##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n02_40825-1.342000000356f6.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1864159 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.602220089595363 33.0 33.0 33.0 27.0 33.0 2 31.344951262204564 33.0 33.0 33.0 27.0 33.0 3 31.287424516900114 33.0 33.0 33.0 27.0 33.0 4 34.90842465690963 37.0 37.0 37.0 33.0 37.0 5 34.94770188594428 37.0 37.0 37.0 33.0 37.0 6 35.2015740073674 37.0 37.0 37.0 33.0 37.0 7 34.920592073959355 37.0 37.0 37.0 33.0 37.0 8 34.95310485854479 37.0 37.0 37.0 33.0 37.0 9 34.73778899761233 37.0 37.0 37.0 33.0 37.0 10-11 34.68769589933048 37.0 37.0 37.0 33.0 37.0 12-13 34.67026605563152 37.0 37.0 37.0 33.0 37.0 14-15 36.311809239447925 40.0 37.0 40.0 33.0 40.0 16-17 36.18726567851777 40.0 37.0 40.0 30.0 40.0 18-19 36.102462289965615 40.0 37.0 40.0 27.0 40.0 20-21 35.9709976455871 40.0 37.0 40.0 27.0 40.0 22-23 36.3296835194852 40.0 37.0 40.0 27.0 40.0 24-25 35.87465312776432 40.0 37.0 40.0 27.0 40.0 26-27 35.72456104870883 38.5 37.0 40.0 27.0 40.0 28-29 35.58555117884258 37.0 37.0 40.0 27.0 40.0 30-31 35.502084586132405 37.0 37.0 40.0 27.0 40.0 32-33 35.33223936370234 37.0 37.0 40.0 27.0 40.0 34-35 35.24627781213941 37.0 37.0 40.0 27.0 40.0 36-37 35.07533477562804 37.0 37.0 40.0 24.5 40.0 38-39 34.96080028581253 37.0 37.0 40.0 22.0 40.0 40-41 34.86505952550185 37.0 37.0 40.0 22.0 40.0 42-43 34.62464521534912 37.0 35.0 40.0 22.0 40.0 44-45 34.38384494026529 37.0 33.0 40.0 22.0 40.0 46-47 34.230772965181615 37.0 33.0 40.0 22.0 40.0 48-49 34.03715053276035 37.0 33.0 40.0 22.0 40.0 50-51 32.806975692524084 35.0 33.0 38.5 18.5 38.5 52-53 33.25371145916201 37.0 33.0 37.0 22.0 40.0 54-55 33.913533126734364 37.0 33.0 40.0 22.0 40.0 56-57 33.94493629567006 37.0 33.0 40.0 22.0 40.0 58-59 33.608046845789445 37.0 33.0 38.5 22.0 40.0 60-61 33.328933851672524 37.0 33.0 37.0 22.0 40.0 62-63 33.05380200937796 37.0 33.0 37.0 18.5 40.0 64-65 32.80459714005082 37.0 33.0 37.0 15.0 40.0 66-67 32.62038243518927 37.0 33.0 37.0 15.0 38.5 68-69 32.51775572791806 37.0 33.0 37.0 15.0 37.0 70-71 32.182615860556965 37.0 33.0 37.0 15.0 37.0 72-73 32.121323610271446 37.0 33.0 37.0 15.0 37.0 74-75 32.053234729441 37.0 33.0 37.0 22.0 37.0 76-77 31.84681403249401 35.0 33.0 37.0 22.0 37.0 78-79 31.626897169179237 33.0 33.0 37.0 18.5 37.0 80-81 31.374059830733323 33.0 33.0 37.0 15.0 37.0 82-83 31.102601763047037 33.0 33.0 37.0 15.0 37.0 84-85 30.947922092482457 33.0 33.0 37.0 15.0 37.0 86-87 30.8100679716698 33.0 33.0 37.0 15.0 37.0 88-89 30.65111801085637 33.0 27.0 37.0 15.0 37.0 90-91 30.490455213316032 33.0 27.0 37.0 15.0 37.0 92-93 30.33857975634053 33.0 27.0 37.0 15.0 37.0 94-95 30.189220983832385 33.0 27.0 37.0 15.0 37.0 96-97 30.027795912258554 33.0 27.0 37.0 15.0 37.0 98-99 29.862944899013442 33.0 27.0 37.0 15.0 37.0 100-101 28.475804102547045 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.10911905503739305 2306 2 0.047032484130202334 2306 3 0.025507643540223057 2306 4 0.17467377644800308 2306 5 0.09926143271704291 2306 6 -0.12671025362598698 2306 7 0.26952009732243454 2306 8 0.022833278432159432 2306 9 0.15328489701652614 2306 10-11 0.20080817966086784 2306 12-13 0.13134087342073286 2306 14-15 0.4158954773490251 2306 16-17 0.4264592682415369 2306 18-19 0.5005976413047932 