##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n02_35010-1.342000000357e8.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6960873 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16841939222279 33.0 33.0 33.0 33.0 33.0 2 32.11308265500606 33.0 33.0 33.0 33.0 33.0 3 32.089980667654764 33.0 33.0 33.0 33.0 33.0 4 35.86116497169249 37.0 37.0 37.0 33.0 37.0 5 35.86908294405026 37.0 37.0 37.0 33.0 37.0 6 35.91122104942871 37.0 37.0 37.0 33.0 37.0 7 35.8160786728906 37.0 37.0 37.0 33.0 37.0 8 35.81398554462925 37.0 37.0 37.0 33.0 37.0 9 35.742931382313685 37.0 37.0 37.0 33.0 37.0 10-11 35.68453346297225 37.0 37.0 37.0 33.0 37.0 12-13 35.688778117342466 37.0 37.0 37.0 33.0 37.0 14-15 37.53477782168989 40.0 37.0 40.0 33.0 40.0 16-17 37.509398533775865 40.0 37.0 40.0 33.0 40.0 18-19 37.477715079703366 40.0 37.0 40.0 33.0 40.0 20-21 37.39831002519368 40.0 37.0 40.0 33.0 40.0 22-23 37.50290021093618 40.0 37.0 40.0 33.0 40.0 24-25 37.285336408235 40.0 37.0 40.0 33.0 40.0 26-27 37.24790985268658 40.0 37.0 40.0 33.0 40.0 28-29 37.226858470194756 40.0 37.0 40.0 33.0 40.0 30-31 37.175695131918076 40.0 37.0 40.0 33.0 40.0 32-33 37.08635526032439 40.0 37.0 40.0 33.0 40.0 34-35 37.083467188670156 40.0 37.0 40.0 33.0 40.0 36-37 36.986990999548475 40.0 37.0 40.0 33.0 40.0 38-39 36.93894400889084 40.0 37.0 40.0 33.0 40.0 40-41 36.852870466103894 40.0 37.0 40.0 33.0 40.0 42-43 36.7877436493957 40.0 37.0 40.0 33.0 40.0 44-45 36.65815487511408 40.0 37.0 40.0 33.0 40.0 46-47 36.57162054242335 40.0 37.0 40.0 33.0 40.0 48-49 36.468138191861854 40.0 37.0 40.0 33.0 40.0 50-51 35.314633882847744 37.0 35.0 38.5 30.0 38.5 52-53 35.70887473453402 37.0 37.0 38.5 33.0 40.0 54-55 36.50712202334391 38.5 37.0 40.0 33.0 40.0 56-57 36.56151232754857 40.0 37.0 40.0 33.0 40.0 58-59 36.39477821244547 37.0 37.0 40.0 33.0 40.0 60-61 36.18185312388259 37.0 37.0 40.0 33.0 40.0 62-63 35.97043725693602 37.0 37.0 40.0 33.0 40.0 64-65 35.768047915828944 37.0 37.0 40.0 33.0 40.0 66-67 35.55204038344041 37.0 37.0 40.0 27.0 40.0 68-69 35.36580447596157 37.0 37.0 40.0 27.0 40.0 70-71 35.02527836666464 37.0 33.0 37.0 27.0 40.0 72-73 34.81301289364136 37.0 33.0 37.0 27.0 40.0 74-75 34.59231126612998 37.0 33.0 37.0 27.0 40.0 76-77 34.334035687765024 37.0 33.0 37.0 27.0 37.0 78-79 34.07417790843189 37.0 33.0 37.0 27.0 37.0 80-81 33.8121506454722 37.0 33.0 37.0 27.0 37.0 82-83 33.57543744872231 37.0 33.0 37.0 27.0 37.0 84-85 33.39279412223151 37.0 33.0 37.0 27.0 37.0 86-87 33.239926155814075 37.0 33.0 37.0 27.0 37.0 88-89 33.08782440076123 37.0 33.0 37.0 27.0 37.0 90-91 32.92817071939109 37.0 33.0 37.0 27.0 37.0 92-93 32.79413077928588 37.0 33.0 37.0 27.0 37.0 94-95 32.683347692164475 37.0 33.0 37.0 27.0 37.0 96-97 32.55393131005263 33.0 33.0 37.0 27.0 37.0 98-99 32.42380704259365 33.0 33.0 37.0 22.0 37.0 100-101 31.163500756298816 33.0 30.0 37.