##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n02_50330665-2.34200000035755.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2529953 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.056155193396876 33.0 33.0 33.0 33.0 33.0 2 32.00397517266131 33.0 33.0 33.0 33.0 33.0 3 31.986581173642357 33.0 33.0 33.0 33.0 33.0 4 35.740833525365886 37.0 37.0 37.0 33.0 37.0 5 35.74857991433043 37.0 37.0 37.0 33.0 37.0 6 35.77221118336981 37.0 37.0 37.0 33.0 37.0 7 35.69353146086113 37.0 37.0 37.0 33.0 37.0 8 35.657123274622094 37.0 37.0 37.0 33.0 37.0 9 35.61181887568662 37.0 37.0 37.0 33.0 37.0 10-11 35.535010531816205 37.0 37.0 37.0 33.0 37.0 12-13 35.53088140372569 37.0 37.0 37.0 33.0 37.0 14-15 37.27042063627269 40.0 37.0 40.0 33.0 40.0 16-17 37.24294305072071 40.0 37.0 40.0 33.0 40.0 18-19 37.24210805497177 40.0 37.0 40.0 33.0 40.0 20-21 37.18324885877327 40.0 37.0 40.0 33.0 40.0 22-23 37.255871749396135 40.0 37.0 40.0 33.0 40.0 24-25 37.05744612646954 40.0 37.0 40.0 33.0 40.0 26-27 37.012989767003575 40.0 37.0 40.0 33.0 40.0 28-29 37.0169485361981 40.0 37.0 40.0 33.0 40.0 30-31 36.992644329756324 40.0 37.0 40.0 33.0 40.0 32-33 36.91221536526568 40.0 37.0 40.0 33.0 40.0 34-35 36.860328828243055 40.0 37.0 40.0 33.0 40.0 36-37 36.78331000615427 40.0 37.0 40.0 33.0 40.0 38-39 36.74086317018538 40.0 37.0 40.0 33.0 40.0 40-41 36.677620690977264 40.0 37.0 40.0 33.0 40.0 42-43 36.578807985761 40.0 37.0 40.0 33.0 40.0 44-45 36.45085402772304 40.0 37.0 40.0 33.0 40.0 46-47 36.38297628453967 40.0 37.0 40.0 33.0 40.0 48-49 36.27806010625494 38.5 37.0 40.0 33.0 40.0 50-51 35.11719941042383 37.0 35.0 38.5 30.0 38.5 52-53 35.52707619469611 37.0 37.0 38.5 30.0 40.0 54-55 36.342894117005336 37.0 37.0 40.0 33.0 40.0 56-57 36.384436390715564 38.5 37.0 40.0 33.0 40.0 58-59 36.21517356251282 37.0 37.0 40.0 33.0 40.0 60-61 35.98001978692885 37.0 37.0 40.0 30.0 40.0 62-63 35.79146411020284 37.0 37.0 40.0 27.0 40.0 64-65 35.578330111270844 37.0 37.0 40.0 27.0 40.0 66-67 35.349178818736945 37.0 37.0 40.0 27.0 40.0 68-69 35.144316317338706 37.0 35.0 38.5 27.0 40.0 70-71 34.82528390843625 37.0 33.0 37.0 27.0 40.0 72-73 34.59189755699019 37.0 33.0 37.0 27.0 40.0 74-75 34.364802033871776 37.0 33.0 37.0 27.0 40.0 76-77 34.12968343680693 37.0 33.0 37.0 27.0 37.0 78-79 33.90217268858355 37.0 33.0 37.0 27.0 37.0 80-81 33.67903870150948 37.0 33.0 37.0 27.0 37.0 82-83 33.47387026557411 37.0 33.0 37.0 27.0 37.0 84-85 33.29139217210755 37.0 33.0 37.0 27.0 37.0 86-87 33.14137752756672 37.0 33.0 37.0 27.0 37.0 88-89 32.9971278122558 37.0 33.0 37.0 27.0 37.0 90-91 32.84767187374627 37.0 33.0 37.0 27.0 37.0 92-93 32.70511527289242 37.0 33.0 37.0 27.0 37.0 94-95 32.591810203588764 35.0 33.0 37.0 27.0 37.0 96-97 32.461639010685175 33.0 33.0 37.0 24.5 37.0 98-99 32.32840610082479 33.0 33.0 37.0 22.0 37.0 100-101 31.12273073847617 33.0 30.0 37.