2306 20-21 0.5761513783293921 2306 22-23 0.21317399691239558 2306 24-25 0.4683672143433313 2306 26-27 0.3619413795735298 2306 28-29 0.30847745340792443 2306 30-31 0.47334443052088915 2306 32-33 0.3557973110952588 2306 34-35 0.3634642944951807 2306 36-37 0.3949342823788413 2306 38-39 0.27585132013832236 2306 40-41 0.09734947787203652 2306 42-43 0.11129211572919218 2306 44-45 0.38753692766565706 2306 46-47 0.3165653292435451 2306 48-49 0.3007449583469537 2306 50-51 0.08465635873027821 2306 52-53 0.2977534173151781 2306 54-55 0.5595725267494629 2306 56-57 0.46550510777380083 2306 58-59 0.4434075592502751 2306 60-61 0.53280957173898 2306 62-63 0.4591388565933059 2306 64-65 0.4233417039418583 2306 66-67 0.4234139872810303 2306 68-69 0.26772439714554963 2306 70-71 0.29691657927882176 2306 72-73 0.2444743105180649 2306 74-75 0.2930067530209257 2306 76-77 0.2472376996000989 2306 78-79 0.4096953942027106 2306 80-81 0.39866516109822214 2306 82-83 0.39654804809955024 2306 84-85 0.40387120433727475 2306 86-87 0.5050500637452942 2306 88-89 0.5284470037120563 2306 90-91 0.5614900228914941 2306 92-93 0.39501726179266683 2306 94-95 0.4229958706803849 2306 96-97 0.5006995358412141 2306 98-99 0.3491009149238202 2306 100-101 0.0036532770919812663 2307 1 0.1378342747797987 2307 2 0.08806590872350384 2307 3 0.044750229928371965 2307 4 0.13939105332116242 2307 5 0.13481405971839422 2307 6 -0.05344104176420217 2307 7 0.29305089377993454 2307 8 -0.01427590453903349 2307 9 0.1609603770767265 2307 10-11 0.19476370366199092 2307 12-13 0.2793261898936592 2307 14-15 0.4743041411215927 2307 16-17 0.5290820626872801 2307 18-19 0.43530879216356766 2307 20-21 0.561158005391249 2307 22-23 0.3620188934130084 2307 24-25 0.5907589313668424 2307 26-27 0.40419835264175674 2307 28-29 0.5176808446484742 2307 30-31 0.5261148921652321 2307 32-33 0.35867478891682225 2307 34-35 0.32394015724219827 2307 36-37 0.3577364707273105 2307 38-39 0.2607830264223949 2307 40-41 0.22125331835364648 2307 42-43 0.31291834565892884 2307 44-45 0.3294548901230243 2307 46-47 0.39047384931947704 2307 48-49 0.34553529361388513 2307 50-51 0.197363915282466 2307 52-53 0.25113544048814873 2307 54-55 0.32421634817236367 2307 56-57 0.410907004569367 2307 58-59 0.34732627044272846 2307 60-61 0.4817327450900635 2307 62-63 0.35831014298069164 2307 64-65 0.36432800171154156 2307 66-67 0.17212355369535004 2307 68-69 0.26835212454547275 2307 70-71 0.112356924377643 2307 72-73 0.10928059422059277 2307 74-75 0.19736860761506136 2307 76-77 0.2451711151178202 2307 78-79 0.3455927513190993 2307 80-81 0.3182730394552422 2307 82-83 0.3029592341261882 2307 84-85 0.19281866431019878 2307 86-87 0.12200966986525685 2307 88-89 0.4227888542221798 2307 90-91 0.35446855158922475 2307 92-93 0.3339232671775072 2307 94-95 0.20760927001332874 2307 96-97 0.09694862828352768 2307 98-99 0.18259953089834724 2307 100-101 0.04063270767516869 2201 1 0.11780206943467064 2201 2 0.04648829051398806 2201 3 0.07072249117990381 2201 4 0.13423764347394496 2201 5 0.1769268930492629 2201 6 0.08222628552492495 2201 7 0.17521944864364514 2201 8 0.04615718525139556 2201 9 0.13506853675016117 2201 10-11 0.2473705433230009 2201 12-13 0.21857696267404236 2201 14-15 0.291045651629922 2201 16-17 0.2545929666554514 2201 18-19 0.37228929147583045 