##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n02_50330665-1.342000000356cf.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2871118 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.36052715353392 33.0 33.0 33.0 22.0 33.0 2 29.482008054005444 33.0 33.0 33.0 15.0 33.0 3 29.254502601425646 33.0 33.0 33.0 15.0 33.0 4 32.36146999182897 37.0 37.0 37.0 15.0 37.0 5 32.49662535639427 37.0 37.0 37.0 15.0 37.0 6 33.476972036677004 37.0 37.0 37.0 15.0 37.0 7 32.59310380137633 37.0 37.0 37.0 15.0 37.0 8 33.01613831267123 37.0 37.0 37.0 15.0 37.0 9 32.08294469262496 37.0 37.0 37.0 6.0 37.0 10-11 32.17318549777474 37.0 33.0 37.0 15.0 37.0 12-13 32.15865021918291 37.0 33.0 37.0 10.5 37.0 14-15 33.587382859220696 40.0 33.0 40.0 10.5 40.0 16-17 33.38546900545363 37.0 33.0 40.0 10.5 40.0 18-19 33.537135185666344 38.5 33.0 40.0 10.5 40.0 20-21 33.43285995211622 37.0 33.0 40.0 10.5 40.0 22-23 34.84036584354945 37.0 33.0 40.0 18.5 40.0 24-25 33.85338760023099 37.0 33.0 40.0 15.0 40.0 26-27 33.60135912212594 37.0 33.0 40.0 10.5 40.0 28-29 33.192117321545126 37.0 33.0 40.0 6.0 40.0 30-31 33.14221951170241 37.0 33.0 40.0 10.5 40.0 32-33 32.8823623759107 37.0 33.0 40.0 6.0 40.0 34-35 33.28402176434406 37.0 33.0 40.0 10.5 40.0 36-37 32.94934778020269 37.0 33.0 40.0 10.5 40.0 38-39 32.82023797001726 37.0 33.0 40.0 6.0 40.0 40-41 32.61227507890655 37.0 33.0 40.0 6.0 40.0 42-43 32.52074557715844 37.0 33.0 40.0 6.0 40.0 44-45 32.31915041457718 37.0 33.0 40.0 6.0 40.0 46-47 32.24919595781155 37.0 33.0 40.0 6.0 40.0 48-49 32.140215066047446 37.0 33.0 40.0 6.0 40.0 50-51 31.30005402076822 37.0 30.0 38.5 10.5 38.5 52-53 31.726405880914683 37.0 33.0 38.5 10.5 40.0 54-55 32.28858705911773 37.0 33.0 40.0 6.0 40.0 56-57 32.73838170357331 37.0 33.0 40.0 10.5 40.0 58-59 31.941220110075587 37.0 33.0 40.0 6.0 40.0 60-61 31.763044744242485 37.0 33.0 40.0 6.0 40.0 62-63 31.286945015844005 37.0 33.0 37.0 6.0 40.0 64-65 31.092723984176196 37.0 33.0 37.0 6.0 40.0 66-67 31.006083692833244 37.0 33.0 37.0 6.0 40.0 68-69 31.328632609318042 37.0 33.0 37.0 10.5 38.5 70-71 30.84940291551932 37.0 30.0 37.0 6.0 37.0 72-73 31.44725190674852 37.0 27.0 37.0 15.0 37.0 74-75 31.817269265839997 37.0 27.0 37.0 18.5 37.0 76-77 31.513417421366867 35.0 27.0 37.0 22.0 37.0 78-79 30.967932004187915 33.0 27.0 37.0 15.0 37.0 80-81 30.356969828477965 33.0 27.0 37.0 15.0 37.0 82-83 29.794071508032758 33.0 27.0 37.0 6.0 37.0 84-85 29.512838030342188 33.0 27.0 37.0 6.0 37.0 86-87 29.322129393497583 33.0 27.0 37.0 6.0 37.0 88-89 29.21155313017438 33.0 27.0 37.0 6.0 37.0 90-91 29.071845357801386 33.0 27.0 37.0 6.0 37.0 92-93 28.875816842080333 33.0 27.0 37.0 6.0 37.0 94-95 28.69519434589592 33.0 27.0 37.0 6.0 37.0 96-97 28.549024630823254 33.0 24.5 37.0 6.0 37.0 98-99 28.429586488608273 33.0 22.0 37.0 6.0 37.0 100-101 27.209368092847456 33.0 18.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -0.026833936319437868 2306 2 0.10800617885249508 2306 3 -0.07723336560141902 2306 4 0.24371174333040102 2306 5 0.02184538046616069 2306 6 -0.4796171256709698 2306 7 0.46716172988114124 2306 8 -0.4087992426149185 2306 9 0.15167172315855026 2306 10-11 0.030603827207073664 2306 12-13 0.05534209506281229 2306 14-15 0.45956764330779976 2306 16-17 0.4076371676671684 2306 18-19 0.3650529256974835 2306 20-21 0.41768479037673956 2306 22-23 -0.07547616004703883 2306 24-25 0.43729889142865375 2306 26-27 -0.15200568009000648 2306 28-29 0.22355858464116807 2306 30-31 0.27266135632876143 2306 32-33 0.2106326315720679 2306 34-35 -0.2268147671443117 2306 36-37 0.48047229133756275 2306 38-39 0.012736859458385652 2306 40-41 0.057247894411624145 2306 42-43 0.17572583505700123 2306 44-45 0.2272734987499021 2306 46-47 0.1293690036852979 2306 48-49 0.2082626160022798 2306 50-51 0.07465366112125338 2306 52-53 -0.002128431242759632 2306 54-55 0.29344403090708227 2306 56-57 -0.024704339909810358 2306 58-59 0.40322019711813795 2306 60-61 0.34083045765332187 2306 62-63 0.44254366235868403 2306 64-65 0.26462524867252313 2306 66-67 0.15543727788756811 2306 68-69 -0.2320879351116325 2306 70-71 0.19723075943884538 2306 72-73 -0.4871102727731511 2306 74-75 -0.6062131589288136 2306 76-77 -0.2990497434978039 2306 78-79 0.013017578036279787 2306 80-81 0.22724834361268265 2306 82-83 0.3524134034563495 2306 84-85 0.41681844099497667 2306 86-87 0.36449331766043613 2306 88-89 0.43138068209794156 2306 90-91 0.12026632512836954 2306 92-93 0.24099900700488064 2306 94-95 0.07446143150061246 2306 96-97 0.3341169384534801 2306 98-99 0.21115166439301802 2306 100-101 -0.07012108902664238 2307 1 -0.12725331580897148 2307 2 -0.021471430806681013 2307 3 -0.2118425662738943 2307 4 0.09411064291774807 2307 5 -0.06759065804828168 2307 6 -0.47276522287408795 2307 7 0.4607408220406981 2307 8 -0.4387298554836363 2307 9 0.007083002388263537 2307 10-11 0.07359144756470926 2307 12-13 0.01649294329756401 2307 14-15 0.5397724423296566 2307 16-17 0.3252673067471008 2307 18-19 0.44009464967731304 2307 20-21 0.37566675583600784 2307 22-23 0.1934203705963924 2307 24-25 0.43899658361878124 2307 26-27 -0.08435231007624822 2307 28-29 0.16705728554289578 2307 30-31 0.19591277005335428 2307 32-33 0.34246359596149034 2307 34-35 -0.04325462653601875 2307 36-37 0.3449870399238364 2307 38-39 0.026919955897326986 2307 40-41 0.16740286919383607 2307 42-43 0.14900200806631858 2307 44-45 0.21380872723835864 2307 46-47 0.20178746616429066 2307 48-49 0.13856125576997158 2307 50-51 0.05423794967390805 2307 52-53 0.05001278838126666 2307 54-55 0.21247872447272442 2307 56-57 0.12879986305519964 2307 58-59 0.27249789847837036 2307 60-61 0.19981471563895425 2307 62-63 0.3309694609220344 2307 64-65 0.33491135596275257 2307 66-67 0.12544736500356635 2307 68-69 -0.07957929992899437 2307 70-71 0.09961406984691479 2307 72-73 -0.45069731235744115 2307 74-75 -0.30096816427804285 2307 76-77 0.08901139053857321 2307 78-79 0.2714173946341738 2307 80-81 0.4002188464401293 2307 82-83 0.3301566405035743 2307 84-85 0.3813585602063476 2307 86-87 0.43109885029072714 2307 88-89 0.517850802837664 2307 90-91 0.5385933922229746 2307 92-93 0.33360117726317284 2307 94-95 0.35362053283027706 2307 96-97 0.3913542913497068 2307 98-99 0.20006195019464101 2307 100-101 0.20166952689877604 2201 1 -0.20133216885556493 2201 2 -0.15739974621886788 2201 3 -0.2448031397505943 2201 4 -0.13642699149085047 2201 5 -0.1994186150375299 2201 6 -0.44257167448699164 2201 7 0.2681816822557508 2201 8 -0.3937549450893698 2201 9 0.010996980882886476 2201 10-11 -0.11703220834927208 2201 12-13 0.019826276630894313 2201 14-15 0.3126039835482928 2201 16-17 0.2813174859585672 2201 18-19 0.2016215313977412 2201 20-21 0.11456281318368156 2201 22-23 -0.011561708256657255 2201 24-25 0.4227098452675264 2201 26-27 -0.19183618104398903 2201 28-29 0.06298560095508066 2201 30-31 0.15389843313579377 2201 32-33 0.20694388269983932 2201 34-35 -0.18842308531738894 2201 36-37 0.32319492522849913 2201 38-39 7.550813453534033E-4 2201 40-41 4.781380110401301E-4 2201 42-43 0.08062207975090985 2201 44-45 0.10850406774014942 