##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n02_44065-1.342000000357db.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3956237 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.289282467152496 33.0 33.0 33.0 33.0 33.0 2 32.28608346769923 33.0 33.0 33.0 33.0 33.0 3 32.27037662303851 33.0 33.0 33.0 33.0 33.0 4 36.08594151462614 37.0 37.0 37.0 37.0 37.0 5 36.08753216756226 37.0 37.0 37.0 37.0 37.0 6 36.058435579061616 37.0 37.0 37.0 33.0 37.0 7 36.01316907960772 37.0 37.0 37.0 33.0 37.0 8 35.97520042403931 37.0 37.0 37.0 33.0 37.0 9 35.96920002517544 37.0 37.0 37.0 33.0 37.0 10-11 35.901566690772064 37.0 37.0 37.0 33.0 37.0 12-13 35.91233361398723 37.0 37.0 37.0 33.0 37.0 14-15 37.7962394568374 40.0 37.0 40.0 33.0 40.0 16-17 37.762518524547446 40.0 37.0 40.0 33.0 40.0 18-19 37.704697544661755 40.0 37.0 40.0 33.0 40.0 20-21 37.611899261849075 40.0 37.0 40.0 33.0 40.0 22-23 37.64185398903049 40.0 37.0 40.0 33.0 40.0 24-25 37.475337802057865 40.0 37.0 40.0 33.0 40.0 26-27 37.438671520437225 40.0 37.0 40.0 33.0 40.0 28-29 37.44683003065792 40.0 37.0 40.0 33.0 40.0 30-31 37.40947749085811 40.0 37.0 40.0 33.0 40.0 32-33 37.35109688828045 40.0 37.0 40.0 33.0 40.0 34-35 37.301044907066995 40.0 37.0 40.0 33.0 40.0 36-37 37.2227308424647 40.0 37.0 40.0 33.0 40.0 38-39 37.185536154684364 40.0 37.0 40.0 33.0 40.0 40-41 37.08978468681224 40.0 37.0 40.0 33.0 40.0 42-43 37.01568485406713 40.0 37.0 40.0 33.0 40.0 44-45 36.87407541560326 40.0 37.0 40.0 33.0 40.0 46-47 36.79419622737465 40.0 37.0 40.0 33.0 40.0 48-49 36.66565299298298 40.0 37.0 40.0 33.0 40.0 50-51 35.4500529164456 37.0 35.0 38.5 30.0 38.5 52-53 35.841386524619224 37.0 37.0 38.5 33.0 40.0 54-55 36.647114796206594 37.0 37.0 40.0 33.0 40.0 56-57 36.66331819858112 37.0 37.0 40.0 33.0 40.0 58-59 36.50924603354147 37.0 37.0 40.0 33.0 40.0 60-61 36.24190145838078 37.0 37.0 40.0 33.0 40.0 62-63 35.99561666806109 37.0 37.0 40.0 33.0 40.0 64-65 35.750066793268445 37.0 37.0 40.0 33.0 40.0 66-67 35.51302930031744 37.0 37.0 38.5 33.0 40.0 68-69 35.28389932150172 37.0 35.0 37.0 33.0 40.0 70-71 34.93096495482955 37.0 33.0 37.0 27.0 40.0 72-73 34.66769293649496 37.0 33.0 37.0 27.0 40.0 74-75 34.413037692130175 37.0 33.0 37.0 27.0 37.0 76-77 34.192554439989316 37.0 33.0 37.0 27.0 37.0 78-79 33.98316013423867 37.0 33.0 37.0 27.0 37.0 80-81 33.78307151467417 37.0 33.0 37.0 27.0 37.0 82-83 33.5945370562987 37.0 33.0 37.0 27.0 37.0 84-85 33.431753330247915 37.0 33.0 37.0 27.0 37.0 86-87 33.29668647252427 37.0 33.0 37.0 27.0 37.0 88-89 33.15562591422102 37.0 33.0 37.0 27.0 37.0 90-91 33.00711800127242 37.0 33.0 37.0 27.0 37.0 92-93 32.882521825664135 37.0 33.0 37.0 27.0 37.0 94-95 32.76790419785266 37.0 33.0 37.0 27.0 37.0 96-97 32.637352236481284 33.0 33.0 37.0 27.0 37.0 98-99 32.495249399871646 33.0 33.0 37.0 24.5 37.0 100-101 31.187762133562778 33.0 30.0 37.