##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n02_52150223-1.3420000003573b.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3219527 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.598445361694434 33.0 33.0 33.0 27.0 33.0 2 31.346262354687504 33.0 33.0 33.0 27.0 33.0 3 31.272007968872447 33.0 33.0 33.0 27.0 33.0 4 34.8449120010486 37.0 37.0 37.0 33.0 37.0 5 34.88421063094051 37.0 37.0 37.0 33.0 37.0 6 35.145333771078796 37.0 37.0 37.0 33.0 37.0 7 34.80759844536169 37.0 37.0 37.0 33.0 37.0 8 34.97223660494228 37.0 37.0 37.0 33.0 37.0 9 34.66921414232588 37.0 37.0 37.0 33.0 37.0 10-11 34.64162095860665 37.0 37.0 37.0 33.0 37.0 12-13 34.6168820761559 37.0 37.0 37.0 33.0 37.0 14-15 36.24611130765482 40.0 37.0 40.0 33.0 40.0 16-17 36.18732052876091 40.0 37.0 40.0 30.0 40.0 18-19 36.16168166938808 40.0 37.0 40.0 27.0 40.0 20-21 36.08221704616858 40.0 37.0 40.0 27.0 40.0 22-23 36.474932963755236 40.0 37.0 40.0 30.0 40.0 24-25 36.045647543878346 40.0 37.0 40.0 27.0 40.0 26-27 35.99318160711185 40.0 37.0 40.0 27.0 40.0 28-29 35.834046429801646 40.0 37.0 40.0 27.0 40.0 30-31 35.76572754320743 40.0 37.0 40.0 27.0 40.0 32-33 35.66160029097442 40.0 37.0 40.0 27.0 40.0 34-35 35.65813875764981 40.0 37.0 40.0 27.0 40.0 36-37 35.54933892463086 40.0 37.0 40.0 27.0 40.0 38-39 35.436474985300634 37.0 37.0 40.0 27.0 40.0 40-41 35.39311613165536 37.0 37.0 40.0 27.0 40.0 42-43 35.29832782890158 37.0 37.0 40.0 27.0 40.0 44-45 35.11927140228984 37.0 37.0 40.0 22.0 40.0 46-47 35.023778182323056 37.0 37.0 40.0 22.0 40.0 48-49 34.91217125993974 37.0 37.0 40.0 22.0 40.0 50-51 33.8886086372315 37.0 35.0 38.5 22.0 38.5 52-53 34.298760345852045 37.0 35.0 38.5 22.0 40.0 54-55 35.00397449687485 37.0 37.0 40.0 22.0 40.0 56-57 35.15742529880942 37.0 37.0 40.0 24.5 40.0 58-59 34.856780669955555 37.0 37.0 40.0 22.0 40.0 60-61 34.702906513907166 37.0 35.0 40.0 22.0 40.0 62-63 34.43973151956794 37.0 33.0 40.0 22.0 40.0 64-65 34.27129419942743 37.0 33.0 40.0 22.0 40.0 66-67 34.119749267516625 37.0 33.0 40.0 22.0 40.0 68-69 34.06166293992875 37.0 33.0 40.0 22.0 40.0 70-71 33.676467071094606 37.0 33.0 37.0 22.0 40.0 72-73 33.678855931321586 37.0 33.0 37.0 22.0 40.0 74-75 33.57225020942518 37.0 33.0 37.0 22.0 40.0 76-77 33.26584588357234 37.0 33.0 37.0 22.0 40.0 78-79 32.877943561274684 37.0 33.0 37.0 22.0 37.0 80-81 32.469161619082556 37.0 33.0 37.0 22.0 37.0 82-83 32.13689728335871 37.0 33.0 37.0 15.0 37.0 84-85 31.896395495363137 37.0 33.0 37.0 15.0 37.0 86-87 31.707687495709774 37.0 33.0 37.0 15.0 37.0 88-89 31.511783563237707 33.0 33.0 37.0 15.0 37.0 90-91 31.31979324913256 33.0 33.0 37.0 15.0 37.0 92-93 31.15653199988694 33.0 33.0 37.0 15.0 37.0 94-95 31.01296230781727 33.0 33.0 37.0 15.0 37.0 96-97 30.86399694737767 33.0 33.0 37.0 15.0 37.0 98-99 30.712749419402293 33.0 27.0 37.0 15.0 37.0 100-101 29.4590385482091 33.0 27.