2201 46-47 0.10844696437217749 2201 48-49 0.10095552100294469 2201 50-51 0.04140640845526988 2201 52-53 -0.011707641726257378 2201 54-55 0.1295683302074906 2201 56-57 0.2038034472845851 2201 58-59 0.47517890206259494 2201 60-61 0.2894849665350101 2201 62-63 0.29638164730196337 2201 64-65 0.30757802262942135 2201 66-67 0.21710327898206216 2201 68-69 -0.029443099212148383 2201 70-71 0.17881837092218333 2201 72-73 -0.1996937281280502 2201 74-75 -0.08883196356119427 2201 76-77 0.12430888157799913 2201 78-79 0.28120234087073115 2201 80-81 0.48244823712113316 2201 82-83 0.34979104910572545 2201 84-85 0.31183167848591964 2201 86-87 0.42780135925130125 2201 88-89 0.3403525949523569 2201 90-91 0.21528873272476545 2201 92-93 0.3150061951843206 2201 94-95 0.33385709197006364 2201 96-97 0.2850030368694192 2201 98-99 0.2491658928469711 2201 100-101 0.05371970611024324 1101 1 -0.13482690156511268 1101 2 -0.15116536815173376 1101 3 -0.02515516487655134 1101 4 0.17343451039577218 1101 5 0.07462705662379676 1101 6 -0.21792817081188076 1101 7 0.49281384435307274 1101 8 -0.24158674076648623 1101 9 0.26356170421382075 1101 10-11 0.07494100807856796 1101 12-13 0.08888645242535631 1101 14-15 0.3798625933325894 1101 16-17 0.4423090015859472 1101 18-19 0.3142704441330224 1101 20-21 0.3464614369268304 1101 22-23 0.017894114116792537 1101 24-25 0.23650120385767792 1101 26-27 0.020605093574864952 1101 28-29 0.15907711876354114 1101 30-31 0.22421883270830278 1101 32-33 -0.03505049013271844 1101 34-35 -0.26426116248374143 1101 36-37 0.21400214021682729 1101 38-39 0.08849309089845292 1101 40-41 0.05534607406199399 1101 42-43 0.21474147166785684 1101 44-45 0.27639606102244585 1101 46-47 0.1565077726776991 1101 48-49 0.14561647325926685 1101 50-51 0.14869817054386658 1101 52-53 -0.002373959421301919 1101 54-55 0.21531938257505345 1101 56-57 0.17308609248949125 1101 58-59 0.3359741211513452 1101 60-61 0.2308027706310689 1101 62-63 0.2676992332907524 1101 64-65 0.25482616326498686 1101 66-67 0.1960166685870668 1101 68-69 -0.007250248768301049 1101 70-71 0.23464990487057946 1101 72-73 -0.19127653255126376 1101 74-75 -0.20535446132559443 1101 76-77 0.1496703943699913 1101 78-79 0.41635992336980365 1101 80-81 0.40670656336597943 1101 82-83 0.35231891230884926 1101 84-85 0.4427121775153431 1101 86-87 0.43808555301779606 1101 88-89 0.3482393536602899 1101 90-91 0.450442389293066 1101 92-93 0.2508957456652432 1101 94-95 0.3718666450682768 1101 96-97 0.3375485667610185 1101 98-99 0.24160803989488855 1101 100-101 0.09503576534817881 2304 1 -0.13130119506996252 2304 2 -0.28588152708563186 2304 3 -0.11372963366915556 2304 4 0.04475857422141871 2304 5 -0.09018238326295602 2304 6 -0.37779566783896 2304 7 0.3529375433521764 2304 8 -0.35677929602487524 2304 9 -0.06540099448917402 2304 10-11 0.05438301665135725 2304 12-13 -0.09320260705372618 2304 14-15 0.19615287428386097 2304 16-17 0.11964565699430096 2304 18-19 0.18774023906060933 2304 20-21 0.22964125492526222 2304 22-23 -0.01790931974969112 2304 24-25 0.14523962291099934 2304 26-27 -0.18466612740646582 2304 28-29 0.03967971593581865 2304 30-31 0.2461381148360715 2304 32-33 0.08444381974499038 2304 34-35 -0.19012790262241452 2304 36-37 0.26828394338467376 2304 38-39 0.033607439633982494 2304 40-41 0.17829436021647638 2304 42-43 0.24547715768900957 2304 44-45 0.17818551620531053 2304 46-47 0.11279865533941091 2304 48-49 0.16234631952224277 2304 50-51 0.09934073395702114 2304 52-53 -0.03563904612302338 2304 54-55 0.20587086602880333 2304 