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.21313149118396524 2306 2 0.22045333099396203 2306 3 0.19876677158778477 2306 4 0.29827853315745045 2306 5 0.24552793071609358 2306 6 0.22304529536046402 2306 7 0.28689597271884537 2306 8 0.3353274774545625 2306 9 0.3396761860605366 2306 10-11 0.3216515204085084 2306 12-13 0.26340409964895173 2306 14-15 0.4638065822538664 2306 16-17 0.4023928303784743 2306 18-19 0.4022193437112094 2306 20-21 0.4656590614979663 2306 22-23 0.33211027132161774 2306 24-25 0.34379264155976585 2306 26-27 0.3898702112518251 2306 28-29 0.30072700106580186 2306 30-31 0.2381832837369089 2306 32-33 0.1605812362613932 2306 34-35 0.20260404714024816 2306 36-37 0.17422571961167677 2306 38-39 0.02101221615219373 2306 40-41 0.1430118734103445 2306 42-43 0.04768140112990693 2306 44-45 0.07381790513563402 2306 46-47 0.10093072028180927 2306 48-49 0.06624568924232221 2306 50-51 0.07176002435942763 2306 52-53 0.13032892347419534 2306 54-55 0.2808057646253488 2306 56-57 0.0957679014233932 2306 58-59 0.25554078334370445 2306 60-61 0.3600320691012584 2306 62-63 0.20296148882071918 2306 64-65 0.0805902400821239 2306 66-67 0.1274007284083325 2306 68-69 0.0829986241796945 2306 70-71 0.1824088092520526 2306 72-73 0.1801991188180878 2306 74-75 0.16410881746536177 2306 76-77 0.10363437064874859 2306 78-79 0.09188227552233741 2306 80-81 0.18697331188233335 2306 82-83 0.1397957397072318 2306 84-85 0.15526888755644563 2306 86-87 0.16403584592845277 2306 88-89 0.38224929209370373 2306 90-91 0.23469966397738773 2306 92-93 0.2359105449426977 2306 94-95 0.2202743406257568 2306 96-97 0.24194418624240654 2306 98-99 0.09719986200785513 2306 100-101 0.026871504370657107 2307 1 0.30702137724438217 2307 2 0.2617464950556112 2307 3 0.2351115993245827 2307 4 0.2963612343422639 2307 5 0.35688051018775013 2307 6 0.28703750432102737 2307 7 0.40992866975484077 2307 8 0.3658264053132072 2307 9 0.2828578255852108 2307 10-11 0.3375174110282586 2307 12-13 0.31321631114248305 2307 14-15 0.5520770064375355 2307 16-17 0.4426105231128048 2307 18-19 0.5212181907771267 2307 20-21 0.6368548703205335 2307 22-23 0.5630597278267402 2307 24-25 0.5390147342224694 2307 26-27 0.4990685726879249 2307 28-29 0.49421903752297425 2307 30-31 0.36382984393186035 2307 32-33 0.28852296505111497 2307 34-35 0.2741414236038011 2307 36-37 0.3598777496927781 2307 38-39 0.211531462290516 2307 40-41 0.18214093870396653 2307 42-43 0.13935467314810523 2307 44-45 0.20110013597574294 2307 46-47 0.22458219136456137 2307 48-49 0.20225107833574896 2307 50-51 0.1480167880210317 2307 52-53 0.07270531059801044 2307 54-55 0.27665831352678083 2307 56-57 0.2844255302222862 2307 58-59 0.24530177879747583 2307 60-61 0.3896095231258201 2307 62-63 0.2478422322011795 2307 64-65 0.3357627762314124 2307 66-67 0.24252097856009414 2307 68-69 0.4092263461304242 2307 70-71 0.19892186055325567 2307 72-73 0.1748069683776592 2307 74-75 0.24877261053116229 2307 76-77 0.4000735402741711 2307 78-79 0.3604559235542766 2307 80-81 0.4086944372158783 2307 82-83 0.19774503462689097 2307 84-85 0.09876032352711661 2307 86-87 0.20638450149376553 2307 88-89 0.2219663428533778 2307 90-91 0.3141968711692513 2307 92-93 0.18869287186065264 2307 94-95 0.1743767006468957 2307 96-97 0.34444832414031623 2307 98-99 0.2987689878305204 2307 100-101 0.08133710413658335 2201 1 0.20949369509190063 2201 2 0.1979299961301777 2201 3 