##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n02_41442-1.3420000003572e.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3140202 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.105891595508822 33.0 33.0 33.0 27.0 33.0 2 30.57407962927226 33.0 33.0 33.0 27.0 33.0 3 30.44457203708551 33.0 33.0 33.0 22.0 33.0 4 33.80819195707792 37.0 37.0 37.0 22.0 37.0 5 33.90549620693191 37.0 37.0 37.0 27.0 37.0 6 34.50917456902454 37.0 37.0 37.0 27.0 37.0 7 33.902871216565046 37.0 37.0 37.0 22.0 37.0 8 34.20442219959098 37.0 37.0 37.0 27.0 37.0 9 33.61604826695862 37.0 37.0 37.0 22.0 37.0 10-11 33.645144962012 37.0 37.0 37.0 18.5 37.0 12-13 33.635784736141176 37.0 37.0 37.0 18.5 37.0 14-15 35.215702524869414 40.0 37.0 40.0 18.5 40.0 16-17 35.12379410623903 40.0 37.0 40.0 18.5 40.0 18-19 35.15396477678824 40.0 37.0 40.0 18.5 40.0 20-21 35.063176986703404 40.0 37.0 40.0 15.0 40.0 22-23 35.92925343656236 40.0 37.0 40.0 27.0 40.0 24-25 35.228056666418276 40.0 37.0 40.0 22.0 40.0 26-27 35.097782403807145 40.0 37.0 40.0 18.5 40.0 28-29 34.84018432572172 40.0 37.0 40.0 15.0 40.0 30-31 34.76770682268211 40.0 37.0 40.0 15.0 40.0 32-33 34.55344958700109 40.0 37.0 40.0 15.0 40.0 34-35 34.81466160457194 40.0 37.0 40.0 18.5 40.0 36-37 34.55174874100456 38.5 37.0 40.0 15.0 40.0 38-39 34.47282133442371 37.0 37.0 40.0 15.0 40.0 40-41 34.304065630172836 37.0 37.0 40.0 15.0 40.0 42-43 34.20775208091709 37.0 37.0 40.0 15.0 40.0 44-45 34.03048736992079 37.0 37.0 40.0 15.0 40.0 46-47 33.97480814928467 37.0 35.0 40.0 15.0 40.0 48-49 33.86546566112626 37.0 33.0 40.0 15.0 40.0 50-51 32.889681937658786 37.0 33.0 38.5 15.0 38.5 52-53 33.29563846529618 37.0 33.0 38.5 15.0 40.0 54-55 33.94581670223762 37.0 33.0 40.0 15.0 40.0 56-57 34.2471727933426 37.0 33.0 40.0 18.5 40.0 58-59 33.66551562606482 37.0 33.0 40.0 15.0 40.0 60-61 33.43750863797934 37.0 33.0 40.0 15.0 40.0 62-63 33.050334341548734 37.0 33.0 40.0 6.0 40.0 64-65 32.84626307479583 37.0 33.0 38.5 10.5 40.0 66-67 32.73399099803134 37.0 33.0 37.0 15.0 40.0 68-69 32.83743338804319 37.0 33.0 37.0 15.0 40.0 70-71 32.371870503872046 37.0 33.0 37.0 15.0 40.0 72-73 32.653988023700386 37.0 33.0 37.0 18.5 37.0 74-75 32.76474825504856 37.0 33.0 37.0 22.0 37.0 76-77 32.4773882380815 37.0 33.0 37.0 22.0 37.0 78-79 31.999421056352425 37.0 33.0 37.0 22.0 37.0 80-81 31.498710592503286 37.0 33.0 37.0 15.0 37.0 82-83 31.136288366162432 37.0 33.0 37.0 15.0 37.0 84-85 30.90818711662498 33.0 33.0 37.0 15.0 37.0 86-87 30.754773896711104 33.0 33.0 37.0 15.0 37.0 88-89 30.6200136169584 33.0 33.0 37.0 15.0 37.0 90-91 30.463408086486155 33.0 30.0 37.0 15.0 37.0 92-93 30.32164156955508 33.0 27.0 37.0 15.0 37.0 94-95 30.21081207514676 33.0 27.0 37.0 6.0 37.0 96-97 30.084076438394728 33.0 27.0 37.0 6.0 37.0 98-99 29.95606492830716 33.0 27.0 37.0 6.0 37.0 100-101 28.773203125149273 33.0 24.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.07036841515239445 2306 2 0.029823765887549314 2306 3 0.03037511670174453 2306 4 0.20726136439036225 2306 5 0.18016861251882688 2306 6 -0.19115696860189502 2306 7 0.36249117821061105 2306 8 -0.0955738875736003 2306 9 0.29127300981534887 2306 10-11 0.22351415643541372 2306 12-13 0.14455978378897072 2306 14-15 0.46078816505983866 2306 16-17 0.5021932411989312 2306 18-19 0.5559084771342739 2306 20-21 0.6283208258878688 2306 22-23 0.23340582314536107 2306 24-25 0.5225857315339297 2306 26-27 0.2216593272525671 