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.33261832687525583 2306 2 0.2923873913395312 2306 3 0.23467369056972132 2306 4 0.37204598298503555 2306 5 0.33304096211472967 2306 6 0.37677744855115236 2306 7 0.36380318014460755 2306 8 0.39922411951734205 2306 9 0.35193242631842026 2306 10-11 0.3038825636589664 2306 12-13 0.407768715758948 2306 14-15 0.5679327001789289 2306 16-17 0.5149249765645152 2306 18-19 0.49425424821387054 2306 20-21 0.6899758050915921 2306 22-23 0.6196161093070174 2306 24-25 0.3517709400293185 2306 26-27 0.5270717944933594 2306 28-29 0.39698580847895215 2306 30-31 0.32996650308240305 2306 32-33 0.25524048284539447 2306 34-35 0.30082819379359904 2306 36-37 0.2639021467687357 2306 38-39 0.22588841204538568 2306 40-41 0.15523406984812027 2306 42-43 0.169242730666042 2306 44-45 0.22018386130412892 2306 46-47 0.24230003819513968 2306 48-49 0.23575311361718576 2306 50-51 0.13355045297809198 2306 52-53 0.17623963524892616 2306 54-55 0.30352648915226865 2306 56-57 0.31605845617789186 2306 58-59 0.3262233482686199 2306 60-61 0.4452221866928028 2306 62-63 0.3467945733367017 2306 64-65 0.4257226738977806 2306 66-67 0.361963188387044 2306 68-69 0.12404339153564337 2306 70-71 0.18387273919574199 2306 72-73 0.19104076775014533 2306 74-75 0.2745369845401555 2306 76-77 0.18923907470399115 2306 78-79 0.3614903457676846 2306 80-81 0.05401293923552686 2306 82-83 0.20896404001756963 2306 84-85 0.14052138135624404 2306 86-87 0.24181759203198538 2306 88-89 0.28723738938609955 2306 90-91 0.2785195001476026 2306 92-93 0.36730366110387536 2306 94-95 0.26698745205415975 2306 96-97 0.23840986049835067 2306 98-99 0.26237074145745254 2306 100-101 0.081899042226528 2307 1 0.22403154332643282 2307 2 0.19725966514445048 2307 3 0.19037322303905313 2307 4 0.32436038478739704 2307 5 0.2741159277928418 2307 6 0.21036973255157676 2307 7 0.3338433851286169 2307 8 0.2848246006564281 2307 9 0.23776526875123238 2307 10-11 0.30236882086162353 2307 12-13 0.31396973821036056 2307 14-15 0.4092444398552786 2307 16-17 0.39062294761532 2307 18-19 0.5306939300918216 2307 20-21 0.6540670363688434 2307 22-23 0.4871498055518728 2307 24-25 0.4329619258923074 2307 26-27 0.35508879605755084 2307 28-29 0.31155600334459876 2307 30-31 0.318228091909063 2307 32-33 0.32114299046502737 2307 34-35 0.2023779788208273 2307 36-37 0.1675712642864582 2307 38-39 0.1391770711170608 2307 40-41 0.21696410305449376 2307 42-43 0.1915062135612544 2307 44-45 0.19930035145747382 2307 46-47 0.1229353010118146 2307 48-49 0.1178925532284083 2307 50-51 -0.07049578105633003 2307 52-53 -0.05278497499045898 2307 54-55 0.02662526924004993 2307 56-57 0.11458995131635419 2307 58-59 0.13778667073271578 2307 60-61 0.11620708074006103 2307 62-63 0.14837577188279027 2307 64-65 0.17000660150103641 2307 66-67 -0.026569272609116013 2307 68-69 0.17571199557503547 2307 70-71 0.12307369406797619 2307 72-73 0.12128840687608289 2307 74-75 0.22085905704243203 2307 76-77 0.33968560645710255 2307 78-79 0.17443796141062506 2307 80-81 0.20566339872766548 2307 82-83 -0.022568868729244684 2307 84-85 -0.05944402674332139 2307 86-87 0.11743610354344014 2307 88-89 0.2608920005234623 2307 90-91 0.2534507097871739 2307 92-93 0.22831858931410665 2307 94-95 0.08825625580699636 2307 96-97 0.24823445395094268 2307 98-99 0.10083057797755401 2307 100-101 -0.031777471971423665 2201 1 0.20975507434442875 2201 2 0.33884611625936856 2201 3 0.25257691643037816 2201 4 0.3603203160711388 2201 5 0.3139631867752968 2201 6 0.2127821117982336 2201 7 0.43155334490079866 2201 8 0.29635542944414794 2201 9 0.3177521963218055 2201 10-11 0.33822124861388403 2201 12-13 0.3632450569045744 2201 14-15 0.30425541596584793 2201 