##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n02_50220371-2.34200000035902.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3444146 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.26036294628625 33.0 33.0 33.0 33.0 33.0 2 32.27593690859795 33.0 33.0 33.0 33.0 33.0 3 32.27322767385587 33.0 33.0 33.0 33.0 33.0 4 36.1017535261281 37.0 37.0 37.0 33.0 37.0 5 36.10649548538302 37.0 37.0 37.0 33.0 37.0 6 36.0337131468875 37.0 37.0 37.0 33.0 37.0 7 35.999094695753314 37.0 37.0 37.0 33.0 37.0 8 35.93610288297883 37.0 37.0 37.0 33.0 37.0 9 35.93190648712337 37.0 37.0 37.0 33.0 37.0 10-11 35.83378825984729 37.0 37.0 37.0 33.0 37.0 12-13 35.84559481508624 37.0 37.0 37.0 33.0 37.0 14-15 37.663107342139384 40.0 37.0 40.0 33.0 40.0 16-17 37.47628599368319 40.0 37.0 40.0 33.0 40.0 18-19 37.18461078595391 40.0 37.0 40.0 33.0 40.0 20-21 37.042438531932156 40.0 37.0 40.0 33.0 40.0 22-23 36.96133323035667 40.0 37.0 40.0 33.0 40.0 24-25 36.76676351699376 40.0 37.0 40.0 33.0 40.0 26-27 36.69399671210222 40.0 37.0 40.0 33.0 40.0 28-29 36.683953148327625 40.0 37.0 40.0 33.0 40.0 30-31 36.6175808168411 40.0 37.0 40.0 33.0 40.0 32-33 36.51880117741815 37.0 37.0 40.0 33.0 40.0 34-35 36.43838370963368 37.0 37.0 40.0 33.0 40.0 36-37 36.3350747906738 37.0 37.0 40.0 33.0 40.0 38-39 36.26091504250981 37.0 37.0 40.0 33.0 40.0 40-41 36.140703239641994 37.0 37.0 40.0 33.0 40.0 42-43 36.042875940799256 37.0 37.0 40.0 33.0 40.0 44-45 35.87635468995798 37.0 37.0 40.0 27.0 40.0 46-47 35.76095264254187 37.0 37.0 40.0 27.0 40.0 48-49 35.608465785132225 37.0 37.0 40.0 27.0 40.0 50-51 34.29342164937259 37.0 35.0 38.5 24.5 38.5 52-53 34.73508672396582 37.0 35.0 38.5 27.0 40.0 54-55 35.492656234666015 37.0 37.0 40.0 27.0 40.0 56-57 35.45635870256371 37.0 37.0 40.0 27.0 40.0 58-59 35.28811597998459 37.0 37.0 40.0 27.0 40.0 60-61 35.004674017884255 37.0 35.0 40.0 27.0 40.0 62-63 34.7366547469242 37.0 33.0 40.0 27.0 40.0 64-65 34.480689988171235 37.0 33.0 38.5 27.0 40.0 66-67 34.20987800749445 37.0 33.0 37.0 27.0 40.0 68-69 33.96108991895233 37.0 33.0 37.0 27.0 40.0 70-71 33.60329265948656 37.0 33.0 37.0 22.0 40.0 72-73 33.30553829599558 37.0 33.0 37.0 22.0 38.5 74-75 33.016216211507874 37.0 33.0 37.0 22.0 37.0 76-77 32.75363907337261 37.0 33.0 37.0 22.0 37.0 78-79 32.52769859349749 37.0 33.0 37.0 22.0 37.0 80-81 32.27995967650617 37.0 33.0 37.0 22.0 37.0 82-83 32.04153613116285 37.0 33.0 37.0 18.5 37.0 84-85 31.827641453062675 37.0 33.0 37.0 15.0 37.0 86-87 31.638267512468985 37.0 33.0 37.0 15.0 37.0 88-89 31.431875274741547 33.0 33.0 37.0 15.0 37.0 90-91 31.21895645538836 33.0 33.0 37.0 15.0 37.0 92-93 31.03547062174484 33.0 33.0 37.0 15.0 37.0 94-95 30.86168704811004 33.0 33.0 37.0 15.0 37.0 96-97 30.67418425351306 33.0 30.0 37.0 15.0 37.0 98-99 30.473015952285415 33.0 27.0 37.0 15.0 37.0 100-101 29.23061522362873 33.0 27.0 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.2502136023418373 2306 2 0.1988290291259318 2306 3 0.10926521240333642 2306 4 0.25635957015889943 2306 5 0.22250680404546586 2306 6 0.2682723494571633 2306 7 0.290626152030832 2306 8 0.25218110209937805 2306 9 0.22173518633434952 2306 10-11 0.25260920752423033 2306 12-13 0.17436575674477695 2306 14-15 0.19551300066552812 2306 16-17 0.32109179075230543 2306 18-19 0.4375021335215692 2306 20-21 0.48843748381822394 2306 22-23 0.4855016330070896 2306 24-25 0.4169155311764854 