FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n02_41442-1.3420000003572e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n02_41442-1.3420000003572e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3140202
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT158820.5057636419567914Illumina Single End PCR Primer 1 (97% over 34bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC58760.18712172019507026No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG57900.1843830428743119No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC56160.17884199806254503No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG50050.15938465105111072No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45470.14479960206381629No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA38100.12132977432661976No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA34620.11024768470308598No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34220.1089738812980821No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC29000.086.652619
CGGGAGA39150.086.173564
ATTCGGA7150.085.629562
CGGAAAG20000.084.45835
AGAGCGG30950.083.955938
GAGAGCG36600.081.7497947
AAGCGGC10100.081.2135549
TCGGAGA10400.080.756244
TCGGGAG43450.079.068913
GTTCGGA7950.078.206762
AAGGGGC27850.075.2490169
TTCGGAG7400.075.025883
AAGCGTC4200.074.507239
ATCCGGA7700.072.732982
ATCGGGA54700.072.189842
GGTCCGG4100.071.774261
GGATCGG18000.071.1954351
GATCGGG57000.071.028891
TCGGGAA12700.069.870653
AAAGCGG13350.068.905198
Produced by FastQC (version 0.11.2)