37.0 40.0 27.0 40.0 30-31 36.028756526353945 40.0 37.0 40.0 27.0 40.0 32-33 35.90116266315509 40.0 37.0 40.0 27.0 40.0 34-35 35.890711331259006 40.0 37.0 40.0 27.0 40.0 36-37 35.78226642309225 37.0 37.0 40.0 27.0 40.0 38-39 35.79295591018559 37.0 37.0 40.0 27.0 40.0 40-41 35.595476568834215 37.0 37.0 40.0 27.0 40.0 42-43 35.492729354724126 37.0 37.0 40.0 27.0 40.0 44-45 35.340689574241615 37.0 37.0 40.0 27.0 40.0 46-47 35.25633192313402 37.0 37.0 40.0 27.0 40.0 48-49 35.133906403354665 37.0 37.0 40.0 27.0 40.0 50-51 33.99483090530228 37.0 35.0 38.5 24.5 38.5 52-53 34.437231079959446 37.0 35.0 38.5 27.0 40.0 54-55 35.19421586387079 37.0 37.0 40.0 27.0 40.0 56-57 35.276451755015984 37.0 37.0 40.0 27.0 40.0 58-59 34.995253499303715 37.0 37.0 40.0 27.0 40.0 60-61 34.74168124647275 37.0 33.0 40.0 27.0 40.0 62-63 34.46706847029536 37.0 33.0 40.0 27.0 40.0 64-65 34.232484624339854 37.0 33.0 38.5 27.0 40.0 66-67 34.01789386962979 37.0 33.0 37.0 27.0 40.0 68-69 33.8767898452427 37.0 33.0 37.0 27.0 40.0 70-71 33.49371615867236 37.0 33.0 37.0 24.5 38.5 72-73 33.3782331258403 37.0 33.0 37.0 27.0 37.0 74-75 33.21235788069822 37.0 33.0 37.0 27.0 37.0 76-77 32.9539503260848 37.0 33.0 37.0 22.0 37.0 78-79 32.68053824276624 37.0 33.0 37.0 22.0 37.0 80-81 32.40936053601598 37.0 33.0 37.0 22.0 37.0 82-83 32.17834819161321 37.0 33.0 37.0 22.0 37.0 84-85 32.012032021281 33.0 33.0 37.0 22.0 37.0 86-87 31.872007358319443 33.0 33.0 37.0 22.0 37.0 88-89 31.741953209190115 33.0 33.0 37.0 22.0 37.0 90-91 31.591640091451595 33.0 33.0 37.0 15.0 37.0 92-93 31.446150255043932 33.0 33.0 37.0 15.0 37.0 94-95 31.323043476170355 33.0 33.0 37.0 15.0 37.0 96-97 31.180643121206423 33.0 33.0 37.0 15.0 37.0 98-99 31.030387175740806 33.0 33.0 37.0 15.0 37.0 100-101 29.745736345818777 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.22971578635370093 2306 2 0.1758190133151345 2306 3 0.21928908010764658 2306 4 0.32290309786468896 2306 5 0.2257629791061433 2306 6 0.14934754361195957 2306 7 0.35854230748573457 2306 8 0.17105032683677024 2306 9 0.28994353445367693 2306 10-11 0.28538965766396274 2306 12-13 0.2656712829073413 2306 14-15 0.5000582709636703 2306 16-17 0.5019676586542445 2306 18-19 0.4789601931460581 2306 20-21 0.6088161254763094 2306 22-23 0.42255400688838307 2306 24-25 0.5371773402704108 2306 26-27 0.43858391121718654 2306 28-29 0.4733402902083057 2306 30-31 0.4528654234334084 2306 32-33 0.3388002950910547 2306 34-35 0.3253975954829116 2306 36-37 0.40322693437703094 2306 38-39 0.13205043238854586 2306 40-41 0.29080103783549305 2306 42-43 0.26211608825260413 2306 44-45 0.2547367173333299 2306 46-47 0.2730244301065241 2306 48-49 0.14384235554263824 2306 50-51 0.15074453922016318 2306 52-53 0.23646810361184833 2306 54-55 0.409003079914946 2306 56-57 0.33896614677800585 2306 58-59 0.42786953124549854 2306 60-61 0.4617308168007739 2306 62-63 0.35509095583581285 2306 64-65 0.3533101776018128 2306 66-67 0.2825028184531746 2306 68-69 0.1943785825307316 2306 70-71 0.25486321739757756 2306 72-73 0.20387008226058612 2306 74-75 0.16418756977822824 2306 76-77 0.3432457499847601 2306 78-79 0.5143916332769081 2306 80-81 0.44391128544304337 2306 82-83 0.3318828018178053 2306 84-85 0.2859351764979223 