FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n02_50110001-2.342000000358f4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n02_50110001-2.342000000358f4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6097714
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC209720.34393216867829485No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA117130.19208837935003184No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106900.17531160038007687No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG92440.1515977955017241No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA79700.13070471983435106No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTGG3000.053.7848631
CGCGGAG4700.048.4669651
CGGACGG1300.047.4572331
CGAGGGG10800.046.1389771
CGACGGG2750.044.8686561
CCGAGTG1006.4392225E-1042.711511
CCGGTTG1850.041.0221866
CGTTTGA6600.040.985791
CGAACGG704.340036E-640.6776281
CGGGAGA9750.039.9009484
CTAGTCG608.339097E-539.5476951
ATTACGG2050.039.354781
GTTACGG3400.039.0824281
CACGGGG5150.038.7029881
CGGATGG1600.038.5590021
CGGGCGA1251.364242E-1037.9657861
GGACGGG9950.036.2487451
TAGACCG2100.036.1525422
GAGCGGC7750.034.873379
ATGACCG1502.5465852E-1134.8019681
Produced by FastQC (version 0.11.2)