0.24589199868278655 2201 4 0.26107413081485475 2201 5 0.2056388498235009 2201 6 0.22303193290052548 2201 7 0.2979254540469398 2201 8 0.2653714720176481 2201 9 0.22591696796754945 2201 10-11 0.20464629354867725 2201 12-13 0.24090567241049143 2201 14-15 0.39761911121699 2201 16-17 0.43888287561307493 2201 18-19 0.3766589889539347 2201 20-21 0.5326991643782151 2201 22-23 0.47532339774706145 2201 24-25 0.38222852820975817 2201 26-27 0.4207560942405024 2201 28-29 0.3232953941444734 2201 30-31 0.304460764303883 2201 32-33 0.30445553720430496 2201 34-35 0.23114123727148694 2201 36-37 0.27306612533872254 2201 38-39 0.3186753357093295 2201 40-41 0.20943372199442933 2201 42-43 0.24885109742257328 2201 44-45 0.33968179084276784 2201 46-47 0.1789911111177247 2201 48-49 0.29848855676208785 2201 50-51 0.19006739621476498 2201 52-53 0.2443349194772111 2201 54-55 0.4238049313423389 2201 56-57 0.3962562726090013 2201 58-59 0.3744194805359271 2201 60-61 0.35490671051963574 2201 62-63 0.3199280179965882 2201 64-65 0.3813374153917195 2201 66-67 0.3800900838192973 2201 68-69 0.35866515704358903 2201 70-71 0.3082526619592585 2201 72-73 0.2816976385247827 2201 74-75 0.31583538091326346 2201 76-77 0.29587420221761107 2201 78-79 0.2868609303288423 2201 80-81 0.2395775216986138 2201 82-83 0.24561963424427802 2201 84-85 0.30402829005138443 2201 86-87 0.3618185862269101 2201 88-89 0.28454512305965807 2201 90-91 0.2538519708991984 2201 92-93 0.2813217425772194 2201 94-95 0.23287888183104144 2201 96-97 0.23635787624296256 2201 98-99 0.21224502979491433 2201 100-101 -0.055314198545509896 1101 1 0.2988001994080349 1101 2 0.35755354410668616 1101 3 0.3021224823420283 1101 4 0.37411397664597956 1101 5 0.3953029115529887 1101 6 0.37372917614767687 1101 7 0.4488398856208633 1101 8 0.3820820328581078 1101 9 0.4105208513993617 1101 10-11 0.38341570874781894 1101 12-13 0.38332585303878375 1101 14-15 0.5168706980516262 1101 16-17 0.5914819201199393 1101 18-19 0.5444621358037338 1101 20-21 0.6409865637677115 1101 22-23 0.4349169323099602 1101 24-25 0.45761649344065347 1101 26-27 0.562608526773964 1101 28-29 0.5054104677596101 1101 30-31 0.45182149543489913 1101 32-33 0.41362359460860887 1101 34-35 0.42844904084613233 1101 36-37 0.37809323177756227 1101 38-39 0.4691903304630287 1101 40-41 0.32263191011726633 1101 42-43 0.41131657981923553 1101 44-45 0.42664384202198136 1101 46-47 0.42917725525741446 1101 48-49 0.42379209051487976 1101 50-51 0.4000475676173991 1101 52-53 0.49244103049277754 1101 54-55 0.5912214171558574 1101 56-57 0.5598467014949762 1101 58-59 0.5613245052614317 1101 60-61 0.6175925074386797 1101 62-63 0.6425033992351672 1101 64-65 0.6753336099502647 1101 66-67 0.529555818924841 1101 68-69 0.5339043908566055 1101 70-71 0.628719893670457 1101 72-73 0.6060301255914737 1101 74-75 0.5227230383155117 1101 76-77 0.524485628394828 1101 78-79 0.6288387591172722 1101 80-81 0.5804448735408911 1101 82-83 0.5090835441548691 1101 84-85 0.5521048328700502 1101 86-87 0.6013888766059594 1101 88-89 0.6562718486543062 1101 90-91 0.7442854251798465 1101 92-93 0.6918673488835054 1101 94-95 0.633246543728049 1101 96-97 