##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n02_40255-1.342000000357f5.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7760650 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.848001520491195 33.0 33.0 33.0 27.0 33.0 2 31.6842046735776 33.0 33.0 33.0 27.0 33.0 3 31.64221347438681 33.0 33.0 33.0 27.0 33.0 4 35.305387306475616 37.0 37.0 37.0 33.0 37.0 5 35.33360453054834 37.0 37.0 37.0 33.0 37.0 6 35.496640874153584 37.0 37.0 37.0 33.0 37.0 7 35.26929161861442 37.0 37.0 37.0 33.0 37.0 8 35.33199474270841 37.0 37.0 37.0 33.0 37.0 9 35.147440742721294 37.0 37.0 37.0 33.0 37.0 10-11 35.10439325314246 37.0 37.0 37.0 33.0 37.0 12-13 35.09922731987656 37.0 37.0 37.0 33.0 37.0 14-15 36.82327227745098 40.0 37.0 40.0 33.0 40.0 16-17 36.75510157010044 40.0 37.0 40.0 33.0 40.0 18-19 36.72711441696249 40.0 37.0 40.0 33.0 40.0 20-21 36.63808134627898 40.0 37.0 40.0 33.0 40.0 22-23 36.89234819248388 40.0 37.0 40.0 33.0 40.0 24-25 36.55258747656446 40.0 37.0 40.0 33.0 40.0 26-27 36.487454401371025 40.0 37.0 40.0 33.0 40.0 28-29 36.39814454974777 40.0 37.0 40.0 33.0 40.0 30-31 36.34498096164626 40.0 37.0 40.0 33.0 40.0 32-33 36.25585060529724 40.0 37.0 40.0 33.0 40.0 34-35 36.22668532919279 40.0 37.0 40.0 33.0 40.0 36-37 36.1107897534356 40.0 37.0 40.0 33.0 40.0 38-39 36.01276967779761 40.0 37.0 40.0 30.0 40.0 40-41 35.950646853034215 38.5 37.0 40.0 27.0 40.0 42-43 35.84703362476081 37.0 37.0 40.0 27.0 40.0 44-45 35.66570744718548 37.0 37.0 40.0 27.0 40.0 46-47 35.566122038746755 37.0 37.0 40.0 27.0 40.0 48-49 35.44611881736711 37.0 37.0 40.0 27.0 40.0 50-51 34.289691005263734 37.0 35.0 38.5 24.5 38.5 52-53 34.70195338019367 37.0 35.0 38.5 27.0 40.0 54-55 35.43886246641712 37.0 37.0 40.0 27.0 40.0 56-57 35.52231862021867 37.0 37.0 40.0 27.0 40.0 58-59 35.24928369402048 37.0 37.0 40.0 27.0 40.0 60-61 35.025543543388764 37.0 37.0 40.0 27.0 40.0 62-63 34.749400952239824 37.0 33.0 40.0 27.0 40.0 64-65 34.525836624509544 37.0 33.0 40.0 27.0 40.0 66-67 34.311672475887974 37.0 33.0 37.0 27.0 40.0 68-69 34.1830682352638 37.0 33.0 37.0 27.0 40.0 70-71 33.80083156694349 37.0 33.0 37.0 27.0 40.0 72-73 33.7000249979061 37.0 33.0 37.0 27.0 38.5 74-75 33.538042689723156 37.0 33.0 37.0 27.0 37.0 76-77 33.26970260223048 37.0 33.0 37.0 27.0 37.0 78-79 32.99075141901774 37.0 33.0 37.0 22.0 37.0 80-81 32.72111537049088 37.0 33.0 37.0 22.0 37.0 82-83 32.466859219266425 37.0 33.0 37.0 22.0 37.0 84-85 32.28518390856436 37.0 33.0 37.0 22.0 37.0 86-87 32.140557234252285 37.0 33.0 37.0 22.0 37.0 88-89 32.00618066785643 33.0 33.0 37.0 22.0 37.0 90-91 31.855962000605622 33.0 33.0 37.0 22.0 37.0 92-93 31.704493180339277 33.0 33.0 37.0 15.0 37.0 94-95 31.587780662702222 33.0 33.0 37.0 15.0 37.0 96-97 31.45287856042986 33.0 33.0 37.0 15.0 37.0 98-99 31.30931378170643 33.0 33.0 37.0 15.0 37.0 100-101 30.043352296521554 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.21587334754399734 2306 2 0.24649486857244796 2306 3 0.23959061273557936 2306 4 0.3604293349168728 2306 5 0.3285273688006427 2306 6 0.2007783295512695 2306 7 0.36474712559227385 2306 8 0.2073034241325331 2306 9 0.38275140326171453 2306 10-11 0.33079987998840465 2306 12-13 0.3808684985209041 2306 14-15 0.5779909056984636 2306 16-17 0.5226950954970988 2306 18-19 0.49860726813039946 2306 20-21 0.5679920212803751 2306 22-23 0.4439316754792557 2306 24-25 0.45974505303122015 2306 26-27 