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.24969099033372544 2306 2 0.2301285264957187 2306 3 0.2652226852107873 2306 4 0.32553665862609193 2306 5 0.29182192258799233 2306 6 0.3405364846791983 2306 7 0.3452357650115161 2306 8 0.29122570512112844 2306 9 0.296630244579255 2306 10-11 0.3509976142982012 2306 12-13 0.32823204155412355 2306 14-15 0.5108833762493035 2306 16-17 0.5275089465071687 2306 18-19 0.5555273024565963 2306 20-21 0.6139226697617133 2306 22-23 0.5226955127283972 2306 24-25 0.5448525011782124 2306 26-27 0.5584673956861721 2306 28-29 0.4995899697896604 2306 30-31 0.49502059186699654 2306 32-33 0.4227927030606722 2306 34-35 0.42658572628561586 2306 36-37 0.47629170745045 2306 38-39 0.31562686366589787 2306 40-41 0.4427071569802479 2306 42-43 0.3759880811871241 2306 44-45 0.3669898679209638 2306 46-47 0.4297702610332763 2306 48-49 0.34667757445772907 2306 50-51 0.2501154999907058 2306 52-53 0.3146722777703346 2306 54-55 0.4958103464824717 2306 56-57 0.4980365223515122 2306 58-59 0.4797501600985328 2306 60-61 0.5940899548908476 2306 62-63 0.565415525834176 2306 64-65 0.4121098958956466 2306 66-67 0.4814009846680207 2306 68-69 0.4860078756866244 2306 70-71 0.4105306763222458 2306 72-73 0.4225911357375054 2306 74-75 0.371391810232943 2306 76-77 0.521238424641389 2306 78-79 0.5120139893826803 2306 80-81 0.5429679713351092 2306 82-83 0.3959583929044115 2306 84-85 0.43258518844997695 2306 86-87 0.5055811456546948 2306 88-89 0.5299924467400388 2306 90-91 0.3155341850149824 2306 92-93 0.3706892498911927 2306 94-95 0.35223367664699623 2306 96-97 0.3780883509422992 2306 98-99 0.37446492265480913 2306 100-101 0.27417417846199044 2307 1 0.19215226940890062 2307 2 0.1569628729504018 2307 3 0.10876354524786791 2307 4 0.17975879093732772 2307 5 0.21747884677392193 2307 6 0.21048696992808402 2307 7 0.2199326760281224 2307 8 0.20439585000883653 2307 9 0.14634485231431427 2307 10-11 0.21109944300117434 2307 12-13 0.19082569983634556 2307 14-15 0.37356858327116527 2307 16-17 0.3466794509186357 2307 18-19 0.4436784299079477 2307 20-21 0.46854004831786966 2307 22-23 0.3669548793679809 2307 24-25 0.31267128461761473 2307 26-27 0.3434597039693159 2307 28-29 0.2513778522163719 2307 30-31 0.27264034176540264 2307 32-33 0.2393355648192852 2307 34-35 0.22230877741984756 2307 36-37 0.24132891473338702 2307 38-39 0.1672118423523017 2307 40-41 0.15117589770592588 2307 42-43 0.14905387854192753 2307 44-45 0.2042826278986496 2307 46-47 0.22938158682490695 2307 48-49 0.1265417334371861 2307 50-51 0.03588606271208761 2307 52-53 0.05259467310727217 2307 54-55 0.12644933865480112 2307 56-57 0.22860249640305597 2307 58-59 0.2263481169794801 2307 60-61 0.2559904300968725 2307 62-63 0.2666275301773098 2307 64-65 0.22767100898408188 2307 66-67 0.1538903720460425 2307 68-69 0.1852458575142606 2307 70-71 0.06773171717416204 2307 72-73 0.06689484784732258 2307 74-75 0.10264149244928689 2307 76-77 0.19923890190286642 2307 78-79 0.28895384001264546 2307 80-81 0.2901017204827312 2307 82-83 0.11793246590467987 2307 84-85 0.05871325283104767 2307 86-87 0.16707817083902654 2307 88-89 0.26089047090998463 2307 90-91 0.24276880365475506 2307 92-93 0.17230552012613032 2307 94-95 0.16623555377930188 2307 96-97 0.22039747249630892 2307 98-99 0.21492715769535664 2307 100-101 0.11624428613520621 2201 1 0.2675341462923342 2201 2 0.26936616407554226 2201 3 0.23080679199355103 2201 4 0.35446729855189574 2201 5 0.31150930758702344 2201 6 0.26109124851258514 2201 7 0.3593817753746791 2201 8 0.25995760251254296 2201 9 0.2593837853495842 2201 10-11 0.28203518936266647 2201 12-13 0.3217247238868097 