##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n02_35008-1.342000000357b1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2434739 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.853232317714546 33.0 33.0 33.0 27.0 33.0 2 31.779497925650347 33.0 33.0 33.0 27.0 33.0 3 31.73474569553451 33.0 33.0 33.0 27.0 33.0 4 35.44425665338256 37.0 37.0 37.0 33.0 37.0 5 35.447789680947324 37.0 37.0 37.0 33.0 37.0 6 35.49618788707948 37.0 37.0 37.0 33.0 37.0 7 35.39063119291226 37.0 37.0 37.0 33.0 37.0 8 35.38576701650567 37.0 37.0 37.0 33.0 37.0 9 35.289534114334224 37.0 37.0 37.0 33.0 37.0 10-11 35.22233224998655 37.0 37.0 37.0 33.0 37.0 12-13 35.209024252702235 37.0 37.0 37.0 33.0 37.0 14-15 36.90311836299497 40.0 37.0 40.0 33.0 40.0 16-17 36.84989397220811 40.0 37.0 40.0 33.0 40.0 18-19 36.848923436967986 40.0 37.0 40.0 33.0 40.0 20-21 36.775259689026214 40.0 37.0 40.0 33.0 40.0 22-23 36.89740933217072 40.0 37.0 40.0 33.0 40.0 24-25 36.64136073722892 40.0 37.0 40.0 33.0 40.0 26-27 36.56492318067768 40.0 37.0 40.0 33.0 40.0 28-29 36.56310984462811 40.0 37.0 40.0 33.0 40.0 30-31 36.5324944480702 40.0 37.0 40.0 33.0 40.0 32-33 36.44092508478322 40.0 37.0 40.0 33.0 40.0 34-35 36.39746888680881 40.0 37.0 40.0 33.0 40.0 36-37 36.308935372538905 40.0 37.0 40.0 33.0 40.0 38-39 36.2558091442245 40.0 37.0 40.0 33.0 40.0 40-41 36.179422517156866 40.0 37.0 40.0 33.0 40.0 42-43 36.05226145389712 38.5 37.0 40.0 33.0 40.0 44-45 35.89993485954757 37.0 37.0 40.0 27.0 40.0 46-47 35.84087719463976 37.0 37.0 40.0 27.0 40.0 48-49 35.719594995603224 37.0 37.0 40.0 27.0 40.0 50-51 34.52797445639963 37.0 35.0 38.5 27.0 38.5 52-53 34.949608151017415 37.0 35.0 38.5 27.0 40.0 54-55 35.75492424444674 37.0 37.0 40.0 27.0 40.0 56-57 35.807023463295245 37.0 37.0 40.0 27.0 40.0 58-59 35.59344923624257 37.0 37.0 40.0 27.0 40.0 60-61 35.317761985987005 37.0 37.0 40.0 27.0 40.0 62-63 35.098886574700614 37.0 37.0 40.0 27.0 40.0 64-65 34.85299266163642 37.0 33.0 40.0 27.0 40.0 66-67 34.616395432939626 37.0 33.0 37.0 27.0 40.0 68-69 34.418443619624114 37.0 33.0 37.0 27.0 40.0 70-71 34.05883135728306 37.0 33.0 37.0 27.0 40.0 72-73 33.83572304875389 37.0 33.0 37.0 27.0 38.5 74-75 33.608249795974025 37.0 33.0 37.0 27.0 37.0 76-77 33.373468983739116 37.0 33.0 37.0 27.0 37.0 78-79 33.15087592550988 37.0 33.0 37.0 27.0 37.0 80-81 32.945248340787245 37.0 33.0 37.0 27.0 37.0 82-83 32.761078292170126 37.0 33.0 37.0 24.5 37.0 84-85 32.59015771300332 37.0 33.0 37.0 22.0 37.0 86-87 32.45300112250224 37.0 33.0 37.0 22.0 37.0 88-89 32.32858963527507 35.0 33.0 37.0 22.0 37.0 90-91 32.17816201243747 33.0 33.0 37.0 22.0 37.0 92-93 32.0178125868933 33.0 33.0 37.0 22.0 37.0 94-95 31.908445422692125 33.0 33.0 37.0 22.0 37.0 96-97 31.778976925247427 33.0 33.0 37.0 22.0 37.0 98-99 31.639848049421314 33.0 33.0 37.0 22.0 37.0 100-101 30.395330669940392 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.16665926292646915 2306 2 0.21063729309989654 2306 3 0.26223101907030966 2306 4 0.31478557074896685 2306 5 0.21916188011898186 2306 6 0.19089962973615826 2306 7 0.3959960921030188 2306 8 0.24240084466320866 2306 9 0.2519689940829011 2306 10-11 0.3430650563849653 2306 12-13 0.2165277019727867 2306 14-15 0.49319825477472534 2306 16-17 0.45798422805042804 2306 18-19 0.4351890866354253 2306 20-21 0.5179324270392556 2306 22-23 0.3607708150483049 