2306 26-27 0.4780025858866921 2306 28-29 0.23267094410645228 2306 30-31 0.22390543946116992 2306 32-33 0.2567767538704331 2306 34-35 0.2521458977811193 2306 36-37 0.24189264017457646 2306 38-39 0.19273261822595344 2306 40-41 0.15362396223424213 2306 42-43 0.00962666610966778 2306 44-45 0.14998009538334145 2306 46-47 0.10977943739986529 2306 48-49 0.08171885393729639 2306 50-51 0.017682619475344552 2306 52-53 0.07656629209482446 2306 54-55 0.2647859371395995 2306 56-57 0.176679667716499 2306 58-59 0.20862411404953463 2306 60-61 0.36182103269351984 2306 62-63 0.30967969625007186 2306 64-65 0.20026789910345855 2306 66-67 0.21420433850959597 2306 68-69 0.3745284418820347 2306 70-71 0.3512792282965975 2306 72-73 0.29312826550814464 2306 74-75 0.20769318643290546 2306 76-77 0.2966782053731052 2306 78-79 0.23768355633196592 2306 80-81 0.2541737087346121 2306 82-83 0.2519667123781488 2306 84-85 0.19534462664331542 2306 86-87 0.23483862026943925 2306 88-89 0.3879192349616787 2306 90-91 0.44717781952281754 2306 92-93 0.5213265309730488 2306 94-95 0.521801902384091 2306 96-97 0.6352518306758235 2306 98-99 0.5829901815723169 2306 100-101 0.25848821537650224 2307 1 0.14584706249399915 2307 2 0.08175089539121672 2307 3 0.066094992740247 2307 4 0.15059715660076023 2307 5 0.09166552883976209 2307 6 0.113536574220241 2307 7 0.2040600526445573 2307 8 0.16513520981102658 2307 9 0.05770384363754033 2307 10-11 0.09444069357530083 2307 12-13 0.09834166063622973 2307 14-15 0.18003565624584894 2307 16-17 0.0979175283078888 2307 18-19 0.29549492973089997 2307 20-21 0.4318036174425899 2307 22-23 0.24008181598627232 2307 24-25 0.2030589423890632 2307 26-27 0.35961125561886575 2307 28-29 0.25777023188015846 2307 30-31 0.19614120541348967 2307 32-33 0.1311436102790111 2307 34-35 0.0505123559244538 2307 36-37 -0.021550978323389813 2307 38-39 -0.08513379066477 2307 40-41 -0.09762623163710771 2307 42-43 -0.16478167920964637 2307 44-45 -0.0831176389436763 2307 46-47 -0.051390125443894874 2307 48-49 0.02850295933398428 2307 50-51 -0.10790631172186949 2307 52-53 -0.10475085879482293 2307 54-55 -0.027298824033003655 2307 56-57 0.07972542418437456 2307 58-59 0.04728557143506151 2307 60-61 0.09488083278629489 2307 62-63 -0.01064369796019804 2307 64-65 0.021625951763255102 2307 66-67 -0.07242691226080211 2307 68-69 0.009726583263727662 2307 70-71 -0.11490005862114572 2307 72-73 -8.329274353968685E-5 2307 74-75 0.05534549945735989 2307 76-77 0.2635084755833432 2307 78-79 0.265501729500599 2307 80-81 0.3030116920622916 2307 82-83 0.18006064709199876 2307 84-85 0.12372249335285446 2307 86-87 0.31676542470546565 2307 88-89 0.43556861228315924 2307 90-91 0.4703585546617539 2307 92-93 0.3152364682038282 2307 94-95 0.23211955428859454 2307 96-97 0.4933434857986363 2307 98-99 0.6222002970851541 2307 100-101 0.44060357683139273 2201 1 0.243361049866742 2201 2 0.2665055268262435 2201 3 0.30448127085706034 2201 4 0.3375321575977921 2201 5 0.2837643391389335 2201 6 0.420383904454269 2201 7 0.3183435745292371 2201 8 0.3881457565725128 2201 9 0.28208301254156964 2201 10-11 0.38850704282508275 2201 12-13 0.33977994571389303 2201 14-15 0.4706565451900957 2201 16-17 0.5382267851889395 2201 18-19 0.7132020146474076 2201 20-21 0.8500394185833571 2201 22-23 0.6924515876080903 2201 24-25 0.6785695555059235 2201 26-27 0.6973679403273891 2201 28-29 0.6010346679173111 