2201 20-21 0.44012589987899275 2201 22-23 0.36620216807911987 2201 24-25 0.5970520998403614 2201 26-27 0.3493489708660604 2201 28-29 0.38458883571528446 2201 30-31 0.3223832688087711 2201 32-33 0.20578767771038997 2201 34-35 0.22381518127604494 2201 36-37 0.37524373634191477 2201 38-39 0.3950748785810063 2201 40-41 0.2813628756873001 2201 42-43 0.4452394229665515 2201 44-45 0.5202812659679026 2201 46-47 0.5495524473701536 2201 48-49 0.5661298908067351 2201 50-51 0.5066780449616672 2201 52-53 0.5365599335590048 2201 54-55 0.6810634042118835 2201 56-57 0.5658124961168483 2201 58-59 0.7069755797471586 2201 60-61 0.6989963445457903 2201 62-63 0.7236929096084168 2201 64-65 0.7482653547353664 2201 66-67 0.7769351283925232 2201 68-69 0.7142611952863049 2201 70-71 0.6556598949602161 2201 72-73 0.6477296535730872 2201 74-75 0.45435228678918094 2201 76-77 0.5520166921518239 2201 78-79 0.5929605836886189 2201 80-81 0.47735949472271955 2201 82-83 0.6008424999182722 2201 84-85 0.5595121995770853 2201 86-87 0.6317156435685405 2201 88-89 0.7177330587390607 2201 90-91 0.5374954842796846 2201 92-93 0.6635358231948452 2201 94-95 0.666177703598585 2201 96-97 0.7281729305626712 2201 98-99 0.5962838445658392 2201 100-101 0.38808299460223594 1101 1 0.27293267830305723 1101 2 0.27855577370758766 1101 3 0.24511573629098748 1101 4 0.4078891335967043 1101 5 0.47809273030920707 1101 6 0.35364023598181404 1101 7 0.5144627911311375 1101 8 0.2732676774409626 1101 9 0.40767408405054795 1101 10-11 0.3741589110557513 1101 12-13 0.41276424349067753 1101 14-15 0.6052496848200306 1101 16-17 0.6415211449601728 1101 18-19 0.64001132309442 1101 20-21 0.7380318099745935 1101 22-23 0.4551328082393553 1101 24-25 0.6828915899734938 1101 26-27 0.6773384872342021 1101 28-29 0.6861084350413833 1101 30-31 0.6488345576955865 1101 32-33 0.5333222953923595 1101 34-35 0.5246925198442725 1101 36-37 0.44724825550643743 1101 38-39 0.5937272979504868 1101 40-41 0.30169868384236764 1101 42-43 0.5744799215268657 1101 44-45 0.6627425682445178 1101 46-47 0.5169557689733679 1101 48-49 0.5197198518302315 1101 50-51 0.4966942613974936 1101 52-53 0.6484105521780066 1101 54-55 0.875441936970212 1101 56-57 0.8227170153678145 1101 58-59 0.8148455647322592 1101 60-61 0.8745442327353743 1101 62-63 0.8058767863565919 1101 64-65 0.7505541110229075 1101 66-67 0.6943618324365204 1101 68-69 0.7130365345477969 1101 70-71 0.6796480749419089 1101 72-73 0.8109326071081568 1101 74-75 0.7770764319038079 1101 76-77 0.6861307656150473 1101 78-79 0.7012435432054467 1101 80-81 0.9655038192727261 1101 82-83 0.8225612000793134 1101 84-85 0.7267314438877186 1101 86-87 0.7495354838072572 1101 88-89 0.8524290999751436 1101 90-91 0.8876473188720375 1101 92-93 0.74567086046412 1101 94-95 0.7220323469364054 1101 96-97 0.751485785891493 1101 98-99 0.693929522358399 1101 100-101 0.583309302916792 2304 1 0.2690756062638471 2304 2 0.19290827509499664 2304 3 0.2440260754420791 2304 4 0.39545234639702187 2304 5 0.2598807406103205 2304 6 0.34003623338782774 2304 7 0.42725952495458586 2304 8 0.30559233401852737 2304 9 0.44054668882323256 2304 10-11 0.5002322036086468 2304 12-13 0.47529132912336536 2304 14-15 0.6569731105017311 2304 16-17 0.4717998424803014 2304 18-19 0.4432938289714201 2304 20-21 0.6462528256195625 2304 22-23 