2306 86-87 0.32212535764169203 2306 88-89 0.42022821875244887 2306 90-91 0.3886963595656425 2306 92-93 0.39059414660675884 2306 94-95 0.33456167621915966 2306 96-97 0.3791243483098512 2306 98-99 0.2122996325110762 2306 100-101 0.07165111855413997 2307 1 0.20089599873051966 2307 2 0.18125013813234503 2307 3 0.11270157137699144 2307 4 0.20439576693009798 2307 5 0.20717057581943976 2307 6 0.17081861961553102 2307 7 0.34248176389946394 2307 8 0.23423802551622686 2307 9 0.24807765709798701 2307 10-11 0.2566324315213606 2307 12-13 0.267769958148385 2307 14-15 0.4478912466515297 2307 16-17 0.4198873581081699 2307 18-19 0.5331254444203921 2307 20-21 0.6119036582180257 2307 22-23 0.4722744570157289 2307 24-25 0.5129024199875403 2307 26-27 0.36709693241102315 2307 28-29 0.38950953512119924 2307 30-31 0.3516605347654931 2307 32-33 0.35944178293699736 2307 34-35 0.3147745515095295 2307 36-37 0.30355640070970225 2307 38-39 0.17940128851355297 2307 40-41 0.14811851094532358 2307 42-43 0.1632961172283558 2307 44-45 0.17900112825274306 2307 46-47 0.20724216947769492 2307 48-49 0.12795204112162395 2307 50-51 0.07173562327408689 2307 52-53 0.10504244028520304 2307 54-55 0.12154518272556203 2307 56-57 0.1458188268989673 2307 58-59 0.23465058038161857 2307 60-61 0.27733936927491953 2307 62-63 0.13841496896313998 2307 64-65 0.14560799261143842 2307 66-67 0.10543546819391025 2307 68-69 0.18301423227042335 2307 70-71 0.04931446653979066 2307 72-73 0.06811540981689035 2307 74-75 0.11359425527782463 2307 76-77 0.23921196026968516 2307 78-79 0.2096889686284058 2307 80-81 0.15974294369993913 2307 82-83 0.12949848344514692 2307 84-85 0.061680261409797765 2307 86-87 0.20328474756288628 2307 88-89 0.3811807853658422 2307 90-91 0.38249808741278457 2307 92-93 0.2582485419459246 2307 94-95 0.23096282051307782 2307 96-97 0.2740449714455231 2307 98-99 0.2788522580380395 2307 100-101 0.14723785515023735 2201 1 0.28473772151260235 2201 2 0.2777477743012895 2201 3 0.3181174321120004 2201 4 0.3826138150024221 2201 5 0.29095643007877925 2201 6 0.2970383098838312 2201 7 0.4358545720122535 2201 8 0.30740293500498694 2201 9 0.36542652639526807 2201 10-11 0.3681570073226723 2201 12-13 0.42771180409516774 2201 14-15 0.5762275920882942 2201 16-17 0.6027357216260256 2201 18-19 0.5821306037938214 2201 20-21 0.6646647060067679 2201 22-23 0.5676828686143764 2201 24-25 0.5846145362617818 2201 26-27 0.48010964249994714 2201 28-29 0.5150888536098748 2201 30-31 0.5527613986113593 2201 32-33 0.4540934300002988 2201 34-35 0.3839857048982509 2201 36-37 0.4324188414023027 2201 38-39 0.39357665642047124 2201 40-41 0.37920786248953675 2201 42-43 0.42013831827885895 2201 44-45 0.43738373451932233 2201 46-47 0.43812942087334505 2201 48-49 0.3970594991461667 2201 50-51 0.4409471999091892 2201 52-53 0.5108559409983755 2201 54-55 0.5374867577946603 2201 56-57 0.5417262459848402 2201 58-59 0.5565002517308031 2201 60-61 0.5094237730689031 2201 62-63 0.5022384075014159 2201 64-65 0.5099805933334878 2201 66-67 0.5232367878761366 2201 68-69 0.441691418020568 2201 70-71 0.5389385814257892 2201 72-73 0.5028999139202668 2201 74-75 0.4088505098012689 2201 76-77 0.4127038275904056 2201 78-79 0.5032463121647979 2201 80-81 