0.6120146071889181 1101 98-99 0.6358649571916857 1101 100-101 0.5388953032115005 2304 1 0.2776489739882564 2304 2 0.21860697670434348 2304 3 0.1827566728176535 2304 4 0.28485121297025984 2304 5 0.2499530576541389 2304 6 0.3290819048328757 2304 7 0.27462999202077043 2304 8 0.22318095944621774 2304 9 0.2588823052208724 2304 10-11 0.26704381346741 2304 12-13 0.31836466265443164 2304 14-15 0.4063292908950942 2304 16-17 0.34747223133866356 2304 18-19 0.4329731890506636 2304 20-21 0.49543727102646073 2304 22-23 0.4811503015759584 2304 24-25 0.3454248691121009 2304 26-27 0.3856970766700698 2304 28-29 0.3174982750817392 2304 30-31 0.28715664379554795 2304 32-33 0.2663004231139183 2304 34-35 0.21288704256824076 2304 36-37 0.2941795655234145 2304 38-39 0.3034253052570577 2304 40-41 0.2677724115218041 2304 42-43 0.14808895727617966 2304 44-45 0.301355748102047 2304 46-47 0.34595732874797136 2304 48-49 0.2988138413953436 2304 50-51 0.3837266568325788 2304 52-53 0.4213385572697774 2304 54-55 0.4575509333014196 2304 56-57 0.38395783422383545 2304 58-59 0.4298600248486153 2304 60-61 0.5219103087219494 2304 62-63 0.348282056576096 2304 64-65 0.27169711316887657 2304 66-67 0.2939745909256146 2304 68-69 0.40780399060585637 2304 70-71 0.44837148061901644 2304 72-73 0.42636698941114304 2304 74-75 0.46096029601286403 2304 76-77 0.3723896552632411 2304 78-79 0.3217071732637109 2304 80-81 0.42463119458380305 2304 82-83 0.3675857657887249 2304 84-85 0.41678020265229065 2304 86-87 0.48114271233864514 2304 88-89 0.5132321433557436 2304 90-91 0.3274584604611306 2304 92-93 0.30598444966535965 2304 94-95 0.41693782638967036 2304 96-97 0.3036756369772817 2304 98-99 0.25946548503320344 2304 100-101 0.27907541215977005 2202 1 0.2564593149462766 2202 2 0.18055607630343928 2202 3 0.1954574467574659 2202 4 0.22556128896557937 2202 5 0.29295647353941945 2202 6 0.2767257240139642 2202 7 0.2842658911958651 2202 8 0.2019723140343217 2202 9 0.17511578599128086 2202 10-11 0.2658132198118821 2202 12-13 0.23571260378435 2202 14-15 0.3943846095825023 2202 16-17 0.39038055715457887 2202 18-19 0.35544033017089305 2202 20-21 0.4376845705515535 2202 22-23 0.3557587769174546 2202 24-25 0.23501299699827882 2202 26-27 0.3528876405707919 2202 28-29 0.2890701020617925 2202 30-31 0.22748228535487414 2202 32-33 0.14551103630483908 2202 34-35 0.09722998798867621 2202 36-37 0.24622082575320547 2202 38-39 0.1698208307392477 2202 40-41 0.16886422272418855 2202 42-43 0.1647091904019078 2202 44-45 0.22098462469356406 2202 46-47 0.3065185642606778 2202 48-49 0.23383425609405606 2202 50-51 0.1815824153234189 2202 52-53 0.19887660575733435 2202 54-55 0.3271318574424029 2202 56-57 0.409892028889665 2202 58-59 0.33226735415700404 2202 60-61 0.4266605560787511 2202 62-63 0.4123648523380581 2202 64-65 0.303246012525058 2202 66-67 0.42477647946819275 2202 68-69 0.40571984095750935 2202 70-71 0.41485808529993307 2202 72-73 0.38696275638415045 2202 74-75 0.30618649369088047 2202 76-77 0.2943846290964629 2202 78-79 0.380336608924722 2202 80-81 0.3724620295086254 2202 82-83 0.33899786993180925 2202 84-85 0.4180432313564353 2202 86-87 0.425043606019166 