FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n02_50220371-2.34200000035902.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n02_50220371-2.34200000035902.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3444146
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1559944.529250502156413No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1232003.57708413057983No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCCG601.500217E-647.46751
AACGCGC601.500217E-647.46751
CTATGCG756.9589078E-637.9740031
CGCGAGG500.001621706637.9740031
CGGACGG756.9589078E-637.9740031
CGTGGCT1900.037.4743461
GTCGACC702.0624539E-433.905361
TGCCGGC702.0645691E-433.8994452
CGCGGAG1553.6379788E-1133.6866151
CGAGGCG1553.6379788E-1133.6866151
CGGTCAG851.6408116E-533.5064741
GCGCAAC851.6427723E-533.5006262
CGCGTGG600.003965299631.6451
TGCGACC600.00396868831.639482
TTGACCG4350.030.5444665
CGGGCGA1252.1689993E-730.3792021
GTTTGAT143500.030.1917973
CGGGGTG7800.029.8193281
GTGTTTG500000.029.3064351
CGTGCGG650.005865250729.2107721
Produced by FastQC (version 0.11.2)