16-17 0.3734299078239687 2201 18-19 0.4621045269299273 2201 20-21 0.5612818133742721 2201 22-23 0.41800998430873904 2201 24-25 0.4602688979263334 2201 26-27 0.4258830966503311 2201 28-29 0.4479575591357019 2201 30-31 0.38886436231054944 2201 32-33 0.316029047472 2201 34-35 0.43773008513751677 2201 36-37 0.43685901038892183 2201 38-39 0.36826279658313865 2201 40-41 0.21295637140050871 2201 42-43 0.20829826518016148 2201 44-45 0.336701690091445 2201 46-47 0.28469908995227655 2201 48-49 0.32442403778767215 2201 50-51 0.2775759280993171 2201 52-53 0.42428129663655767 2201 54-55 0.46159530800581905 2201 56-57 0.41226077980306997 2201 58-59 0.4543727455852675 2201 60-61 0.45907346890091816 2201 62-63 0.3837031523872412 2201 64-65 0.36757121649863933 2201 66-67 0.43006487782272274 2201 68-69 0.3607545992245633 2201 70-71 0.389619025082105 2201 72-73 0.37504511778403327 2201 74-75 0.28484193387993884 2201 76-77 0.39564656977474755 2201 78-79 0.3229261392472651 2201 80-81 0.3597718611906586 2201 82-83 0.3524002240054571 2201 84-85 0.24760433479535493 2201 86-87 0.39334069200916844 2201 88-89 0.43857061226585614 2201 90-91 0.5321106137650204 2201 92-93 0.35745828155300075 2201 94-95 0.3492393541017762 2201 96-97 0.3948015436798471 2201 98-99 0.570789791137706 2201 100-101 0.1915127381119781 1101 1 0.277760529252042 1101 2 0.1854577378764546 1101 3 0.2611660560524953 1101 4 0.29582377308584284 1101 5 0.2933629909457949 1101 6 0.28338902726756743 1101 7 0.30143201940384756 1101 8 0.25023470151516847 1101 9 0.31120324734546756 1101 10-11 0.23777898231934813 1101 12-13 0.2521572612104279 1101 14-15 0.33534769941945086 1101 16-17 0.43948996700478915 1101 18-19 0.4675762336464473 1101 20-21 0.5074702476741848 1101 22-23 0.3921500204028092 1101 24-25 0.5130290199837972 1101 26-27 0.3231209047038135 1101 28-29 0.2130479502907363 1101 30-31 0.38239910884813355 1101 32-33 0.2686876849690236 1101 34-35 0.3075772613288734 1101 36-37 0.28917317634962814 1101 38-39 0.39670787192709867 1101 40-41 0.24509304887460104 1101 42-43 0.30436286611754326 1101 44-45 0.37018259551491184 1101 46-47 0.3352556801190403 1101 48-49 0.22937278253495208 1101 50-51 0.31188613907200846 1101 52-53 0.43751133750637194 1101 54-55 0.45888855308788123 1101 56-57 0.3953893841098761 1101 58-59 0.4377089387653186 1101 60-61 0.5179280642946011 1101 62-63 0.44788321233603057 1101 64-65 0.38341321736739786 1101 66-67 0.40746363924910867 1101 68-69 0.3779108296506095 1101 70-71 0.4295750834373706 1101 72-73 0.44160540015290195 1101 74-75 0.38395557138897374 1101 76-77 0.41342616678834787 1101 78-79 0.5454734107135195 1101 80-81 0.5804026853784805 1101 82-83 0.4277296363489498 1101 84-85 0.5052977224297734 1101 86-87 0.5880359563429991 1101 88-89 0.4967235452686509 1101 90-91 0.5687827481386165 1101 92-93 0.5364108396958827 1101 94-95 0.5371963796052128 1101 96-97 0.5704607283475482 1101 98-99 0.4928299697841325 1101 100-101 0.3276569907800351 2304 1 0.02707904956313456 2304 2 -0.009477916275187681 2304 3 -0.04612201384749781 2304 4 0.04261336678523975 2304 5 0.03441817038817874 2304 6 0.05668500833271395 2304 7 -0.029135799998876166 2304 8 0.009130089587230827 2304 9 -0.0836932051235948 2304 10-11 0.05887162715899308 2304 12-13 0.034559517692720476 2304 14-15 0.02298050311198807 