0.3885237563008488 2306 28-29 0.35768401770341285 2306 30-31 0.3429247330469778 2306 32-33 0.38565570381418723 2306 34-35 0.37701444706313225 2306 36-37 0.346904460461154 2306 38-39 0.2511282087899076 2306 40-41 0.26306485368812815 2306 42-43 0.19671766136557522 2306 44-45 0.3306813930044967 2306 46-47 0.31718684316731327 2306 48-49 0.2617466969744413 2306 50-51 0.2376921182603624 2306 52-53 0.2931978776724691 2306 54-55 0.4662333145051676 2306 56-57 0.3291086726493546 2306 58-59 0.4097803688025863 2306 60-61 0.43295439529977386 2306 62-63 0.39320884139726786 2306 64-65 0.4131549613723209 2306 66-67 0.3676078817507289 2306 68-69 0.2777563097650031 2306 70-71 0.3389247920684184 2306 72-73 0.17291474126832185 2306 74-75 0.16718901835790945 2306 76-77 0.3047838032119756 2306 78-79 0.4162657826547189 2306 80-81 0.3452101699147576 2306 82-83 0.28517018320302157 2306 84-85 0.39702370042838453 2306 86-87 0.37187184466776557 2306 88-89 0.4206293062128452 2306 90-91 0.40009532160384254 2306 92-93 0.3022050375471679 2306 94-95 0.2938207259170724 2306 96-97 0.35320910299366304 2306 98-99 0.3361995485653928 2306 100-101 0.1780910616762057 2307 1 0.16612056898557626 2307 2 0.17732809944227412 2307 3 0.10099147345741244 2307 4 0.22458138513182035 2307 5 0.1759836971750275 2307 6 0.13304872195895 2307 7 0.32017720306461683 2307 8 0.1936891636531044 2307 9 0.23554557776261476 2307 10-11 0.2770128004083432 2307 12-13 0.27527100407468197 2307 14-15 0.4426894476563703 2307 16-17 0.41120872238666806 2307 18-19 0.5169334013478064 2307 20-21 0.6170081091033168 2307 22-23 0.36189991247809417 2307 24-25 0.429367349014548 2307 26-27 0.34085951273942783 2307 28-29 0.3517896936329947 2307 30-31 0.37531853219442013 2307 32-33 0.3365864212902636 2307 34-35 0.26612494013738797 2307 36-37 0.23558081098751416 2307 38-39 0.24998994151886222 2307 40-41 0.19308242656380514 2307 42-43 0.13567923410956695 2307 44-45 0.2465715246442599 2307 46-47 0.2646857485874463 2307 48-49 0.12235313146507565 2307 50-51 0.11255569043689917 2307 52-53 0.0818046369718175 2307 54-55 0.1774965572186602 2307 56-57 0.17475512182945607 2307 58-59 0.2878354988754239 2307 60-61 0.3030399516773201 2307 62-63 0.24460626033858546 2307 64-65 0.2505669098635366 2307 66-67 0.19754225875261966 2307 68-69 0.19852242130294684 2307 70-71 0.09615696552904751 2307 72-73 0.06292810752336209 2307 74-75 0.10285794731000664 2307 76-77 0.3036688075140219 2307 78-79 0.19564183346734865 2307 80-81 0.22776820747976245 2307 82-83 0.22631272094962185 2307 84-85 0.13311960953415536 2307 86-87 0.20310966034966782 2307 88-89 0.31790813917750427 2307 90-91 0.33986455273874583 2307 92-93 0.247702463519623 2307 94-95 0.20940896565599232 2307 96-97 0.23737078607896578 2307 98-99 0.2828784503089068 2307 100-101 0.159035545564862 2201 1 0.17140114612287505 2201 2 0.1697520746399661 2201 3 0.15814018536084262 2201 4 0.2389194575956637 2201 5 0.2330440569835801 2201 6 0.13577488608648736 2201 7 0.31511476189489684 2201 8 0.22966764268919349 2201 9 0.2912818609492831 2201 10-11 0.23750549184943281 2201 12-13 0.26035236798738737 2201 14-15 0.41386337190100875 2201 16-17 0.3623664293365536 2201 18-19 0.36845273609937834 2201 20-21 0.4577661419653438 2201 22-23 0.3381603237182773 2201 24-25 0.3884509416757851 2201 26-27 0.33017764406852734 2201 28-29 0.26953938184045967 2201 30-31 0.2566615516393185 2201 32-33 0.24125056184559668 