2306 24-25 0.2868548534009818 2306 26-27 0.31849440947854646 2306 28-29 0.4366174361784587 2306 30-31 0.3581705070202261 2306 32-33 0.3527845876449831 2306 34-35 0.34340145925894916 2306 36-37 0.37026660085784613 2306 38-39 0.26391019424503526 2306 40-41 0.13859060504468346 2306 42-43 0.21099028577818046 2306 44-45 0.1790864883984966 2306 46-47 0.3156823179946642 2306 48-49 0.1823866202178479 2306 50-51 0.24535989655574042 2306 52-53 0.19612794762270624 2306 54-55 0.3935322467614881 2306 56-57 0.21825837640194834 2306 58-59 0.25410866090025763 2306 60-61 0.4645535600427735 2306 62-63 0.30787525884590394 2306 64-65 0.21823654814875937 2306 66-67 0.19615984385670515 2306 68-69 0.14141619180271192 2306 70-71 0.2826317254956763 2306 72-73 0.18424508794198857 2306 74-75 0.04625035316387738 2306 76-77 0.09178727796839325 2306 78-79 0.3046989470707899 2306 80-81 0.12059230043698932 2306 82-83 0.1363216634493014 2306 84-85 0.0510913185064652 2306 86-87 0.049158022062073314 2306 88-89 0.3442777488751858 2306 90-91 0.09474055150460714 2306 92-93 0.1597672370343517 2306 94-95 0.0662614935693675 2306 96-97 0.14493768031110577 2306 98-99 -0.0640154456522275 2306 100-101 -0.23108409918789974 2307 1 0.24971139437931456 2307 2 0.10986896091892717 2307 3 0.1520220198201514 2307 4 0.25741597792010396 2307 5 0.1888342400845815 2307 6 0.23405972066506564 2307 7 0.24533150033649775 2307 8 0.1909150653259175 2307 9 0.1262715468218616 2307 10-11 0.21002255629746713 2307 12-13 0.2869047361681325 2307 14-15 0.37363622147913134 2307 16-17 0.2921354236762639 2307 18-19 0.396547056790034 2307 20-21 0.40648664473677343 2307 22-23 0.24951876235495263 2307 24-25 0.32875556408276196 2307 26-27 0.27953546775478344 2307 28-29 0.24596421465899 2307 30-31 0.09308474377654363 2307 32-33 0.07752939779960855 2307 34-35 0.02453767928287931 2307 36-37 0.037448639725568 2307 38-39 0.041727823308768563 2307 40-41 -0.03352699185939656 2307 42-43 -0.09753706881506474 2307 44-45 0.07108870733279815 2307 46-47 0.11555012440505408 2307 48-49 0.09663026592799895 2307 50-51 -0.018504929229528955 2307 52-53 -0.04029368737251815 2307 54-55 0.06516524406718105 2307 56-57 0.11221442596034592 2307 58-59 0.12728038938448094 2307 60-61 0.17711352183697926 2307 62-63 0.19629375558681517 2307 64-65 0.17858673853012164 2307 66-67 -0.07039570156379682 2307 68-69 0.07823939548418224 2307 70-71 -0.0876388814236364 2307 72-73 -0.05136607937122761 2307 74-75 -0.043663884970321476 2307 76-77 0.04288102353334722 2307 78-79 -0.05369476716122534 2307 80-81 0.05862886324933925 2307 82-83 -0.07614308699361771 2307 84-85 -0.1277818751302746 2307 86-87 0.06708269569106307 2307 88-89 0.08854694870576196 2307 90-91 0.17423500265319802 2307 92-93 0.24090632304395854 2307 94-95 -0.02626148047500365 2307 96-97 -0.041657553499589994 2307 98-99 -0.053294179018831045 2307 100-101 -0.11257944926391872 2201 1 0.3373350608504957 2201 2 0.36833455439279206 2201 3 0.35847804549243634 2201 4 0.3701051759248344 2201 5 0.3797582709493099 2201 6 0.32049307881845124 2201 7 0.31497244420144455 2201 8 0.3405375588847832 2201 9 0.3549441131935396 2201 10-11 0.3434381692100459 2201 12-13 0.3799249853790556 2201 14-15 0.6165152599546104 2201 16-17 0.593344709451749 2201 18-19 0.585222390707635 2201 20-21 0.6281616689527993 2201 22-23 0.5647659635094087 2201 24-25 0.5439790434151206 