2306 28-29 0.35975415058754123 2306 30-31 0.3330270145119272 2306 32-33 0.31419301104680386 2306 34-35 0.1794457982275759 2306 36-37 0.42304112910351677 2306 38-39 0.09097863682966789 2306 40-41 0.33680431380140874 2306 42-43 0.24310348930035985 2306 44-45 0.29698567681844423 2306 46-47 0.27816288573735903 2306 48-49 0.27735487551231586 2306 50-51 0.1901151164571928 2306 52-53 0.12729785096788504 2306 54-55 0.41311904275972466 2306 56-57 0.2034267420118283 2306 58-59 0.5268505753342936 2306 60-61 0.4961344158507188 2306 62-63 0.5267548407070421 2306 64-65 0.4695691975804337 2306 66-67 0.34176702235854606 2306 68-69 0.05403915916112112 2306 70-71 0.20413394706011445 2306 72-73 -0.14315934170379307 2306 74-75 -0.16690707032444152 2306 76-77 0.09441695378465198 2306 78-79 0.2658318948049825 2306 80-81 0.28590628207552626 2306 82-83 0.44818937565936423 2306 84-85 0.45853155358996744 2306 86-87 0.4844308884621604 2306 88-89 0.46044628074012195 2306 90-91 0.34746089403415326 2306 92-93 0.4248608259632114 2306 94-95 0.3575193248068942 2306 96-97 0.3236807618720441 2306 98-99 0.425602413878746 2306 100-101 0.23228549780763075 2307 1 -0.1819037055628563 2307 2 -0.23385290658027813 2307 3 -0.12158688970030695 2307 4 0.017298968048130803 2307 5 -0.0963377312006628 2307 6 -0.23639881941141994 2307 7 0.24929745624755384 2307 8 -0.30761584769422967 2307 9 0.00819521515081334 2307 10-11 -0.03622116367511552 2307 12-13 -0.035788549456462704 2307 14-15 0.2644556803258382 2307 16-17 0.11128200373015318 2307 18-19 0.2810821523364737 2307 20-21 0.28398966119726765 2307 22-23 0.10780952629860252 2307 24-25 0.12937880114623823 2307 26-27 -0.14270641266691797 2307 28-29 0.08446816781842159 2307 30-31 0.16410660545828648 2307 32-33 0.11272358379541458 2307 34-35 -0.005800559267669314 2307 36-37 0.23105232230448536 2307 38-39 -0.02985767299379205 2307 40-41 -0.04537062551015936 2307 42-43 0.1163028126582546 2307 44-45 0.12096524011739263 2307 46-47 0.22869741541995836 2307 48-49 0.06985655752887965 2307 50-51 -0.07059964869174706 2307 52-53 -0.10460269191194982 2307 54-55 0.05107676301599895 2307 56-57 -0.04696379746402357 2307 58-59 0.2875515567370286 2307 60-61 0.11048437437962377 2307 62-63 0.19575535530022847 2307 64-65 0.16596660575670796 2307 66-67 0.06367614711862757 2307 68-69 0.006546562431189784 2307 70-71 -0.03657780564110169 2307 72-73 -0.2743300651866676 2307 74-75 -0.09703246645732122 2307 76-77 0.06137212025235783 2307 78-79 0.16919944379121432 2307 80-81 0.3521913187602692 2307 82-83 0.2927049229260241 2307 84-85 0.19472703188196405 2307 86-87 0.13340961275047647 2307 88-89 0.24769855286083597 2307 90-91 0.22672726313171054 2307 92-93 0.21727481139159366 2307 94-95 0.09668030617766021 2307 96-97 0.2706922459300394 2307 98-99 0.19346994448665455 2307 100-101 0.10204407848929264 2201 1 0.008622825551650948 2201 2 -0.02356527775219064 2201 3 -0.03090622034561008 2201 4 0.0015974832390170945 2201 5 -0.06588524077736935 2201 6 -0.1335896519816515 2201 7 0.3355645301140484 2201 8 -0.19817370187258376 2201 9 -0.06666570884760858 2201 10-11 -0.017897171326886507 2201 12-13 -0.03162406022217823 2201 14-15 0.24583923284071574 2201 16-17 0.23130187629642052 2201 18-19 0.17452500452517228 2201 20-21 0.2643468240308522 2201 22-23 0.18117741088909156 2201 24-25 0.3568475699740432 2201 26-27 0.09677539039090988 2201 28-29 0.1805526649793734 2201 30-31 