2201 14-15 0.5261580467926876 2201 16-17 0.48689477223631883 2201 18-19 0.5048942736727753 2201 20-21 0.5736877706602499 2201 22-23 0.5320243831117253 2201 24-25 0.5791811283111272 2201 26-27 0.5232351367203947 2201 28-29 0.4926551612468373 2201 30-31 0.41149402648979816 2201 32-33 0.4316875452137481 2201 34-35 0.4225530471568817 2201 36-37 0.4647139001582872 2201 38-39 0.430906664186665 2201 40-41 0.3755422005377085 2201 42-43 0.35284595396924345 2201 44-45 0.42292605968985697 2201 46-47 0.4154818405867573 2201 48-49 0.3521634575939814 2201 50-51 0.39502126738840815 2201 52-53 0.36260944045586285 2201 54-55 0.4938231426702515 2201 56-57 0.4799018568399447 2201 58-59 0.5088939916330304 2201 60-61 0.5228184754453338 2201 62-63 0.5512865429996694 2201 64-65 0.5376949131299398 2201 66-67 0.49480540706471743 2201 68-69 0.3779338690083591 2201 70-71 0.41125391191408767 2201 72-73 0.4689586600008653 2201 74-75 0.45439232488131154 2201 76-77 0.5364685716723585 2201 78-79 0.5118635216295147 2201 80-81 0.5472823865628627 2201 82-83 0.4999472261468796 2201 84-85 0.42622188805622585 2201 86-87 0.40754636571117686 2201 88-89 0.5477445358266735 2201 90-91 0.5652021916438983 2201 92-93 0.4871478158327349 2201 94-95 0.4561067921947668 2201 96-97 0.48751155035933635 2201 98-99 0.5873468214536075 2201 100-101 0.23104668449373023 1101 1 0.17499188328882553 1101 2 0.16940373335762615 1101 3 0.17306382223878813 1101 4 0.22170204067889898 1101 5 0.2250338101467264 1101 6 0.23068864561006563 1101 7 0.2821478410641518 1101 8 0.2529700462256912 1101 9 0.17415066688492686 1101 10-11 0.25897531185135847 1101 12-13 0.22497307290014845 1101 14-15 0.3859470885013465 1101 16-17 0.3241761819927689 1101 18-19 0.37811942713446456 1101 20-21 0.4359298092123467 1101 22-23 0.3149800969462788 1101 24-25 0.26242054594335684 1101 26-27 0.29366108413098146 1101 28-29 0.2286809960658971 1101 30-31 0.20784788132588972 1101 32-33 0.11136613555935071 1101 34-35 0.09518907615467498 1101 36-37 0.06958151787220856 1101 38-39 0.08375452870521372 1101 40-41 -0.01947591304909224 1101 42-43 0.03917998784793042 1101 44-45 0.0733239852861729 1101 46-47 0.05386151680956175 1101 48-49 0.14773427824209762 1101 50-51 0.11431466397080925 1101 52-53 0.1440191406246285 1101 54-55 0.24856448699919298 1101 56-57 0.176473529466179 1101 58-59 0.19553953950800462 1101 60-61 0.27007948792942216 1101 62-63 0.1306831404826454 1101 64-65 0.19593736335702516 1101 66-67 0.14676418568672034 1101 68-69 0.2702974818773356 1101 70-71 0.3225469949751414 1101 72-73 0.20204590759526297 1101 74-75 0.229054281073644 1101 76-77 0.2568609063381544 1101 78-79 0.30688892545052937 1101 80-81 0.18787168287065725 1101 82-83 0.10981379459986584 1101 84-85 0.21874579978052822 1101 86-87 0.2317271345536085 1101 88-89 0.2511207209540487 1101 90-91 0.22709394250939852 1101 92-93 0.24697528979509542 1101 94-95 0.15821887397385836 1101 96-97 0.17596721873779586 1101 98-99 0.2088187448781582 1101 100-101 0.0849930706180757 2304 1 0.24287684913534946 2304 2 0.1989119122361913 2304 3 0.2108948507023598 2304 4 0.2679110517387713 2304 5 0.28958630615128556 2304 6 0.2390228327708357 2304 7 0.30370322883078416 2304 8 0.26053654993467745 2304 9 0.26630504177845893 2304 10-11 