2304 16-17 0.09800642187416031 2304 18-19 0.07985732889326158 2304 20-21 0.22395981815753174 2304 22-23 0.16964739384089 2304 24-25 -0.0318189099509496 2304 26-27 0.03545015358580628 2304 28-29 -0.0978873024986342 2304 30-31 -0.04178867360563743 2304 32-33 -0.1764729976616195 2304 34-35 -0.17955897951996747 2304 36-37 -0.07058944442795934 2304 38-39 -0.1303147283101751 2304 40-41 -0.1722243558854828 2304 42-43 -0.07586849267081419 2304 44-45 -0.029447570794658873 2304 46-47 -0.03179380548798605 2304 48-49 -0.06551129951608914 2304 50-51 -0.040834442942632165 2304 52-53 -0.05590565723460372 2304 54-55 0.11793991171884954 2304 56-57 0.1218622262466198 2304 58-59 0.1353319002286355 2304 60-61 0.12683434718810815 2304 62-63 0.010983411246449748 2304 64-65 -0.06639571746829631 2304 66-67 -0.06607411831627275 2304 68-69 -0.04865770492458665 2304 70-71 0.13048887121509978 2304 72-73 0.08599609189290192 2304 74-75 0.09975603826772073 2304 76-77 0.11224054952662499 2304 78-79 0.1232924396135715 2304 80-81 0.14356867130325668 2304 82-83 0.04208701895540656 2304 84-85 -0.03297236683398097 2304 86-87 0.16758659783402408 2304 88-89 0.18293411978725516 2304 90-91 -0.021497939843534652 2304 92-93 0.09153359457567944 2304 94-95 0.05082179252632102 2304 96-97 0.04457827240393897 2304 98-99 0.09887716133034274 2304 100-101 0.07126409154754043 2202 1 0.1780134261742603 2202 2 0.07560537193870687 2202 3 0.058135255556607035 2202 4 0.1741822876124388 2202 5 0.23668714432369597 2202 6 0.13962908546074715 2202 7 0.2563075380760438 2202 8 0.1971400281536475 2202 9 0.11599215554402775 2202 10-11 0.17590497414136053 2202 12-13 0.11905156341116196 2202 14-15 0.21889588427904272 2202 16-17 0.21613589621818363 2202 18-19 0.2572986461349629 2202 20-21 0.4124238620103924 2202 22-23 0.34188752368136477 2202 24-25 0.1044107353617747 2202 26-27 0.15755078512852805 2202 28-29 0.22976292258104536 2202 30-31 0.18847278624582486 2202 32-33 0.07774154573938574 2202 34-35 -0.16658819297985872 2202 36-37 0.02756036762291103 2202 38-39 0.01784035576076093 2202 40-41 0.054011285945769316 2202 42-43 -0.02550970421336274 2202 44-45 0.04945613365654111 2202 46-47 0.038525885927420234 2202 48-49 -0.0835312581549914 2202 50-51 0.0025012385419671546 2202 52-53 0.03225416195127906 2202 54-55 0.24030867238013087 2202 56-57 0.07939206167549884 2202 58-59 0.10701047907314631 2202 60-61 0.271027006854311 2202 62-63 0.2059656772059526 2202 64-65 0.06715231322197468 2202 66-67 0.14160498320213577 2202 68-69 0.07429453574862777 2202 70-71 0.1460423007126721 2202 72-73 0.17595454319981485 2202 74-75 0.1442305407733926 2202 76-77 0.021887505306985133 2202 78-79 0.20256292141151988 2202 80-81 0.2497911427874513 2202 82-83 0.12645809930551621 2202 84-85 0.13290382025998326 2202 86-87 0.24904157187175002 2202 88-89 0.10739189414092465 2202 90-91 0.1700280803246912 2202 92-93 0.14806582149635972 2202 94-95 0.22225527095340425 2202 96-97 0.1845662534919441 2202 98-99 0.17606018193261974 2202 100-101 -0.05051601411279805 1102 1 0.11925356453656377 1102 2 0.10863630750007758 1102 3 0.13650522976694646 1102 4 0.1620172375982989 1102 5 0.16533765726159544 1102 6 0.09382708199194667 1102 7 0.2050685628555513 1102 8 0.12961171978393082 1102 9 0.15913383022758865 1102 10-11 