FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n02_35008-2.342000000358b0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n02_35008-2.342000000358b0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5888638
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA38550.085.195042
GAGCGGC26950.081.8175359
GATCGGG43850.075.5587841
AGAGCGG32600.071.42068
AAGCGGC10850.068.1784449
TCGGGAG41800.066.180313
CGGGAGA42650.064.301814
GATCCGG8650.063.1074871
GTTCGGA7450.062.4313052
GAGAGCG40650.062.294217
GGATCGG19350.058.8747941
GGTTCGG9700.056.2762531
TCGGAAA19000.055.438794
TCGGGAA15750.055.4336933
TCGGAGA11450.054.285044
GAGCGTC85000.052.4955989
TTCGGAA10600.051.9264533
CGGAAAG27800.050.177135
AGAGCGT89850.049.715578
AAAGCGG16650.048.9862068