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -0.00295443000300466 2306 2 0.06637542051398881 2306 3 -0.07597890648014172 2306 4 0.03182483795103508 2306 5 0.0593045071988243 2306 6 -0.06391302673767996 2306 7 0.05260131521647082 2306 8 -0.20639090801824267 2306 9 0.07389654439409554 2306 10-11 0.08730141470461916 2306 12-13 0.013282593945525889 2306 14-15 0.3386398623975211 2306 16-17 0.24553913466014876 2306 18-19 0.21892886602227435 2306 20-21 0.24856205577123802 2306 22-23 0.12722351251029806 2306 24-25 4.829120785174723E-4 2306 26-27 0.05661880125175145 2306 28-29 0.07723908173878868 2306 30-31 0.16295238818901936 2306 32-33 -0.015536871297108235 2306 34-35 0.045519985784295613 2306 36-37 -0.10906766711091365 2306 38-39 -0.16833797963221997 2306 40-41 -0.17001065691402317 2306 42-43 -0.16403453703308202 2306 44-45 -0.10323029435069486 2306 46-47 -0.01762065081438635 2306 48-49 -0.17950749070616467 2306 50-51 -0.15022296563879678 2306 52-53 -0.19834946511591767 2306 54-55 -0.05557175866162112 2306 56-57 -0.07691058059079126 2306 58-59 -0.046169795119567425 2306 60-61 0.008635149166948963 2306 62-63 0.10418441859133765 2306 64-65 0.0016411336365322882 2306 66-67 -0.04244971547072396 2306 68-69 -0.08031798019748493 2306 70-71 0.01271555584691697 2306 72-73 -0.21613041467597327 2306 74-75 -0.1006522499537752 2306 76-77 0.16434853619018952 2306 78-79 0.17583717506820307 2306 80-81 0.14355577810720632 2306 82-83 0.15993224890074487 2306 84-85 0.16650318238731643 2306 86-87 0.05005445801913311 2306 88-89 0.0678455082940701 2306 90-91 -0.0026597510444581474 2306 92-93 0.1494808036825077 2306 94-95 -0.0510262346803394 2306 96-97 -0.08650389124227331 2306 98-99 -0.12726284236907404 2306 100-101 -0.3150557453744902 2307 1 0.20854487486075968 2307 2 0.19293217902621507 2307 3 0.1508143078225146 2307 4 0.3768267901709095 2307 5 0.28929242897866203 2307 6 0.19168546549175147 2307 7 0.5400551096385939 2307 8 0.17328301652754874 2307 9 0.2589514802262727 2307 10-11 0.2957629194830034 2307 12-13 0.30743469194331396 2307 14-15 0.5549252988372544 2307 16-17 0.36782564025955367 2307 18-19 0.5419149778935903 2307 20-21 0.5148831612750442 2307 22-23 0.3161979402544475 2307 24-25 0.3190482494111677 2307 26-27 0.3549741016199306 2307 28-29 0.3418620296272863 2307 30-31 0.29095871557081665 2307 32-33 0.32196043755867265 2307 34-35 0.25217431880016505 2307 36-37 0.15611312304552172 2307 38-39 0.06540203576659565 2307 40-41 -0.004328310455200324 2307 42-43 0.06063449784049624 2307 44-45 0.1323752769564237 2307 46-47 0.14443412446139092 2307 48-49 0.09762015528156809 2307 50-51 -0.060331906805821234 2307 52-53 -0.07578289806719596 2307 54-55 0.08532743016365174 2307 56-57 0.07093997059203616 2307 58-59 0.19014778909043173 2307 60-61 0.08727494453051321 2307 62-63 0.18752687444745675 2307 64-65 0.20145311844205338 2307 66-67 -0.03386770216643953 2307 68-69 0.006970422886652727 2307 70-71 -0.11455960133414322 2307 72-73 -0.2093628711524147 2307 74-75 -0.19118234150740676 2307 76-77 -0.096986198362643 2307 78-79 0.03805697678104991 2307 80-81 0.08405868454296694 2307 82-83 -0.09628383812082575 2307 84-85 -0.20467402444216987 2307 86-87 -0.1035104414324195 2307 88-89 0.1373819381765351 2307 90-91 0.19366241267198703 2307 92-93 0.06277943493919125 2307 94-95 -0.006381976578627047 2307 96-97 0.10263109290278294 2307 98-99 0.041784346616754675 2307 100-101 -0.02479942072944752 2201 1 -0.026353584190992763 2201 2 0.03600983080150755 2201 3 -0.017033539821312615 2201 4 0.10184209863083993 2201 5 -0.04732155236406754 2201 6 -0.02351248995375954 2201 7 0.25137100801700996 2201 8 -0.01163741935813789 2201 9 -0.018923873906480537 2201 10-11 0.02261014769912606 2201 12-13 0.06788775589501483 2201 14-15 