FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n02_52260024-1.3420000003582d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n02_52260024-1.3420000003582d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6655324
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA78020.11722945419336459No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG70640.10614058759573539No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC70620.10611053646674452No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC20600.077.114189
CGGGAGA34850.071.893844
AGAGCGG23250.071.3852168
AAGCGGC9450.064.731339
CGGAAAG15050.064.320245
TCGGGAA13850.061.6750373
ACCGGAA5850.060.84043
TCGGGAG39750.059.0955433
GAGAGCG36000.057.4336977
ATTCGGA4900.057.149342
GATCCGG7000.055.6062241
GAACCGG5300.054.6338231
AAGCGTC5250.052.387229
TCGGAAA12400.050.5141144
AACCGGA5300.049.2540742
TTGTGTT695000.048.684481
TCGGAGA9950.047.6911054
GGATCGG17200.047.468731
CGGAGAG17300.046.6290745
GGTCCGG4450.045.868661
Produced by FastQC (version 0.11.2)