2201 34-35 0.241498969025713 2201 36-37 0.27565369008711116 2201 38-39 0.20601824176578987 2201 40-41 0.2669090354437387 2201 42-43 0.2669425024021521 2201 44-45 0.33379612631186006 2201 46-47 0.31198102657626947 2201 48-49 0.2971727442646994 2201 50-51 0.30502209472015807 2201 52-53 0.33769576389809686 2201 54-55 0.41306964739274576 2201 56-57 0.419587427880046 2201 58-59 0.44834006430259876 2201 60-61 0.4168702305158618 2201 62-63 0.4080154678182879 2201 64-65 0.4316678880751752 2201 66-67 0.49440467840994984 2201 68-69 0.34128166927401793 2201 70-71 0.4612897182329405 2201 72-73 0.4219692342856618 2201 74-75 0.37646679839610186 2201 76-77 0.42387437606544154 2201 78-79 0.46588957341072046 2201 80-81 0.4114128726229822 2201 82-83 0.40188210502105903 2201 84-85 0.3976827623063741 2201 86-87 0.3688112365577325 2201 88-89 0.38526360970830353 2201 90-91 0.33856289880760926 2201 92-93 0.36706104002814754 2201 94-95 0.3874199297493668 2201 96-97 0.3992631821964814 2201 98-99 0.3733135127574414 2201 100-101 0.20210540134947763 1101 1 0.155832137036775 1101 2 0.17896750904012393 1101 3 0.12042933235010267 1101 4 0.24043753537634416 1101 5 0.2598401798298511 1101 6 0.18044757434137892 1101 7 0.33021614220441364 1101 8 0.1883292943369952 1101 9 0.2486765249641607 1101 10-11 0.2165044396846838 1101 12-13 0.23265968055604702 1101 14-15 0.3300715308398807 1101 16-17 0.3444195776188934 1101 18-19 0.36051174394067687 1101 20-21 0.4035349298270674 1101 22-23 0.18075644202077967 1101 24-25 0.2533118753619519 1101 26-27 0.2523001770072426 1101 28-29 0.29191314691946957 1101 30-31 0.3058040764181982 1101 32-33 0.20093414566166246 1101 34-35 0.17223926608357942 1101 36-37 0.2388208243036658 1101 38-39 0.2803125565736764 1101 40-41 0.1381301701345521 1101 42-43 0.21099897851640037 1101 44-45 0.2037355712251454 1101 46-47 0.1384141928159366 1101 48-49 0.2924386537008985 1101 50-51 0.3068945326421968 1101 52-53 0.32031445664848235 1101 54-55 0.4077955710187453 1101 56-57 0.35133968804635174 1101 58-59 0.47291619289482867 1101 60-61 0.4907971100356576 1101 62-63 0.4050361980196726 1101 64-65 0.501141733521969 1101 66-67 0.36355393481866116 1101 68-69 0.42702916180402894 1101 70-71 0.49490275518365934 1101 72-73 0.32269272179292585 1101 74-75 0.2492929517187008 1101 76-77 0.33882590099435816 1101 78-79 0.4651429292861593 1101 80-81 0.42081431725511464 1101 82-83 0.3304913306552635 1101 84-85 0.34491788941573276 1101 86-87 0.47561841234023916 1101 88-89 0.48568407921138146 1101 90-91 0.48881973373701726 1101 92-93 0.40087833916904714 1101 94-95 0.3044592479579684 1101 96-97 0.3464281709057069 1101 98-99 0.42708881050397096 1101 100-101 0.24932126480376837 2304 1 0.18239073536460637 2304 2 0.14728184172606618 2304 3 0.17837523330379312 2304 4 0.2286792542551055 2304 5 0.22609370492271097 2304 6 0.17149673722241232 2304 7 0.34096368965081325 2304 8 0.18008451106032197 2304 9 0.23461073151286627 2304 10-11 0.26617646628696434 2304 12-13 0.26472715528512225 2304 14-15 0.3839410061238553 2304 16-17 0.3036490057774017 2304 18-19 0.3839758180162889 2304 20-21 0.4556245179140106 2304 22-23 0.3802417059938179 2304 24-25 0.42998502626698354 2304 26-27 0.35893791134051156 2304 28-29 0.361602007004862 2304 30-31 0.3705831188352633 2304 32-33 0.33798426927284453 2304 34-35 0.18155275813063554 2304 36-37 0.34382666322401434 2304 38-39 0.2761547587162667 2304 40-41 