56-57 0.20889926456442964 2304 58-59 0.4052499151320248 2304 60-61 0.1639094333074418 2304 62-63 0.30430539638911824 2304 64-65 0.2687958212242698 2304 66-67 0.16776430489427696 2304 68-69 0.18414331261329409 2304 70-71 0.23084123612326124 2304 72-73 -0.07026535815499457 2304 74-75 0.04537115396291469 2304 76-77 0.11132885085603306 2304 78-79 0.28560943210489853 2304 80-81 0.4104072409236075 2304 82-83 0.27823782707656264 2304 84-85 0.37658728776294836 2304 86-87 0.4094230761559352 2304 88-89 0.5314016745524697 2304 90-91 0.17155878384410528 2304 92-93 0.3522074744268444 2304 94-95 0.27930671550006636 2304 96-97 0.38324760386597134 2304 98-99 0.34365029263544145 2304 100-101 0.23096278737236275 2202 1 -0.355287739803515 2202 2 -0.5815477770916182 2202 3 -0.3985845567306079 2202 4 -0.52371655544626 2202 5 -0.5156683676597318 2202 6 -0.5121537709313486 2202 7 -0.030259177959301553 2202 8 -0.616731559641778 2202 9 -0.4476470589614294 2202 10-11 -0.386483194562075 2202 12-13 -0.43494877449384717 2202 14-15 -0.38133014799072384 2202 16-17 -0.42782642195091825 2202 18-19 -0.38138592291982576 2202 20-21 -0.354156182132634 2202 22-23 -0.4541196771200333 2202 24-25 -0.3414751273559986 2202 26-27 -0.5757771567020171 2202 28-29 -0.3653408057999812 2202 30-31 -0.2816236989309715 2202 32-33 -0.37565927384083153 2202 34-35 -0.8130883007009899 2202 36-37 -0.25170604119409035 2202 38-39 -0.5558041408988572 2202 40-41 -0.48223823510064534 2202 42-43 -0.4108217819813973 2202 44-45 -0.46155340636763853 2202 46-47 -0.32704416301090333 2202 48-49 -0.2731495485926736 2202 50-51 -0.18277551317544294 2202 52-53 -0.4024897678559505 2202 54-55 -0.25658552228937737 2202 56-57 -0.1976023182672364 2202 58-59 -0.21432197200561376 2202 60-61 -0.28166261905770185 2202 62-63 -0.03733660588096299 2202 64-65 -0.23935704894521947 2202 66-67 -0.2686052934831409 2202 68-69 -0.25797722085605557 2202 70-71 -0.06273153342506887 2202 72-73 -0.4146756695489877 2202 74-75 -0.1732275371478451 2202 76-77 0.03804700744382217 2202 78-79 0.21444142326403082 2202 80-81 0.12482331133447389 2202 82-83 -0.03259029201176489 2202 84-85 -0.10034491627626707 2202 86-87 -0.10757275843456426 2202 88-89 -0.05515090132048073 2202 90-91 -0.06874113402106019 2202 92-93 -0.13470932035100347 2202 94-95 -0.10496637923516161 2202 96-97 -0.08891649937967472 2202 98-99 -0.07521327134591971 2202 100-101 -0.21075395640047745 1102 1 -0.39587293216800035 1102 2 -0.49485109059676446 1102 3 -0.39509699986057356 1102 4 -0.38057629157374606 1102 5 -0.3700227970276515 1102 6 -0.4764250129573284 1102 7 0.004530905283552045 1102 8 -0.6297265981546474 1102 9 -0.4008779096638513 1102 10-11 -0.23096446991628738 1102 12-13 -0.25158885185263813 1102 14-15 -0.20651058336704864 1102 16-17 -0.3092654475055241 1102 18-19 -0.24445764854925045 1102 20-21 -0.20056053334242563 1102 22-23 -0.3036806066023061 1102 24-25 -0.27089411489732385 1102 26-27 -0.453659584777661 1102 28-29 -0.2519770973742155 1102 30-31 -0.08826891651921187 1102 32-33 -0.22571229980398755 1102 34-35 -0.3986270478172358 1102 36-37 -0.07460036507072942 1102 38-39 -0.19758239661805987 1102 40-41 -0.07906980543518927 1102 42-43 -0.1555531855637895 1102 44-45 -0.0747906212958398 1102 46-47 -0.17359943213466522 1102 48-49 -0.12499429978558396 1102 50-51 -0.27736538004016964 1102 52-53 -0.272122571734549 1102 54-55 -1.5392120226209727E-4 1102 56-57 0.06293015621480436 1102 58-59 0.1467128821917285 1102 60-61 -0.03930725323545303 1102 62-63 0.11317358687209023 1102 64-65 