0.5239634415832839 2304 24-25 0.4350857210819825 2304 26-27 0.3884583281031837 2304 28-29 0.40860424163983566 2304 30-31 0.47619424242662234 2304 32-33 0.4527781976440153 2304 34-35 0.3455247619578756 2304 36-37 0.4244240307401128 2304 38-39 0.3528760062580929 2304 40-41 0.2686234239761802 2304 42-43 0.3751916306263965 2304 44-45 0.4934932182996974 2304 46-47 0.3488483025361617 2304 48-49 0.31710256129765924 2304 50-51 0.2622310869457536 2304 52-53 0.34876960244270094 2304 54-55 0.6572007181712962 2304 56-57 0.5852475305482372 2304 58-59 0.5407175272441833 2304 60-61 0.5334704491335955 2304 62-63 0.5140967107818284 2304 64-65 0.5231252117830252 2304 66-67 0.46459928185068833 2304 68-69 0.5112972861799534 2304 70-71 0.5719616400503185 2304 72-73 0.585048758095553 2304 74-75 0.4894909270432066 2304 76-77 0.4198217072441395 2304 78-79 0.5242742033622072 2304 80-81 0.5028457482997908 2304 82-83 0.478013245648647 2304 84-85 0.4869947552095901 2304 86-87 0.5178274443238102 2304 88-89 0.7250566713565014 2304 90-91 0.39279339422231985 2304 92-93 0.47849077491249403 2304 94-95 0.5369595314024274 2304 96-97 0.5143610095915392 2304 98-99 0.423924346859085 2304 100-101 0.493479314951383 2202 1 0.09890570335122462 2202 2 0.0500371621078024 2202 3 0.015011019863074182 2202 4 0.0687631667150157 2202 5 0.03355669389581806 2202 6 -0.007299140187519981 2202 7 0.26313331136235263 2202 8 -0.004301784456217206 2202 9 -0.15459225869329885 2202 10-11 0.015048581827301177 2202 12-13 0.04891012045585086 2202 14-15 0.029653918156149928 2202 16-17 0.06344681025854726 2202 18-19 0.07055445230053436 2202 20-21 0.19820829206110346 2202 22-23 0.06357825796150252 2202 24-25 0.20479938366816697 2202 26-27 0.15152854056977105 2202 28-29 0.26040739346198194 2202 30-31 0.20084036596679056 2202 32-33 0.17610132976727044 2202 34-35 0.11325692742853732 2202 36-37 0.15232099851836267 2202 38-39 0.1267640743599614 2202 40-41 0.11925976842531583 2202 42-43 0.11591802713687827 2202 44-45 0.26969238813226326 2202 46-47 0.24422504377719179 2202 48-49 0.2755094136967031 2202 50-51 0.3161151097401884 2202 52-53 0.36350982335166293 2202 54-55 0.43830139356175124 2202 56-57 0.6344720626996363 2202 58-59 0.51021626088707 2202 60-61 0.6385955018984788 2202 62-63 0.6192553571867805 2202 64-65 0.4162277867091575 2202 66-67 0.6020510899272367 2202 68-69 0.5575988198791251 2202 70-71 0.5100510237565246 2202 72-73 0.5359645805241442 2202 74-75 0.5557572841559093 2202 76-77 0.4593278279701849 2202 78-79 0.43158952628327185 2202 80-81 0.5669061816753356 2202 82-83 0.485501637375112 2202 84-85 0.49100945902273097 2202 86-87 0.6737933933881131 2202 88-89 0.6040975817265597 2202 90-91 0.5699678349145962 2202 92-93 0.2887028897288708 2202 94-95 0.4469598831682866 2202 96-97 0.4634474736410681 2202 98-99 0.577820505575847 2202 100-101 0.4408586592598134 1102 1 -0.03166797493898699 1102 2 0.12915031644176977 1102 3 0.11691969432224525 1102 4 0.2836380464854784 1102 5 0.10910453302462031 1102 6 0.13444292466306962 1102 7 0.28747526861769046 1102 8 0.2824760639649071 1102 9 0.4181962514385873 1102 10-11 0.3738029223173527 1102 12-13 0.2261387277495075 1102 14-15 0.2383513635841652 1102 16-17 0.3182114864131549 1102 18-19 0.4248227231145094 1102 20-21 0.46940895719534836 1102 22-23 0.23509094930987118 