0.2627168175516772 2201 16-17 0.23735458277506893 2201 18-19 0.17039892638627663 2201 20-21 0.26109636669374225 2201 22-23 0.07820400426551544 2201 24-25 0.15139424984893424 2201 26-27 0.05084097251755537 2201 28-29 0.06819306809472181 2201 30-31 0.1637284656995348 2201 32-33 0.10099799401830722 2201 34-35 0.058635175666289285 2201 36-37 0.0439353428652538 2201 38-39 0.10714982323997901 2201 40-41 0.21643917730024498 2201 42-43 0.21247359551423273 2201 44-45 0.15405856951150554 2201 46-47 0.07186615357005621 2201 48-49 0.024105762670750153 2201 50-51 -0.002952431573625347 2201 52-53 0.09029561505819572 2201 54-55 0.22437344061506082 2201 56-57 0.24571561636092554 2201 58-59 0.2400591285164353 2201 60-61 0.0403475363170287 2201 62-63 -0.0376893713953379 2201 64-65 -0.016489836609117958 2201 66-67 0.05417078565015743 2201 68-69 -0.006091614702590675 2201 70-71 0.06070996934663242 2201 72-73 0.025107903646187424 2201 74-75 0.055144735812937995 2201 76-77 0.08611394865174304 2201 78-79 0.05216933866152118 2201 80-81 0.11800835139011667 2201 82-83 0.03103214798768761 2201 84-85 0.12054855746362847 2201 86-87 0.07786589767012941 2201 88-89 0.144676939953289 2201 90-91 0.0279589678918839 2201 92-93 0.041495247891521814 2201 94-95 0.02874551121226787 2201 96-97 -0.024120296821806164 2201 98-99 0.013104024478909793 2201 100-101 -0.15902171551854494 1101 1 0.3152655269499327 1101 2 0.24489175598925073 1101 3 0.2705173959541547 1101 4 0.41309232907843807 1101 5 0.3983535631339521 1101 6 0.40555795297391484 1101 7 0.47307889791009217 1101 8 0.30435001223003866 1101 9 0.41130397496377213 1101 10-11 0.33947591687521594 1101 12-13 0.3428929554679101 1101 14-15 0.5760423420335385 1101 16-17 0.5327962131923769 1101 18-19 0.5272000521096274 1101 20-21 0.576760749767061 1101 22-23 0.35547718792709304 1101 24-25 0.4647078564456635 1101 26-27 0.46742642881645935 1101 28-29 0.5172708980018186 1101 30-31 0.543081816017434 1101 32-33 0.36405967972617503 1101 34-35 0.35516766171181047 1101 36-37 0.3503568259395422 1101 38-39 0.39259498472626575 1101 40-41 0.33196841366544305 1101 42-43 0.4133869906686911 1101 44-45 0.3711199607526794 1101 46-47 0.36234656879339155 1101 48-49 0.48546217831088967 1101 50-51 0.409794727434587 1101 52-53 0.43787873123289955 1101 54-55 0.5831038926467329 1101 56-57 0.39874906617312433 1101 58-59 0.5101255775815048 1101 60-61 0.47226793581297954 1101 62-63 0.42586742333902805 1101 64-65 0.42694587663095973 1101 66-67 0.48423125678840506 1101 68-69 0.46089267942321754 1101 70-71 0.4778375312173253 1101 72-73 0.39414120917814444 1101 74-75 0.3021283507247645 1101 76-77 0.2783717951552873 1101 78-79 0.3956026050437629 1101 80-81 0.3155323211047616 1101 82-83 0.37024595569081953 1101 84-85 0.4777097509733821 1101 86-87 0.4809726477493399 1101 88-89 0.5420452810722729 1101 90-91 0.6406183142349029 1101 92-93 0.5113347719861103 1101 94-95 0.5424988561302087 1101 96-97 0.509832808725939 1101 98-99 0.370812807896975 1101 100-101 0.2651102933053302 2304 1 0.2129243978395685 2304 2 0.19833813332024874 2304 3 0.2557188844444056 2304 4 0.34176118859213034 2304 5 0.321050306023821 2304 6 0.3021803003731236 2304 7 0.4170217570006045 2304 8 0.2572657259801474 2304 9 0.2899446730301989 2304 10-11 0.4266695957523794 2304 12-13 0.4258029390637432 2304 14-15 