0.30961630994563194 2304 42-43 0.2345695818300939 2304 44-45 0.34923794613352044 2304 46-47 0.31060695586757703 2304 48-49 0.2372573365895505 2304 50-51 0.26490394738360834 2304 52-53 0.28795420768685176 2304 54-55 0.45140940718940925 2304 56-57 0.45962055046730654 2304 58-59 0.47955071789245096 2304 60-61 0.38125409872638016 2304 62-63 0.47461868415742003 2304 64-65 0.44528950997000294 2304 66-67 0.3143434313877762 2304 68-69 0.33501435123765333 2304 70-71 0.4754995777343183 2304 72-73 0.3610360006158615 2304 74-75 0.38813315993211717 2304 76-77 0.45848882757718457 2304 78-79 0.3729272246609838 2304 80-81 0.4054922241885208 2304 82-83 0.3011796399772422 2304 84-85 0.25194576508399535 2304 86-87 0.4049975508303234 2304 88-89 0.45312096917879074 2304 90-91 0.2631900310250188 2304 92-93 0.3465362115658621 2304 94-95 0.3207092531343143 2304 96-97 0.38577690352870775 2304 98-99 0.440059288757098 2304 100-101 0.28906707506784457 2202 1 0.10559549361160947 2202 2 0.1311026946951266 2202 3 0.12818262441057016 2202 4 0.17272244939444192 2202 5 0.14965542396285514 2202 6 0.1495658503680275 2202 7 0.30547924945068416 2202 8 0.15423847361346077 2202 9 0.08170522059182161 2202 10-11 0.2100829898463843 2202 12-13 0.18973113433111877 2202 14-15 0.31170171437097594 2202 16-17 0.3552720528731541 2202 18-19 0.32251443552269876 2202 20-21 0.4234651761520283 2202 22-23 0.37408923587126 2202 24-25 0.15561504188330133 2202 26-27 0.2571047821495398 2202 28-29 0.1731238825310797 2202 30-31 0.1184243422131388 2202 32-33 0.11122864497783524 2202 34-35 0.02119339837643963 2202 36-37 0.08571038973321521 2202 38-39 0.16924765387012997 2202 40-41 0.07723812065376734 2202 42-43 0.030753042377561712 2202 44-45 0.11772620852449478 2202 46-47 0.20324462509237406 2202 48-49 0.14125973785731105 2202 50-51 0.04598802404886726 2202 52-53 0.1869439913420905 2202 54-55 0.2669219198473911 2202 56-57 0.2637940507129741 2202 58-59 0.17338158191056863 2202 60-61 0.30100383403332387 2202 62-63 0.30546084635948034 2202 64-65 0.23946412896192726 2202 66-67 0.2818841041579532 2202 68-69 0.19877660266261188 2202 70-71 0.27375294954329377 2202 72-73 0.28937263252066003 2202 74-75 0.22635287708538243 2202 76-77 0.2570290367020007 2202 78-79 0.32811272893874843 2202 80-81 0.3191648605775299 2202 82-83 0.3388635699368052 2202 84-85 0.34125741319331837 2202 86-87 0.3186090598460325 2202 88-89 0.338969809258046 2202 90-91 0.38809528359640666 2202 92-93 0.4207434587349752 2202 94-95 0.39939690609691425 2202 96-97 0.28645928108365837 2202 98-99 0.39365530832708373 2202 100-101 0.19935435065541895 1102 1 0.05539783059139225 1102 2 0.1036391170021318 1102 3 0.11382642393818188 1102 4 0.16823748543706785 1102 5 0.24264821748259635 1102 6 0.10735192227124202 1102 7 0.26130490100230475 1102 8 0.15339147056938884 1102 9 0.20492503894945457 1102 10-11 0.26512688683763486 1102 12-13 0.20085671669684046 1102 14-15 0.22915537695347865 1102 16-17 0.2312031349292596 1102 18-19 0.3457193224091313 1102 20-21 0.370543337801557 1102 22-23 0.15373922582319466 1102 24-25 0.1584442488636597 1102 26-27 0.14731781640345787 1102 28-29 0.05800924513827965 1102 30-31 0.18604268562777548 1102 32-33 0.148163479844456 1102 34-35 0.13805849509095935 1102 36-37 0.16242690808230975 1102 38-39 0.09723833295037565 1102 40-41 0.2178991757462896 1102 42-43 0.14854491300965833 1102 44-45 0.18702367769126482 1102 46-47 