0.40194864990768764 2201 82-83 0.36593978552032524 2201 84-85 0.33346771554047194 2201 86-87 0.4439864477436082 2201 88-89 0.4500673087777187 2201 90-91 0.4224940242474915 2201 92-93 0.35532179319767465 2201 94-95 0.3608224296873672 2201 96-97 0.3442211265907211 2201 98-99 0.3714871360795975 2201 100-101 0.15623882438445946 1101 1 0.2010890621364183 1101 2 0.163839005473438 1101 3 0.2786916109884352 1101 4 0.3356220572746622 1101 5 0.292595162638392 1101 6 0.23761330707742445 1101 7 0.43146737790637246 1101 8 0.28147633945264516 1101 9 0.290186915088924 1101 10-11 0.3394673324218971 1101 12-13 0.3452882424361903 1101 14-15 0.48781744593475906 1101 16-17 0.5048273262865166 1101 18-19 0.5589490776183368 1101 20-21 0.5722819797726757 1101 22-23 0.42773502059615254 1101 24-25 0.34720864710598676 1101 26-27 0.4249892676967093 1101 28-29 0.357646556334835 1101 30-31 0.3361655420238989 1101 32-33 0.21963778401409684 1101 34-35 0.23895285505980723 1101 36-37 0.25079628634337325 1101 38-39 0.24834704327115986 1101 40-41 0.16356406895305042 1101 42-43 0.2539214754233825 1101 44-45 0.2310825175698099 1101 46-47 0.1928664805339153 1101 48-49 0.2127119654456635 1101 50-51 0.2851876109981575 1101 52-53 0.32531093652735166 1101 54-55 0.4571698732189944 1101 56-57 0.4166926477708657 1101 58-59 0.4928415417413561 1101 60-61 0.49587518618722726 1101 62-63 0.41951647481814547 1101 64-65 0.40609895486671377 1101 66-67 0.359992482087101 1101 68-69 0.4097533737216281 1101 70-71 0.46336314534843126 1101 72-73 0.42341008920449497 1101 74-75 0.37856273843208754 1101 76-77 0.40697621949298934 1101 78-79 0.5110444550045088 1101 80-81 0.4474785225054987 1101 82-83 0.344024710055038 1101 84-85 0.4572139330093066 1101 86-87 0.4695918887833379 1101 88-89 0.39772989423328653 1101 90-91 0.4970389052715234 1101 92-93 0.45536868906541983 1101 94-95 0.48969849905128626 1101 96-97 0.42201971006274164 1101 98-99 0.48891031480810554 1101 100-101 0.32600223280829965 2304 1 0.19032446462054153 2304 2 0.07859539064964238 2304 3 0.17410221015552096 2304 4 0.22671340080741942 2304 5 0.23237377588271357 2304 6 0.20300692785813368 2304 7 0.1970569806084157 2304 8 0.17286994443086456 2304 9 0.16180716812404938 2304 10-11 0.28086263384441423 2304 12-13 0.2320417081789401 2304 14-15 0.3437387259616216 2304 16-17 0.310216922078844 2304 18-19 0.4144393058667859 2304 20-21 0.4402033335445239 2304 22-23 0.352192820730842 2304 24-25 0.24940307139708295 2304 26-27 0.28893138284824715 2304 28-29 0.17370548538736585 2304 30-31 0.0799096519150666 2304 32-33 0.0972531627541855 2304 34-35 0.04941948259205731 2304 36-37 0.1213161280023769 2304 38-39 0.06887296579214564 2304 40-41 0.08197786409725438 2304 42-43 0.11005319200656061 2304 44-45 0.1892547390458006 2304 46-47 0.10964228511716811 2304 48-49 0.1070859519363907 2304 50-51 0.15460146392991447 2304 52-53 0.19260194117405405 2304 54-55 0.2698085536672963 2304 56-57 0.3269548653845433 2304 58-59 0.3092006407326764 2304 60-61 0.2688897615098895 2304 62-63 0.2885530664245124 2304 64-65 0.22014676856831272 2304 66-67 0.2460740319462218 2304 68-69 0.24610916658314608 2304 70-71 0.2596460112455148 2304 72-73 0.29426745356215633 2304 74-75 0.35659995082861684 2304 76-77 