2201 26-27 0.5452309454529711 2201 28-29 0.4291798394324786 2201 30-31 0.4656426810323282 2201 32-33 0.40201229502481084 2201 34-35 0.4013095346588429 2201 36-37 0.46263172606641945 2201 38-39 0.3607636630283295 2201 40-41 0.4088301665298957 2201 42-43 0.37966797339000635 2201 44-45 0.3973162127855261 2201 46-47 0.38444752093507617 2201 48-49 0.3413191476417694 2201 50-51 0.3056597002158412 2201 52-53 0.46731034270147376 2201 54-55 0.46732656563624886 2201 56-57 0.6140638055688044 2201 58-59 0.6496499358364218 2201 60-61 0.5674994627037435 2201 62-63 0.5307085843613848 2201 64-65 0.4837051170117945 2201 66-67 0.521704536313365 2201 68-69 0.5003847707172824 2201 70-71 0.5237088475413643 2201 72-73 0.5198561875124525 2201 74-75 0.4763394097271956 2201 76-77 0.5418093243260529 2201 78-79 0.4759522389471442 2201 80-81 0.43662502822143523 2201 82-83 0.5024695817772553 2201 84-85 0.464795039583187 2201 86-87 0.6777381428144622 2201 88-89 0.6442735942424349 2201 90-91 0.6040278979674767 2201 92-93 0.42499925987142717 2201 94-95 0.3595154421457316 2201 96-97 0.5606235632710757 2201 98-99 0.5606240729464567 2201 100-101 0.28381588808015934 1101 1 0.3604080296815688 1101 2 0.3308652346066161 1101 3 0.3582048498027497 1101 4 0.41267253965882844 1101 5 0.4086844332980206 1101 6 0.3806979755857185 1101 7 0.5439468743398521 1101 8 0.4575912930016628 1101 9 0.40278014751194036 1101 10-11 0.4748185073259137 1101 12-13 0.45832949359061104 1101 14-15 0.5654718310108251 1101 16-17 0.561304791989663 1101 18-19 0.5792049660355545 1101 20-21 0.677410863484063 1101 22-23 0.5168945730350671 1101 24-25 0.536954269972064 1101 26-27 0.5711667975604655 1101 28-29 0.5343791250691865 1101 30-31 0.5247872551294179 1101 32-33 0.4378294083696517 1101 34-35 0.46647551552066346 1101 36-37 0.5915599397961842 1101 38-39 0.5538995006554828 1101 40-41 0.22271355154998673 1101 42-43 0.28725579059988604 1101 44-45 0.38301700075719225 1101 46-47 0.5490388737605514 1101 48-49 0.5399856292819152 1101 50-51 0.5152251694390912 1101 52-53 0.6541655755332272 1101 54-55 0.7560837907210214 1101 56-57 0.5441866470935821 1101 58-59 0.5809429092529399 1101 60-61 0.5895757016775747 1101 62-63 0.4825766684921362 1101 64-65 0.49553989636766005 1101 66-67 0.5048076725130031 1101 68-69 0.4769919071099835 1101 70-71 0.5783735674071835 1101 72-73 0.45961460722501357 1101 74-75 0.41330264173839026 1101 76-77 0.3222772805471692 1101 78-79 0.5065111799518363 1101 80-81 0.4876883676284649 1101 82-83 0.4937340771978498 1101 84-85 0.40741103311530225 1101 86-87 0.5284248727923782 1101 88-89 0.5314038328059851 1101 90-91 0.5493572652974237 1101 92-93 0.4547721599208998 1101 94-95 0.47375588814010783 1101 96-97 0.40928268407328616 1101 98-99 0.3936744765858471 1101 100-101 0.2567661360559299 2304 1 0.219711162859209 2304 2 0.12340405566673951 2304 3 0.14967149409051217 2304 4 0.21115706827475833 2304 5 0.16958966869392356 2304 6 0.1960846957169835 2304 7 0.2588372373807388 2304 8 0.22738449357390067 2304 9 0.08805736399592234 2304 10-11 0.18439650326886436 2304 12-13 0.12776008598904554 2304 14-15 0.327474812824903 2304 16-17 0.19809911893758425 2304 18-19 0.283888778788814 2304 20-21 0.41239732905529536 2304 22-23 0.28390188420541307 2304 24-25 0.29739258615187936 2304 26-27 0.2640280365562262 2304 28-29 0.10561255233272959 2304 30-31 0.15443530316013465 