0.3548468845212156 2201 32-33 0.2426981178248866 2201 34-35 -0.011826253768703054 2201 36-37 0.22782293040341983 2201 38-39 0.13299349291137474 2201 40-41 0.15017478733901157 2201 42-43 0.18925019310723457 2201 44-45 0.2570599572269785 2201 46-47 0.25077198508741105 2201 48-49 0.24717845954202744 2201 50-51 0.17456265591865616 2201 52-53 0.24614650463548315 2201 54-55 0.3485879378386443 2201 56-57 0.404912350182677 2201 58-59 0.48622847691275695 2201 60-61 0.3424668206789505 2201 62-63 0.45015316939785066 2201 64-65 0.4695691975804337 2201 66-67 0.3178580158590094 2201 68-69 0.1784588434693859 2201 70-71 0.356176658295027 2201 72-73 0.2486698133565568 2201 74-75 0.18128234471734572 2201 76-77 0.30379485536311535 2201 78-79 0.5272060823630085 2201 80-81 0.49853395152770474 2201 82-83 0.41104917138823893 2201 84-85 0.4148918135713977 2201 86-87 0.3867057259737656 2201 88-89 0.43351963264355575 2201 90-91 0.391100634052723 2201 92-93 0.5260678826020246 2201 94-95 0.4127653786601897 2201 96-97 0.305807038566563 2201 98-99 0.42374540366518687 2201 100-101 0.041941950918126025 1101 1 -0.11026911415829943 1101 2 -0.12917542181671848 1101 3 -0.13161626742600419 1101 4 0.012537737971527463 1101 5 -0.05145519511644636 1101 6 -0.1337397882546938 1101 7 0.2149947688073226 1101 8 -0.2610809952587445 1101 9 0.013181440727507265 1101 10-11 0.03257523148132435 1101 12-13 -0.01759996744497272 1101 14-15 0.13419575540386575 1101 16-17 0.13979869243252807 1101 18-19 0.2028979328883338 1101 20-21 0.1651239059822558 1101 22-23 -0.021558910429050115 1101 24-25 0.08865651318236445 1101 26-27 -0.12937809489593377 1101 28-29 0.02904147514745148 1101 30-31 0.10764068658389192 1101 32-33 0.02821012346220897 1101 34-35 -0.13709654780572578 1101 36-37 0.08785000562793499 1101 38-39 0.0644636935909304 1101 40-41 -0.03158174466126695 1101 42-43 0.023415905336158005 1101 44-45 0.10320469171390556 1101 46-47 0.1170073901843196 1101 48-49 0.1738817360614533 1101 50-51 0.08837449110257012 1101 52-53 0.06952141596733696 1101 54-55 0.12949085738470956 1101 56-57 0.1323591522215608 1101 58-59 0.27586552539740694 1101 60-61 0.1376730474736263 1101 62-63 0.22875568926945533 1101 64-65 0.18024207325940722 1101 66-67 0.11010636309261912 1101 68-69 0.13399042921651017 1101 70-71 0.3562005436111946 1101 72-73 -0.08217496250558298 1101 74-75 0.057077433272397116 1101 76-77 0.15898266804139638 1101 78-79 0.3597500303698453 1101 80-81 0.3139482282003918 1101 82-83 0.25603454187581676 1101 84-85 0.30477621644044106 1101 86-87 0.36362753851244634 1101 88-89 0.2900886990338165 1101 90-91 0.337769500118295 1101 92-93 0.20148807893347254 1101 94-95 0.1368974837618211 1101 96-97 0.12102496819125719 1101 98-99 0.164946930609581 1101 100-101 0.05626924097399666 2304 1 -0.043698597118584814 2304 2 -0.15353573828432943 2304 3 -0.15207080667046569 2304 4 -0.06651438936710008 2304 5 -0.1043403831072851 2304 6 -0.14526472423128922 2304 7 0.19373191048240557 2304 8 -0.246557772519985 2304 9 -0.041240019637776015 2304 10-11 -0.0214675452980444 2304 12-13 -0.041873328131607934 2304 14-15 0.17684362804916276 2304 16-17 0.09534676861221669 2304 18-19 0.12330984611964624 2304 20-21 0.13968355371007846 2304 22-23 0.09180932737267256 2304 24-25 0.03640324137064255 2304 26-27 0.05242008314739621 2304 28-29 0.053223540300635364 2304 30-31 0.24977336631835811 2304 32-33 0.05570454487114063 2304 34-35 -0.2009345477391804 2304 36-37 0.16514164004782828 2304 38-39 0.11326195987589216 2304 40-41 0.08128525173015078 2304 42-43 0.10644107606122333 2304 44-45 0.124868173484451 2304 46-47 0.07535541036513393 2304 48-49 0.1362295614355986 2304 50-51 0.1925517760987745 2304 52-53 0.18838490551729592 2304 54-55 0.3675036975871535 2304 56-57 0.3327988977320473 2304 58-59 0.42007541961514505 2304 60-61 0.2946698616131229 2304 62-63 0.319998020565194 2304 64-65 0.22821996559593316 2304 66-67 0.1095570629420024 2304 68-69 0.08298805087142114 2304 70-71 0.3126229995478411 2304 72-73 0.13727691711353884 2304 74-75 0.3210183657051857 2304 76-77 0.3676087940263457 2304 78-79 0.4529733783024241 2304 80-81 0.5549936713558239 2304 82-83 0.2499751223064557 2304 84-85 0.20578734661967601 2304 86-87 0.26771129380634306 2304 88-89 0.4129723263210856 2304 90-91 0.1201055985168793 2304 92-93 0.36215101952081596 2304 94-95 0.20891133625170255 2304 96-97 0.3634846884572447 2304 98-99 0.3603384188762604 2304 100-101 0.3126571556956108 2202 1 -0.15832965131889054 2202 2 -0.22209297714639575 2202 3 -0.19603747628738688 2202 4 -0.12623125646400268 2202 5 -0.21805976747668154 2202 6 -0.2690465500890582 2202 7 0.10057662285971247 2202 8 -0.288046644870839 2202 9 -0.2394513350643166 2202 10-11 -0.02195018181063091 2202 12-13 -0.04726044915390304 2202 14-15 0.13383641405648916 2202 16-17 0.06458933885629392 2202 18-19 0.12623038914909301 2202 20-21 0.10162050721200444 2202 22-23 0.045755041880397584 2202 24-25 -0.01111391269034101 2202 26-27 -0.1236828233507623 2202 28-29 -0.038648387124787575 2202 30-31 -0.01644980663338913 2202 32-33 -0.04260246680063773 2202 34-35 -0.27213205556462583 2202 36-37 -0.00447548771018802 2202 38-39 -0.22409888477748297 2202 40-41 -0.174479896470217 2202 42-43 -0.16351873816423534 2202 44-45 -0.06837175732172085 2202 46-47 -0.03448272876465808 2202 48-49 -0.08525862944828333 2202 50-51 -0.07968492729646925 2202 52-53 -0.11368072889312941 2202 54-55 0.09467632023354611 2202 56-57 0.11213054485016727 2202 58-59 0.09402983529253106 2202 60-61 0.047186822453284094 2202 62-63 0.16998179417189618 2202 64-65 0.07371727650235727 2202 66-67 0.09071325475500913 2202 68-69 -0.04146984007974197 2202 70-71 0.04876106807931535 2202 72-73 -0.0963081434684554 2202 74-75 0.039389676278347 2202 76-77 0.1492489104907122 2202 78-79 0.2214514174061648 2202 80-81 0.19644315159182923 2202 82-83 0.0420331650086716 2202 84-85 0.08265332990794505 2202 86-87 0.072570319483102 2202 88-89 0.12684200739870377 2202 90-91 0.13611508586673438 2202 92-93 0.11264518695309178 2202 94-95 0.027748537533362594 2202 96-97 0.12069275985171046 2202 98-99 0.19529584588968874 2202 100-101 0.11159344393619008 1102 1 0.022329165364464387 1102 2 0.1177022460973518 1102 3 0.19078447996910342 1102 4 0.22598376267236375 1102 5 0.20228054015872488 1102 6 0.06487541595151924 1102 7 0.43563578966898575 1102 8 0.07322664865925077 1102 9 0.3139624720187939 1102 10-11 0.3311216466481923 1102 12-13 0.3201255122675022 1102 14-15 0.3175453351216788 1102 16-17 0.2467834376515583 1102 18-19 0.327268246697038 1102 20-21 0.4432410537304179 1102 22-23 0.04644585164918169 1102 24-25 0.11301149013952028 1102 26-27 0.03740081049794952 1102 28-29 0.10402847355125999 1102 30-31 0.2713705776964943 1102 32-33 0.2632244106891264 1102 34-35 0.18751960921301958 1102 36-37 0.3502507785372089 1102 38-39 0.19157267014241341 