0.21018490245820942 1102 12-13 0.07493964892366023 1102 14-15 0.11105029221406681 1102 16-17 0.12193449238814225 1102 18-19 0.18307549212600804 1102 20-21 0.2810162445965787 1102 22-23 0.12629326803578067 1102 24-25 0.02487886078780832 1102 26-27 -0.03584665660886088 1102 28-29 -0.06532500922102713 1102 30-31 -0.08505328484258001 1102 32-33 -0.0975386730764285 1102 34-35 0.07057957389672964 1102 36-37 -0.008882641579660344 1102 38-39 0.014795655399645113 1102 40-41 -0.05259734586523024 1102 42-43 -0.15100964987611576 1102 44-45 0.08416722661068121 1102 46-47 0.14272286065095585 1102 48-49 -0.01887002750510902 1102 50-51 -0.11008506362012582 1102 52-53 0.01907150609553554 1102 54-55 0.09429990087753026 1102 56-57 0.07678501968263873 1102 58-59 0.15583292690795503 1102 60-61 0.31388305287118357 1102 62-63 0.23506924247434569 1102 64-65 0.119183714613051 1102 66-67 0.11509012468621194 1102 68-69 0.08732707182368671 1102 70-71 -0.030358850664036652 1102 72-73 0.21134337157604222 1102 74-75 0.243110582272152 1102 76-77 0.14408204640934485 1102 78-79 0.2544366588344431 1102 80-81 0.09547326052299354 1102 82-83 0.042057645431924584 1102 84-85 0.25290281182176955 1102 86-87 0.2623483725843059 1102 88-89 0.3247423220300405 1102 90-91 0.220506238565072 1102 92-93 0.23884101827073323 1102 94-95 0.3324502566853411 1102 96-97 0.1854282007823258 1102 98-99 0.07648846258937425 1102 100-101 0.00979556273888349 2305 1 0.08125005110255756 2305 2 0.031132387493009617 2305 3 0.11372551442108403 2305 4 0.062246070628475536 2305 5 0.16671472373736407 2305 6 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-1.0836616014393137 1213 100-101 -0.9797823514249053 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 258.0 3 112.0 4 76.0 5 122.0 6 438.0 7 1704.0 8 6154.0 9 10763.0 10 10179.0 11 9764.0 12 7273.0 13 5662.0 14 4826.0 15 4451.0 16 4607.0 17 4995.0 18 5445.0 19 6062.0 20 6832.0 21 7672.0 22 8949.0 23 9658.0 24 11245.0 25 12910.0 26 15350.0 27 18230.0 28 21855.0 29 27006.0 30 34136.0 31 43775.0 32 59861.0 33 86034.0 34 131803.0 35 225453.0 36 456821.0 37 902698.0 38 365944.0 39 830.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.8689242859072 24.810499933272535 26.24852635259744 13.07204942822283 2 31.668406272718492 22.213277813766595 35.14109815337782 10.9772177601371 3 39.92111399953368 18.416524257801413 26.819458810685077 14.842902931979825 4 30.73189770270864 17.845817815061654 36.89793427047927 14.524350211750436 5 30.502508067299804 16.658697008369725 38.21695257156067 14.621842352769798 6 23.491992755802798 17.395888411500795 44.53546743752354 14.576651395172856 7 33.90007889674339 17.17183267559669 32.67231262419115 16.255775803468765 8 30.074665630666043 28.053950101852738 26.589201355082487 15.282182912398737 9 30.741004635507185 27.768733411059937 24.83495624218893 16.655305711243944 10-11 24.723751442750018 28.816019410179265 30.589207636548032 15.87102151052268 12-13 25.829495307232975 26.38886498673777 29.88558815017725 17.896051555852008 14-15 23.792582909686022 27.30698784140955 31.172779854994225 17.7276493939102 16-17 21.990030246320288 28.875019261234964 32.50869203406751 16.626258458377237 18-19 21.099716675330182 29.377745919246536 32.311394462153885 17.211142943269397 20-21 21.133529199313745 