FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n02_50330665-1.342000000356cf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n02_50330665-1.342000000356cf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2871118
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA221280.7707102250760853No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT213010.7419061146215515Illumina Single End PCR Primer 1 (97% over 34bp)
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC207000.72097350230816No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC116000.404023798394911No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT100820.35115240822564586No Hit
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT100520.35010751909186594No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC79000.27515413856205145No Hit
TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT68760.23948858946236276No Hit
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT59410.20692287812622123No Hit
GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC45620.15889280761013652No Hit
GTGTTTGATCGGGGTTTATCGATTACAGAACAGGCTCCTCTAGAGGGATA42380.14760800496531318No Hit
TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT41110.14318464096564476No Hit
GTGTTTGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAA36310.12646641482516566No Hit
AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC33250.11580854566061026No Hit
GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC31740.11054927035391787No Hit
TTGTGTTTGATCGGGGTTTATCGATTACAGAACAGGCTCCTCTAGAGGGA29560.10295640931511697No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC40500.089.4533849
ATTCGGA10500.089.0319442
GTTCGGA10450.087.6415252
CGGGAGA54050.087.515524
AAGCGGC13400.087.054019
ACCGGAA6100.086.337893
AGAGCGG45650.083.102728
TCGGAGA15450.081.991424
GGATCGG26550.081.698011
ATCCGGA10300.081.5464252
ATCGGGA69350.080.94822
TCGGGAG59750.080.759083
CGGAAAG30150.080.2543265
TTGGGAG31550.076.997913
TCCGGAA8400.076.254033
CGGAGAG23600.074.383285
GGTTCGG14350.073.758621
AAGGGGC40900.073.622229
GGAAAGC19500.073.216936
GTCCGGA5900.073.19112
Produced by FastQC (version 0.11.2)