2202 88-89 0.34675435151486766 2202 90-91 0.38004386214191044 2202 92-93 0.4224594607941441 2202 94-95 0.33599347282928704 2202 96-97 0.2874759915002727 2202 98-99 0.27759774519361713 2202 100-101 0.10615752275110424 1102 1 0.011015247231242142 1102 2 0.07125373630699272 1102 3 0.03841315575156301 1102 4 0.07299966064861962 1102 5 0.04766043994224134 1102 6 0.07903520209890047 1102 7 0.09375815969475809 1102 8 0.058496480239440984 1102 9 0.064537216883231 1102 10-11 0.1551632588804921 1102 12-13 0.0746636252744537 1102 14-15 0.08518618516139753 1102 16-17 0.07864641038713671 1102 18-19 0.11798303126850129 1102 20-21 0.20494365322861086 1102 22-23 0.09344771639884186 1102 24-25 -0.0579596119499044 1102 26-27 -0.031336188473865434 1102 28-29 -0.07420029975603626 1102 30-31 -0.032302058833934666 1102 32-33 -0.12000747871606876 1102 34-35 -0.04508837214332573 1102 36-37 -0.016560662398042325 1102 38-39 -0.0621921306322335 1102 40-41 -0.09144786674092131 1102 42-43 -0.08313667618288889 1102 44-45 0.022863199526092615 1102 46-47 0.015553640689674353 1102 48-49 -0.05578034838320889 1102 50-51 -0.18178745375389127 1102 52-53 4.608260496041794E-4 1102 54-55 0.08967605346582985 1102 56-57 0.15909740289276186 1102 58-59 0.1319831724046523 1102 60-61 0.14687807872413572 1102 62-63 0.18099150044818657 1102 64-65 0.12379256469741762 1102 66-67 0.048490633608167855 1102 68-69 -0.014176053866805205 1102 70-71 0.04080817446678964 1102 72-73 0.13245856311721838 1102 74-75 0.1422421355591581 1102 76-77 0.06274984749601487 1102 78-79 -0.04529794150916189 1102 80-81 -0.055586534432812584 1102 82-83 -0.010242835366902625 1102 84-85 0.10874668875524662 1102 86-87 0.1412954550783283 1102 88-89 0.16023402471678594 1102 90-91 0.24546947299230482 1102 92-93 0.24949424625953043 1102 94-95 0.1880701982562627 1102 96-97 0.19098684302096558 1102 98-99 0.13855651769179644 1102 100-101 0.022574834575493696 2305 1 0.13957210657534347 2305 2 0.1713067575627818 2305 3 0.1429304929644033 2305 4 0.21308262331944405 2305 5 0.19683824157566931 2305 6 0.14382596935772085 2305 7 0.17399055444118972 2305 8 0.20644530215140833 2305 9 0.11074116661465183 2305 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8559.0 12 6737.0 13 5982.0 14 5741.0 15 6191.0 16 6632.0 17 6977.0 18 7833.0 19 8648.0 20 9981.0 21 11216.0 22 12957.0 23 13996.0 24 15957.0 25 19018.0 26 23042.0 27 28005.0 28 34296.0 29 42125.0 30 52237.0 31 68725.0 32 96080.0 33 139201.0 34 212389.0 35 357771.0 36 720820.0 37 1467936.0 38 540963.0 39 2327.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 32.90099488325494 32.190769193365554 15.670550908624922 19.237685014754582 2 31.32089805468754 24.624611925104713 29.662723032276688 14.39176698793106 3 32.68173996870509 23.34615052594032 24.071057320925743 19.901052184428845 4 28.82620482148829 22.81657075550387 28.553571234194784 19.803653188813062 5 30.898238699995744 21.070718311485003 28.489437667963458 19.541605320555792 6 28.3926906822828 21.41919033793585 30.330347811935184 19.85777116784617 7 31.776076938570398 21.96589302324169 24.47520720965092 21.78282282853699 8 27.828796081875208 