29.563249455382074 31.756298362407975 17.546922982896206 22-23 21.18679275966322 29.062065625493354 32.02504133791206 17.726100276931366 24-25 20.86260108466872 29.54022878973952 31.561723637781476 18.03544648781028 26-27 20.493674370980024 29.946609387923615 31.19574000910556 18.363976231990804 28-29 20.28987973453744 29.74211586483096 30.935708038671738 19.03229636195986 30-31 20.186537171751507 30.039785775604738 30.646733767280743 19.12694328536301 32-33 19.57443731034394 30.122420107926253 31.897803927707045 18.405338654022767 34-35 19.9046794172837 30.175791155289584 31.650550100162288 18.26897932726443 36-37 20.03536253532539 30.635346884679503 31.561746255483918 17.76754432451119 38-39 20.793771882721494 30.07701925382273 30.799835142734295 18.329373720721488 40-41 20.19825243649538 29.690171867389974 30.682008671636947 19.4295670244777 42-43 20.370567832722227 30.183093846001384 31.22756912099891 18.218769200277475 44-45 19.911529763493505 30.481353146005812 31.41606869798673 18.191048392513952 46-47 20.27479558689259 30.05503403136121 31.189346676742396 18.4808237050038 48-49 20.13025897630902 30.52622755848437 30.490539896269976 18.852973568936633 50-51 19.953974076132237 30.12204876401448 30.431887419607236 19.492089740246048 52-53 20.338922924444898 29.933270139700475 30.29683501368546 19.430971922169174 54-55 20.840369634405587 29.950202504794394 30.059157435019845 19.150270425780175 56-57 20.905238526677746 30.270590230960632 29.869914716497153 18.95425652586447 58-59 20.723226841549096 30.1975155065356 30.438760206335058 18.640497445580248 60-61 20.179408441848263 30.63434208485351 30.041722110261983 19.144527363036243 62-63 20.443496269532584 30.58210742438784 29.962076304970292 19.012320001109284 64-65 20.129066992896345 30.33767623949516 30.259473027617144 19.27378373999136 66-67 20.429398193546547 29.553465974577158 30.254648366786835 19.762487465089457 68-69 20.337337043044965 29.697510842665064 30.89093701388289 19.07421510040708 70-71 19.642194271352682 30.80664260351138 30.567952266686632 18.9832108584493 72-73 19.536275355670647 31.3571300110205 30.061547639703278 19.045046993605574 74-75 20.050503559871064 30.581395095473635 29.93544236273306 19.432658981922238 76-77 20.07389406510062 30.348267181796356 30.146278713580866 19.431560039522154 78-79 19.887338217311612 30.54948024120247 30.469570681233947 19.093610860251967 80-81 19.17865615388806 30.848196995735787 30.30253624085621 19.670610609519944 82-83 18.665274304578546 30.70481395321188 30.301550753183342 20.32836098902623 84-85 18.672347818368003 30.38027178858 30.828569979298038 20.11881041375397 86-87 18.82718153119915 30.282761506740417 31.452259104062165 19.43779785799827 88-89 18.990273494921965 30.812032104262965 30.891109864588994 19.306584536226072 90-91 19.2538315582466 30.48659705006123 30.78877488207199 19.47079650962018 92-93 18.67099608343998 31.577282486125256 30.592843943910374 19.158877486524382 94-95 18.450536503894536 31.837998245854504 30.515614984871085 19.19585026537987 96-97 19.009342525395393 31.753217416484432 30.344361655498115 18.893078402622063 98-99 19.305604230388322 30.87179448573193 29.998480486735563 19.824120797144186 100-101 19.169563980374086 30.92179353162662 29.568252046083337 20.34039044191596 