1102 24-25 0.15279925324968957 1102 26-27 -0.013667983777729376 1102 28-29 0.1399444078569232 1102 30-31 0.28070896934789147 1102 32-33 0.16356306009445376 1102 34-35 0.18670731134793783 1102 36-37 0.17399231134338322 1102 38-39 0.1904901303421056 1102 40-41 0.135369222134905 1102 42-43 0.012029274100569864 1102 44-45 0.1866707549668405 1102 46-47 0.223391669688084 1102 48-49 0.15550435998058987 1102 50-51 0.193245740150509 1102 52-53 0.3145088289107534 1102 54-55 0.430299241656833 1102 56-57 0.5352796791388883 1102 58-59 0.5237101719742654 1102 60-61 0.4407483849620064 1102 62-63 0.5805023208815356 1102 64-65 0.5863930921329299 1102 66-67 0.4374497629191936 1102 68-69 0.37815570678999677 1102 70-71 0.40169185126178064 1102 72-73 0.416921526123339 1102 74-75 0.5183591328378654 1102 76-77 0.31605888876150345 1102 78-79 0.24567765657132767 1102 80-81 0.37259617694767044 1102 82-83 0.5297076333647439 1102 84-85 0.4640395693893744 1102 86-87 0.36374627971210316 1102 88-89 0.5451443530540558 1102 90-91 0.45780419232643865 1102 92-93 0.40501383999052365 1102 94-95 0.4720323469364054 1102 96-97 0.6403828023771467 1102 98-99 0.6596642968410684 1102 100-101 0.44453564528873457 2305 1 0.022262346707869085 2305 2 -0.08362297931153861 2305 3 0.016360350576952953 2305 4 -0.035813549768050734 2305 5 -0.06956809658683483 2305 6 -0.055871303809851725 2305 7 0.0290504711256645 2305 8 -0.004165822313566991 2305 9 -0.12441770637828142 2305 10-11 0.039142882175781324 2305 12-13 0.08354600853655825 2305 14-15 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1213 58-59 -1.257983956669051 1213 60-61 -1.1323930599237286 1213 62-63 -1.220205257464194 1213 64-65 -1.2123521651647984 1213 66-67 -0.9987445281848615 1213 68-69 -0.8820032062495855 1213 70-71 -1.7203118994066138 1213 72-73 -1.1274400628706083 1213 74-75 -0.9205679952059249 1213 76-77 -1.020402204663231 1213 78-79 -1.1195737396955714 1213 80-81 -1.093987226813745 1213 82-83 -1.175589439527677 1213 84-85 -1.2779092431121732 1213 86-87 -1.2228465732093312 1213 88-89 -1.2879347000855326 1213 90-91 -1.2378612187162865 1213 92-93 -1.4095233686355009 1213 94-95 -1.462198130698166 1213 96-97 -1.2838804317769572 1213 98-99 -1.2338316024111045 1213 100-101 -0.9718546391326903 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 204.0 3 99.0 4 111.0 5 127.0 6 323.0 7 2230.0 8 13467.0 9 23661.0 10 20495.0 11 14754.0 12 9942.0 13 8406.0 14 8478.0 15 9575.0 16 9749.0 17 9043.0 18 8743.0 19 8889.0 20 9028.0 21 9720.0 22 10250.0 23 11094.0 24 12013.0 25 13643.0 26 16026.0 27 19090.0 28 22256.0 29 26905.0 30 33935.0 31 44568.0 32 60750.0 33 86213.0 34 133233.0 35 234078.0 36 426523.0 37 475375.0 38 71129.0 39 34.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.48864081525399 22.553719483458963 24.488195873324816 12.469443827962236 2 34.59011830650508 21.956071093515764 31.865328961720447 11.588481638258704 3 39.614819955833 16.470604666703554 27.684842533537534 16.22973284392591 4 32.85630259097416 15.003263225463364 34.944337162363595 17.19609702119888 5 31.71125022695555 14.340528943413133 37.439178598172106 16.50904223145921 6 27.358141468347853 14.650287361106113 42.430944448890216 15.560626721655819 7 38.020157958237554 15.388229431950649 29.277471809088425 17.314140800723372 8 32.61427335839843 