0.5306383779681596 2304 16-17 0.41799317965275407 2304 18-19 0.4450746744442071 2304 20-21 0.6162319118713739 2304 22-23 0.4192650595054914 2304 24-25 0.4066007849020892 2304 26-27 0.3314605485442925 2304 28-29 0.30520414811631014 2304 30-31 0.35973147445231746 2304 32-33 0.3190697007468586 2304 34-35 0.29867004472031766 2304 36-37 0.37558671547714084 2304 38-39 0.3414806924458773 2304 40-41 0.21666171373824028 2304 42-43 0.2787192535633096 2304 44-45 0.33383953651878073 2304 46-47 0.3151583433492746 2304 48-49 0.21308083013180834 2304 50-51 0.16155199162000855 2304 52-53 0.24675480095450553 2304 54-55 0.5083670562250902 2304 56-57 0.5066210765292496 2304 58-59 0.45465765041588213 2304 60-61 0.4944628346258071 2304 62-63 0.4773829614048992 2304 64-65 0.35721575407382744 2304 66-67 0.35493958889843213 2304 68-69 0.22063082014676638 2304 70-71 0.44483793381296977 2304 72-73 0.2345134701019731 2304 74-75 0.397893891574725 2304 76-77 0.3633592971421109 2304 78-79 0.3995878126066188 2304 80-81 0.3875703662244945 2304 82-83 0.2829925455234701 2304 84-85 0.40693584943011984 2304 86-87 0.4460897430133173 2304 88-89 0.5734505254795543 2304 90-91 0.3517325693941551 2304 92-93 0.33033748891615033 2304 94-95 0.36030281331131064 2304 96-97 0.17751237633888906 2304 98-99 0.37261023040743524 2304 100-101 0.19442084235737767 2202 1 -0.02327290906697499 2202 2 -0.08430057556016024 2202 3 -0.0820303395256552 2202 4 -0.0033230982208252158 2202 5 0.0071563173905957456 2202 6 -0.0348826896218597 2202 7 0.2551420900204562 2202 8 -0.11028918880473526 2202 9 -0.1155240619071094 2202 10-11 0.10724274750862861 2202 12-13 0.07524513295590651 2202 14-15 0.21317311633163882 2202 16-17 0.11359142786380261 2202 18-19 0.1694306249353872 2202 20-21 0.16884634906310936 2202 22-23 0.06276072537738742 2202 24-25 0.051696782679862 2202 26-27 0.0334369880825065 2202 28-29 0.08904337370733373 2202 30-31 0.07183489940966581 2202 32-33 0.03553860206555726 2202 34-35 -0.1582302485536573 2202 36-37 -0.09225433245426018 2202 38-39 -0.01608227005689855 2202 40-41 -0.03594122671365341 2202 42-43 -0.09584467366590133 2202 44-45 0.029210349224044307 2202 46-47 -0.011907767189377694 2202 48-49 -0.0203321948889581 2202 50-51 0.060772998234561726 2202 52-53 -0.02057324959116613 2202 54-55 0.20472201842509463 2202 56-57 0.21697678280396815 2202 58-59 -0.0021046105715427643 2202 60-61 0.2677980388571797 2202 62-63 0.20190279399523092 2202 64-65 0.13259359335797427 2202 66-67 0.1291598278735222 2202 68-69 0.11061258960214104 2202 70-71 0.21282087008033557 2202 72-73 0.0844231742787187 2202 74-75 0.16556448784135824 2202 76-77 0.19277538415172302 2202 78-79 0.23864195225416296 2202 80-81 0.17573864993261168 2202 82-83 0.07548760228237938 2202 84-85 0.1439703475045917 2202 86-87 0.1982588779086356 2202 88-89 0.1438876229597632 2202 90-91 0.22210425685135604 2202 92-93 0.21853247933672293 2202 94-95 0.24127727983758618 2202 96-97 0.22240343223232628 2202 98-99 0.19809307562918477 2202 100-101 0.06821748716595621 1102 1 -0.02869163103067507 1102 2 0.005071839607047934 1102 3 0.047834639330158524 1102 4 0.12335209688109927 1102 5 0.04744702903079201 1102 6 0.03227780696397531 1102 7 0.12606813284511986 1102 8 0.03028437639775916 1102 9 0.12340470703746576 1102 10-11 0.10995378438386894 1102 12-13 