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 133.0 1 141.5 2 165.5 3 234.5 4 267.5 5 280.0 6 387.5 7 538.0 8 708.5 9 887.5 10 1044.0 11 1121.0 12 1106.5 13 1046.0 14 976.0 15 942.5 16 942.5 17 948.5 18 1028.5 19 1265.5 20 1647.0 21 2318.0 22 3280.5 23 4580.0 24 6409.5 25 9164.0 26 13190.5 27 18580.5 28 24450.5 29 30181.0 30 36938.5 31 43990.0 32 52314.0 33 64619.5 34 80367.5 35 100033.0 36 127442.5 37 156279.5 38 182244.0 39 194399.5 40 186349.0 41 166705.0 42 147512.5 43 137894.5 44 124388.5 45 108084.5 46 101327.0 47 90904.0 48 70453.0 49 52486.0 50 40138.5 51 32314.0 52 26617.5 53 20599.0 54 15348.5 55 11225.5 56 7964.5 57 5728.5 58 4267.0 59 3230.0 60 2324.0 61 1716.0 62 1373.5 63 1047.5 64 751.5 65 539.5 66 447.5 67 373.0 68 275.0 69 208.0 70 169.0 71 140.0 72 106.0 73 88.0 74 69.0 75 53.0 76 47.0 77 37.5 78 26.0 79 17.5 80 15.0 81 13.0 82 11.5 83 9.5 84 7.5 85 4.0 86 4.0 87 3.5 88 1.5 89 0.5 90 0.0 91 1.0 92 1.5 93 2.0 94 2.5 95 2.0 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18798768198460603 2 0.1769598091347942 3 0.14925178451931717 4 0.1434809263255088 5 0.14644540827438296 6 0.12810514661734823 7 0.10272918113498553 8 0.09027835694971409 9 0.09051551550562402 10-11 0.07094993464305463 12-13 0.09037717301467656 14-15 0.09480413272499529 16-17 0.08725458536186245 18-19 0.08344028525431105 20-21 0.08039675045346692 22-23 0.10324302467279037 24-25 0.4504431505249307 26-27 0.2892741485711395 28-29 0.12027891427232047 30-31 0.2246682052986755 32-33 0.2557557393358691 34-35 0.23909535078319635 36-37 0.24093332959149835 38-39 0.25921430160955555 40-41 0.2590561959056156 42-43 0.28470884636987326 44-45 0.2616056503816474 46-47 0.2790368042410274 48-49 0.2637203141718443 50-51 0.2777521953965153 52-53 0.27745574720162786 54-55 0.2542537351484395 56-57 0.2703212273113374 58-59 0.2601431726202028 60-61 0.23682258128905953 62-63 0.22947066605585165 64-65 0.22767221367353463 66-67 0.23723760876190192 68-69 0.2421784120100255 70-71 0.22775126652550462 72-73 0.22004361345843185 74-75 0.22824534685031697 76-77 0.2292137442869492 78-79 0.23120982879919114 80-81 0.23875937616232396 82-83 0.2633645763379794 84-85 0.257751823848111 86-87 0.25767277099614105 88-89 0.2564276885776139 90-91 0.2479690334168263 92-93 0.22417412497386316 94-95 0.22320572753723092 96-97 0.21852184605800976 98-99 0.24184243738915306 100-101 0.25103233143066295 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2529953.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.181033424449716 #Duplication Level Percentage of deduplicated Percentage of total 1 92.30462198687339 57.3959678499697 2 3.7484703106313364 4.661675153518491 3 1.2516228725164078 2.334816110122456 4 0.5847752161775721 1.454477090517097 5 0.3673963471354004 1.1422542270623532 6 0.2691320178473541 1.0040944198413553 7 0.22032965839876872 0.9590228097303999 8 0.1365946580572016 0.6794877598604907 9 0.11743008201726879 0.6571731469456497 >10 0.8062530746948097 9.953223404172446 >50 0.09088722379766868 3.952873892891999 >100 0.09624605914401631 11.911413143954062 >500 0.004956486644563126 2.1397744189354726 >1k 0.0011570233503234184 1.118835936725129 >5k 1.269827138701525E-4 0.6349106357529303 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 8131 0.32138936968394277 