FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n02_35011-1.342000000358a4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n02_35011-1.342000000358a4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6133638
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC137560.22427146825423996No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG129630.21134276264755109No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT98450.16050833127093578Illumina Single End PCR Primer 1 (97% over 34bp)
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA98370.1603779029672113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC26900.082.1372159
CGGGAGA39800.076.7633064
AGAGCGG31150.074.279968
GAGAGCG36700.072.737657
CGGAAAG22200.072.0146265
GATCGGG55600.066.829081
AAGCGGC14300.065.650119
TCGGGAG47150.063.9936563
TCGGGAA15550.062.848993
GTTCGGA8900.062.3755072
ATCGGGA57300.062.3531762
GATCCGG8800.060.396791
AGAGCGT73600.059.783328
GAGCGTC74400.058.5664449
AAGGGGC35200.055.899999
TCGGAAA21800.055.7101484
GGTTCGG12300.055.556631
AAGAGCG77550.054.3546757
GGATCGG23250.053.0675351
CGGAAGA83150.052.7752574
Produced by FastQC (version 0.11.2)