FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n02_40825-1.342000000356f6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n02_40825-1.342000000356f6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1864159
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA68420.36702877812461276No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64890.34809262514624556No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA47460.25459201709725404No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC40820.21897273784049534No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT39540.21210637075485514Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC11550.065.673339
CGGGAGA17150.057.2473644
AGAGCGG14800.052.8534438
CGGAAAG7800.051.078015
TCGGGAG19250.051.006313
ATCGGGA21250.050.9055562
ATTGGGA10300.050.669282
GGAGAGC17300.050.4344986
GATTGGG11650.046.435471
GATCGGG26200.043.6502461
AGAGCGT37950.043.0981188
TCGGAGA6850.042.236564
GAGCGTC40550.040.685489
TGTGTTT416350.040.5411641
GTACGGA1305.456968E-1240.145662
GAGGGGC23550.039.254989
AAGAGCG41500.038.2690547
CGGAAGA44100.037.7500764
TTGTGTT231600.037.741751
AAGCGGC4150.037.6978729
Produced by FastQC (version 0.11.2)