FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n02_35005-1.3420000003578b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n02_35005-1.3420000003578b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2665757
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG62930.23606802870629243No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC61110.22924069973369662No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA29400.1102876218650087No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA28620.10736162373389621No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA28020.10511085594073277No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA4900.063.909762
CGGACGG407.589604E-659.3202251
CGTTGCG250.005043755856.937782
CGGGAGA5050.055.4261444
GAGCGGC4050.053.85539
CGGAAAG2850.051.6025165
GATCCGG2300.045.3928641
AGAGCGG4500.045.30878
CGCGAGG751.3736462E-744.2924351
GTTGCGC459.6711906E-442.183271
GGTACGG704.339776E-640.6767231
CGAGGCG951.945773E-839.9630971
ATCCGGA2150.039.7240332
GGTCCGG1208.731149E-1139.5468141
GAGAGCG6000.039.5126577
TTGTGTT328300.038.9276541
TCGGAAA2700.038.6556434
AAGCGGC2250.035.825489
GTGACGC801.084981E-535.5921361
GTTGACG1203.8326107E-935.5921331
Produced by FastQC (version 0.11.2)