0.21664209216259422 1102 48-49 0.23419295889780045 1102 50-51 0.2058167416656076 1102 52-53 0.25685513374793345 1102 54-55 0.33055310023643614 1102 56-57 0.34367491598149513 1102 58-59 0.30191004962969714 1102 60-61 0.43355449237313337 1102 62-63 0.3647361791088457 1102 64-65 0.34940782402038195 1102 66-67 0.3660368531082767 1102 68-69 0.2713491775385677 1102 70-71 0.32034742356943724 1102 72-73 0.2347120448036364 1102 74-75 0.3483843139684666 1102 76-77 0.3236556645263917 1102 78-79 0.2936806540932224 1102 80-81 0.2873765765666292 1102 82-83 0.29133035636205307 1102 84-85 0.36024845482194934 1102 86-87 0.3244687418568333 1102 88-89 0.40317897053378715 1102 90-91 0.42144615629487703 1102 92-93 0.35460629680879663 1102 94-95 0.38790662766077944 1102 96-97 0.43529889611760453 1102 98-99 0.3425780180209337 1102 100-101 0.32262540645872306 2305 1 0.05236841986596019 2305 2 -0.056454728288915845 2305 3 -0.008934824526566132 2305 4 -0.01784740992091116 2305 5 0.028599784787431304 2305 6 0.04904970515444518 2305 7 0.10666888074452885 2305 8 -0.026747027006344126 2305 9 5.679864973515691E-4 2305 10-11 -0.045699905683193265 2305 12-13 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-0.9841536309647978 1213 66-67 -0.975130286365335 1213 68-69 -0.9272678515474908 1213 70-71 -1.7818231426172346 1213 72-73 -1.257621689933842 1213 74-75 -1.1115724159875882 1213 76-77 -1.1685743651937912 1213 78-79 -1.0696250850365914 1213 80-81 -1.0960281341423723 1213 82-83 -1.1930409793699823 1213 84-85 -1.1471756851359096 1213 86-87 -1.1667453787982538 1213 88-89 -1.2383242453050514 1213 90-91 -1.256938600904217 1213 92-93 -1.1901440654749749 1213 94-95 -1.2947090523728235 1213 96-97 -1.2572838131687654 1213 98-99 -1.1464152521445108 1213 100-101 -0.8966878290897853 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 805.0 3 349.0 4 439.0 5 409.0 6 1424.0 7 4764.0 8 18115.0 9 54109.0 10 73771.0 11 58120.0 12 35168.0 13 25737.0 14 22195.0 15 22509.0 16 23300.0 17 23380.0 18 24175.0 19 25944.0 20 28622.0 21 30920.0 22 33582.0 23 36228.0 24 40012.0 25 45345.0 26 53287.0 27 63163.0 28 77971.0 29 95957.0 30 117207.0 31 149748.0 32 204018.0 33 291176.0 34 441292.0 35 753597.0 36 1505902.0 37 2604409.0 38 772783.0 39 718.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.368625336843714 26.22454646642329 22.370760068131702 15.036068128601293 2 32.39487871107052 24.52218652074441 29.81737601194585 13.265558756239223 3 36.51354660906657 20.244168938662373 26.126533266417702 17.115751185853355 4 30.884490628458654 19.096534147209805 31.13480100015756 18.884174224173982 5 31.08132870767781 17.77684377985775 32.785740131553474 18.356087380910964 6 27.46454050318721 18.186650606127934 36.51361159042427 17.835197300260596 7 33.34872595526267 19.082616071548347 28.264014079347504 19.304643893841472 8 29.789654311864133 27.84004725507228 23.6600111044261 18.71028732863749 9 29.33750257138122 27.24940559983956 24.133246234175928 19.27984559460329 10-11 25.018877724439896 28.59794727957033 28.690646706481814 17.692528289507955 12-13 25.61829052203232 25.818008668661335 28.528251821684087 20.03544898762226 14-15 24.46362310416857 26.517485762663313 29.008136993653082 20.010754139515033 16-17 24.30742347986472 27.576575913730018 29.681706235082896 18.43429437132237 18-19 23.65516329304486 27.409288394352814 30.12868439679201 18.80686391581031 20-21 22.955167525099313 