0.008215011409731687 1102 66-67 -0.0639540103131182 1102 68-69 -0.18704365027282321 1102 70-71 -0.1398191549087855 1102 72-73 -0.165952469795009 1102 74-75 0.22384616796951562 1102 76-77 0.21045764461023353 1102 78-79 0.12733125637865328 1102 80-81 -0.10306960813822741 1102 82-83 0.1007559890377756 1102 84-85 0.046785270304070536 1102 86-87 0.25618028351548006 1102 88-89 0.26206578655101964 1102 90-91 0.2241981696388251 1102 92-93 0.08505394599281502 1102 94-95 0.0012651220448987033 1102 96-97 0.10012591639494772 1102 98-99 0.19099933709293637 1102 100-101 0.15231520188972425 2305 1 -0.3718407010852829 2305 2 -0.6555889859716899 2305 3 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1213 54-55 -0.8459492631570527 1213 56-57 -0.869425672619645 1213 58-59 -0.8837398969711572 1213 60-61 -0.24639988564224424 1213 62-63 -0.8152405015255049 1213 64-65 -0.7244514670988735 1213 66-67 -0.4203531651488497 1213 68-69 0.10216445973524202 1213 70-71 -1.1465651879396965 1213 72-73 -0.07923166181624453 1213 74-75 -0.41098351660850696 1213 76-77 -0.670918143369093 1213 78-79 -0.8282628571882249 1213 80-81 -1.1549874712095267 1213 82-83 -1.3923165205302226 1213 84-85 -1.1636003529811916 1213 86-87 -1.1781512049334815 1213 88-89 -1.2453723029719228 1213 90-91 -1.1538344849583808 1213 92-93 -1.1403648046232853 1213 94-95 -1.2509646229019964 1213 96-97 -1.2279202834238738 1213 98-99 -1.195820532685854 1213 100-101 -0.9876062628074322 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 304.0 3 264.0 4 409.0 5 264.0 6 688.0 7 4985.0 8 27818.0 9 98971.0 10 128657.0 11 76796.0 12 39204.0 13 23848.0 14 16765.0 15 13026.0 16 11461.0 17 10295.0 18 9885.0 19 9949.0 20 10012.0 21 10443.0 22 10959.0 23 11337.0 24 12722.0 25 14274.0 26 17053.0 27 20973.0 28 26834.0 29 34011.0 30 39733.0 31 51525.0 32 71798.0 33 103213.0 34 159745.0 35 279730.0 36 531397.0 37 814676.0 38 177019.0 39 75.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.08304431626121 18.95984558463327 21.99169863881682 11.965411460288705 2 34.63760298977359 25.101803577010674 30.072909223864603 10.187684209351136 3 39.458814863076626 17.856368051374954 29.703726232770787 12.981090852777635 4 29.769829195936754 14.39153450225337 35.78180159329702 20.05683470851286 5 38.16510984047618 12.460073703646607 32.08738409254042 17.287432363336798 6 36.13857829927076 12.652528329678791 37.10699719353453 14.101896177515915 7 42.098604286639656 16.47844024830966 25.81613008715103 15.60682537789966 8 29.329231138198796 35.57005453326027 20.070428917826423 15.03028541071451 9 37.78484017577214 27.620913064181373 18.566228630668654 16.028018129377834 10-11 32.33050671720677 30.03448957279422 22.903589167011994 14.73141454298702 12-13 36.10401543820118 21.585751594434885 22.557976915204232 19.7522560521597 14-15 31.02579901293916 21.79016547169499 24.84217571704837 22.341859798317483 16-17 33.08392062760605 23.427533963607157 26.192377595417742 17.296167813369046 18-19 32.68104875133173 23.536150320164424 26.697465750571688 17.085335177932155 20-21 29.62664301125491 27.78903230079088 25.629790482298525 16.954534205655687 22-23 35.136074076591946 23.374375628076102 24.19715641132241 17.29239388400954 24-25 29.406535123727718 28.74423215566874 24.752241986583588 17.096990734019954 26-27 23.65189448799703 32.74683733956197 25.480253257305385 18.121014915135614 28-29 26.143360431619804 30.675166134360214 23.9296769651314 19.25179646888858 30-31 29.93362066528039 25.689441336355358 25.26422054024317 19.112717458121082 32-33 