FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n02_50330665-2.34200000035755.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n02_50330665-2.34200000035755.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2529953
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG81310.32138936968394277No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC79510.3142746130066448No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA40390.15964723455336918No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC4500.057.9453939
CGGGAGA6650.052.7819144
AGAGCGG5150.052.4740948
CGACGGG1050.049.7176551
GTTCGGA1250.049.3472752
GAGAGCG6800.048.807177
TTGTGTT344000.046.8093531
TCGGGAG8150.043.0691573
TCGGGAA2000.042.6908453
CGACGCC459.677996E-442.1771552
CGGACCG608.3371604E-539.5481341
TCGGAGA2500.037.9459154
CGGAAAG2750.037.945915
TTCGGAG2550.037.203353
GATCCGG2200.036.6719061
TGTGTTT578500.036.005551
ATCGGGA11600.034.7688872
GCGTCGG1850.034.5963910-11
AAGCGGC1800.034.240469
GATCGGG14300.034.182861
Produced by FastQC (version 0.11.2)