28.56378954986452 22.875180458462474 15.94675663327458 9 33.794052810220585 27.32978845631415 20.924366770214014 17.951791963251253 10-11 28.072489218217793 25.580863567815122 27.44140370766092 18.905243506306157 12-13 29.478672680446966 21.82954465532524 26.109665410544658 22.582117253683137 14-15 27.14720488407385 23.603690329917125 26.35855216267432 22.890552623334703 16-17 27.139431181509533 25.005986037232613 27.63780233514507 20.216780446112782 18-19 27.172127748267812 24.99678565168597 27.166141947232074 20.664944652814146 20-21 25.90479580157999 26.4255184142547 27.21529893772587 20.454386846439437 22-23 28.027499771130554 25.647203859718736 25.49777426143629 20.827522107714415 24-25 26.33666657597474 26.808623778723188 25.68675564712148 21.167953998180593 26-27 23.933987004922034 28.246422911012576 26.149400633697645 21.670189450367747 28-29 24.802532270361272 27.49622493100412 25.365751349504922 22.335491449129684 30-31 26.113856029472526 25.959519899306493 26.202785470090756 21.723838601130222 32-33 24.93962872607738 25.393233469296433 27.74881570115655 21.918322103469638 34-35 23.82466642396488 26.3169484534752 26.70444676630346 23.15393835625646 36-37 24.762122170310523 27.715576951205872 26.169411390172343 21.352889488311263 38-39 25.665360587977442 26.812917001702296 24.820694310355492 22.70102809996477 40-41 25.21944285181046 25.983700989751803 24.26634553428418 24.53051062415356 42-43 26.76683052796514 25.603975709972225 25.28328278514892 22.345910976913714 44-45 25.050034503772288 26.46199986338335 25.548012953840242 22.93995267900412 46-47 25.741259721246053 26.719591022441207 25.218686848717198 22.320462407595542 48-49 24.202024733232804 26.6064727671148 25.876760910917497 23.314741588734904 50-51 23.62328200242457 25.750899799422932 25.739199246430594 24.886618951721903 52-53 26.343315819287756 25.520404930041547 24.360303385516833 23.775975865153864 54-55 26.898172089247158 25.367656846617976 25.010373480248166 22.7237975838867 56-57 27.267582890027647 25.160801691406526 24.6296955236951 22.941919894870725 58-59 26.17495328047822 25.115134812276136 25.032681478684633 23.677230428561018 60-61 24.52779509731248 25.27409003881637 24.88915273732445 25.308962126546692 62-63 26.135763622657414 25.047531839342383 25.196660296826657 23.62004424117354 64-65 25.321569147213 26.150249042181645 25.78048316898548 22.747698641619873 66-67 24.298073373503986 26.102983384772237 25.186896778129427 24.41204646359435 68-69 23.55303525286828 24.553887602745803 28.244293692342026 23.648783452043894 70-71 23.522190961771887 26.63200386058347 26.73139506592699 23.11441011171765 72-73 23.65320093860543 28.467760774432815 25.25151297571739 22.62752531124437 74-75 24.074245338360377 28.48454167556096 25.17091827270249 22.270294713376174 76-77 24.068741948041612 27.99987202897522 24.926147814061327 23.00523820892184 78-79 24.007671976235585 28.205723816921097 25.02562836889043 22.760975837952895 80-81 24.24232647092274 28.682822087374138 24.857477871192742 22.217373570510375 82-83 23.8233435261908 28.308658012150744 25.06958843984735 22.7984100218111 84-85 23.445442375615222 28.370922034259294 25.074396638288466 23.109238951837018 86-87 23.22554585763491 28.60444065324352 24.997418349938364 23.17259513918321 88-89 23.367666729234447 