28.745088802143265 29.513148466237414 18.78659520652001 22-23 24.38865029297938 27.6300168559239 28.855167008824207 19.12616584227251 24-25 22.9130383149412 28.561200455221897 29.189722495203636 19.336038734633267 26-27 22.146654550282616 29.229066529092083 29.24777736176613 19.376501558859168 28-29 22.3881369189211 29.239585534007983 28.673802362617423 19.698475184453493 30-31 22.721551967253102 28.983777857237836 28.83847014178626 19.456200033722805 32-33 22.627210297778692 28.59527754052059 29.3669042310561 19.41060793064462 34-35 21.779415702678868 28.476914994470615 29.00734456847221 20.736324734378307 36-37 20.440317400874555 29.164875651845968 29.49103357966286 20.903773367616612 38-39 20.584575435113003 29.4548314155548 29.45399805756527 20.506595091766926 40-41 21.46089830879986 28.266106893337483 29.927512980148908 20.345481817713754 42-43 22.276606332441105 28.25729182424083 29.9515163209087 19.514585522409362 44-45 21.122455907938598 29.012897157705137 29.985806065411747 19.878840868944522 46-47 22.081725896688763 29.277056546985396 29.006732576280612 19.634484980045226 48-49 21.429900437825484 29.00059888479511 29.167408004641192 20.40209267273821 50-51 20.76021177537564 28.64393252228329 29.152784775889067 21.443070926452005 52-53 22.854726472827686 28.385787524921675 28.113949366290335 20.645536635960305 54-55 23.563822980343918 28.127651968343915 28.301602681145848 20.006922370166315 56-57 23.775439700968064 27.658205578381978 28.18852414425471 20.377830576395244 58-59 22.84272905286359 27.68321809743745 28.57899530647395 20.89505754322501 60-61 21.2519254753058 27.861280524233056 28.223583124666167 22.663210875794977 62-63 22.09259844439842 28.00903030870174 28.538689416937764 21.359681829962078 64-65 21.356868133358443 28.783122169292362 29.361101230354407 20.498908466994788 66-67 21.46309097785774 28.3184070013651 28.699840962615365 21.5186610581618 68-69 21.140339001792746 27.498805740403487 30.649745685357743 20.71110957244602 70-71 20.968445133676124 29.619673231768463 29.320155383239026 20.09172625131639 72-73 21.052201305744582 31.11592582066873 27.977052897940492 19.854819975646194 74-75 21.855626148166817 30.711165628830855 27.564437804958853 19.86877041804348 76-77 21.980517666782806 29.841913914656054 27.755094923659694 20.422473494901443 78-79 21.695772806454368 29.772207512018404 28.343219154323624 20.1888005272036 80-81 21.1268346117379 30.515648291915436 28.100748958201464 20.256768138145198 82-83 20.49973034134109 30.382177513698068 28.39632948954533 20.721762655415514 84-85 20.647929445331158 29.954835807653367 28.581679758078636 20.815554988936828 86-87 20.52905983441557 30.1282048383326 29.073917251195862 20.268818076055968 88-89 20.552824457659945 30.514510850677063 28.921153167865405 20.011511523797587 90-91 21.06855554967038 29.524140488041827 28.724971675798017 20.68233228648978 92-93 20.72560247845706 30.014443545086323 28.199281400374215 21.060672576082403 94-95 20.47433636730788 30.557500411035033 27.76773814749965 21.200425074157437 96-97 20.76948921454743 30.829187301043238 27.51416093318837 20.887162551220964 98-99 21.05910279272546 29.95352573201025 27.807311444252157 21.180060031012125 100-101 21.350730187275207 30.06418206622845 27.353090506015132 21.23199724048121 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2316.0 1 1663.5 2 1009.0 3 1312.5 4 1434.0 5 1297.0 6 