25.31466033548755 27.737544482277187 27.195577811752177 19.752217370483095 34-35 32.50442288641888 24.004119590310236 24.356571488420197 19.134886034850688 36-37 26.789745451752438 25.30157560861505 27.566011123175375 20.342667816457134 38-39 19.959661825585233 29.94852797264882 29.37282205023171 20.718988151534234 40-41 22.848406736764478 31.731963176302326 25.37621237177911 20.04341771515409 42-43 28.694664170020616 25.79107319804362 27.059689330330933 18.45457330160483 44-45 23.650272315739244 29.33837393465295 27.41032996185502 19.601023787752784 46-47 26.472587250168083 30.57059034114226 24.514359059435765 18.442463349253888 48-49 21.245341362762158 28.51354005795087 29.30681573141805 20.93430284786892 50-51 18.57728990053952 25.575287768307987 31.053446365470773 24.793975965681724 52-53 27.454768471643572 25.656054928501653 24.89713479227707 21.99204180757771 54-55 31.052775842774118 25.255568882347703 25.18376133286544 18.50789394201274 56-57 32.52572842641455 24.886614722642168 23.337775500817713 19.249881350125563 58-59 28.9051130829156 24.336558753234165 23.534135087715928 23.22419307613431 60-61 22.14082307150111 24.029584379648632 24.750987722807118 29.078604826043136 62-63 26.571447421885804 23.72434766945514 25.433909598792454 24.270295309866608 64-65 23.525027743054114 27.88663030892445 28.001494756309693 20.586847191711747 66-67 20.814920608543318 27.35407661043411 26.608793281976695 25.222209499045878 68-69 19.202708111819238 23.480636605978425 36.25124747602673 21.06540780617561 70-71 18.640472863826492 31.51691860064283 30.637847781901772 19.204760753628904 72-73 20.467169279969617 37.58918744958363 24.20074676436432 17.742896506082424 74-75 21.53675076697731 36.951908162048866 23.765553577228946 17.74578749374488 76-77 20.708319646393473 35.90054362605174 23.85034068698722 19.540796040567564 78-79 20.626582361334748 36.33767514519979 24.246719641135066 18.789022852330397 80-81 19.79778253077486 36.86676559401884 24.187126654998103 19.1483252202082 82-83 19.69305519883632 35.83691163224032 24.41963987178012 20.05039329714323 84-85 20.46742795726409 34.27426155475499 24.62465356598754 20.633656921993378 86-87 19.43468493005274 34.257711238491105 25.437711601635826 20.869892229820326 88-89 19.143393971941126 34.37782721296467 25.49709190975824 20.981686905335962 90-91 20.199564545817655 32.922720848451334 24.869053005862853 22.008661599868162 92-93 20.834905582990174 32.83820704547033 24.046728016110073 22.28015935542942 94-95 21.182366257920016 34.06096625104348 23.454049119061633 21.30261837197487 96-97 20.630068849969895 34.47332827211881 22.834559368918907 22.06204350899238 98-99 20.791358479637616 32.573877168117676 24.47726109093626 22.157503261308445 100-101 21.94620363932064 33.534520789147365 23.73969498391891 20.779580587613083 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 660.0 1 485.5 2 313.5 3 469.0 4 515.5 5 385.5 6 436.0 7 594.0 8 743.0 9 866.5 10 928.0 11 1011.0 12 1100.5 13 1090.5 14 1001.0 15 918.0 16 846.0 17 763.5 18 729.0 19 680.5 20 672.0 21 726.5 22 798.0 23 894.0 24 992.5 25 1189.5 26 1474.0 27 1821.0 28 2282.0 29 2860.5 30 3612.0 31 4837.5 32 7269.5 33 11361.0 34 17670.0 35 28253.5 36 42655.0 37 61555.0 38 89696.5 39 114190.5 40 126302.5 41 128665.5 42 151272.5 43 190752.5 44 191529.5 45 174535.5 46 179802.5 47 183037.0 48 166454.0 49 143585.5 50 122303.5 51 110943.5 52 105836.0 53 105005.5 54 95169.5 55 66148.0 56 44270.5 57 36790.0 58 33129.5 59 27629.0 60 20954.0 61 15641.5 62 11155.5 63 8003.5 64 5935.0 65 4186.0 66 2968.0 67 2270.0 68 1765.5 69 1352.5 70 1002.0 71 773.0 72 617.0 73 475.5 74 368.0 75 283.5 76 222.5 77 171.5 78 133.5 79 101.0 80 92.0 81 84.0 82 66.0 83 48.5 84 44.5 85 47.0 86 40.0 87 31.0 88 24.5 89 15.0 90 14.0 91 14.0 92 10.0 93 6.5 94 6.5 95 7.0 96 4.5 97 2.0 98 1.