0.3456919910482057 2304 12-13 0.2937913641804144 2304 14-15 0.4242451764969388 2304 16-17 0.4450302856299402 2304 18-19 0.42638212666891206 2304 20-21 0.5096648993689072 2304 22-23 0.4329863311228692 2304 24-25 0.41742446437150704 2304 26-27 0.4218401201306037 2304 28-29 0.2925616273304499 2304 30-31 0.20658915127108202 2304 32-33 0.15455011943900132 2304 34-35 0.09370566774121869 2304 36-37 0.2638247050500553 2304 38-39 0.2002290563955853 2304 40-41 0.23994082633733171 2304 42-43 0.17070320378562798 2304 44-45 0.24430455634172432 2304 46-47 0.24797956954400036 2304 48-49 0.1548436346995956 2304 50-51 0.166169010329277 2304 52-53 0.2715951471656979 2304 54-55 0.39283591607826907 2304 56-57 0.3321283867087601 2304 58-59 0.31167293574005583 2304 60-61 0.3597358385574836 2304 62-63 0.3535516981036224 2304 64-65 0.27799604813927203 2304 66-67 0.20651978577699026 2304 68-69 0.23596720527319803 2304 70-71 0.3804161196368696 2304 72-73 0.3358515880900086 2304 74-75 0.35222207619082013 2304 76-77 0.34159728356871 2304 78-79 0.3752388452792914 2304 80-81 0.35849254504017125 2304 82-83 0.23711672753029944 2304 84-85 0.22350179943936865 2304 86-87 0.3247385491924959 2304 88-89 0.45164426104923905 2304 90-91 0.23436537771076615 2304 92-93 0.2449770582625561 2304 94-95 0.3168716615905822 2304 96-97 0.2484642618170554 2304 98-99 0.1979895298112453 2304 100-101 0.10552642451885319 2202 1 0.1051637257218232 2202 2 0.0758419460597537 2202 3 0.05849741380039575 2202 4 0.0672573609869005 2202 5 0.11341830170895406 2202 6 0.0693595391730284 2202 7 0.15063310109293582 2202 8 0.0844381122739577 2202 9 -0.024425290063760485 2202 10-11 0.060931512194066784 2202 12-13 0.08633070691219302 2202 14-15 0.13935619996475168 2202 16-17 0.14229101340136907 2202 18-19 0.17083553422299502 2202 20-21 0.26915097455299275 2202 22-23 0.26142261004630285 2202 24-25 0.09091264482501771 2202 26-27 0.15192989480504338 2202 28-29 0.05149070583186699 2202 30-31 0.04578079567343707 2202 32-33 0.027001101664446026 2202 34-35 -0.07225651122448795 2202 36-37 0.04885180358028407 2202 38-39 0.08952729519992886 2202 40-41 -0.06351011088791836 2202 42-43 -0.06670475353856631 2202 44-45 0.018085087798986876 2202 46-47 0.0839848703766819 2202 48-49 0.019418845104425486 2202 50-51 0.00612060028861805 2202 52-53 -0.01700503074838622 2202 54-55 0.10691589452441264 2202 56-57 0.1125490053317364 2202 58-59 0.0535066351780884 2202 60-61 0.1835783832947513 2202 62-63 0.19517949489705444 2202 64-65 0.0076456619077944765 2202 66-67 0.11839932385702667 2202 68-69 0.14917640241213803 2202 70-71 0.19758535451817494 2202 72-73 0.2110518454784298 2202 74-75 0.1858183345789044 2202 76-77 0.19051138425823666 2202 78-79 0.24384259950976173 2202 80-81 0.18450132768015948 2202 82-83 0.18615299060973456 2202 84-85 0.10961691723165501 2202 86-87 0.10345598109327625 2202 88-89 0.09382727417065695 2202 90-91 0.11727442641262797 2202 92-93 0.139565118146173 2202 94-95 0.14187176925000955 2202 96-97 0.1645850614263722 2202 98-99 0.23592975538507233 2202 100-101 0.047687642746200964 1102 1 0.01729716819298943 1102 2 0.05904554697932696 1102 3 0.10249131514791543 1102 4 0.15555188508454876 1102 5 0.08581102995793799 1102 6 0.08466643210764602 1102 7 0.15572184113631948 