2304 32-33 0.1997377606398203 2304 34-35 0.10450734776280513 2304 36-37 0.2996246279404815 2304 38-39 0.17995785452102098 2304 40-41 0.28338846344397695 2304 42-43 0.217683516138365 2304 44-45 0.25417516147355457 2304 46-47 0.2810859227830278 2304 48-49 0.14961089628802426 2304 50-51 0.06573723984172375 2304 52-53 0.19804133939907587 2304 54-55 0.4127957790385324 2304 56-57 0.3139793013058707 2304 58-59 0.31239562791821385 2304 60-61 0.4105027302792976 2304 62-63 0.2762436135838371 2304 64-65 0.19850868522751597 2304 66-67 0.16501680161960763 2304 68-69 0.2896583010337608 2304 70-71 0.3127980471304497 2304 72-73 0.2655173927428933 2304 74-75 0.3184337921670206 2304 76-77 0.15662159833809142 2304 78-79 0.11105923318390865 2304 80-81 0.2509041592668879 2304 82-83 0.04203754212525723 2304 84-85 0.18025084605913833 2304 86-87 0.3435832144631661 2304 88-89 0.37420110397841455 2304 90-91 0.29171128556694015 2304 92-93 0.2587686955617059 2304 94-95 0.24036315455265012 2304 96-97 0.205700551529862 2304 98-99 0.40724059422632664 2304 100-101 0.33954076170102 2202 1 0.09161711070272105 2202 2 0.14373967344704752 2202 3 0.12986487424389992 2202 4 0.049534161593712156 2202 5 0.022674682035017213 2202 6 0.05261375020639747 2202 7 0.24725482863237858 2202 8 0.1398059467730306 2202 9 -0.0743950938549176 2202 10-11 0.18773137168592768 2202 12-13 0.19922348535116896 2202 14-15 0.29021681574560887 2202 16-17 0.0817465384820224 2202 18-19 0.17143440208331384 2202 20-21 0.32762020907436806 2202 22-23 0.2468186038930682 2202 24-25 0.06079719988643717 2202 26-27 0.12629880916790626 2202 28-29 0.16072559307102097 2202 30-31 0.20490047392809885 2202 32-33 0.06087863083030953 2202 34-35 -0.10860459013902357 2202 36-37 -0.007876275941057997 2202 38-39 0.07557044382350853 2202 40-41 -0.16562251846424658 2202 42-43 -0.02544940797395867 2202 44-45 -0.005559491223849022 2202 46-47 0.06907641738296633 2202 48-49 0.05090036662353725 2202 50-51 -0.00668346601401737 2202 52-53 0.06226601102245155 2202 54-55 0.22266030789911184 2202 56-57 0.13047377601435528 2202 58-59 0.2107546032094234 2202 60-61 0.31562526429856774 2202 62-63 0.3002608065216421 2202 64-65 0.22457279113213247 2202 66-67 0.3152984692860983 2202 68-69 0.29448391754156944 2202 70-71 0.28383041113919916 2202 72-73 0.33111253056715384 2202 74-75 0.2028263332759579 2202 76-77 0.25507146674505066 2202 78-79 0.2742375230602363 2202 80-81 0.3042913417216866 2202 82-83 0.3099496464624778 2202 84-85 0.22936817291280676 2202 86-87 0.27311984135904055 2202 88-89 0.2927685476802324 2202 90-91 0.34847221849388177 2202 92-93 0.17015790594291502 2202 94-95 0.3747532718451616 2202 96-97 0.2061954140489739 2202 98-99 0.10781695757256315 2202 100-101 -0.06902914621415945 1102 1 -0.08498731779890178 1102 2 0.014992793000118354 1102 3 0.026227377075599634 1102 4 0.09357803847035484 1102 5 -0.07610426919605118 1102 6 0.06816386806138297 1102 7 -0.07497155403404321 1102 8 -0.013064790929888659 1102 9 0.009835740902843781 1102 10-11 0.06997439820938212 1102 12-13 0.15036412313478564 1102 14-15 0.0563768963250908 1102 16-17 0.11262276547503092 1102 18-19 0.0831472590416169 1102 20-21 0.23775115073474495 1102 22-23 0.06715827711929734 1102 24-25 0.02341386134214929 1102 26-27 -0.03559902113963176 1102 28-29 -0.07264739969667033 1102 30-31 0.08943638365811779 1102 32-33 -0.10741332376509405 1102 34-35 -0.03368785622917159 1102 36-37 0.12813829726289327 1102 38-39 -0.0023579108310585184 1102 40-41 0.026712320741864914 1102 42-43 -0.027481961785660758 1102 44-45 0.11396783446966197 1102 46-47 -0.01658805444136391 1102 48-49 0.06542938665795361 1102 50-51 0.014134741504364001 1102 52-53 0.06898964249113959 1102 54-55 0.20568750534693692 1102 56-57 0.22556767619988705 1102 58-59 0.21990207826704022 1102 60-61 0.4034619560464776 1102 62-63 0.174727369967691 1102 64-65 0.20190606705133263 1102 66-67 0.3261974809517767 1102 68-69 0.24564140512421062 1102 70-71 0.33471305977783317 1102 72-73 0.4347417900559307 1102 74-75 0.352448336117952 1102 76-77 0.2836660437308467 1102 78-79 0.05081976599314686 1102 80-81 0.19944459064376474 1102 82-83 0.18207202842495462 1102 84-85 0.23397556323465807 1102 86-87 0.24381710476446727 1102 88-89 0.23686016565410029 1102 90-91 0.3645145377002166 1102 92-93 0.273277084571383 1102 94-95 0.23713646997446958 1102 96-97 0.24671543629071024 1102 98-99 0.11390020306852122 1102 100-101 0.04914869779789299 2305 1 0.19049197432936538 2305 2 0.16938253790765856 2305 3 0.1751255518060546 2305 4 0.24391746643916434 2305 5 0.29344558018500777 2305 6 0.2612755029388083 2305 7 0.280892631776652 2305 8 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-0.9976675529530894 1213 96-97 -0.9759484128820617 1213 98-99 -0.7963581782636879 1213 100-101 -0.6426479136303307 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 238.0 3 97.0 4 137.0 5 134.0 6 501.0 7 2509.0 8 8944.0 9 15439.0 10 14996.0 11 13631.0 12 10124.0 13 7475.0 14 6096.0 15 5395.0 16 5412.0 17 5565.0 18 6183.0 19 6775.0 20 7479.0 21 8483.0 22 9748.0 23 10516.0 24 12038.0 25 14157.0 26 16738.0 27 19995.0 28 23954.0 29 29235.0 30 36772.0 31 47919.0 32 64999.0 33 93078.0 34 141692.0 35 239059.0 36 475017.0 37 846367.0 38 227686.0 39 156.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 34.021058054703865 24.9518237434155 26.332526657940143 14.694591543940492 2 29.372337628512852 22.785559078982505 35.05350356052649 12.788599731978165 3 38.29015565354745 19.792269974990127 26.058477030406742 15.85909734105568 4 28.102598400452784 18.67046069658126 36.559657253984476 16.667283648981485 5 31.76205350789225 17.63908621465515 34.23914429492933 16.35971598252327 6 24.325496963276372 18.532486033703353 41.11832364959565 16.023693353424623 7 32.23045387958025 18.729229272648855 31.858816409280617 17.18150043849028 8 27.537975752906735 27.716225721355343 27.903396410088387 16.84240211564953 9 30.63754216263214 25.691734376959115 25.423990100902554 18.246733359506194 10-11 24.803922515562384 28.564006904146904 28.77621139562369 17.855859184667022 12-13 25.59602968894845 25.480944932385906 28.863108953343296 20.059916425322346 14-15 24.162724470830355 26.06776397226985 30.12473468031933 19.64477687658046 16-17 23.048473383172315 27.582118806199368 31.115778205846006 18.253629604782308 18-19 22.111490979016466 27.930437449156642 31.214666564174955 18.74340500765194 20-21 22.106693620752235 28.519010563639362 30.599141968917028 18.775153846691374 22-23 22.77311294050027 27.794732724216093 30.546203268314322 18.88595106696932 24-25 22.490191479402778 28.048191558205133 30.55674687384672 18.90487008854538 26-27 21.98162416150506 28.534508963030024 30.428129770143443 19.055737105321473 28-29 