2201 30-31 0.5380450159814885 2201 32-33 0.5015418650305676 2201 34-35 0.534194378245715 2201 36-37 0.5456069563162274 2201 38-39 0.4743367036521491 2201 40-41 0.5753962590918533 2201 42-43 0.5311098511903012 2201 44-45 0.5872506367897898 2201 46-47 0.599443298064692 2201 48-49 0.539074391405471 2201 50-51 0.4791328842279441 2201 52-53 0.7200324143422776 2201 54-55 0.6858404249330405 2201 56-57 0.6807565016391308 2201 58-59 0.7457983943560507 2201 60-61 0.7150334875797668 2201 62-63 0.7351560918804267 2201 64-65 0.7842912361964167 2201 66-67 0.8316259379237394 2201 68-69 0.7936010021437667 2201 70-71 0.7241686760192891 2201 72-73 0.768910507963021 2201 74-75 0.7986286340988826 2201 76-77 0.8044567177103588 2201 78-79 0.7997677301634738 2201 80-81 0.8329870520997531 2201 82-83 0.8819371770938176 2201 84-85 0.8566836240756679 2201 86-87 0.9603388045624257 2201 88-89 0.9342889743997524 2201 90-91 1.1274653684399816 2201 92-93 1.2215411475848654 2201 94-95 1.0627590295434821 2201 96-97 1.1550600414987002 2201 98-99 1.264745764128314 2201 100-101 1.1289359539667636 1101 1 0.2142981438035605 1101 2 0.16927379436786794 1101 3 0.0407161516304555 1101 4 0.2505472009132461 1101 5 0.21815559319111344 1101 6 0.23062324277950097 1101 7 0.20931703205771868 1101 8 0.20247999428320185 1101 9 0.21174011542791504 1101 10-11 0.18697016475866945 1101 12-13 0.18959818922210303 1101 14-15 0.21049274921467287 1101 16-17 0.31033475481464734 1101 18-19 0.410597125108886 1101 20-21 0.4623382847719668 1101 22-23 0.3520681471795939 1101 24-25 0.3043822965315002 1101 26-27 0.4223885623439614 1101 28-29 0.26285956579069136 1101 30-31 0.28405291934628707 1101 32-33 0.23127819825776896 1101 34-35 0.3451276735517652 1101 36-37 0.29745986448156003 1101 38-39 0.3962436470328825 1101 40-41 0.2361926684637794 1101 42-43 0.20178193223759422 1101 44-45 0.3059174649085179 1101 46-47 0.2864004718833968 1101 48-49 0.36549847841255456 1101 50-51 0.3607068819241732 1101 52-53 0.47982971620802317 1101 54-55 0.4970122103263819 1101 56-57 0.4817028708734199 1101 58-59 0.5641906515417858 1101 60-61 0.4518745818804675 1101 62-63 0.4616600546267975 1101 64-65 0.4366201718053233 1101 66-67 0.4631904083097851 1101 68-69 0.6270036901974976 1101 70-71 0.6090939819074137 1101 72-73 0.6210663231258238 1101 74-75 0.6613794328562719 1101 76-77 0.704168255344463 1101 78-79 0.6770020380689274 1101 80-81 0.5011749638485838 1101 82-83 0.49646005554623684 1101 84-85 0.6371000228507633 1101 86-87 0.8177092853904 1101 88-89 0.6538642776448178 1101 90-91 0.6732362569139063 1101 92-93 0.8153774696251617 1101 94-95 0.8580844394329539 1101 96-97 0.920317875099002 1101 98-99 0.9401527646729058 1101 100-101 0.6307267809495798 2304 1 0.19284102979252538 2304 2 0.1271239035857903 2304 3 0.22735563349479548 2304 4 0.28104081431352057 2304 5 0.27657010710005636 2304 6 0.2218540731638683 2304 7 0.28264284573263154 2304 8 0.2834134108814226 2304 9 0.2583525846137249 2304 10-11 0.3483861142876705 2304 12-13 0.32134927454332995 2304 14-15 0.4229075008696128 2304 16-17 0.3418732134567932 2304 18-19 0.4748032014016843 2304 20-21 0.6603789511707774 2304 22-23 0.7315835057322886 2304 24-25 0.637508812363933 2304 26-27 0.7213830635265026 2304 28-29 0.5574080285907144 2304 30-31 0.5473254122959332 2304 32-33 0.5489957344992433 2304 34-35 0.5627294027504632 2304 36-37 0.619788704845881 2304 38-39 