1102 40-41 0.19493653631297292 1102 42-43 0.16248868918591342 1102 44-45 0.2575654958241884 1102 46-47 0.2883288101953383 1102 48-49 0.26376886845717706 1102 50-51 0.19864581588194596 1102 52-53 0.1805379671516718 1102 54-55 0.3906497989038229 1102 56-57 0.5165151073069794 1102 58-59 0.5090001978372669 1102 60-61 0.5245529237809308 1102 62-63 0.5689052278244162 1102 64-65 0.4793464691667637 1102 66-67 0.5665310651757025 1102 68-69 0.3803060766463844 1102 70-71 0.45686266469209613 1102 72-73 0.28641982894654205 1102 74-75 0.3666387412025003 1102 76-77 0.3333756398512193 1102 78-79 0.28148431927501605 1102 80-81 0.3813829611797104 1102 82-83 0.3921952374941675 1102 84-85 0.5322543263450008 1102 86-87 0.5224394314612226 1102 88-89 0.5093783901890312 1102 90-91 0.6074651996906368 1102 92-93 0.47294050003692334 1102 94-95 0.7039198085195082 1102 96-97 0.4803888743994662 1102 98-99 0.3541973001752581 1102 100-101 0.3828255380627006 2305 1 0.03166187511509122 2305 2 -0.061213358703877674 2305 3 0.07082937703400205 2305 4 0.09352729523280345 2305 5 0.08373128018471476 2305 6 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1213 64-65 -0.6263468506127978 1213 66-67 -0.5204825276980571 1213 68-69 -0.3504452502205879 1213 70-71 -1.4272856959105908 1213 72-73 -0.6971606978936187 1213 74-75 -0.7897484748156032 1213 76-77 -0.851971813211442 1213 78-79 -0.8011602852423358 1213 80-81 -0.8620126104595442 1213 82-83 -0.9566641900228348 1213 84-85 -0.9190164695209084 1213 86-87 -0.8540443002596838 1213 88-89 -0.9274439815625719 1213 90-91 -0.8192619161883066 1213 92-93 -0.9006585168332428 1213 94-95 -1.0500780211630136 1213 96-97 -0.9444118716164631 1213 98-99 -0.9043333896915442 1213 100-101 -0.765959245963117 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 336.0 3 202.0 4 334.0 5 227.0 6 684.0 7 3490.0 8 17990.0 9 63657.0 10 85959.0 11 56109.0 12 30012.0 13 19488.0 14 14637.0 15 12851.0 16 12038.0 17 11216.0 18 10719.0 19 10942.0 20 11420.0 21 12104.0 22 12945.0 23 13731.0 24 15009.0 25 17019.0 26 19736.0 27 24056.0 28 29940.0 29 37036.0 30 42947.0 31 54712.0 32 75035.0 33 107777.0 34 162119.0 35 273375.0 36 549562.0 37 1027415.0 38 302953.0 39 420.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.13441533477012 23.00292089340095 23.615782076785518 13.246881695043413 2 30.88416241870276 25.61149784712051 31.822547987184493 11.681791746992237 3 36.39419896780103 19.70465408066869 29.29791078897329 14.603236162556984 4 27.462415540405864 17.499858881878872 35.88227208785615 19.15545348985911 5 33.61851492531125 16.095984558989088 33.36717114020971 16.918329375489947 6 30.22968615330458 16.528838393839067 38.258992158854696 14.982483294001655 7 36.04172245387195 18.919768435280464 28.65667597516035 16.381833135687234 8 27.574196785196435 32.49116517105956 24.15166676813399 15.782971275610008 9 32.49049673258446 27.310251799134427 23.18092831447767 17.01832315380344 10-11 26.99778548772649 29.742279720720973 26.880112634511143 16.379822157041392 12-13 29.148648489278024 23.855710934833034 26.9271335184795 20.068507057409445 14-15 25.43864122902421 24.40331915078089 29.32645413663446 20.831585483560435 16-17 26.721313173569193 25.722743480945837 30.15156477220662 17.404378573278354 18-19 26.436754499516578 25.867724355914834 30.507869004661785 17.187652139906803 20-21 25.099681716490394 28.455179999180906 29.223582970676897 17.221555313651802 22-23 