FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n02_35010-1.342000000357e8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n02_35010-1.342000000357e8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6960873
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT80290.11534472759379463Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC14800.056.7037169
CGGGAGA21550.054.3793334
GATCGGG27150.053.839311
AGAGCGG17450.047.0084158
GAGAGCG22500.044.8877947
ATCGGGA32850.041.170332
TTGTGTT557600.039.628611
CGCCGGA8000.037.6992158-59
GGTTCGG6550.036.9528121
CGGAAAG12400.035.9658555
TCGGGAG31900.035.8460923
TCGGGAA10200.035.818393
TGTGTTT989650.035.1992231
GATCCGG6750.035.1548161
GGCGCCG14850.035.02590656-57
GTTCGGA5600.034.7432672
CGCCGTA15750.034.6789458-59
AGCGGCG14350.034.52694310-11
GTCGCCG16250.033.61601356-57
GCGTCGG6000.033.5839210-11
Produced by FastQC (version 0.11.2)