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 7951 0.3142746130066448 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 4039 0.15964723455336918 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 3.162114078799092E-4 0.0 0.0 2 3.162114078799092E-4 0.0 0.0 3 3.9526425984988655E-4 0.0 0.0 4 4.3479068583487517E-4 0.0 0.0 5 5.138435378048525E-4 0.0 3.952642598498865E-5 6 6.324228157598184E-4 0.0 3.952642598498865E-5 7 7.905285196997731E-4 0.0 7.90528519699773E-5 8 9.881606496247163E-4 0.0 7.90528519699773E-5 9 0.0010672135015946937 0.0 7.90528519699773E-5 10-11 0.0016205834653845348 0.0 1.1857927795496596E-4 12-13 0.0024506384110692964 0.0 1.581057039399546E-4 14-15 0.0033004565697465522 0.0 1.581057039399546E-4 16-17 0.00438743328433374 0.0 1.7786891693244894E-4 18-19 0.005790621406800838 0.0 1.9763212992494328E-4 20-21 0.007253099168245418 0.0 1.9763212992494328E-4 22-23 0.009209657254502356 0.0 1.9763212992494328E-4 24-25 0.011087162488789317 0.0 2.3715855590993192E-4 26-27 0.013478511260881131 0.0 2.3715855590993192E-4 28-29 0.01612678180187537 0.0 2.3715855590993192E-4 30-31 0.024585436962662945 0.0 2.3715855590993192E-4 32-33 0.027569682124529586 0.0 2.3715855590993192E-4 34-35 0.03075155941632117 0.0 2.3715855590993192E-4 36-37 0.0348425445057675 0.0 2.5692176890242623E-4 38-39 0.0390125824471838 0.0 2.964481948874149E-4 40-41 0.04340001573151754 0.0 3.162114078799092E-4 42-43 0.047886265080813756 0.0 3.162114078799092E-4 44-45 0.05217488230018502 0.0 3.162114078799092E-4 46-47 0.05709592233531611 0.0 3.557378338648979E-4 48-49 0.06274820125116949 0.0 3.557378338648979E-4 50-51 0.06911195583475266 0.0 3.9526425984988655E-4 52-53 0.07531760471439589 0.0 3.9526425984988655E-4 54-55 0.08225449247476138 0.0 4.3479068583487517E-4 56-57 0.08996214554183418 0.0 4.3479068583487517E-4 58-59 0.09794648359080188 0.0 4.3479068583487517E-4 60-61 0.10685969265041682 0.0 4.3479068583487517E-4 62-63 0.11626698203484412 0.0 4.3479068583487517E-4 64-65 0.1263659838740087 0.0 4.5455389882736953E-4 66-67 0.13717646138090311 0.0 4.7431711181986384E-4 68-69 0.15022018195594938 0.0 4.7431711181986384E-4 70-71 0.1657935937940349 0.0 4.7431711181986384E-4 72-73 0.18219706057780521 0.0 4.7431711181986384E-4 74-75 0.19952939837222272 0.0 5.138435378048525E-4 76-77 0.21939142742967954 0.0 5.138435378048525E-4 78-79 0.24221793843601047 0.0 5.138435378048525E-4 80-81 0.268186800308148 0.0 5.138435378048525E-4 82-83 0.2988395436595067 0.0 5.138435378048525E-4 84-85 0.3335042192483417 0.0 5.138435378048525E-4 86-87 0.3736037784101128 0.0 5.138435378048525E-4 88-89 0.42044259320232436 0.0 5.138435378048525E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 450 0.0 57.945393 9 CGGGAGA 665 0.0 52.781914 4 AGAGCGG 515 0.0 52.474094 8 CGACGGG 105 0.0 49.717655 1 GTTCGGA 125 0.0 49.347275 2 GAGAGCG 680 0.0 48.80717 7 TTGTGTT 34400 0.0 46.809353 1 TCGGGAG 815 0.0 43.069157 3 TCGGGAA 200 0.0 42.690845 3 CGACGCC 45 9.677996E-4 42.177155 2 CGGACCG 60 8.3371604E-5 39.548134 1 TCGGAGA 250 0.0 37.945915 4 CGGAAAG 275 0.0 37.94591 5 TTCGGAG 255 0.0 37.20335 3 GATCCGG 220 0.0 36.671906 1 TGTGTTT 57850 0.0 36.00555 1 ATCGGGA 1160 0.0 34.768887 2 GCGTCGG 185 0.0 34.59639 10-11 AAGCGGC 180 0.0 34.24046 9 GATCGGG 1430 0.0 34.18286 1 >>END_MODULE