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17700421926232218 2 0.16766987633388805 3 0.14071173668236556 4 0.13430308332851523 5 0.13792536565895236 6 0.12030156893586401 7 0.09626911885892533 8 0.08393942708032201 9 0.08373044925356604 10-11 0.06426068172746643 12-13 0.08324283432446873 14-15 0.08709150930055817 16-17 0.08052612257664088 18-19 0.07667744760055142 20-21 0.07481406197864386 22-23 0.0952764741818344 24-25 0.43122574551098214 26-27 0.27114873021589503 28-29 0.1187342352351941 30-31 0.22189962237706704 32-33 0.2506514883749118 34-35 0.23529161810834665 36-37 0.23781676684831482 38-39 0.2552838302013362 40-41 0.2546046522643792 42-43 0.2813189844513531 44-45 0.25908026072073664 46-47 0.27665181298713604 48-49 0.2607172536969919 50-51 0.27586814613680105 52-53 0.2753282867510148 54-55 0.25122617739849074 56-57 0.2664815587516779 58-59 0.25622423042173814 60-61 0.23339340284864643 62-63 0.2252084379673702 64-65 0.22419837847138294 66-67 0.23349789176202443 68-69 0.23804315949396715 70-71 0.22449443039262057 72-73 0.216814495259338 74-75 0.22512136387288856 76-77 0.22517360832957756 78-79 0.2274549496049971 80-81 0.23428155861235936 82-83 0.2592544089097 84-85 0.2541518669730746 86-87 0.2523755554456487 88-89 0.2511565181229054 90-91 0.2431805310683852 92-93 0.22036711831418979 94-95 0.2195486218260622 96-97 0.21569994684997273 98-99 0.2387049226120278 100-101 0.24518323524146346 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2871118.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.40634817523441 #Duplication Level Percentage of deduplicated Percentage of total 1 85.69378505407926 33.76879130294746 2 5.584898589161429 4.401609166557414 3 2.165248343415825 2.559735903194806 4 1.4673198972734265 2.3128687502562335 5 0.9098040366880545 1.7926027320481608 6 0.69726677963629 1.6486042493623256 7 0.4461355210517636 1.2306400173133791 8 0.35091852244612415 1.1062733981320623 9 0.2767419571014907 0.9814850924613415 >10 1.9619242244950712 15.379091354780245 >50 0.2335453741938736 6.4029296095379715 >100 0.1882146668016419 15.355251901487573 >500 0.016763687087268206 4.417128216006423 >1k 0.006633942856662464 4.562483514654909 >5k 2.6646790403169276E-4 0.7251313930576951 >10k+ 5.329358076903914E-4 3.35537339820186 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 22128 0.7707102250760853 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT 21301 0.7419061146215515 Illumina Single End PCR Primer 1 (97% over 34bp) GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 20700 0.72097350230816 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 11600 0.404023798394911 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 10082 0.35115240822564586 No Hit TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 10052 0.35010751909186594 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 7900 0.27515413856205145 No Hit TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 6876 0.23948858946236276 No Hit TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 5941 0.20692287812622123 No Hit GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC 4562 0.15889280761013652 No Hit GTGTTTGATCGGGGTTTATCGATTACAGAACAGGCTCCTCTAGAGGGATA 4238 0.14760800496531318 No