0.275976465361218 2304 78-79 0.32979755450119796 2304 80-81 0.34831495475454943 2304 82-83 0.21319157928800792 2304 84-85 0.2161214972452825 2304 86-87 0.3247001924211439 2304 88-89 0.3144497703789 2304 90-91 0.1726520614371978 2304 92-93 0.29556964722335977 2304 94-95 0.2227849416416774 2304 96-97 0.28224610094178715 2304 98-99 0.2939321462645843 2304 100-101 0.14405869016472295 2202 1 0.19347782111060852 2202 2 0.15711268155204294 2202 3 0.12174001850282323 2202 4 0.1914832357362215 2202 5 0.18562938792901917 2202 6 0.1734554606410228 2202 7 0.32934833158974897 2202 8 0.1908324486076296 2202 9 0.17179451598579476 2202 10-11 0.28591275142947126 2202 12-13 0.3026635279251835 2202 14-15 0.2958545859220223 2202 16-17 0.32188832535002376 2202 18-19 0.36344122805930823 2202 20-21 0.43019161389217686 2202 22-23 0.35109587822496025 2202 24-25 0.23109081436440704 2202 26-27 0.29168970440188247 2202 28-29 0.29235273091584446 2202 30-31 0.28046243238315327 2202 32-33 0.27155505223906573 2202 34-35 0.08876078020270484 2202 36-37 0.1747647363731275 2202 38-39 0.1515809331071054 2202 40-41 0.09076520150385647 2202 42-43 0.08020431830118468 2202 44-45 0.1188928850660318 2202 46-47 0.24286407335667803 2202 48-49 0.15753068413279436 2202 50-51 0.04933800487346218 2202 52-53 0.18439366338368046 2202 54-55 0.3212838240705409 2202 56-57 0.2496074204312393 2202 58-59 0.23192588729396135 2202 60-61 0.35219183537266474 2202 62-63 0.32148062710496816 2202 64-65 0.27607684907999186 2202 66-67 0.30358093198867664 2202 68-69 0.2521968866621762 2202 70-71 0.28539357177056246 2202 72-73 0.2948129578145071 2202 74-75 0.21991376553660302 2202 76-77 0.2137092954347395 2202 78-79 0.32447602700451483 2202 80-81 0.3427887748596561 2202 82-83 0.32256115524003803 2202 84-85 0.37754222428758766 2202 86-87 0.3787429779825935 2202 88-89 0.411091337633394 2202 90-91 0.2799961649826912 2202 92-93 0.3071865848407569 2202 94-95 0.34521045049994115 2202 96-97 0.3072194155971495 2202 98-99 0.3246375647847408 2202 100-101 0.1530989996935297 1102 1 -0.05349172266826585 1102 2 -0.07061238287058913 1102 3 -0.03180957785113847 1102 4 -0.015135962986214224 1102 5 -0.05433662491921609 1102 6 -0.06782337399631899 1102 7 0.016257963615963433 1102 8 -0.024829769397527457 1102 9 -0.008622170124318984 1102 10-11 0.04700647145414649 1102 12-13 0.0031339298083423728 1102 14-15 0.04287798805924581 1102 16-17 2.0920191263229526E-4 1102 18-19 0.04063956940334634 1102 20-21 0.09874785990626833 1102 22-23 0.022022734556003343 1102 24-25 -0.10438829818465223 1102 26-27 -0.10184463532446131 1102 28-29 -0.17314804163725483 1102 30-31 -0.10480280220856031 1102 32-33 -0.22286817724031494 1102 34-35 -0.1586830541494635 1102 36-37 -0.16714808437899364 1102 38-39 -0.18735652120078328 1102 40-41 -0.13298147366607793 1102 42-43 -0.16112027325672784 1102 44-45 -0.1690860719354319 1102 46-47 -0.1677590503503339 1102 48-49 -0.19297568813410493 1102 50-51 -0.1737137613695907 1102 52-53 -0.07815358420143781 1102 54-55 0.009473029585230108 1102 56-57 0.10449524468317861 1102 58-59 0.01966005125150616 1102 60-61 0.1526293130126959 1102 62-63 0.059586679462292125 1102 64-65 0.04929181068565924 1102 66-67 0.05820601041059348 1102 68-69 -0.03377252490122373 1102 70-71 -0.06365454169623064 1102 72-73 0.06410403664084896 1102 74-75 0.1310080040533066 1102 76-77 0.0410732301669583 1102 78-79 0.0036187134153635725 1102 80-81 -0.015759797472576054 1102 82-83 0.00799360350750078 1102 84-85 0.07089061981403688 1102 86-87 -0.008257843827255584 1102 88-89 0.11779187479112352 1102 90-91 0.18750853101731835 1102 92-93 0.09801685907118696 1102 94-95 0.1382574077295331 1102 96-97 0.08009314464358752 1102 98-99 -0.0026036817737669082 1102 100-101 -0.006152907060865687 2305 1 0.07878892201435761 2305 2 0.0651738947949454 2305 3 0.040779996279372455 2305 4 0.11496511110434682 2305 5 0.07454140131096665 2305 6 0.05375232028166721 2305 7 0.09328429652696713 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-1.1614395275179419 1213 58-59 -1.1625582251239095 1213 60-61 -1.0415524211049956 1213 62-63 -1.1341371287498774 1213 64-65 -1.1191419613908593 1213 66-67 -0.976211884277923 1213 68-69 -0.9518542039300968 1213 70-71 -1.8056510035504942 1213 72-73 -1.3023851488970166 1213 74-75 -1.2291807243784376 1213 76-77 -1.1599587016802886 1213 78-79 -1.1128183344791296 1213 80-81 -1.0808350363547952 1213 82-83 -1.0667055393365246 1213 84-85 -1.1066149382554933 1213 86-87 -1.048932009788917 1213 88-89 -1.2190011518810522 1213 90-91 -1.141154138430025 1213 92-93 -1.1097302536838498 1213 94-95 -1.1952829186062104 1213 96-97 -1.1381072376991277 1213 98-99 -1.1452662921973626 1213 100-101 -1.0083239152520491 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 701.0 3 334.0 4 404.0 5 411.0 6 1441.0 7 5834.0 8 22364.0 9 58989.0 10 70822.0 11 53983.0 12 34458.0 13 24688.0 14 19282.0 15 17114.0 16 16912.0 17 17689.0 18 19217.0 19 21010.0 20 22993.0 21 25747.0 22 28492.0 23 31338.0 24 36160.0 25 41769.0 26 48898.0 27 59220.0 28 71871.0 29 88518.0 30 109431.0 31 142715.0 32 195490.0 33 279071.0 34 420284.0 35 713341.0 36 1386046.0 37 2106989.0 38 460748.0 39 550.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.38348125670081 25.299682937484402 23.537036942254037 15.779798863560753 2 30.7187867043358 24.17704391642968 31.613320711394472 13.490848667840053 3 36.68252061173374 20.522617378684664 25.86561155617461 16.929250453406976 4 28.992535713237764 19.07303203827469 33.48424419300159 18.45018805548596 5 32.42183826181615 17.764041665393858 32.13066023376404 17.683459839025954 6 27.21910325782372 18.510745695365234 37.219772771553636 17.050378275257405 7 33.194416973195665 19.099613213173463 29.246153001527443 18.459816812103426 8 28.474784220641396 27.380528596451732 25.965154966658744 18.17953221624813 9 30.11660612324269 25.53467157511059 25.25251826454283 19.096204037103888 10-11 25.167299154743723 28.456851136362182 28.70952809951423 17.666321609379864 12-13 26.267159786384305 25.33869144707797 28.24632113445308 20.147827632084645 14-15 24.74264783846744 25.827268101807753 28.949559502737493 20.48052455698731 16-17 24.684346932973263 26.608512295455167 29.801681452774474 18.905459318797096 18-19 24.104820258759535 26.75530540656863 29.880374018418205 19.259500316253632 20-21 23.60618796145325 27.802111251423433 29.185579067790652 19.40612171933266 22-23 24.74858872694721 27.08647798753963 28.5782388963797 