21.43719115167872 28.543676264760414 30.183732430870307 19.83540015269056 30-31 21.579443220967136 28.669095690517032 29.946587136410596 19.804873952105236 32-33 20.698052566436953 28.501108904033007 31.521433629349932 19.279404900180104 34-35 20.514324429875153 29.271167852932052 31.164052545795577 19.050455171397214 36-37 20.89288684804754 30.074820021839493 30.685288154464168 18.347004975648805 38-39 21.880966304535832 29.11628682738796 29.836320432250783 19.16642643582542 40-41 21.467066823846757 28.580571099110973 29.46406049717642 20.488301579865848 42-43 21.840305376026052 28.692564090470096 30.361541372021218 19.10558916148263 44-45 20.97955722595034 29.264567100676906 30.91562632494409 18.84024934842866 46-47 21.232438559932053 29.187734353342332 30.632811177369774 18.947015909355844 48-49 21.216720783232265 29.78103795078605 29.78400324205178 19.2182380239299 50-51 20.78760101066271 29.46341702523444 29.84361007927622 19.905371884826632 52-53 21.29317856831719 29.032610179866037 29.77678471209757 19.897426539719206 54-55 21.932977957398954 29.13062752630882 29.336222298498075 19.60017221779415 56-57 22.202319551271422 28.986091630039045 28.77774512531578 20.033843693373747 58-59 21.75223021470147 28.663707941588484 29.77142808899897 19.81263375471108 60-61 20.599863838554604 29.121791616168828 29.88103475287233 20.397309792404233 62-63 21.31264403070871 29.16975527516491 29.52293221048991 19.994668483636467 64-65 20.992466586262495 29.20801892647465 29.55378155879107 20.24573292847178 66-67 21.094929085175814 28.838420676540576 29.36696993297096 20.699680305312647 68-69 21.197628608227895 28.702540871014605 30.032434110621033 20.067396410136475 70-71 20.69298849616453 30.235089116900255 29.56237668396822 19.509545702966996 72-73 20.33854243852317 31.24415447339215 28.884414179436263 19.532888908648417 74-75 21.081517591947026 30.514603639037425 28.609945834987844 19.7939329340277 76-77 21.440003491226957 29.70025469900416 29.189436321234872 19.670305488534016 78-79 21.411811834725878 29.52433749463746 29.75160217482418 19.312248495812483 80-81 20.3969114697076 30.35374895830245 29.51555299728912 19.733786574700833 82-83 19.600097277085872 30.191644507849812 29.754062358236254 20.454195856828065 84-85 19.690317511239673 29.551084349952166 30.242061189451242 20.51653694935692 86-87 19.648603843951413 29.74189505927401 31.02853837722662 19.580962719547955 88-89 19.981151314136802 30.219710528331508 30.406632497160548 19.392505660371143 90-91 20.39352291271275 29.597623882076952 29.87884697502449 20.130006230185806 92-93 19.745784726170328 30.56357069725489 29.70771274592099 19.98293183065379 94-95 19.31257183695112 31.088438902775994 29.79154522311034 19.807444037162554 96-97 19.87362711663465 31.516756581638 29.22457830938448 19.38503799234287 98-99 20.408714942327588 30.46093665556625 28.819941632739194 20.310406769366967 100-101 20.327451189516818 30.204766632501833 28.412760050452494 21.05502212752885 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 133.0 1 135.0 2 145.5 3 177.0 4 198.5 5 200.5 6 262.0 7 394.0 8 530.5 9 695.5 10 834.0 11 937.0 12 977.0 13 924.5 14 828.0 15 758.0 16 703.0 17 679.5 18 735.0 19 801.0 20 950.5 21 1152.0 22 1424.0 23 1878.0 24 2543.5 25 3408.0 26 4754.0 27 6623.5 28 8540.0 29 10431.0 30 12747.0 31 15620.5 32 21109.5 33 31326.5 34 47329.5 35 70652.5 