1584.5 7 2060.5 8 2530.0 9 2892.5 10 3138.5 11 3348.5 12 3393.5 13 3338.5 14 3127.0 15 2868.0 16 2745.0 17 2627.5 18 2685.0 19 3023.5 20 3559.0 21 4429.5 22 5792.0 23 7638.5 24 10178.0 25 13536.5 26 18079.0 27 23792.0 28 30038.0 29 37268.0 30 46067.0 31 56599.5 32 71732.0 33 94253.5 34 126261.5 35 177952.0 36 247852.0 37 329703.0 38 411161.0 39 451946.5 40 469871.0 41 458211.0 42 433008.5 43 448348.5 44 450330.5 45 440836.0 46 457144.5 47 427614.5 48 343012.5 49 276044.5 50 232727.5 51 207243.5 52 188698.5 53 161288.0 54 127652.0 55 95447.5 56 73203.5 57 60610.0 58 50752.5 59 39110.0 60 28307.0 61 20815.5 62 15184.0 63 10936.5 64 7857.0 65 5511.5 66 4086.5 67 3145.5 68 2328.0 69 1559.0 70 1004.5 71 731.0 72 554.0 73 443.5 74 356.0 75 282.0 76 231.0 77 197.0 78 163.0 79 126.5 80 102.5 81 94.5 82 78.5 83 59.0 84 50.5 85 42.5 86 36.0 87 27.0 88 22.0 89 22.0 90 18.5 91 9.0 92 7.0 93 9.5 94 7.0 95 5.0 96 4.5 97 3.0 98 2.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18653076739706081 2 0.176454291844111 3 0.14933027517024994 4 0.1426555765303164 5 0.14697222526463632 6 0.12854593365246467 7 0.1033160882142604 8 0.09000534749022311 9 0.09064962342071863 10-11 0.07058687094508836 12-13 0.08985072126690419 14-15 0.09479231765380476 16-17 0.08709966304368835 18-19 0.08306649571878644 20-21 0.0812367520761792 22-23 0.10161519975775224 24-25 0.4477395578978565 26-27 0.2811298022717169 28-29 0.12515059949875335 30-31 0.23342761237782916 32-33 0.26509377436168363 34-35 0.2484714553548994 36-37 0.25077152042676837 38-39 0.26903674305631614 40-41 0.2686695057759337 42-43 0.29496884925876055 44-45 0.2715816329817734 46-47 0.29027852048475317 48-49 0.27401055323974155 50-51 0.28929277831109507 52-53 0.2887129299736491 54-55 0.26449459774632283 56-57 0.2810460464007525 58-59 0.2699065155624851 60-61 0.24553355711183986 62-63 0.23838853704264462 64-65 0.2361657850824351 66-67 0.24577838196542814 68-69 0.2506491079999742 70-71 0.23675207617918603 72-73 0.22822186285942542 74-75 0.23731259623871712 76-77 0.238478735672914 78-79 0.24056618968771945 80-81 0.24778852286857414 82-83 0.27363687320005414 84-85 0.2677546339546301 86-87 0.26713612906135437 88-89 0.26518397299195295 90-91 0.25667953070941224 92-93 0.2331119171718864 94-95 0.2319522204969945 96-97 0.22684311236816504 98-99 0.25106788735479635 100-101 0.2599009103618898 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7760650.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.30100131463484 #Duplication Level Percentage of deduplicated Percentage of total 1 89.26425358039815 40.43760068795514 2 5.609806821266412 5.082597323700745 3 1.3941073058907454 1.8946337069069605 4 0.7708821366256671 1.3968693073883145 5 0.5044297346176345 1.1425586035527182 6 0.33076778663953676 0.8990467162437907 7 0.2416553525597046 0.7663060610797006 8 0.1655789104018954 0.6000712350233655 9 0.17726701171369497 0.7227335817615137 >10 1.2191490741170132 10.964934444234258 >50 0.14605738165019608 4.736099571178577 >100 0.14284971090761564 13.567145929898489 >500 0.020057543688432797 6.3774063783753 >1k 0.012733845694076795 9.978927057995394 >5k 2.885426841561901E-4 0.8228131212075362 >10k+ 1.1526114516704483E-4 0.6102562734982725 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 14411 0.18569320868741665 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 11215 0.1445110912101435 