0.5937499228279179 2304 40-41 0.5525373829453599 2304 42-43 0.45819569525647097 2304 44-45 0.4688479693872267 2304 46-47 0.47608466236635394 2304 48-49 0.5260733073510906 2304 50-51 0.4833768232460969 2304 52-53 0.5396056217809146 2304 54-55 0.7161802660858925 2304 56-57 0.8390364473316154 2304 58-59 0.7927442952681147 2304 60-61 0.7363790831867263 2304 62-63 0.6744931477936476 2304 64-65 0.734719763710558 2304 66-67 0.7790507556663684 2304 68-69 0.7803568500408034 2304 70-71 0.8662049807794645 2304 72-73 0.8410982251959993 2304 74-75 0.8953120797383818 2304 76-77 0.8263471059555272 2304 78-79 1.0038262679359562 2304 80-81 0.9136945227598403 2304 82-83 0.8390899565696799 2304 84-85 0.8250032699124255 2304 86-87 0.918069562014864 2304 88-89 1.0642218002885109 2304 90-91 1.0116050181658345 2304 92-93 0.9364805464994106 2304 94-95 1.0543558614498458 2304 96-97 0.9854870378296816 2304 98-99 1.079154430508538 2304 100-101 0.9605104643665818 2202 1 0.08318749020103411 2202 2 0.11447950166522247 2202 3 0.09444595107667908 2202 4 0.14705823520831984 2202 5 0.11740329715721742 2202 6 0.08075010973539065 2202 7 0.15993033366579823 2202 8 0.17935136373649385 2202 9 0.0661741986998905 2202 10-11 0.12530826657127392 2202 12-13 0.1616696475228636 2202 14-15 0.1636780959830446 2202 16-17 0.19099986141196723 2202 18-19 0.38570588598792455 2202 20-21 0.5280326976753926 2202 22-23 0.4860364177368979 2202 24-25 0.25257056253642673 2202 26-27 0.41289344751410084 2202 28-29 0.3719738571103335 2202 30-31 0.2611211964780935 2202 32-33 0.2492681080980219 2202 34-35 0.11760808690203817 2202 36-37 0.238455990763363 2202 38-39 0.1905125138268957 2202 40-41 0.1945218436203291 2202 42-43 0.15194025727422655 2202 44-45 0.21015910644931068 2202 46-47 0.18597775228648317 2202 48-49 0.19703174683903768 2202 50-51 0.22404712503411162 2202 52-53 0.3034042342720653 2202 54-55 0.4580165010409587 2202 56-57 0.41455676314952683 2202 58-59 0.2961663630745974 2202 60-61 0.5200703836804124 2202 62-63 0.43071896296067536 2202 64-65 0.3875787003110318 2202 66-67 0.48449186394668686 2202 68-69 0.3659141273914841 2202 70-71 0.42532705878273447 2202 72-73 0.5861330543326417 2202 74-75 0.5367466948166992 2202 76-77 0.5684153738916606 2202 78-79 0.6704198079293633 2202 80-81 0.6494729664307144 2202 82-83 0.5476980266283817 2202 84-85 0.6091772436642238 2202 86-87 0.5930799262787261 2202 88-89 0.666642125707785 2202 90-91 0.663368543251071 2202 92-93 0.7415129269159024 2202 94-95 0.8509740226173115 2202 96-97 0.9023801552886077 2202 98-99 0.8692083097948213 2202 100-101 0.667337071906708 1102 1 0.0102630995181201 1102 2 0.04009316212626146 1102 3 0.058836492822031516 1102 4 0.09739876648515633 1102 5 0.048865549992960666 1102 6 0.1235333752767005 1102 7 0.11979966109987572 1102 8 0.04346139027380502 1102 9 -0.026364966580935345 1102 10-11 0.0751377817714527 1102 12-13 0.10001738232185176 1102 14-15 0.07619256490487203 1102 16-17 0.07002379281228599 1102 18-19 0.1692367548996927 1102 20-21 0.3012751057821532 1102 22-23 0.08639218784671954 1102 24-25 -0.002381147383587745 1102 26-27 0.003524428556815451 1102 28-29 -0.032614995532483704 1102 30-31 0.033141367068772354 1102 32-33 0.16263298018005656 1102 34-35 0.0848382773015004 1102 36-37 0.16213328909189073 1102 38-39 0.151343020547408 1102 40-41 0.17738643910359997 1102 42-43 -0.05231776981094782 1102 44-45 