FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n02_40255-1.342000000357f5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n02_40255-1.342000000357f5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7760650
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA144110.18569320868741665No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC112150.1445110912101435No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG108990.14043926732941184No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC102250.1317544277863323No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96400.12421639939953484No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA95110.1225541674988564No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA42900.075.209512
GAGCGGC27450.074.444729
CGGGAGA38850.068.869664
AGAGCGG31950.066.186378
GTTCGGA7900.063.664882
CGGAAAG20000.063.568485
AAGCGGC11000.059.050949
GGTTCGG11200.056.3536451
TCGGGAA16400.053.5164573
ATTCGGA9850.052.988012
TCGGAGA12400.052.4129684
TCGGGAG50300.052.3461153
GGATCGG20600.050.9112131
TCGGAAA20350.050.8196874
GATCGGG68200.050.3086131
GAGAGCG52950.046.6538547
TTGTGTT636450.045.341841
CGGAGAG21150.045.0840345
GGTCCGG5400.044.819281
AAGGGGC39000.043.0365689
Produced by FastQC (version 0.11.2)