0.07487755367217375 1102 14-15 0.0968761703680272 1102 16-17 0.07367406802899978 1102 18-19 0.106103455165524 1102 20-21 0.0874424345485707 1102 22-23 0.019998629616836183 1102 24-25 -0.11894733763438836 1102 26-27 -0.12682068983685468 1102 28-29 -0.16210805815735796 1102 30-31 0.009600598341009459 1102 32-33 -0.028943072040213735 1102 34-35 -0.11408572695604846 1102 36-37 -0.10073398059113003 1102 38-39 -0.07335201627077481 1102 40-41 -0.11258001768691628 1102 42-43 -0.06364759605154546 1102 44-45 0.01752062040220892 1102 46-47 -0.059675570297969216 1102 48-49 -0.04667835625514982 1102 50-51 -0.1603581553201039 1102 52-53 -0.0946395442094996 1102 54-55 0.022835632824367735 1102 56-57 0.17397894470644104 1102 58-59 0.17390143754149534 1102 60-61 0.0993934707442179 1102 62-63 0.06983047733331205 1102 64-65 0.17925456198710776 1102 66-67 0.12454516231667156 1102 68-69 -0.0806623369844246 1102 70-71 -0.006924133429727419 1102 72-73 -0.0625387103192594 1102 74-75 0.08091585751177632 1102 76-77 0.03419806867224651 1102 78-79 0.03328002445537237 1102 80-81 0.1265279026145123 1102 82-83 0.10771186661173715 1102 84-85 0.12060206248354177 1102 86-87 0.17909894740235188 1102 88-89 0.2250243255836537 1102 90-91 0.10491201139628359 1102 92-93 -0.01068243909382005 1102 94-95 0.17299765888742868 1102 96-97 0.2313772827091185 1102 98-99 -0.02866125888285964 1102 100-101 0.15831500554026334 2305 1 0.09690513425610092 2305 2 0.07341053595086677 2305 3 0.1077243710039788 2305 4 0.17166925335475014 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-0.8520722607936264 1213 80-81 -0.8470824554783292 1213 82-83 -1.075879701276591 1213 84-85 -0.9355338133070248 1213 86-87 -0.884090574188555 1213 88-89 -1.0497163132122473 1213 90-91 -0.8905794513666301 1213 92-93 -0.8526158715985908 1213 94-95 -0.9448845749975554 1213 96-97 -0.9185125754159884 1213 98-99 -0.8131652908392972 1213 100-101 -0.6528560378210209 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 394.0 3 167.0 4 225.0 5 209.0 6 735.0 7 2999.0 8 11291.0 9 32992.0 10 44572.0 11 33806.0 12 20553.0 13 14355.0 14 11321.0 15 9723.0 16 9284.0 17 9681.0 18 10295.0 19 11096.0 20 12023.0 21 13386.0 22 14912.0 23 16497.0 24 18303.0 25 21225.0 26 24547.0 27 29107.0 28 35608.0 29 43751.0 30 52472.0 31 66175.0 32 88064.0 33 122084.0 34 182906.0 35 293826.0 36 524420.0 37 941706.0 38 493094.0 39 1723.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.231571907936484 25.664319364974446 20.92549596589613 16.178612761192937 2 30.87461288027036 26.432642489464957 29.15692611765702 13.535818512607664 3 35.97788778652028 22.171130440408948 25.85319162570372 15.997790147367057 4 29.6661827744103 21.51856525686778 30.84298344304634 17.97226852567558 5 32.890305819320425 19.782479266038607 30.146224897924395 17.18099001671657 6 29.624883917225286 20.28109914293125 33.313874760603575 16.780142179239892 7 33.578518553526386 21.083617726216712 26.91694691388119 18.420916806375708 8 28.81273627138878 29.51558147632312 24.158158824114604 17.513523428173496 9 30.763374180469167 26.79710765345189 24.3625387712035 18.07697939487544 10-11 26.936161992477764 29.922320558127012 27.747628766772227 15.393888682622995 12-13 27.811397190891686 27.10491541882089 27.5085659943354 17.575121395952024 14-15 25.684997359699956 27.296458671337597 