25.40972138414569 25.05551545438422 21.705967079594878 9 26.30307808221881 23.18922127267474 28.33304575352572 22.174654891580726 10-11 22.272650927345733 29.044948481354687 30.99186608946492 17.690534501834655 12-13 22.632102932191103 28.307592273760562 30.085856943887485 18.974447850160846 14-15 22.372514740990095 28.12688061559645 30.558876733329036 18.94172791008442 16-17 22.522624404338814 27.81569963711646 30.771707202176362 18.88996875636836 18-19 22.100180404266457 27.816739016426123 30.886394391403133 19.196686187904287 20-21 22.15108311701519 28.28927745903827 30.30734167877382 19.252297745172715 22-23 22.221979031598348 28.310441856137114 30.00578554711375 19.46179356515079 24-25 21.652778021126384 28.51292333452426 30.22166222417917 19.612636420170187 26-27 21.533854694788076 28.654912796592985 30.502940580004058 19.308291928614885 28-29 21.380518865721978 29.070686049376484 30.13398153269589 19.414813552205647 30-31 21.23223705829698 29.467183323870238 30.102946329420654 19.197633288412128 32-33 20.76838105824704 29.503998734820602 30.502469089338213 19.22515111759414 34-35 20.82272858118806 29.83171259271873 30.438362697220516 18.907196128872698 36-37 20.835014790700846 29.368565614341485 30.89332488138361 18.90309471357406 38-39 21.059682846880996 28.869908522004216 30.864008694376103 19.20639993673868 40-41 21.046119220840772 29.312602726879877 30.008097761140125 19.633180291139222 42-43 21.41632101423942 28.90360765557275 30.029600912466638 19.650470417721202 44-45 20.89526968585937 29.04802524550969 30.351132941798635 19.7055721268323 46-47 21.114194017780864 29.24318514255873 30.085912961474794 19.556707878185613 48-49 20.86064594703688 29.651603778427077 29.871486081196768 19.616264193339276 50-51 21.02342246695594 29.367627995620953 29.47766183726982 20.131287700153283 52-53 21.28141931223386 29.09366996833115 29.472390678443567 20.152520040991426 54-55 21.58748660077906 29.12754613710234 29.416217850581848 19.868749411536758 56-57 21.63647952442434 28.839175980813327 29.56221239917217 19.96213209559016 58-59 21.538753598610448 29.301183021997634 29.465346951225925 19.694716428165986 60-61 21.099620124687487 29.545843562524222 29.097995363653673 20.256540949134617 62-63 21.12960014432223 29.53847292083257 29.04763545664708 20.28429147819812 64-65 20.939977179214615 29.36795900417055 29.304984581971325 20.387079234643508 66-67 21.259222104239313 29.001019529430216 29.325287408602485 20.414470957727982 68-69 21.278175759239794 29.144613464966024 29.7292595585132 19.847951217280986 70-71 21.194027922110582 29.908681728402538 29.269480431047885 19.627809918439 72-73 20.93918835639821 30.092178851978137 29.05152016124997 19.917112630373683 74-75 21.133337497059316 29.585773029722546 28.98068567445583 20.300203798762308 76-77 21.184962197219548 29.343025061104328 29.002148232202657 20.469864509473467 78-79 20.883699646461356 29.001291975587513 29.742904712809715 20.372103665141413 80-81 20.843606591440654 29.42779747258846 29.41074365296364 20.31785228300725 82-83 20.418519191613495 29.68762000065394 29.28619207049619 20.607668737236366 84-85 20.45428682005309 