28.519140190854408 25.76585548255761 28.44950356208331 17.26550076450467 24-25 24.799328282121508 29.645181023969936 28.229615561464183 17.325875132444374 26-27 22.396705313005324 31.869872510035226 28.189520507884076 17.543901669075375 28-29 23.698101741454618 30.119665820792683 27.95074673168386 18.231485706068835 30-31 26.494854903162384 26.91433030755678 28.334354692507052 18.256460096773782 32-33 22.70140887879709 29.281455211395674 30.352673064004883 17.664462845802355 34-35 27.131446652004925 27.114432267827475 28.565139952886437 17.18898112728116 36-37 24.081886862080477 27.45515976359317 30.00422018243191 18.458733191894442 38-39 20.653339823330114 29.93916506053082 31.493747045617344 17.913748070521724 40-41 21.786869462338913 31.09843345483641 28.846372125077764 18.26832495774691 42-43 24.50256031433733 27.519878025976645 30.298466206677176 17.67909545300885 44-45 22.0677093610687 29.272633167363054 31.050156359458803 17.609501112109445 46-47 23.74311105064601 30.091946336373944 28.44637485656838 17.71856775641167 48-49 20.795228310830336 29.784118104415825 30.125295157265537 19.295358427488303 50-51 19.81588645202827 27.468596795257987 31.880286594012734 20.835230158701012 52-53 25.25321798774232 26.937974067680532 27.982525711407895 19.826282233169245 54-55 27.16324115536755 26.96650486421061 27.54046271979781 18.329791260624024 56-57 27.697942520211168 26.898085577045126 27.004771488262076 18.39920041448163 58-59 25.911794357655033 26.706732323470177 27.60312489772721 19.77834842114758 60-61 21.642381230932028 27.01315076495332 27.429340275145382 23.91512772896927 62-63 24.42886024846901 27.034985601411577 27.60553485441498 20.930619295704425 64-65 21.78533907500523 28.55980120022285 29.8878567405729 19.767002984199017 66-67 20.28919767810626 28.386601787626432 28.74913233833482 22.575068195932488 68-69 20.037480652739884 27.24601797992338 33.70427187184113 19.012229495495603 70-71 19.41513173245017 31.813730637837022 30.430861489974877 18.34027613973793 72-73 19.20551296777453 35.57997132066944 26.743156583846826 18.471359127709196 74-75 19.960666608893852 35.169966180561104 26.257536200173544 18.611831010371496 76-77 20.204603049942314 34.563650904125495 26.55902458533817 18.67272146059402 78-79 20.050589226113157 34.34974618406288 27.112014007556308 18.487650582267662 80-81 19.226985872807546 34.60490993797098 27.08459125534911 19.083512933872367 82-83 18.906747462904264 33.76847841947948 27.315044978509363 20.009729139106895 84-85 19.001874343177693 32.77674020096548 27.912435718617683 20.30894973723915 86-87 19.216316824450335 32.57889209908842 28.504124001326936 19.700667075134316 88-89 19.647893990494865 32.90607425409785 27.862551489074487 19.58348026633279 90-91 20.090059747187826 32.100090506693824 27.556095793434032 20.253753952684313 92-93 19.623277626046765 32.67059844505591 27.592421953571268 20.11370197532606 94-95 19.38136521784369 33.00245664015759 27.661235163052027 19.954942978946686 96-97 19.963035670801084 33.254587588576996 26.992992352130713 19.789384388491207 98-99 20.597775226388777 32.18427718321333 26.610786884654537 20.607160705743354 100-101 20.515083131657168 32.20208981255091 26.1864121479154 21.09641490787652 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 528.0 1 423.0 2 321.5 3 400.5 4 430.0 5 391.5 6 463.0 7 638.5 8 857.5 9 1035.0 10 1185.0 11 1351.5 12 1389.0 13 1279.5 14 1161.5 15 