19.58669438913346 24-25 23.229151157265164 27.934437878163703 29.026702218208218 19.809708746362922 26-27 22.44857152823489 28.685480810602325 29.020674001172736 19.845273659990053 28-29 22.591884202439623 28.522584121358523 28.546286370559805 20.339245305642045 30-31 23.288418074118386 27.925015327936514 28.648150549478853 20.13841604846625 32-33 22.118498049880102 27.643983387861965 29.47922330255053 20.7582952597074 34-35 21.16339438366474 27.868650469172714 31.09165548064568 19.876299666516868 36-37 21.424160079051383 28.260560770750985 29.97730737250554 20.337971777692086 38-39 21.20087004830463 27.9035865952298 28.8527302002806 22.04281315618497 40-41 21.85033076863973 27.599882185799924 29.079497466693383 21.470289578866968 42-43 22.82252690468146 27.762154689222186 29.59912047227221 19.816197933824142 44-45 21.763650983317362 28.58638929613632 29.878111525648904 19.771848194897412 46-47 22.317542111114694 28.784281631443847 29.142133055179414 19.75604320226204 48-49 21.306221383919567 28.752794279413024 29.506257212167565 20.434727124499847 50-51 20.806871767067793 28.34267126186881 29.438374116982963 21.412082854080435 52-53 22.606027358783198 27.87495779791021 28.57654658556934 20.94246825773725 54-55 23.29438213791497 27.8891473147915 28.478315185979646 20.338155361313888 56-57 23.617229481394993 27.533385753367728 28.026599664249602 20.82278510098767 58-59 22.91359885064433 27.54574115572558 28.251011155174165 21.28964883845593 60-61 21.37266040739373 27.876905400639384 28.00753390184283 22.74290029012406 62-63 22.29403127921191 27.93272075404831 28.26663788949249 21.506610077247284 64-65 21.663002572873093 28.43228093490946 29.060521029769752 20.844195462447697 66-67 21.66168265249016 28.326542336305316 28.373454101443134 21.63832090976139 68-69 21.588388254977094 27.703580532572268 30.088558693406796 20.619472519043843 70-71 21.325796877148555 29.547541529755318 29.01717367982617 20.10948791326996 72-73 21.273382739721015 31.181993647965296 27.519833825145785 20.024789787167908 74-75 21.861919912645668 30.739936366888188 27.14980138185515 20.248342338610993 76-77 21.92584648072121 29.959356514927528 27.64775877209398 20.467038232257288 78-79 21.72258492266011 29.755629805973253 28.310091252304048 20.211694019062584 80-81 21.19941122767495 30.157244446707203 28.060036762119744 20.583307563498103 82-83 20.387588945478914 30.099008439085566 28.405902960459905 21.107499654975616 84-85 20.529702899070926 29.862142740330448 28.44436938414658 21.16378497645205 86-87 20.35397250161484 29.917768491543523 29.06459163899942 20.663667367842216 88-89 20.46849419617441 30.225872306345934 28.86597425614027 20.439659241339385 90-91 20.90563988463992 29.585363520576287 28.39554135857657 21.11345523620722 92-93 20.59208502067278 30.178394494728906 27.97761288551273 21.251907599085587 94-95 20.30316883519013 30.418947333068925 27.987367701644743 21.290516130096197 96-97 20.671936812464864 30.767712648997474 27.715560011848865 20.84479052668879 98-99 20.95503194315486 30.185919826085595 27.603700019341233 21.255348211418315 100-101 21.0278775524131 30.03434915532623 27.169177818925426 21.768595473335246 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 138.0 1 198.0 