1102 8 0.12662637823172673 1102 9 0.14614090965522308 1102 10-11 0.14979426990867495 1102 12-13 0.11975047811709771 1102 14-15 0.20277175207500875 1102 16-17 0.16555397599555732 1102 18-19 0.19479285994538031 1102 20-21 0.28340305516154984 1102 22-23 0.14467925057110875 1102 24-25 0.08105186103018269 1102 26-27 0.03902252464285283 1102 28-29 -0.027058673594702043 1102 30-31 0.02476240226106796 1102 32-33 0.0025824824174947025 1102 34-35 0.02389986235733943 1102 36-37 0.08549776844243695 1102 38-39 0.027820812787425098 1102 40-41 0.07463070068094169 1102 42-43 0.026287904762476444 1102 44-45 0.10819122706498518 1102 46-47 0.06494522952218773 1102 48-49 0.05314181943702323 1102 50-51 0.11501146657115413 1102 52-53 0.1639866423325813 1102 54-55 0.202524151804802 1102 56-57 0.29451982849697345 1102 58-59 0.2794566808808696 1102 60-61 0.36086188483041326 1102 62-63 0.3427288050664501 1102 64-65 0.2726995419038971 1102 66-67 0.3393720028929508 1102 68-69 0.22906244278136256 1102 70-71 0.2482700997395213 1102 72-73 0.26643940304893476 1102 74-75 0.31106188490439735 1102 76-77 0.2173793929917025 1102 78-79 0.15068151507382055 1102 80-81 0.13435469809603262 1102 82-83 0.28099593932825684 1102 84-85 0.15023668983871374 1102 86-87 0.2885479265041866 1102 88-89 0.32953684694305707 1102 90-91 0.26025750546919113 1102 92-93 0.16761361765605187 1102 94-95 0.3437365683630915 1102 96-97 0.29376621863880104 1102 98-99 0.12846069745305755 1102 100-101 0.22616623654353063 2305 1 0.07946087290712711 2305 2 0.13283274998307348 2305 3 0.1053004943340099 2305 4 0.14292378956744045 2305 5 0.128437276650061 2305 6 0.17998616845232362 2305 7 0.22020699396791343 2305 8 0.12653108556089165 2305 9 0.1426476192342534 2305 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17 13382.0 18 14405.0 19 15756.0 20 17619.0 21 20190.0 22 23002.0 23 24872.0 24 28032.0 25 32915.0 26 39089.0 27 47887.0 28 59532.0 29 73768.0 30 90772.0 31 117423.0 32 160097.0 33 231755.0 34 354723.0 35 599270.0 36 1186496.0 37 2445879.0 38 1200235.0 39 1027.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.375061570097216 26.74124265225123 21.51818401391261 16.36551176373895 2 31.90746064110947 24.48960852175913 30.000392916054565 13.602537921076838 3 36.13404545756054 21.2671500397631 25.3200364724095 17.27876803026686 4 30.901012891344383 20.87125920810313 30.244158609576427 17.98356929097606 5 29.848019173103097 19.566734717926355 32.61590068592734 17.969345423043205 6 26.454471813829677 19.786962156479497 35.46927895957111 18.289287070119705 7 32.11502955298468 19.860313285511257 27.959246248601737 20.065410912902326 8 29.79536201448826 27.400285554751726 23.667223022460007 19.13712940830001 9 27.56983925872912 26.976472843320003 25.563905882170236 19.889782015780636 10-11 23.364315742960702 29.46591986458132 29.94445421672725 17.225310175730723 12-13 23.60471502718452 27.45532631310122 30.141833747745167 18.79812491196909 14-15 22.378912242316083 27.695258446977718 31.184850786807534 18.740978523898665 16-17 22.07073065131494 27.976828900217544 32.06828760738285 17.884152841084667 18-19 21.842063980829014 28.119695566019814 31.936005079747055 18.102235373404113 20-21 21.751015212825344 28.858241888991415 31.20900641245697 