FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n02_44065-1.342000000357db.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n02_44065-1.342000000357db.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3956237
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTCG558.2518454E-751.767091
GAGCGGC4250.047.9664969
CGAGTCG405.416059E-447.4531671
CGCGACA405.416059E-447.4531671
CGAGGCG1600.044.4873471
TGCCGGC1006.4392225E-1042.7046052
CGACCGC459.6747803E-442.180591
CGATCGC459.6747803E-442.180591
TCGCCGC459.678398E-442.1773872
CGACGGG1253.6379788E-1241.758791
AGAGCGG5000.040.7720348
CGATCGG608.3427745E-539.5443081
TCGGGAA2400.039.5362973
AACCGAG1950.038.9359321
CGTTGCG500.001624144337.9625361
CGCTCGG500.001624144337.9625361
CGACGTC756.973609E-637.9625321
CGAGGGG4950.036.4286961
CGGGAGA5950.035.879414
AAGCGGC2650.035.7801869
Produced by FastQC (version 0.11.2)