0.05118790812828422 1102 46-47 -0.0764779870724368 1102 48-49 -0.02521305564872023 1102 50-51 -0.08180923191594758 1102 52-53 0.07895096796455192 1102 54-55 0.21516039421953082 1102 56-57 0.23853315701888533 1102 58-59 0.15352240448915921 1102 60-61 0.3011488080545419 1102 62-63 0.23673391306986247 1102 64-65 0.2044045123097149 1102 66-67 0.32990377979189844 1102 68-69 0.20266592317723564 1102 70-71 0.26069847929357337 1102 72-73 0.3019400902902518 1102 74-75 0.2653317512425062 1102 76-77 0.37173429722057705 1102 78-79 0.3137867145122968 1102 80-81 0.29232310750803947 1102 82-83 0.26338745545506015 1102 84-85 0.3541383245841949 1102 86-87 0.3609157649399606 1102 88-89 0.2802649592973445 1102 90-91 0.2887708182716473 1102 92-93 0.3536008337491978 1102 94-95 0.4270995359530865 1102 96-97 0.4139760703933355 1102 98-99 0.3040905051340026 1102 100-101 0.3217838681142666 2305 1 0.060564031102714466 2305 2 0.13714258515479116 2305 3 0.12440111999687531 2305 4 0.18856794193230542 2305 5 0.1476511858248699 2305 6 0.13388315265677164 2305 7 0.18312029092915338 2305 8 0.11914939473081887 2305 9 0.0668344237903824 2305 10-11 0.16427156186627911 2305 12-13 0.1493470605639189 2305 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-1.1208880895007027 1213 52-53 -0.9902357431057851 1213 54-55 -1.035862190177582 1213 56-57 -0.8863829654700837 1213 58-59 -1.0122728920448765 1213 60-61 -1.0364328738622177 1213 62-63 -1.0736679128484 1213 64-65 -1.0437928899090423 1213 66-67 -1.0041655747450449 1213 68-69 -0.951774544746911 1213 70-71 -1.8439721243190021 1213 72-73 -1.30274116893316 1213 74-75 -1.2456666528530604 1213 76-77 -1.274650018808515 1213 78-79 -1.1767974096364497 1213 80-81 -1.268006920564396 1213 82-83 -1.2863263252027082 1213 84-85 -1.4452580407302769 1213 86-87 -1.375722113251328 1213 88-89 -1.5780090970052036 1213 90-91 -1.6259697853358368 1213 92-93 -1.6489121333640178 1213 94-95 -1.9199999586025491 1213 96-97 -1.8866182576363535 1213 98-99 -1.8698912478125997 1213 100-101 -1.6752811345691896 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 373.0 3 124.0 4 112.0 5 149.0 6 567.0 7 1209.0 8 2939.0 9 4562.0 10 4688.0 11 6189.0 12 6508.0 13 8576.0 14 12267.0 15 15860.0 16 17377.0 17 16993.0 18 17052.0 19 17996.0 20 19754.0 21 21614.0 22 23643.0 23 26019.0 24 28673.0 25 31956.0 26 35802.0 27 41156.0 28 47696.0 29 55783.0 30 66898.0 31 81745.0 32 105151.0 33 139575.0 34 201889.0 35 328132.0 36 653633.0 37 1032988.0 38 368295.0 39 203.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 32.79763615608305 32.626724678628236 19.331295389724716 15.244343775563992 2 30.202610249715516 26.404286189668376 30.44747387306884 12.94562968754727 3 33.811375952564084 24.325211668791958 24.84618661750144 17.01722576114251 4 29.27032746037271 24.270765090007767 29.251397434298127 17.207510015321397 5 29.052197462666555 22.684698501544283 30.49060328189398 17.772500753895173 6 26.648057556634125 23.336853189996088 31.99383986319077 18.021249390179015 7 30.704328025638777 23.38334863187977 26.001144048361624 19.911179294119837 8 27.1384799238099 28.588434252181212 25.10858455150984 19.164501272499056 9 24.985112772383953 26.53010039033744 29.040541793366735 19.444245043911874 10-11 20.141229866462883 31.649291998305994 32.95371783519211 15.25576030003901 12-13 20.29908701908804 30.945212571873142 32.966037819197844 15.789662589840974 