18.181736485726272 22-23 22.287561424482817 28.775485344915484 30.4633723916032 18.4735808389985 24-25 21.661306442011078 29.11914563527141 30.48656937203471 18.732978550682805 26-27 21.47455185906146 29.483530886242747 30.369792363778398 18.672124890917395 28-29 21.66797827340578 29.35220531893333 30.116880918142936 18.86293548951796 30-31 21.870781664498487 29.10497054743213 30.17469795025795 18.849549837811434 32-33 21.30365054043521 29.44486182315775 30.366379283956746 18.885108352450285 34-35 22.041797441947296 28.992783400849802 30.230986621405247 18.73443253579765 36-37 21.43234287496927 28.774155646090843 30.76557913419401 19.02792234474588 38-39 21.243940323189968 29.238640615653438 30.55794223261164 18.959476828544954 40-41 21.36540150701305 29.67434242393358 29.770757262714593 19.189498806338776 42-43 21.503205104882323 29.062134430613963 30.206307845833635 19.228352618670076 44-45 21.09759662996477 29.125487987471566 30.466523909990546 19.310391472573123 46-47 21.37501386821664 29.34915198556841 29.901604102398586 19.37423004381636 48-49 21.022841784372414 29.571061608437216 29.76189121336742 19.64420539382295 50-51 20.957529066565346 29.228544355225356 29.89763350282631 19.91629307538299 52-53 21.68445502749715 29.099532717383532 29.362383241481655 19.853629013637665 54-55 21.79475434149242 29.267826990817607 29.23017321027144 19.70724545741853 56-57 21.783067962093817 29.205877340124253 29.37042782261021 19.640626875171716 58-59 21.57661925122697 29.265976033725565 29.504761483572732 19.652643231474734 60-61 20.860159846533502 29.330009858091017 29.408997002247418 20.400833293128066 62-63 21.02910667879025 29.195870359079052 29.504141824026988 20.270881138103718 64-65 20.73200832292056 29.3339172261386 29.758069939548086 20.17600451139275 66-67 20.61390635445918 29.071209311958146 29.708466995472886 20.60641733810979 68-69 20.459285722772982 29.119138261363954 30.358276421909437 20.06329959395363 70-71 20.307599966532976 29.994179272362963 29.66285717659572 20.03536358450834 72-73 20.301765181817803 30.394932235270606 29.202521239879353 20.100781343032242 74-75 20.429228239379817 30.37437496048839 29.134347547529497 20.062049252602293 76-77 20.45400558794497 30.326723339120775 29.139398439427794 20.079872633506458 78-79 20.319519313319557 30.256493487556956 29.416900403008423 20.007086796115065 80-81 20.16659009504003 30.568007846311385 29.248364271631004 20.017037787017582 82-83 20.074869316156796 30.40077290961314 29.279361042946817 20.244996731283248 84-85 20.242908593698004 30.009756562739753 29.46131183633982 20.28602300722242 86-87 20.35483704380155 29.992580672135933 29.556294519298355 20.096287764764163 88-89 20.57197932659686 30.28108052591439 29.10995308158207 20.03698706590668 90-91 20.617218209952252 30.11415104517228 29.07300455685234 20.195626188023127 92-93 20.383667059907342 30.23799280934763 29.039269947544312 20.339070183200718 94-95 20.14679904428343 30.28901534337649 29.016826558754357 20.54735905358572 96-97 20.238997495206725 30.573388225534927 28.86711794846984 20.320496330788508 98-99 20.53065351906737 30.491545729166635 28.717710112373148 20.26009063939285 100-101 20.639718052069707 30.321436974567806 28.397953002484606 20.640891970877885 