36 103004.5 37 142967.5 38 183437.0 39 202998.5 40 198894.5 41 177138.0 42 150853.5 43 141705.0 44 135029.0 45 125925.5 46 124350.5 47 111315.5 48 83126.5 49 62543.5 50 50368.0 51 41726.0 52 34964.5 53 27946.5 54 21337.5 55 15751.0 56 11526.0 57 8258.5 58 6194.0 59 4899.5 60 3852.0 61 2908.0 62 2205.0 63 1670.5 64 1322.5 65 1025.0 66 786.5 67 649.0 68 518.5 69 403.0 70 327.0 71 266.5 72 211.0 73 182.0 74 155.5 75 119.5 76 80.0 77 50.0 78 41.5 79 35.5 80 29.5 81 27.0 82 24.5 83 22.5 84 19.0 85 14.0 86 10.5 87 8.5 88 6.5 89 6.5 90 5.5 91 5.0 92 6.0 93 5.0 94 3.5 95 2.5 96 1.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18893195533484286 2 0.17825319264200393 3 0.1519259353877356 4 0.14584725508565805 5 0.14872230657988392 6 0.1302398326884319 7 0.10391257543416357 8 0.09035876124709877 9 0.0901123282618794 10-11 0.07017179254121284 12-13 0.08970160661984714 14-15 0.09430168901060852 16-17 0.08645690564779224 18-19 0.08304791601892442 20-21 0.08050144183832436 22-23 0.10169467856718933 24-25 0.44988395060004377 26-27 0.2824532732255901 28-29 0.12418168846845595 30-31 0.23174968651670674 32-33 0.2645252735508816 34-35 0.2480142635411845 36-37 0.25099199544591844 38-39 0.26774943844083493 40-41 0.26805747967235916 42-43 0.29467224207605003 44-45 0.2698646548973011 46-47 0.2894766133043419 48-49 0.27300667545884794 50-51 0.28853195352766764 52-53 0.288141767967737 54-55 0.2636216859384107 56-57 0.27980411863448196 58-59 0.26916642810584623 60-61 0.24661780995827476 62-63 0.23766407816197135 64-65 0.23659620189268749 66-67 0.2470696037645103 68-69 0.2514848614163572 70-71 0.23684263487790685 72-73 0.22717014020804693 74-75 0.2370890678631262 76-77 0.23795158331139393 78-79 0.24039537708148592 80-81 0.24688477902559575 82-83 0.2720414796000721 84-85 0.26737978896300585 86-87 0.26625030444741715 88-89 0.265038675603422 90-91 0.2562903046281347 92-93 0.23230416073345025 94-95 0.2311130679715567 96-97 0.22587636703564531 98-99 0.2526554180961491 100-101 0.2594117891075799 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2434739.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.38990458234712 #Duplication Level Percentage of deduplicated Percentage of total 1 88.2757106212474 38.30274660794811 2 5.335414358661417 4.630062398592073 3 1.7021294011393375 2.215656969067305 4 0.9908970333888804 1.719797109186974 5 0.6065615582816776 1.3159324068580887 6 0.4542246698651467 1.1825259050638102 7 0.3210680070728288 0.9751777133934075 8 0.22701335922892674 0.7880070396688978 9 0.21028082063603523 0.8211658268605697 >10 1.472980882612152 12.479977442768105 >50 0.19200518745228345 5.84115153171429 >100 0.1786782306267269 17.019809653368554 >500 0.026196749365256976 7.66678067302131 >1k 0.006743373979075603 4.80640039399013 >5k 9.574644298209064E-5 0.2348083284982028 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 5652 0.2321398720766374 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 4755 0.19529814078634303 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 4238 0.17406383189327482 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 3836 0.15755282188357767 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 3685 0.15135092508889045 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 3511 0.144204368517529 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 2997 