FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n02_35008-1.342000000357b1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n02_35008-1.342000000357b1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2434739
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC56520.2321398720766374No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG47550.19529814078634303No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC42380.17406383189327482No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA38360.15755282188357767No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG36850.15135092508889045No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA35110.144204368517529No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA29970.12309327611707045No Hit
TTGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC29930.12292898746025754No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA27610.11340024536510894No Hit
GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT27280.11204486394640248No Hit
TTGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGG26490.10880016297434755No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCGG200.002083208971.173541
ATTCGGA900.063.258862
GAGCGGC5700.062.384169
CGGGAGA7550.060.9434474
CGGAAAG3300.056.058825
AGAGCGG6350.054.506218
GTGCGCT352.8073802E-454.211844
TTGTGTT309850.051.560711
GGATCGG5050.050.737571
GATCCGG1800.050.0850831
GAGAGCG8350.049.9691547
TCGGGAG9600.047.436343
TCGGGAA2400.047.436343
TCGGAAA2900.045.7996644
GGTACGG652.609786E-643.7990951
CGACGGG1006.4392225E-1042.704121
CGGAGAG4350.042.527385
AAGGGGC6900.041.2277959
AAGCGGC1850.041.0049369
GTTGACG704.3441178E-640.6705931
Produced by FastQC (version 0.11.2)