29.708377570967983 29.39600009833871 20.441335510640222 86-87 20.61480265867459 29.48358604259202 29.91723639773671 19.984374900996684 88-89 20.758404572917946 29.96487663864984 29.592366507593333 19.68435228083888 90-91 21.129516251778067 29.707115992680183 29.041391039379384 20.121976716162372 92-93 20.807416749810866 29.793096354204934 29.088452423359968 20.311034472624236 94-95 20.423030953314452 29.93766773055687 29.106363896055033 20.532937420073637 96-97 20.540866669605457 30.294459214400476 28.94179218595856 20.222881930035502 98-99 20.862847564720347 30.072339404886662 28.839694596521532 20.22511843387146 100-101 20.921960472745656 30.098279600140398 28.453806940648295 20.525952986465647 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 211.0 1 223.5 2 243.5 3 244.5 4 219.5 5 235.0 6 338.5 7 484.0 8 684.0 9 933.5 10 1171.5 11 1345.0 12 1412.5 13 1325.0 14 1149.0 15 968.0 16 807.0 17 717.0 18 705.0 19 770.0 20 978.5 21 1274.0 22 1704.5 23 2470.0 24 3498.5 25 5186.5 26 8302.0 27 12822.0 28 17727.0 29 22961.5 30 28941.0 31 33780.5 32 41234.5 33 56698.5 34 78956.0 35 112106.5 36 155781.5 37 207529.5 38 259568.5 39 279387.5 40 286067.0 41 275141.5 42 241805.0 43 226834.5 44 213799.5 45 203917.0 46 208976.5 47 195261.5 48 159759.0 49 129751.0 50 103387.5 51 83824.0 52 69537.5 53 55283.0 54 44800.0 55 34721.0 56 24061.5 57 15587.5 58 10312.0 59 7317.5 60 5433.5 61 4151.0 62 3028.5 63 2086.5 64 1456.0 65 1088.5 66 830.0 67 623.5 68 498.0 69 384.5 70 276.0 71 222.0 72 171.5 73 136.0 74 110.5 75 87.5 76 81.5 77 64.5 78 45.5 79 40.0 80 37.5 81 33.0 82 34.5 83 32.5 84 23.5 85 16.0 86 14.0 87 16.0 88 15.5 89 16.5 90 13.0 91 7.5 92 8.5 93 8.0 94 4.0 95 2.5 96 2.0 97 1.5 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19293586304359422 2 0.18343188236700683 3 0.15352973039785028 4 0.14862608079344083 5 0.15097679941823505 6 0.13346015418186524 7 0.10618676282538178 8 0.09438261661270546 9 0.09380125609259506 10-11 0.07358002061049426 12-13 0.09433206352400021 14-15 0.09876809705788607 16-17 0.09078070904245625 18-19 0.08715352492785441 20-21 0.08442365813777082 22-23 0.10483446770251631 24-25 0.458213196024404 26-27 0.28908783776098346 28-29 0.12596565878131163 30-31 0.23572905263259 32-33 0.26670545773673315 34-35 0.25006085328052896 36-37 0.25295501760890465 38-39 0.27040847148439284 40-41 0.27050957766180334 42-43 0.29735326776429216 44-45 0.2735554012562948 46-47 0.29186825763977237 48-49 0.2766391396673152 50-51 0.2917292366458329 52-53 0.29092038722654884 54-55 0.2673879244342541 56-57 0.28328687083205584 58-59 0.2727465518370108 60-61 0.2479755383714373 62-63 0.24045576642653105 64-65 0.23751104900945016 66-67 0.24792498528273207 68-69 0.2528539114314941 70-71 0.2385221107835552 72-73 0.22934672518355195 74-75 0.23890125894884456 76-77 0.24021563925518113 78-79 0.24184597636592553 80-81 0.2504147249014657 82-83 0.2766770544838441 84-85 0.27020625912957186 86-87 0.2694985158876983 88-89 0.2672362651681383 90-91 0.2587812610821849 92-93 0.2345410550480166 94-95 0.23366901426785097 96-97 0.22837357822597584 98-99 0.2539660793830097 100-101 0.2616627871383843 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3956237.