28.605944895531326 18.41259907343112 16-17 26.097075480907034 27.69680365240023 28.889426356049334 17.316694510643405 18-19 25.56097165193979 27.852529095607924 29.318994053657743 17.267505198794534 20-21 24.503611924908768 29.1060204136667 28.773201393198793 17.61716626822574 22-23 26.019153373744814 28.226850043902086 28.049142976536633 17.704853605816467 24-25 23.362454747718235 30.48636433269986 28.249014769333876 17.90216615024803 26-27 22.68225419421498 30.99254612705895 28.217111901853524 18.108087776872544 28-29 23.35000556674087 30.354380589676467 28.096631779683946 18.198982063898722 30-31 24.606994723401183 28.541623924732274 28.562124487135705 18.289256864730845 32-33 24.514153656526062 28.53528441065799 28.661500914470178 18.28906101834577 34-35 22.457983474553448 28.540567738793698 28.348875174248278 20.65257361240458 36-37 21.043309982773156 28.496438254647472 28.761076206793746 21.699175555785626 38-39 20.78377269439576 29.55337226711554 29.807357970979513 19.855497067509184 40-41 21.630959456704993 28.602231174436493 30.95884941744774 18.80795995141077 42-43 22.45611761612773 28.403041955384325 30.331412148632538 18.809428279855407 44-45 21.464921843771005 29.411613566689926 30.24502255358569 18.878442035953373 46-47 22.595591882759624 30.03832163540947 28.36649843357103 18.999588048259874 48-49 20.796155620205077 29.594290175989475 29.750418662913518 19.859135540891927 50-51 20.42735818803642 28.519229124910467 30.567073418596063 20.48633926845705 52-53 23.34295267697212 28.466814510407907 28.398862873035096 19.791369939584875 54-55 23.735523858238718 28.414524295004597 28.72916700728177 19.120784839474915 56-57 24.056184102558273 28.361983272096523 28.426923929261715 19.154908696083485 58-59 22.959645026343512 28.70200883385486 28.311453724256975 20.026892415544655 60-61 21.076349071475654 28.521326184906542 28.232227764096933 22.170096979520874 62-63 22.489438254789814 28.580357294356336 28.379668957645766 20.550535493208088 64-65 20.931192983308208 29.468215733633706 29.798197259151472 19.802394023906615 66-67 20.31743481557903 29.352920379442192 28.909574621280935 21.420070183697835 68-69 20.141274374508026 28.765173349209356 31.399102365460685 19.69444991082193 70-71 20.058941625468186 30.57140717838139 29.851915859677714 19.517735336472715 72-73 19.918873379744028 32.36217463547889 28.149038255746532 19.56991372903055 74-75 19.84244574907697 32.44347010388942 28.244586738431487 19.469497408602127 76-77 19.969202423083114 32.3694924657707 28.066549167621694 19.594755943524493 78-79 19.88618443563751 32.33698012648733 28.196314724507243 19.58052071336791 80-81 19.82511042302086 32.037863057861806 28.25784691962157 19.87917959949577 82-83 19.948330915724732 31.89214273312015 28.087550911878363 20.071975439276756 84-85 19.95533549607278 31.572622172615755 28.343601518630507 20.128440812680957 86-87 20.11226839776958 31.22836575518015 28.377418013705775 20.281947833344493 88-89 20.191852093950413 31.33161191914533 28.272850013834738 20.203685973069515 90-91 20.31329162505648 30.95192275654529 28.275776596344283 20.459009022053944 92-93 20.295154990070387 31.00343107908231 28.203635057216303 20.497778873631 94-95 20.220210590873254 30.914830475825788 28.348202646044978 20.516756287255973 96-97 20.375269354787495 30.733046602658654 