2 298.0 3 305.5 4 276.0 5 324.0 6 459.0 7 685.5 8 1002.0 9 1391.5 10 1797.5 11 2106.5 12 2230.5 13 2172.5 14 1971.5 15 1735.0 16 1503.5 17 1308.0 18 1273.5 19 1351.5 20 1502.5 21 1820.5 22 2232.5 23 2740.5 24 3436.0 25 4419.5 26 6043.0 27 8362.0 28 10973.5 29 13722.0 30 17022.5 31 21595.0 32 33143.5 33 55901.5 34 92235.5 35 144845.5 36 216817.5 37 305534.5 38 391036.0 39 427330.0 40 430310.0 41 412675.5 42 390102.0 43 403756.0 44 408143.0 45 399713.0 46 404998.5 47 370713.5 48 299281.0 49 249564.5 50 210522.5 51 181234.5 52 160156.5 53 132391.5 54 100045.0 55 71405.5 56 53622.5 57 41584.5 58 33513.0 59 26879.5 60 21044.0 61 16339.5 62 12387.5 63 9664.0 64 7775.0 65 6025.0 66 4412.0 67 3252.5 68 2504.5 69 1893.5 70 1396.5 71 1055.0 72 803.5 73 594.0 74 444.5 75 349.5 76 293.0 77 250.5 78 206.5 79 155.0 80 116.0 81 93.5 82 75.0 83 67.0 84 66.0 85 57.5 86 38.0 87 27.5 88 25.5 89 26.0 90 20.0 91 13.0 92 14.5 93 15.5 94 11.0 95 8.0 96 7.5 97 6.5 98 2.5 99 0.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18736878925804365 2 0.17827832273830696 3 0.15145769011395988 4 0.14543243875129144 5 0.14882821632725918 6 0.1308576411907219 7 0.10456290332371497 8 0.09097979301984396 9 0.09168599455112929 10-11 0.0711836117971116 12-13 0.09155827725291811 14-15 0.09557761575544632 16-17 0.08858321548282247 18-19 0.0841131100454313 20-21 0.08228750395923624 22-23 0.10225647917366606 24-25 0.445335193297877 26-27 0.2810306455403223 28-29 0.12434405898195189 30-31 0.23270091734076356 32-33 0.26335306891144594 34-35 0.24728322768358085 36-37 0.24948447288216172 38-39 0.26687656378562485 40-41 0.26676387205190916 42-43 0.29284825201597997 44-45 0.2696337548705367 46-47 0.2883405826673502 48-49 0.2720077940608151 50-51 0.287393972104138 52-53 0.286995794645009 54-55 0.26275955911387633 56-57 0.27870168304353027 58-59 0.2684467352753976 60-61 0.24442837042944868 62-63 0.23744148293907255 64-65 0.23537546782095053 66-67 0.24438329373596235 68-69 0.24886842473784898 70-71 0.2357060302398501 72-73 0.22672825545382913 74-75 0.23588633701379524 76-77 0.23681040923026436 78-79 0.23935724241224018 80-81 0.2467948968374793 82-83 0.27214302414127395 84-85 0.2659149276579172 86-87 0.26537400733608163 88-89 0.2636535802013546 90-91 0.25511905956794895 92-93 0.23188953685801023 94-95 0.23080018343209135 96-97 0.22578915767286464 98-99 0.2502658022359242 100-101 0.2579814296043288 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6655324.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.26147204019225 #Duplication Level Percentage of deduplicated Percentage of total 1 87.22424325108915 31.62879457876297 2 5.3549658739614685 3.8835789062967496 3 2.1038067194423733 2.288613856050846 4 1.1693320399931695 1.6960680429565316 5 0.7751369164543341 1.405380281166484 6 0.42264872227011563 0.9195518895252467 7 0.3070810953861068 0.7794648788090445 8 0.209133368146794 0.6066787025380963 9 0.22589066816061498 0.7372015332881837 >10 1.7036484543974328 12.400655267413343 >50 0.2285545834315064 5.7329148042219344 >100 0.21787894612727254 17.28588662343856 >500 0.04061406131044544 10.196070737483092 >1k 0.016813031953552583 9.854615111626028 >5k 2.522678756919532E-4 0.5845247864226024 