14-15 20.355159254921222 30.905583440627083 33.11716186484766 15.622095439604037 16-17 20.01918635833067 30.98415236444549 33.099781123814346 15.896880153409496 18-19 19.348102822753777 31.40757290020373 32.68006354316342 16.564260733879074 20-21 19.36305375251059 31.685288969346033 32.18658656599159 16.765070712151783 22-23 19.40706594284145 31.101309614033358 32.26611984970284 17.225504593422354 24-25 19.30937802869515 30.850976021848748 32.42578812790875 17.41385782154735 26-27 19.327803646366963 31.25754830807973 32.27087613589524 17.143771909658064 28-29 19.102470427959744 31.529769422169533 32.057671564926075 17.310088584944644 30-31 18.596034923218795 32.568014170425144 31.847678405611834 16.988272500744227 32-33 18.29189131286528 32.99172847893784 31.962780483914255 16.75359972428263 34-35 18.685687626529266 32.451707875868316 32.37261799017685 16.489986507425574 36-37 18.67102768404918 32.0239658045479 32.837436412137684 16.46757009926524 38-39 19.015392497403777 31.880524935758466 32.17527550539949 16.928807061438267 40-41 18.88806734822198 31.847636873850167 31.667593325725996 17.59670245220186 42-43 19.179627389235137 31.435848608860606 31.582758635750192 17.80176536615406 44-45 18.823993973066266 31.390983353157242 31.821025335594598 17.963997338181894 46-47 18.92708971596933 31.618627026666623 31.728304642315443 17.7259786150486 48-49 18.914160122846727 32.19352725843662 31.159897239587504 17.732415379129158 50-51 19.469647882331344 32.06205327657471 30.309915547718457 18.15838329337549 52-53 19.567363668575343 31.90979726395283 30.41375529418752 18.109083773284308 54-55 19.98357722741858 31.939512850266492 30.310508871630066 17.766401050684863 56-57 20.108323985605324 32.011487443087546 30.150826185827107 17.729362385480023 58-59 19.91422509522904 32.56531035911146 30.04718260177765 17.473281943881858 60-61 19.734977221148974 32.43928695531713 29.86891756366699 17.956818259866907 62-63 19.49645168926887 32.339760737654025 30.27945175023466 17.88433582284244 64-65 19.414348722564466 31.909719963921507 30.695730251151172 17.980201062362855 66-67 19.866095107077207 31.553895984555723 30.367083801949047 18.212925106418027 68-69 20.26623837091617 31.57739642692029 30.47587947822774 17.6804857239358 70-71 20.006338026324634 31.84063792322258 30.51410472759145 17.63891932286133 72-73 20.083572251835864 31.798745703322435 30.18527835254332 17.932403692298383 74-75 20.46677791789671 31.397291295908047 29.82004393303217 18.31588685316307 76-77 20.605577976771016 31.204778553778006 29.373214950091718 18.816428519359256 78-79 20.439964723433846 31.31821066151419 29.657779185632815 18.58404542941915 80-81 20.26284771334276 31.702449345463645 29.270158497331217 18.764544443862373 82-83 19.97638829393852 31.785559946907867 29.14198623324878 19.09606552590483 84-85 20.08344706502377 31.79473774372142 29.63871382593601 18.483101365318806 86-87 20.45448591324213 31.505850634315998 29.92161797590583 18.118045476536043 88-89 20.949364619913 31.335447967994632 29.764404626619594 17.950782785472768 90-91 20.986784735910103 31.307302332783426 29.301946256451334 18.40396667485514 92-93 20.750384218684047 31.430827032990795 29.218763344715487 18.600025403609667 94-95 20.68389809547366 31.234639391249946 29.246962796991166 18.834499716285226 96-97 20.803132418077077 31.646844391111724 28.993038682971427 18.556984507839775 