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 215.0 1 270.5 2 340.5 3 335.0 4 307.0 5 367.5 6 552.0 7 885.0 8 1332.5 9 1793.5 10 2196.5 11 2407.5 12 2427.5 13 2336.0 14 2090.5 15 1783.5 16 1587.0 17 1515.0 18 1516.5 19 1650.0 20 1948.5 21 2433.5 22 3286.5 23 4870.5 24 7292.0 25 11113.5 26 17951.5 27 27328.0 28 38804.0 29 52691.5 30 69274.5 31 88595.5 32 113538.0 33 148132.0 34 190814.5 35 238695.0 36 293404.5 37 353003.5 38 408860.5 39 442735.0 40 458233.5 41 452654.0 42 426153.5 43 403693.5 44 375068.5 45 346820.0 46 331086.0 47 303848.5 48 262143.0 49 222477.0 50 182884.0 51 147344.5 52 119140.0 53 95043.0 54 77815.0 55 61750.0 56 44362.0 57 30568.0 58 21817.0 59 16047.0 60 11700.0 61 8333.0 62 5906.5 63 4042.0 64 2801.5 65 2024.5 66 1442.0 67 1063.0 68 804.5 69 607.0 70 461.0 71 356.0 72 295.5 73 240.0 74 195.0 75 158.5 76 130.0 77 116.5 78 103.5 79 87.5 80 70.0 81 55.5 82 50.5 83 48.0 84 44.5 85 37.0 86 32.0 87 26.0 88 23.0 89 21.5 90 15.0 91 11.5 92 12.0 93 10.5 94 7.5 95 6.0 96 4.0 97 4.0 98 3.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19404175309619928 2 0.1842297654331576 3 0.15748024708969693 4 0.15045526617135524 5 0.15400367166589593 6 0.1355002454433517 7 0.10916734151018127 8 0.09610863464970558 9 0.09559145814037981 10-11 0.0755580514110802 12-13 0.0953831509352347 14-15 0.10028196175968157 16-17 0.09243093502783344 18-19 0.08901182366062417 20-21 0.08692156860209918 22-23 0.10653836092110859 24-25 0.45609221716873727 26-27 0.2877799954114951 28-29 0.12699556506777238 30-31 0.2368524752570547 32-33 0.2691688240828413 34-35 0.2514842606667296 36-37 0.25462323475805404 38-39 0.2726741315349382 40-41 0.2728896217471573 42-43 0.30065912709512155 44-45 0.2761794389870351 46-47 0.2951210286410914 48-49 0.27783871362112195 50-51 0.29403639457292213 52-53 0.29344738799285663 54-55 0.26828531421274315 56-57 0.28505045272338686 58-59 0.2740532688931403 60-61 0.24907795329694996 62-63 0.24179438412394536 64-65 0.23956765193101498 66-67 0.2495520317638319 68-69 0.2542856334255775 70-71 0.240257220610116 72-73 0.23037340287633462 74-75 0.24008482844034076 76-77 0.2416004429329482 78-79 0.24384154114002657 80-81 0.251161025348401 82-83 0.2774220992108317 84-85 0.2712159810989225 86-87 0.2710795039645171 88-89 0.26888150379988257 90-91 0.26062822867189217 92-93 0.23618445559917559 94-95 0.23481968425512145 96-97 0.22969101720430757 98-99 0.2550685811966401 100-101 0.26310636611241145 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6960873.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.21134300634017 #Duplication Level Percentage of deduplicated Percentage of total 1 91.82523401822115 60.798720662178965 2 4.527721772881583 5.995730786830743 3 1.2154739818887115 2.414344941903467 4 0.5620288724521268 1.4885074581357751 5 0.3429483649974681 1.1353535914155453 6 0.1998641280346381 0.7939963401578707 7 0.1635020868271571 0.757798492721572 8 0.14545781715434644 0.7704765939647955 9 0.09856938197580895 0.5873770043930925 >10 0.7627198785757695 10.168671262567399 >50 0.09013519061208101 4.1265761194790045 >100 0.06047754759174554 7.822149615184451 >500 0.0050127731392381955 2.2504534502704803 >1k 8.324041222964318E-4 0.7740507206910098 >5k 2.1781525921937405E-5 