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 7802 0.11722945419336459 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 7064 0.10614058759573539 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 7062 0.10611053646674452 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0014274286270660901 0.0 0.0 2 0.001562658707524983 0.0 0.0 3 0.0017429654814701734 0.0 0.0 4 0.0019382978199107962 0.0 0.0 5 0.002359013625782907 0.0 0.0 6 0.002779729431655018 0.0 0.0 7 0.0032605474955088587 0.0 0.0 8 0.003906646768812458 0.0 0.0 9 0.004552746042116056 0.0 0.0 10-11 0.006889221321155815 0.0 0.0 12-13 0.009293311640425019 1.5025564495432529E-5 0.0 14-15 0.011667350830703358 1.5025564495432529E-5 0.0 16-17 0.013928698287265954 3.0051128990865058E-5 0.0 18-19 0.016693402154425538 3.0051128990865058E-5 0.0 20-21 0.01966846392452118 3.0051128990865058E-5 0.0 22-23 0.02282383246856201 3.0051128990865058E-5 0.0 24-25 0.02626468673801606 3.0051128990865058E-5 0.0 26-27 0.02980320717669042 3.0051128990865058E-5 0.0 28-29 0.03425828704958617 3.0051128990865058E-5 0.0 30-31 0.06158227608453022 3.0051128990865058E-5 0.0 32-33 0.06837383123646573 3.0051128990865058E-5 0.0 34-35 0.0735351126406468 3.0051128990865058E-5 0.0 36-37 0.07935000610037918 3.0051128990865058E-5 0.0 38-39 0.08558561536598369 3.0051128990865058E-5 0.0 40-41 0.09354165176631521 3.0051128990865058E-5 0.0 42-43 0.10195596788375742 3.0051128990865058E-5 0.0 44-45 0.11160238028982511 3.0051128990865058E-5 0.0 46-47 0.12238322281529795 3.0051128990865058E-5 0.0 48-49 0.13482439021751608 3.0051128990865058E-5 0.0 50-51 0.14802434862675357 3.0051128990865058E-5 0.0 52-53 0.16246391610686423 3.0051128990865058E-5 0.0 54-55 0.1779252219726643 3.0051128990865058E-5 0.0 56-57 0.1961286933588808 3.0051128990865058E-5 0.0 58-59 0.21546659486450248 3.0051128990865058E-5 0.0 60-61 0.23791478822067866 3.0051128990865058E-5 0.0 62-63 0.26311265987951904 3.0051128990865058E-5 0.0 64-65 0.29115787601024384 3.0051128990865058E-5 0.0 66-67 0.3208183403242276 3.0051128990865058E-5 0.0 68-69 0.35419011906858333 4.507669348629759E-5 0.0 70-71 0.3913182889367971 4.507669348629759E-5 0.0 72-73 0.43230802888033704 4.507669348629759E-5 0.0 74-75 0.47759508026957065 4.507669348629759E-5 0.0 76-77 0.5295234311657855 4.507669348629759E-5 0.0 78-79 0.5852231987503538 4.507669348629759E-5 0.0 80-81 0.6476469064466283 4.507669348629759E-5 0.0 82-83 0.7162836850617641 4.507669348629759E-5 0.0 84-85 0.7926886805210385 4.507669348629759E-5 0.0 86-87 0.8772901214125713 4.507669348629759E-5 0.0 88-89 0.9733861191431101 4.507669348629759E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 2060 0.0 77.11418 9 CGGGAGA 3485 0.0 71.89384 4 AGAGCGG 2325 0.0 71.385216 8 AAGCGGC 945 0.0 64.73133 9 CGGAAAG 1505 0.0 64.32024 5 TCGGGAA 1385 0.0 61.675037 3 ACCGGAA 585 0.0 60.8404 3 TCGGGAG 3975 0.0 59.095543 3 GAGAGCG 3600 0.0 57.433697 7 ATTCGGA 490 0.0 57.14934 2 GATCCGG 700 0.0 55.606224 1 GAACCGG 530 0.0 54.633823 1 AAGCGTC 525 0.0 52.38722 9 TCGGAAA 1240 0.0 50.514114 4 AACCGGA 530 0.0 49.254074 2 TTGTGTT 69500 0.0 48.68448 1 TCGGAGA 995 0.0 47.691105 4 GGATCGG 1720 0.0 47.46873 1 CGGAGAG 1730 0.0 46.629074 5 GGTCCGG 445 0.0 45.86866 1 >>END_MODULE