1108.0 16 1077.0 17 1051.5 18 1065.0 19 1097.5 20 1157.5 21 1298.0 22 1516.5 23 1804.0 24 2327.0 25 3155.5 26 4664.0 27 7223.5 28 9813.5 29 11827.5 30 14178.0 31 16729.5 32 20229.5 33 29387.0 34 46788.0 35 77322.5 36 116210.0 37 157526.5 38 203573.0 39 234168.5 40 239905.0 41 219589.5 42 192807.5 43 186996.5 44 180453.0 45 167611.0 46 164685.5 47 151933.0 48 123358.5 49 100881.5 50 85022.5 51 73407.0 52 63327.5 53 51471.0 54 40644.5 55 31124.0 56 23094.0 57 16813.0 58 12373.5 59 9245.5 60 6940.5 61 5189.0 62 3786.0 63 2805.0 64 2112.0 65 1566.0 66 1162.5 67 879.0 68 684.0 69 514.0 70 379.5 71 282.0 72 218.5 73 178.0 74 139.5 75 113.0 76 100.0 77 82.0 78 60.5 79 55.5 80 49.0 81 37.0 82 34.0 83 31.0 84 28.0 85 23.0 86 18.5 87 18.0 88 16.0 89 14.5 90 12.0 91 10.0 92 10.5 93 7.5 94 4.5 95 2.5 96 2.5 97 3.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18747201613144632 2 0.17715420855091488 3 0.15158260519546196 4 0.1442900807018147 5 0.14827071634245184 6 0.12906813001201833 7 0.10451556938056851 8 0.09244628211815674 9 0.09107694345777756 10-11 0.07212911780834481 12-13 0.09110878854290265 14-15 0.0959810865670425 16-17 0.0880357378283308 18-19 0.08504229982657167 20-21 0.08249469301656391 22-23 0.1020157301982484 24-25 0.4423123098450355 26-27 0.2820678414955471 28-29 0.12104316856049388 30-31 0.22680069626094118 32-33 0.2568624566190328 34-35 0.24070107591804604 36-37 0.24316907001524107 38-39 0.26120931073860854 40-41 0.2605724090361066 42-43 0.28799102732881515 44-45 0.26482372790030706 46-47 0.2840263142307406 48-49 0.2674031797954399 50-51 0.28257736285754864 52-53 0.2820996865806722 54-55 0.25764266120459767 56-57 0.2732149078307701 58-59 0.2630244805907391 60-61 0.23866299047003983 62-63 0.2310679376677042 64-65 0.2293005354432613 66-67 0.2388540609807904 68-69 0.24369451391980518 70-71 0.23001704985857596 72-73 0.22084566534254801 74-75 0.23057433884826517 76-77 0.2311157052953918 78-79 0.23315379074339804 80-81 0.24060554066267073 82-83 0.26663889775243754 84-85 0.26022211309973053 86-87 0.260206190557168 88-89 0.2581203374814741 90-91 0.24956993212538556 92-93 0.22606825930306393 94-95 0.22485814606831028 96-97 0.2200813832995457 98-99 0.24531861326118512 100-101 0.2519423909672053 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3140202.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.44969379953641 #Duplication Level Percentage of deduplicated Percentage of total 1 89.07093097743744 42.26388401319049 2 4.549692312649423 4.317630142346395 3 1.956013409082209 2.784367119862145 4 0.9333481675546259 1.7714833903530163 5 0.5816003236837197 1.3798378636251882 6 0.42759336622481375 1.2173504578848269 7 0.28107028670940337 0.9335689328356369 8 0.24050087774928058 0.9129354406178484 9 0.22440860280575872 0.9583307540203536 >10 1.4193876740775895 13.089558999299033 >50 0.15799799355503746 5.236949062992659 >100 0.13254821264919012 13.798305498863037 >500 0.019689787646696648 6.401692535641958 >1k 0.0048820083010892015 3.7170421419790496 >5k 2.687999064465296E-4 0.7106473567179983 >10k+ 6.719996721812923E-5 0.5064162897702085 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT 15882 0.5057636419567914 Illumina Single End PCR Primer 1 (97% over 34bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 5876 0.18712172019507026 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 5790 0.1843830428743119 