28.64879953230138 25.101932591376297 22.88160114708788 90-91 23.80265802617692 27.986958596906362 24.81606915977063 23.394314217146082 92-93 23.44292073688888 27.772910432574214 24.512951096039814 24.27121773449709 94-95 23.502995420508114 28.3783060853342 23.994494394255675 24.12420409990201 96-97 23.435996305380318 28.87523776856154 23.714168049281668 23.97459787677648 98-99 23.62397451640841 27.690712185935357 24.373053796094162 24.31225950156207 100-101 24.356332555037138 27.87721976429538 23.91775104220251 23.84869663846497 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2201.0 1 1431.5 2 627.0 3 1048.0 4 1262.0 5 1030.5 6 1312.5 7 1862.5 8 2183.0 9 2095.0 10 1855.5 11 1613.0 12 1459.0 13 1360.0 14 1218.5 15 1165.0 16 1147.0 17 1089.5 18 1071.0 19 1024.0 20 987.0 21 973.0 22 998.0 23 1207.0 24 1390.5 25 1548.5 26 1809.0 27 2106.0 28 2518.0 29 3117.5 30 3936.5 31 4970.0 32 6463.5 33 8201.5 34 10786.0 35 15528.5 36 21199.5 37 28555.5 38 36552.5 39 41206.0 40 46571.0 41 50484.0 42 56962.0 43 69850.5 44 76979.0 45 82516.0 46 94822.0 47 101989.5 48 99498.5 49 96547.5 50 93612.0 51 91121.0 52 88850.5 53 86038.0 54 80636.0 55 70895.0 56 61669.0 57 55482.0 58 50602.5 59 42802.0 60 32746.5 61 24209.5 62 17524.5 63 13020.0 64 10288.5 65 8541.0 66 7761.5 67 7155.5 68 6038.0 69 4530.5 70 3098.0 71 2214.5 72 1914.5 73 1562.0 74 1063.5 75 743.0 76 573.5 77 460.5 78 365.5 79 293.0 80 238.5 81 178.5 82 149.0 83 127.5 84 96.0 85 65.5 86 56.5 87 53.0 88 34.0 89 29.0 90 20.0 91 17.5 92 15.0 93 9.0 94 8.5 95 4.5 96 5.0 97 5.5 98 2.5 99 0.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17391220384098138 2 0.16420273163394325 3 0.13882935951278835 4 0.13432330611283694 5 0.13738098520566108 6 0.11887398017014644 7 0.09484169537040564 8 0.08271826598482211 9 0.08395206632052309 10-11 0.063218856331461 12-13 0.08304012694196149 14-15 0.08666106270977958 16-17 0.07998244784913733 18-19 0.07603965112417986 20-21 0.07311608076349711 22-23 0.09352742979541981 24-25 0.4322860871846232 26-27 0.27199933052920916 28-29 0.12190483751654231 30-31 0.22838717083682242 32-33 0.259151713990062 34-35 0.2434341705830887 36-37 0.24627727570448657 38-39 0.2630676889685912 40-41 0.26312133246144775 42-43 0.28814602187903504 44-45 0.26510614169714064 46-47 0.2832108205362311 48-49 0.26824428602924966 50-51 0.28283531608623513 52-53 0.2827012073540937 54-55 0.260197762100765 56-57 0.274359644214898 58-59 0.2642210240650073 60-61 0.24169075706525034 62-63 0.2335101244046243 64-65 0.2324640762939213 66-67 0.24190533103667658 68-69 0.24643820618305626 70-71 0.23262500677249096 72-73 0.22345196949401847 74-75 0.23345648091176774 76-77 0.2340465593331899 78-79 0.23621912079388077 80-81 0.24330006185094727 82-83 0.26896847318281325 84-85 0.26113652322575487 86-87 0.26194117561860336 88-89 0.2597417924114842 90-91 0.25193666420085414 92-93 0.22844081432967897 94-95 0.22726065748683455 96-97 0.2229959998047377 98-99 0.247269680322333 100-101 0.2551016302793914 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1864159.