28.268633187498327 20.623050855055528 98-99 20.35669652942192 30.776682296310586 28.215703098107113 20.65091807616038 100-101 20.54775047348455 30.730439840836944 28.08602627348817 20.635783412190335 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 80.0 1 123.5 2 195.0 3 220.0 4 209.0 5 248.5 6 348.0 7 472.5 8 650.0 9 832.5 10 1032.5 11 1187.0 12 1235.5 13 1269.5 14 1244.0 15 1213.5 16 1214.5 17 1257.0 18 1518.5 19 1945.0 20 2652.5 21 3968.0 22 5680.0 23 7909.0 24 11008.5 25 15059.5 26 20210.0 27 26317.0 28 33184.5 29 40739.5 30 49236.5 31 58303.0 32 68217.0 33 79559.5 34 91402.0 35 103713.0 36 117064.0 37 130178.0 38 140909.0 39 148172.0 40 151678.0 41 151913.0 42 149925.5 43 147386.0 44 143903.0 45 138974.0 46 134115.5 47 128652.0 48 120690.0 49 111384.0 50 102270.5 51 92679.0 52 82813.5 53 72513.5 54 61969.0 55 52163.5 56 43129.0 57 35318.0 58 28803.5 59 23027.5 60 18138.5 61 14129.0 62 10795.0 63 8117.5 64 6071.5 65 4511.0 66 3327.0 67 2472.0 68 1859.5 69 1452.5 70 1088.0 71 794.5 72 619.5 73 479.5 74 396.0 75 351.0 76 292.0 77 220.5 78 174.5 79 158.0 80 127.0 81 109.0 82 99.0 83 76.5 84 65.0 85 62.0 86 53.5 87 43.5 88 38.5 89 36.5 90 32.0 91 25.0 92 19.0 93 15.5 94 10.5 95 6.0 96 6.5 97 4.5 98 2.0 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1879158025386959 2 0.17791433337878515 3 0.15011521878834996 4 0.14362358197337685 5 0.14763038172998705 6 0.128869861939347 7 0.10308967745883167 8 0.08967155734367191 9 0.0900753433656559 10-11 0.07039853990974451 12-13 0.08956284572236853 14-15 0.09431509659648762 16-17 0.08675187380009548 18-19 0.08310226937062494 20-21 0.08117652065039367 22-23 0.10184725892965023 24-25 0.44851308903450726 26-27 0.285212703605219 28-29 0.12427291338137558 30-31 0.23046863716316093 32-33 0.2602245609370569 34-35 0.24457008746937048 36-37 0.24753636170779122 38-39 0.2646196164840363 40-41 0.2648681001898726 42-43 0.29057063351231405 44-45 0.2676946023437604 46-47 0.28574073148012114 48-49 0.27053663472926304 50-51 0.2849952803626123 52-53 0.28437407109802154 54-55 0.26166887247723036 56-57 0.2765468343641784 58-59 0.2666385465939562 60-61 0.24283070152851643 62-63 0.23548490197473104 64-65 0.23385422765518038 66-67 0.242659868980754 68-69 0.2477071942555537 70-71 0.23405612066617237 72-73 0.225638735130968 74-75 0.23463073923591882 76-77 0.235624674059264 78-79 0.23804739019116783 80-81 0.24539318974495322 82-83 0.27052110449764827 84-85 0.2632684863335515 86-87 0.26314424448063334 88-89 0.2611408446023282 90-91 0.25388822643823145 92-93 0.23040651623670183 94-95 0.229055386086217 96-97 0.22428760498048317 98-99 0.24853029653113642 100-101 0.25559655191585595 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3219527.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 94.19263902393871 #Duplication Level Percentage of deduplicated Percentage of total 1 98.0994254545891 92.40243770299895 2 1.2728542009975325 2.39786992569329 3 0.19913169835557515 0.5627022054429163 4 0.1295970871381118 0.4882836658941638 5 0.06654292533806627 0.313392687298269 6 0.046638112318236793 0.2635780127009753 7 0.032401462262140496 0.21363854670939006 8 0.03339598989724568 0.2516525136990693 9 0.01486114121005605 0.12598290985343302 >10 0.09049956613103939 1.8027072529646988 >50 0.012202224556041665 0.8033346987623807 >100 0.002450137206884652 0.37441987798243886 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0018015068673131178 0.0 0.0 2 0.0019257487202312639 0.0 3.1060463229536514E-5 3 0.0024537765951333844 0.0 3.1060463229536514E-5 4 0.0027643812274287497 0.0 6.212092645907303E-5 5 0.003478771881708089 0.0 9.318138968860953E-5 6 0.004441646241823721 0.0 9.318138968860953E-5 7 0.005218157822562134 0.0 9.318138968860953E-5 8 0.005932548476841474 0.0 9.318138968860953E-5 9 0.006491636814973131 0.0 1.5530231614768256E-4 10-11 0.009721924990844929 0.0 1.5530231614768256E-4 12-13 0.012331003902125996 0.0 2.1742324260675558E-4 14-15 0.015064324666325207 0.0 2.484837058362921E-4 16-17 0.017518101261458592 0.0 2.484837058362921E-4 18-19 0.02036013364696118 0.0 2.484837058362921E-4 20-21 0.02408738923450557 0.0 2.484837058362921E-4 22-23 0.02842032385502591 0.0 2.484837058362921E-4 24-25 0.03343658866659606 0.0 2.6401393745106036E-4 26-27 0.038887699963379714 0.0 3.106046322953651E-4 28-29 0.04489789959829503 0.0 3.106046322953651E-4 30-31 0.07636214884981551 0.0 3.106046322953651E-4 32-33 0.08495036693278236 0.0 3.106046322953651E-4 34-35 0.09315032922538 0.0 3.261348639101334E-4 36-37 0.10290331467945446 0.0 3.4166509552490163E-4 38-39 0.11346387217749687 0.0 3.571953271396699E-4 40-41 0.12545321098409798 0.0 3.7272555875443814E-4 42-43 0.13921299619478267 0.0 3.7272555875443814E-4 44-45 0.15378035344943528 0.0 3.7272555875443814E-4 46-47 0.16912422228482632 0.0 3.7272555875443814E-4 48-49 0.18626959798753046 0.0 3.882557903692064E-4 50-51 0.20293353651017682 0.0 4.193162535987429E-4 52-53 0.22183382838534976 0.0 4.3484648521351115E-4 54-55 0.24335872940341857 0.0 4.3484648521351115E-4 56-57 0.26646771404619374 0.0 4.3484648521351115E-4 58-59 0.29170434042019217 0.0 4.659069484430477E-4 60-61 0.3197053480216193 0.0 4.659069484430477E-4 62-63 0.3497408159645812 0.0 4.659069484430477E-4 64-65 0.3820902884181434 0.0 4.659069484430477E-4 66-67 0.41622573750740405 0.0 4.659069484430477E-4 68-69 0.4545388189010373 0.0 4.659069484430477E-4 70-71 0.49561628152209936 0.0 4.659069484430477E-4 72-73 0.5410732694585261 0.0 4.659069484430477E-4 74-75 0.5923540942504908 0.0 4.659069484430477E-4 76-77 0.6491636814973131 4.6590694844304774E-5 4.659069484430477E-4 78-79 0.7105546870704921 6.212092645907303E-5 4.8143718005781597E-4 80-81 0.7776918783411352 6.212092645907303E-5 4.969674116725842E-4 82-83 0.8507771483202347 6.212092645907303E-5 4.969674116725842E-4 84-85 0.9335688130585642 6.212092645907303E-5 4.969674116725842E-4 86-87 1.0247312726372537 6.212092645907303E-5 4.969674116725842E-4 88-89 1.1259262618390837 9.318138968860953E-5 4.969674116725842E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 2540 0.0 82.94335 2 GAGCGGC 1580 0.0 77.720825 9 GATCGGG 2775 0.0 77.465454 1 AGAGCGG 1885 0.0 67.91315 8 GAGAGCG 2245 0.0 67.585785 7 GTTCGGA 410 0.0 67.123795 2 TCGGGAG 2525 0.0 66.328224 3 CGGGAGA 2635 0.0 65.71587 4 AAGCGGC 770 0.0 64.65369 9 GATCCGG 555 0.0 61.561947 1 ATCCGGA 545 0.0 60.073776 2 CGGAAAG 1320 0.0 60.020634 5 GGATCGG 930 0.0 57.65917 1 TCGGGAA 1125 0.0 55.66811 3 AAAGCGG 1000 0.0 55.47372 8 GGTTCGG 595 0.0 55.030697 1 TTCGGAA 585 0.0 53.527027 3 ATTCGGA 570 0.0 52.444267 2 TCGGAAA 1150 0.0 52.39187 4 TTCGGAG 505 0.0 51.67213 3 >>END_MODULE