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 5616 0.17884199806254503 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 5005 0.15938465105111072 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4547 0.14479960206381629 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 3810 0.12132977432661976 No Hit TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA 3462 0.11024768470308598 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3422 0.1089738812980821 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.004044325810887325 0.0 0.0 2 0.00452200208776378 0.0 0.0 3 0.005063368534890431 0.0 0.0 4 0.0054773546415166924 0.0 0.0 5 0.006464552280394701 0.0 0.0 6 0.007642820430023291 0.0 0.0 7 0.009203229601153046 0.0 0.0 8 0.010859174027658094 0.0 0.0 9 0.012419583198787849 0.0 0.0 10-11 0.020428622107749757 0.0 0.0 12-13 0.028087365080335595 0.0 0.0 14-15 0.037179136883550803 0.0 0.0 16-17 0.04571361969707681 0.0 3.1845085125097046E-5 18-19 0.0555219059156067 0.0 3.1845085125097046E-5 20-21 0.06364240262250645 0.0 3.1845085125097046E-5 22-23 0.07303670273441007 0.0 3.1845085125097046E-5 24-25 0.08501045474144657 0.0 3.1845085125097046E-5 26-27 0.09700012929104561 0.0 3.1845085125097046E-5 28-29 0.10994515639439756 0.0 3.1845085125097046E-5 30-31 0.20762995501563275 0.0 3.1845085125097046E-5 32-33 0.22622748472868942 0.0 3.1845085125097046E-5 34-35 0.2394750401407298 0.0 3.1845085125097046E-5 36-37 0.2533594972552721 0.0 3.1845085125097046E-5 38-39 0.2688362086260693 0.0 3.1845085125097046E-5 40-41 0.284503990507617 0.0 3.1845085125097046E-5 42-43 0.3015251885069814 0.0 3.1845085125097046E-5 44-45 0.3210143806035408 0.0 3.1845085125097046E-5 46-47 0.34051949524266273 0.0 3.1845085125097046E-5 48-49 0.36110734277603795 0.0 3.1845085125097046E-5 50-51 0.38392434626816996 0.0 3.1845085125097046E-5 52-53 0.41010100624099977 0.0 3.1845085125097046E-5 54-55 0.4366279621502056 0.0 3.1845085125097046E-5 56-57 0.4650656231669173 0.0 3.1845085125097046E-5 58-59 0.4966081799833259 0.0 3.1845085125097046E-5 60-61 0.5326408938023732 0.0 3.1845085125097046E-5 62-63 0.5697404179731113 0.0 3.1845085125097046E-5 64-65 0.6095786194646077 0.0 3.1845085125097046E-5 66-67 0.6511523780954218 0.0 3.1845085125097046E-5 68-69 0.6977735827185639 0.0 3.1845085125097046E-5 70-71 0.7486620287484691 0.0 3.1845085125097046E-5 72-73 0.8016204053115055 0.0 3.1845085125097046E-5 74-75 0.860072059058621 0.0 3.1845085125097046E-5 76-77 0.9224088131909985 0.0 3.1845085125097046E-5 78-79 0.9947926916803441 0.0 3.1845085125097046E-5 80-81 1.0717622624277037 0.0 3.1845085125097046E-5 82-83 1.1560880478389608 0.0 3.1845085125097046E-5 84-85 1.2457478850086714 0.0 3.1845085125097046E-5 86-87 1.3433371483745313 0.0 3.1845085125097046E-5 88-89 1.4488080703088526 0.0 3.1845085125097046E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 2900 0.0 86.65261 9 CGGGAGA 3915 0.0 86.17356 4 ATTCGGA 715 0.0 85.62956 2 CGGAAAG 2000 0.0 84.4583 5 AGAGCGG 3095 0.0 83.95593 8 GAGAGCG 3660 0.0 81.749794 7 AAGCGGC 1010 0.0 81.213554 9 TCGGAGA 1040 0.0 80.75624 4 TCGGGAG 4345 0.0 79.06891 3 GTTCGGA 795 0.0 78.20676 2 AAGGGGC 2785 0.0 75.249016 9 TTCGGAG 740 0.0 75.02588 3 AAGCGTC 420 0.0 74.50723 9 ATCCGGA 770 0.0 72.73298 2 ATCGGGA 5470 0.0 72.18984 2 GGTCCGG 410 0.0 71.77426 1 GGATCGG 1800 0.0 71.195435 1 GATCGGG 5700 0.0 71.02889 1 TCGGGAA 1270 0.0 69.87065 3 AAAGCGG 1335 0.0 68.90519 8 >>END_MODULE