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 10899 0.14043926732941184 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 10225 0.1317544277863323 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9640 0.12421639939953484 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9511 0.1225541674988564 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0023322788683937555 0.0 0.0 2 0.002589989240591961 0.0 0.0 3 0.0027832720197406144 0.0 0.0 4 0.0030152113547189992 0.0 0.0 5 0.003440433468846037 0.0 2.5771037219820505E-5 6 0.004046052843511819 0.0 2.5771037219820505E-5 7 0.00457435910651814 0.0 2.5771037219820505E-5 8 0.005373261260332576 0.0 2.5771037219820505E-5 9 0.005862910967509165 0.0 2.5771037219820505E-5 10-11 0.010012047959900267 0.0 3.8656555829730756E-5 12-13 0.013239870371682784 0.0 5.154207443964101E-5 14-15 0.01752430530947794 0.0 5.154207443964101E-5 16-17 0.022227519602095187 0.0 5.798483374459614E-5 18-19 0.027182001507605677 0.0 7.731311165946151E-5 20-21 0.03174347509551391 0.0 7.731311165946151E-5 22-23 0.03647246042535097 0.0 7.731311165946151E-5 24-25 0.04198101963108761 0.0 7.731311165946151E-5 26-27 0.04826270995341885 0.0 7.731311165946151E-5 28-29 0.05581362385882625 0.0 7.731311165946151E-5 30-31 0.103947478626146 0.0 1.0308414887928202E-4 32-33 0.11464890183167648 0.0 1.2885518609910253E-4 34-35 0.12307603100255778 0.0 1.2885518609910253E-4 36-37 0.13203790919575037 0.0 1.2885518609910253E-4 38-39 0.14185667437650196 0.0 1.2885518609910253E-4 40-41 0.1528351362321455 0.0 1.2885518609910253E-4 42-43 0.16513436374530485 0.0 1.5462622331892303E-4 44-45 0.17822605065297364 0.0 1.5462622331892303E-4 46-47 0.19206509764001728 0.0 1.6106898262387815E-4 48-49 0.20758570480565416 0.0 1.6751174192883327E-4 50-51 0.22500048320694788 0.0 1.6751174192883327E-4 52-53 0.24333013342954518 0.0 1.6751174192883327E-4 54-55 0.2628259230863394 0.0 1.6751174192883327E-4 56-57 0.28412568534852106 0.0 1.6751174192883327E-4 58-59 0.3078543678686708 0.0 1.6751174192883327E-4 60-61 0.3334063512721228 0.0 1.6751174192883327E-4 62-63 0.36170939289879067 0.0 1.8039726053874352E-4 64-65 0.3917777505750163 0.0 1.8039726053874352E-4 66-67 0.423985104340487 0.0 1.8039726053874352E-4 68-69 0.4596586626120235 0.0 1.9972553845360892E-4 70-71 0.49977772480397903 0.0 2.0616829775856404E-4 72-73 0.5433243349461707 0.0 2.0616829775856404E-4 74-75 0.5917481138822134 0.0 2.0616829775856404E-4 76-77 0.6454613982076243 0.0 2.0616829775856404E-4 78-79 0.7067127109198328 0.0 2.1261105706351917E-4 80-81 0.7747289209022441 0.0 2.190538163684743E-4 82-83 0.8484791866660654 0.0 2.3193933497838454E-4 84-85 0.9299543208365278 0.0 2.383820942833397E-4 86-87 1.0218667250810176 0.0 2.448248535882948E-4 88-89 1.1216586239554678 0.0 2.5771037219820506E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 4290 0.0 75.20951 2 GAGCGGC 2745 0.0 74.44472 9 CGGGAGA 3885 0.0 68.86966 4 AGAGCGG 3195 0.0 66.18637 8 GTTCGGA 790 0.0 63.66488 2 CGGAAAG 2000 0.0 63.56848 5 AAGCGGC 1100 0.0 59.05094 9 GGTTCGG 1120 0.0 56.353645 1 TCGGGAA 1640 0.0 53.516457 3 ATTCGGA 985 0.0 52.98801 2 TCGGAGA 1240 0.0 52.412968 4 TCGGGAG 5030 0.0 52.346115 3 GGATCGG 2060 0.0 50.911213 1 TCGGAAA 2035 0.0 50.819687 4 GATCGGG 6820 0.0 50.308613 1 GAGAGCG 5295 0.0 46.653854 7 TTGTGTT 63645 0.0 45.34184 1 CGGAGAG 2115 0.0 45.084034 5 GGTCCGG 540 0.0 44.81928 1 AAGGGGC 3900 0.0 43.036568 9 >>END_MODULE