0.12309327611707045 No Hit TTGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC 2993 0.12292898746025754 No Hit TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA 2761 0.11340024536510894 No Hit GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT 2728 0.11204486394640248 No Hit TTGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGG 2649 0.10880016297434755 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 8.625154482677609E-4 0.0 0.0 2 9.035876124709876E-4 0.0 0.0 3 9.446597766742143E-4 0.0 0.0 4 9.857319408774412E-4 0.0 0.0 5 0.0012321649260968012 0.0 0.0 6 0.0012321649260968012 0.0 0.0 7 0.001437525747112935 0.0 0.0 8 0.0017661030607387487 0.0 0.0 9 0.001848247389145202 0.0 0.0 10-11 0.0030598762331403902 0.0 0.0 12-13 0.004004536009814604 0.0 0.0 14-15 0.0052161648538097925 0.0 0.0 16-17 0.00642779369780498 0.0 2.0536082101613354E-5 18-19 0.008296577169051796 0.0 4.107216420322671E-5 20-21 0.010226968886603452 0.0 4.107216420322671E-5 22-23 0.01248593791778092 0.0 4.107216420322671E-5 24-25 0.015278845083600338 0.0 4.107216420322671E-5 26-27 0.0182976491525375 0.0 4.107216420322671E-5 28-29 0.021398597549881118 0.0 4.107216420322671E-5 30-31 0.035014019983250766 0.0 4.107216420322671E-5 32-33 0.039531958045605706 0.0 4.107216420322671E-5 34-35 0.043166844577591276 0.0 4.107216420322671E-5 36-37 0.04694548368428813 0.0 4.107216420322671E-5 38-39 0.05224379286650438 0.0 4.107216420322671E-5 40-41 0.05787067936234644 0.0 4.107216420322671E-5 42-43 0.06327166895507075 0.0 4.107216420322671E-5 44-45 0.07002803996650155 0.0 4.107216420322671E-5 46-47 0.07659958623901782 0.0 4.107216420322671E-5 48-49 0.08368453456407443 0.0 4.107216420322671E-5 50-51 0.09095430762804554 0.0 4.107216420322671E-5 52-53 0.0977928229678828 0.0 4.107216420322671E-5 54-55 0.10684923517469429 0.0 4.107216420322671E-5 56-57 0.11559760614998157 0.0 4.107216420322671E-5 58-59 0.1256397502976705 0.0 4.107216420322671E-5 60-61 0.13736585317769173 0.0 4.107216420322671E-5 62-63 0.1490098117293065 0.0 4.107216420322671E-5 64-65 0.16061269811671808 0.0 4.107216420322671E-5 66-67 0.17233880099673926 0.0 4.107216420322671E-5 68-69 0.18743282134142508 0.0 4.107216420322671E-5 70-71 0.2043134808289513 0.0 4.107216420322671E-5 72-73 0.2223441609141678 0.0 4.107216420322671E-5 74-75 0.24226416055273275 0.0 4.107216420322671E-5 76-77 0.2626564900796348 0.0 4.107216420322671E-5 78-79 0.28610869583967724 0.0 4.107216420322671E-5 80-81 0.31128593249625525 0.0 4.107216420322671E-5 82-83 0.3399543031101075 0.0 4.107216420322671E-5 84-85 0.3737772303314647 0.0 4.107216420322671E-5 86-87 0.4100439513229139 0.0 4.107216420322671E-5 88-89 0.4489598269054712 0.0 4.107216420322671E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CACGCGG 20 0.0020832089 71.17354 1 ATTCGGA 90 0.0 63.25886 2 GAGCGGC 570 0.0 62.38416 9 CGGGAGA 755 0.0 60.943447 4 CGGAAAG 330 0.0 56.05882 5 AGAGCGG 635 0.0 54.50621 8 GTGCGCT 35 2.8073802E-4 54.21184 4 TTGTGTT 30985 0.0 51.56071 1 GGATCGG 505 0.0 50.73757 1 GATCCGG 180 0.0 50.085083 1 GAGAGCG 835 0.0 49.969154 7 TCGGGAG 960 0.0 47.43634 3 TCGGGAA 240 0.0 47.43634 3 TCGGAAA 290 0.0 45.799664 4 GGTACGG 65 2.609786E-6 43.799095 1 CGACGGG 100 6.4392225E-10 42.70412 1 CGGAGAG 435 0.0 42.52738 5 AAGGGGC 690 0.0 41.227795 9 AAGCGGC 185 0.0 41.004936 9 GTTGACG 70 4.3441178E-6 40.670593 1 >>END_MODULE