Hit TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 4111 0.14318464096564476 No Hit GTGTTTGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAA 3631 0.12646641482516566 No Hit AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC 3325 0.11580854566061026 No Hit GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC 3174 0.11054927035391787 No Hit TTGTGTTTGATCGGGGTTTATCGATTACAGAACAGGCTCCTCTAGAGGGA 2956 0.10295640931511697 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.007418712849837589 0.0 0.0 2 0.00808047596789822 0.0 0.0 3 0.008811898361544179 0.0 3.482963779266474E-5 4 0.00940400220401948 0.0 3.482963779266474E-5 5 0.01118031373144538 0.0 3.482963779266474E-5 6 0.012294862140810653 0.0 3.482963779266474E-5 7 0.014001514392651225 0.0 3.482963779266474E-5 8 0.014976744250845837 0.0 3.482963779266474E-5 9 0.016648566864893747 0.0 3.482963779266474E-5 10-11 0.02551270968312692 0.0 3.482963779266474E-5 12-13 0.03200843713145889 0.0 3.482963779266474E-5 14-15 0.03862606831206519 0.0 3.482963779266474E-5 16-17 0.044163980721098894 0.0 3.482963779266474E-5 18-19 0.05036365624819321 0.0 3.482963779266474E-5 20-21 0.055448783365922266 0.0 3.482963779266474E-5 22-23 0.06082996240488897 0.0 3.482963779266474E-5 24-25 0.06621114144385568 0.0 3.482963779266474E-5 26-27 0.07221925396309034 0.0 3.482963779266474E-5 28-29 0.07899361851376363 0.0 3.482963779266474E-5 30-31 0.15891022242903288 0.0 3.482963779266474E-5 32-33 0.17117025493205085 0.0 3.482963779266474E-5 34-35 0.17959902727787574 0.0 3.482963779266474E-5 36-37 0.19003050379677883 0.0 3.482963779266474E-5 38-39 0.20213380292972982 0.0 3.482963779266474E-5 40-41 0.21603082840900306 0.0 3.482963779266474E-5 42-43 0.2325226619038298 0.0 3.482963779266474E-5 44-45 0.2502683623591925 0.0 3.482963779266474E-5 46-47 0.26874548520820113 0.0 3.482963779266474E-5 48-49 0.28971292715938535 0.0 3.482963779266474E-5 50-51 0.3119342360711054 0.0 3.482963779266474E-5 52-53 0.33774299767547 0.0 3.482963779266474E-5 54-55 0.3659898339253211 0.0 3.482963779266474E-5 56-57 0.39698821156079267 0.0 3.482963779266474E-5 58-59 0.4309471084086408 0.0 3.482963779266474E-5 60-61 0.4654632794611716 0.0 3.482963779266474E-5 62-63 0.5038455403086881 0.0 3.482963779266474E-5 64-65 0.5465815058802878 0.0 3.482963779266474E-5 66-67 0.5924695536721236 0.0 3.482963779266474E-5 68-69 0.6406563575582752 0.0 3.482963779266474E-5 70-71 0.6952866444360699 0.0 3.482963779266474E-5 72-73 0.7551936214394532 0.0 3.482963779266474E-5 74-75 0.8202379700172546 0.0 3.482963779266474E-5 76-77 0.8936588464841919 0.0 3.482963779266474E-5 78-79 0.9769539252653496 0.0 3.482963779266474E-5 80-81 1.067145272329455 0.0 3.482963779266474E-5 82-83 1.1719650672664796 0.0 3.482963779266474E-5 84-85 1.2937294809896356 0.0 3.482963779266474E-5 86-87 1.4287988163495893 0.0 3.482963779266474E-5 88-89 1.5810739927791195 0.0 3.482963779266474E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 4050 0.0 89.453384 9 ATTCGGA 1050 0.0 89.031944 2 GTTCGGA 1045 0.0 87.641525 2 CGGGAGA 5405 0.0 87.51552 4 AAGCGGC 1340 0.0 87.05401 9 ACCGGAA 610 0.0 86.33789 3 AGAGCGG 4565 0.0 83.10272 8 TCGGAGA 1545 0.0 81.99142 4 GGATCGG 2655 0.0 81.69801 1 ATCCGGA 1030 0.0 81.546425 2 ATCGGGA 6935 0.0 80.9482 2 TCGGGAG 5975 0.0 80.75908 3 CGGAAAG 3015 0.0 80.254326 5 TTGGGAG 3155 0.0 76.99791 3 TCCGGAA 840 0.0 76.25403 3 CGGAGAG 2360 0.0 74.38328 5 GGTTCGG 1435 0.0 73.75862 1 AAGGGGC 4090 0.0 73.62222 9 GGAAAGC 1950 0.0 73.21693 6 GTCCGGA 590 0.0 73.1911 2 >>END_MODULE