0.11579296010587646 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT 8029 0.11534472759379463 Illumina Single End PCR Primer 1 (97% over 34bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0010343530186515397 0.0 0.0 2 0.001120549103539168 0.0 0.0 3 0.0012498432308706107 0.0 0.0 4 0.001321673301610301 0.0 0.0 5 0.0015084314855334955 0.0 0.0 6 0.0017957517684922568 0.0 0.0 7 0.002183634150486584 0.0 0.0 8 0.002470954433445345 0.0 1.4366014147938053E-5 9 0.0027726407305520445 0.0 1.4366014147938053E-5 10-11 0.004611490541488115 0.0 2.1549021221907078E-5 12-13 0.0066011835009775355 0.0 2.8732028295876105E-5 14-15 0.008842281708055871 0.0 2.8732028295876105E-5 16-17 0.011148026978799928 0.0 2.8732028295876105E-5 18-19 0.013180817980733164 0.0 2.8732028295876105E-5 20-21 0.015034033805817172 0.0 2.8732028295876105E-5 22-23 0.016858517602605302 0.0 2.8732028295876105E-5 24-25 0.018841027555020755 0.0 2.8732028295876105E-5 26-27 0.02118987086820863 0.0 2.8732028295876105E-5 28-29 0.024235465867571495 0.0 2.8732028295876105E-5 30-31 0.04214988551005025 0.0 2.8732028295876105E-5 32-33 0.04716362444768063 0.0 5.0281049517783186E-5 34-35 0.05112146134543756 0.0 7.183007073969026E-5 36-37 0.055811964964739336 0.0 7.90130778136593E-5 38-39 0.06031571040011792 0.0 8.619608488762833E-5 40-41 0.06545156045800576 0.0 1.077451061095354E-4 42-43 0.07165049556284103 0.0 1.1492811318350442E-4 44-45 0.07850308431140748 0.0 1.1492811318350442E-4 46-47 0.08593031362589146 0.0 1.1492811318350442E-4 48-49 0.09428415085291744 0.0 1.1492811318350442E-4 50-51 0.10345685088637589 0.0 1.2929412733144247E-4 52-53 0.11311081239379026 0.0 1.4366014147938053E-4 54-55 0.12364828377130282 0.0 1.4366014147938053E-4 56-57 0.13468138263691926 0.0 1.4366014147938053E-4 58-59 0.14741685417906633 0.0 1.5084314855334956E-4 60-61 0.16026007082732296 0.0 1.580261556273186E-4 62-63 0.1759477582768713 0.0 1.580261556273186E-4 64-65 0.1925548706318877 0.0 1.580261556273186E-4 66-67 0.21135998315153862 0.0 1.580261556273186E-4 68-69 0.2335770240313248 0.0 1.580261556273186E-4 70-71 0.2574317905239759 0.0 1.580261556273186E-4 72-73 0.28502890370216494 0.0 1.7239216977525665E-4 74-75 0.3141919124224792 0.0 1.8675818392319468E-4 76-77 0.34875654246241816 0.0 2.0112419807113275E-4 78-79 0.38764534276088647 0.0 2.0112419807113275E-4 80-81 0.43091577737447584 0.0 2.0112419807113275E-4 82-83 0.4801696568806815 0.0 2.0112419807113275E-4 84-85 0.5342074190981505 0.0 2.154902122190708E-4 86-87 0.5976908356178887 0.0 2.370392334409779E-4 88-89 0.6671145989877993 0.0 2.6577126173685397E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 1480 0.0 56.703716 9 CGGGAGA 2155 0.0 54.379333 4 GATCGGG 2715 0.0 53.83931 1 AGAGCGG 1745 0.0 47.008415 8 GAGAGCG 2250 0.0 44.887794 7 ATCGGGA 3285 0.0 41.17033 2 TTGTGTT 55760 0.0 39.62861 1 CGCCGGA 800 0.0 37.69921 58-59 GGTTCGG 655 0.0 36.952812 1 CGGAAAG 1240 0.0 35.965855 5 TCGGGAG 3190 0.0 35.846092 3 TCGGGAA 1020 0.0 35.81839 3 TGTGTTT 98965 0.0 35.199223 1 GATCCGG 675 0.0 35.154816 1 GGCGCCG 1485 0.0 35.025906 56-57 GTTCGGA 560 0.0 34.743267 2 CGCCGTA 1575 0.0 34.67894 58-59 AGCGGCG 1435 0.0 34.526943 10-11 GTCGCCG 1625 0.0 33.616013 56-57 GCGTCGG 600 0.0 33.58392 10-11 >>END_MODULE