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.97372239029689 #Duplication Level Percentage of deduplicated Percentage of total 1 87.20648150535963 37.47587126845885 2 6.038043000062204 5.189543673306971 3 1.8606709337719816 2.3987986850283494 4 0.9979421398233098 1.7154115391338307 5 0.6136390352702722 1.318517677477714 6 0.39149690719846975 1.009444764396412 7 0.2547365262161206 0.7662883732196106 8 0.2748975654728101 0.9450697331517605 9 0.24751383889093156 0.9572931900230006 >10 1.6950804134532058 15.025041215566102 >50 0.229632436680034 7.024664668047819 >100 0.15736329305699223 13.494890604448198 >500 0.022915825839438683 6.893849835100892 >1k 0.00958657890439944 5.785314772640547 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 6.319136088156498E-4 0.0 0.0 2 6.824666975209018E-4 0.0 0.0 3 9.099555966945357E-4 0.0 0.0 4 0.0011627210402207957 0.0 0.0 5 0.0013143803063365516 0.0 0.0 6 0.0023254420804415914 0.0 0.0 7 0.0030837384110203713 0.0 0.0 8 0.004271735995593793 0.0 0.0 9 0.004853096515704191 0.0 0.0 10-11 0.0068120287030327045 0.0 0.0 12-13 0.0102117239184609 0.0 0.0 14-15 0.014660395724523077 0.0 0.0 16-17 0.021017446629208514 0.0 0.0 18-19 0.028752069201112066 0.0 0.0 20-21 0.0374345621862391 0.0 0.0 22-23 0.04928926148762068 0.0 0.0 24-25 0.06331774360332812 0.0 0.0 26-27 0.07758635289038548 0.0 0.0 28-29 0.09363695855430298 0.0 0.0 30-31 0.11389610885293272 0.0 0.0 32-33 0.13200675288158925 0.0 0.0 34-35 0.15400998474055017 0.0 0.0 36-37 0.1780606166920738 0.0 0.0 38-39 0.20495485988326786 0.0 0.0 40-41 0.23566586127170844 0.0 0.0 42-43 0.2688918788232353 0.0 0.0 44-45 0.3070973756122295 0.0 0.0 46-47 0.34901852442105974 0.0 0.0 48-49 0.3940360499130866 0.0 0.0 50-51 0.4435528002998809 0.0 0.0 52-53 0.4954076310392932 0.0 0.0 54-55 0.5512182409698914 0.0 0.0 56-57 0.6107445029203256 0.0 0.0 58-59 0.6732913119208985 0.0 0.0 60-61 0.7392378161368998 0.0 0.0 62-63 0.8085713772961529 0.0 0.0 64-65 0.881822802830063 0.0 0.0 66-67 0.9601674520510273 0.0 0.0 68-69 1.0448185990879717 0.0 0.0 70-71 1.13470199080591 0.0 0.0 72-73 1.2301714988257781 0.0 0.0 74-75 1.3332492466957868 0.0 0.0 76-77 1.4420394935894891 0.0 0.0 78-79 1.5585896395994476 0.0 0.0 80-81 1.6859455083201538 0.0 0.0 82-83 1.823399356509734 0.0 0.0 84-85 1.97029399401502 0.0 0.0 86-87 2.1290938839103926 0.0 0.0 88-89 2.299268218764447 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGTCG 55 8.2518454E-7 51.76709 1 GAGCGGC 425 0.0 47.966496 9 CGAGTCG 40 5.416059E-4 47.453167 1 CGCGACA 40 5.416059E-4 47.453167 1 CGAGGCG 160 0.0 44.487347 1 TGCCGGC 100 6.4392225E-10 42.704605 2 CGACCGC 45 9.6747803E-4 42.18059 1 CGATCGC 45 9.6747803E-4 42.18059 1 TCGCCGC 45 9.678398E-4 42.177387 2 CGACGGG 125 3.6379788E-12 41.75879 1 AGAGCGG 500 0.0 40.772034 8 CGATCGG 60 8.3427745E-5 39.544308 1 TCGGGAA 240 0.0 39.536297 3 AACCGAG 195 0.0 38.935932 1 CGTTGCG 50 0.0016241443 37.962536 1 CGCTCGG 50 0.0016241443 37.962536 1 CGACGTC 75 6.973609E-6 37.962532 1 CGAGGGG 495 0.0 36.428696 1 CGGGAGA 595 0.0 35.87941 4 AAGCGGC 265 0.0 35.780186 9 >>END_MODULE