98-99 21.435794540317072 31.38264827902944 28.65561539924599 18.525941781407496 100-101 21.867328947397144 31.284250724581785 28.127861124795434 18.720559203225633 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 148690.0 1 83780.5 2 13798.5 3 8645.0 4 7727.0 5 6671.0 6 6918.0 7 7747.0 8 8175.0 9 8091.0 10 7596.0 11 7044.0 12 6682.5 13 6439.0 14 6238.5 15 6103.5 16 6147.0 17 6181.0 18 6140.0 19 6174.5 20 6405.5 21 6759.5 22 7248.5 23 8010.0 24 9097.0 25 10603.0 26 12765.5 27 15904.5 28 19533.5 29 23259.0 30 28062.5 31 33994.0 32 42834.0 33 57920.5 34 78041.5 35 99772.0 36 128697.0 37 166953.5 38 196312.0 39 200594.5 40 199348.0 41 198345.5 42 190844.5 43 186119.5 44 175055.5 45 161030.5 46 156728.0 47 145758.0 48 121041.5 49 97081.5 50 77897.5 51 62824.5 52 50549.5 53 39567.5 54 31597.5 55 26482.5 56 21059.0 57 15479.0 58 11087.0 59 7915.0 60 5685.5 61 4414.5 62 3434.0 63 2588.0 64 2101.5 65 1574.0 66 1089.5 67 793.0 68 603.5 69 503.5 70 415.5 71 330.5 72 278.5 73 222.5 74 165.5 75 135.0 76 118.0 77 90.5 78 71.5 79 56.0 80 48.0 81 44.5 82 34.0 83 25.0 84 21.5 85 19.5 86 11.5 87 8.5 88 8.5 89 7.5 90 6.0 91 4.0 92 4.5 93 3.0 94 2.0 95 2.5 96 1.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19435877573134241 2 0.1833255616922163 3 0.15681681322452648 4 0.14996460661075342 5 0.15501665724972172 6 0.13390837670644623 7 0.10835777577373318 8 0.09520502324814337 9 0.09514695370056903 10-11 0.07530168581703563 12-13 0.09507436676610109 14-15 0.09976348273272968 16-17 0.09173536778057609 18-19 0.08769953422415891 20-21 0.08642200417752326 22-23 0.10535267668676067 24-25 0.4495454025468142 26-27 0.29079777686544067 28-29 0.11946357674732722 30-31 0.22336451474472915 32-33 0.253880061995049 34-35 0.2375625191266572 36-37 0.23955140113107865 38-39 0.2574948913315521 40-41 0.25742230439708425 42-43 0.2825373837229897 44-45 0.2584094867058481 46-47 0.27683205067381 48-49 0.2604709556447375 50-51 0.2753803119844513 52-53 0.27533675982377054 54-55 0.25180407566926605 56-57 0.2690071791381666 58-59 0.25769813474806236 60-61 0.23413641581977068 62-63 0.22625347473655297 64-65 0.2250049794637045 66-67 0.23374444637364386 68-69 0.2381577319892943 70-71 0.2248743229816622 72-73 0.21715107315427393 74-75 0.22587602267731974 76-77 0.22625347473655297 78-79 0.22851818709195257 80-81 0.2359365717945755 82-83 0.2608919598646515 84-85 0.25534631807130126 86-87 0.2552737311368333 88-89 0.25324129697173114 90-91 0.24453086483557898 92-93 0.22143370228788212 94-95 0.2205481416873733 96-97 0.21601871697657415 98-99 0.23955140113107865 100-101 0.2480585898507206 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3444146.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.57594766304875 #Duplication Level Percentage of deduplicated Percentage of total 1 82.19644042534762 36.639842264891996 2 8.628130839198024 7.6921421743605585 3 2.964753635587331 3.964701086813231 4 1.5876717749225389 2.8308789578018723 5 1.0865913947719825 2.4217920572237515 6 0.5000762442646097 1.3374823495123933 7 0.4710519632760442 1.4698311363098513 8 0.3607244688817529 1.2863708036523258 9 0.24486901877802197 0.9823741708816086 >10 1.6822723085283293 14.221579324038963 >50 0.14556689024318678 4.5248319807990525 >100 0.120233164314247 11.01602152803063 >500 0.009556268660272444 2.8412025236137235 >1k 0.0019384538884332943 1.1077745308480487 >5k 0.0 0.0 >10k+ 1.2314933745938148E-4 7.663175111221972 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 155994 4.529250502156413 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 123200 3.57708413057983 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.1613909514869579E-4 0.0 0.0 2 1.7420864272304367E-4 0.0 0.0 3 2.3227819029739157E-4 0.0 0.0 4 2.9034773787173945E-4 0.0 0.0 5 3.4841728544608735E-4 0.0 0.0 6 4.6455638059478315E-4 0.0 0.0 7 5.22625928169131E-4 0.0 0.0 8 7.549041184665227E-4 0.0 0.0 9 9.291127611895663E-4 0.0 0.0 10-11 0.0022937471291867417 0.0 0.0 12-13 0.007549041184665227 0.0 0.0 14-15 0.016273990707710996 0.0 0.0 16-17 0.029571917102236663 0.0 0.0 18-19 0.041360035259829286 0.0 0.0 20-21 0.04834289835564462 0.0 0.0 22-23 0.055325761451459954 0.0 0.0 24-25 0.06335387640361355 0.0 0.0 26-27 0.07287728220580661 0.0 0.0 28-29 0.08404115272697499 0.0 0.0 30-31 0.09574216656320608 0.0 0.0 32-33 0.1098821013975598 0.0 0.0 34-35 0.12487856205863515 0.0 0.0 36-37 0.14105093105809102 0.0 0.0 38-39 0.1587185909075864 0.0 0.0 40-41 0.17793961115469553 2.9034773787173947E-5 0.0 42-43 0.2002673521970323 2.9034773787173947E-5 0.0 44-45 0.224671079565152 2.9034773787173947E-5 0.0 46-47 0.24972808934348312 2.9034773787173947E-5 0.0 48-49 0.2771659505723625 2.9034773787173947E-5 0.0 50-51 0.30495222908668795 2.9034773787173947E-5 0.0 52-53 0.33588878055692184 2.9034773787173947E-5 0.0 54-55 0.3670140580567723 2.9034773787173947E-5 0.0 56-57 0.39928620912121615 2.9034773787173947E-5 1.4517386893586973E-5 58-59 0.43475218530224913 2.9034773787173947E-5 2.9034773787173947E-5 60-61 0.47165538278574715 2.9034773787173947E-5 2.9034773787173947E-5 62-63 0.5123476182484714 2.9034773787173947E-5 2.9034773787173947E-5 64-65 0.5552755312928082 5.8069547574347894E-5 2.9034773787173947E-5 66-67 0.6009617478469264 5.8069547574347894E-5 2.9034773787173947E-5 68-69 0.651061250016695 5.8069547574347894E-5 2.9034773787173947E-5 70-71 0.7067063939798138 5.8069547574347894E-5 2.9034773787173947E-5 72-73 0.7676068319984113 5.8069547574347894E-5 2.9034773787173947E-5 74-75 0.8346626420598895 5.8069547574347894E-5 2.9034773787173947E-5 76-77 0.9105159885788814 5.8069547574347894E-5 2.9034773787173947E-5 78-79 0.9955878757753011 5.8069547574347894E-5 2.9034773787173947E-5 80-81 1.093333441729822 5.8069547574347894E-5 2.9034773787173947E-5 82-83 1.2078320721595426 5.8069547574347894E-5 2.9034773787173947E-5 84-85 1.341029096908203 5.8069547574347894E-5 2.9034773787173947E-5 86-87 1.488511230360153 5.8069547574347894E-5 2.9034773787173947E-5 88-89 1.6553885927019354 5.8069547574347894E-5 2.9034773787173947E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CACGCCG 60 1.500217E-6 47.4675 1 AACGCGC 60 1.500217E-6 47.4675 1 CTATGCG 75 6.9589078E-6 37.974003 1 CGCGAGG 50 0.0016217066 37.974003 1 CGGACGG 75 6.9589078E-6 37.974003 1 CGTGGCT 190 0.0 37.474346 1 GTCGACC 70 2.0624539E-4 33.90536 1 TGCCGGC 70 2.0645691E-4 33.899445 2 CGCGGAG 155 3.6379788E-11 33.686615 1 CGAGGCG 155 3.6379788E-11 33.686615 1 CGGTCAG 85 1.6408116E-5 33.506474 1 GCGCAAC 85 1.6427723E-5 33.500626 2 CGCGTGG 60 0.0039652996 31.645 1 TGCGACC 60 0.003968688 31.63948 2 TTGACCG 435 0.0 30.544466 5 CGGGCGA 125 2.1689993E-7 30.379202 1 GTTTGAT 14350 0.0 30.191797 3 CGGGGTG 780 0.0 29.819328 1 GTGTTTG 50000 0.0 29.306435 1 CGTGCGG 65 0.0058652507 29.210772 1 >>END_MODULE