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.018963128904836 #Duplication Level Percentage of deduplicated Percentage of total 1 84.07160321110155 43.7331762762621 2 7.321009413054176 7.616626374480609 3 2.5470642864608473 3.974869296130714 4 1.4866881594341583 3.093439061991395 5 0.9030855215167258 2.348878622301318 6 0.6222913465398798 1.9422570366656733 7 0.40824886988167064 1.4865678036854186 8 0.3239350988129369 1.348061436904665 9 0.28859937051053247 1.3511376012251282 >10 1.8130550131150254 17.88235609304973 >50 0.12867786317981503 4.615772336854838 >100 0.08104156782280023 7.531247239127163 >500 0.0036751904475129395 1.2913455441185087 >1k 8.202347475883024E-4 1.0739722573445587 >5k 2.048533746624758E-4 0.7102930198582632 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6842 0.36702877812461276 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6489 0.34809262514624556 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 4746 0.25459201709725404 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 4082 0.21897273784049534 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT 3954 0.21210637075485514 Illumina Single End PCR Primer 1 (96% over 32bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.002896748614254471 0.0 0.0 2 0.0032186095713938566 0.0 0.0 3 0.003486827035676678 0.0 0.0 4 0.0038623314856726276 0.0 0.0 5 0.005096131821373606 0.0 0.0 6 0.006008071199935199 0.0 0.0 7 0.007080941057066484 0.0 0.0 8 0.007939236942771512 0.0 0.0 9 0.009763115699894697 0.0 0.0 10-11 0.018721579006940933 0.0 0.0 12-13 0.03146190856037495 0.0 0.0 14-15 0.04476549478880289 0.0 0.0 16-17 0.057023032906527826 0.0 0.0 18-19 0.06767126623855584 0.0 0.0 20-21 0.07247235884921832 0.0 0.0 22-23 0.07772942114916163 0.0 0.0 24-25 0.08494447093836953 0.0 0.0 26-27 0.09261549041685822 0.0 0.0 28-29 0.10184217118818728 0.0 0.0 30-31 0.15881156060185853 0.0 0.0 32-33 0.16940615044102997 0.0 0.0 34-35 0.17635298276595507 0.0 0.0 36-37 0.18388989351230234 0.0 0.0 38-39 0.19072943885151428 0.0 0.0 40-41 0.19869549754071408 0.0 0.0 42-43 0.20746620862276233 0.0 0.0 44-45 0.21677335463337621 0.0 0.0 46-47 0.22645600509398608 0.0 0.0 48-49 0.23777478208672115 0.0 0.0 50-51 0.24874487637588855 0.0 0.0 52-53 0.261431562436466 0.0 0.0 54-55 0.2759421272541666 0.0 0.0 56-57 0.29039904857901067 0.0 0.0 58-59 0.3072431053359719 0.0 0.0 60-61 0.3267156932429047 0.0 0.0 62-63 0.3472879727533971 0.0 0.0 64-65 0.3695500222888713 0.0 0.0 66-67 0.39390416804575146 0.0 0.0 68-69 0.4200821925597549 0.0 0.0 70-71 0.44977386585586315 0.0 0.0 72-73 0.48308647491978957 0.0 0.0 74-75 0.5227290161407906 0.0 0.0 76-77 0.5685137372938682 0.0 0.0 78-79 0.6181339681861902 0.0 0.0 80-81 0.673547696307021 0.0 0.0 82-83 0.7359887219920618 0.0 0.0 84-85 0.8042500666520399 0.0 0.0 86-87 0.8798069263405106 0.0 5.364349285656427E-5 88-89 0.9644295363217408 0.0 5.364349285656427E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 1155 0.0 65.67333 9 CGGGAGA 1715 0.0 57.247364 4 AGAGCGG 1480 0.0 52.853443 8 CGGAAAG 780 0.0 51.07801 5 TCGGGAG 1925 0.0 51.00631 3 ATCGGGA 2125 0.0 50.905556 2 ATTGGGA 1030 0.0 50.66928 2 GGAGAGC 1730 0.0 50.434498 6 GATTGGG 1165 0.0 46.43547 1 GATCGGG 2620 0.0 43.650246 1 AGAGCGT 3795 0.0 43.098118 8 TCGGAGA 685 0.0 42.23656 4 GAGCGTC 4055 0.0 40.68548 9 TGTGTTT 41635 0.0 40.541164 1 GTACGGA 130 5.456968E-12 40.14566 2 GAGGGGC 2355 0.0 39.25498 9 AAGAGCG 4150 0.0 38.269054 7 CGGAAGA 4410 0.0 37.750076 4 TTGTGTT 23160 0.0 37.74175 1 AAGCGGC 415 0.0 37.697872 9 >>END_MODULE