##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n02_35005-1.3420000003578b.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2665757 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.08646924682182 33.0 33.0 33.0 33.0 33.0 2 32.035102224246245 33.0 33.0 33.0 33.0 33.0 3 32.01822559220514 33.0 33.0 33.0 33.0 33.0 4 35.7820514022846 37.0 37.0 37.0 33.0 37.0 5 35.79111261829191 37.0 37.0 37.0 33.0 37.0 6 35.81421562430484 37.0 37.0 37.0 33.0 37.0 7 35.73989377126272 37.0 37.0 37.0 33.0 37.0 8 35.70667731529918 37.0 37.0 37.0 33.0 37.0 9 35.64917657535927 37.0 37.0 37.0 33.0 37.0 10-11 35.58052046754449 37.0 37.0 37.0 33.0 37.0 12-13 35.575114685997264 37.0 37.0 37.0 33.0 37.0 14-15 37.34963445655399 40.0 37.0 40.0 33.0 40.0 16-17 37.326054100204935 40.0 37.0 40.0 33.0 40.0 18-19 37.29916999186348 40.0 37.0 40.0 33.0 40.0 20-21 37.20842616187447 40.0 37.0 40.0 33.0 40.0 22-23 37.27846386598628 40.0 37.0 40.0 33.0 40.0 24-25 37.08064857374472 40.0 37.0 40.0 33.0 40.0 26-27 37.018686249346814 40.0 37.0 40.0 33.0 40.0 28-29 37.02087549615363 40.0 37.0 40.0 33.0 40.0 30-31 36.985081535938946 40.0 37.0 40.0 33.0 40.0 32-33 36.88876555515 40.0 37.0 40.0 33.0 40.0 34-35 36.85792159600444 40.0 37.0 40.0 33.0 40.0 36-37 36.767427788804454 40.0 37.0 40.0 33.0 40.0 38-39 36.719248416115946 40.0 37.0 40.0 33.0 40.0 40-41 36.616386827456516 40.0 37.0 40.0 33.0 40.0 42-43 36.527708639609685 40.0 37.0 40.0 33.0 40.0 44-45 36.39274866388797 40.0 37.0 40.0 33.0 40.0 46-47 36.311298254116934 40.0 37.0 40.0 33.0 40.0 48-49 36.187350534951236 37.0 37.0 40.0 33.0 40.0 50-51 34.97892587358862 37.0 35.0 38.5 27.0 38.5 52-53 35.39042530883347 37.0 37.0 38.5 30.0 40.0 54-55 36.1980782569454 37.0 37.0 40.0 33.0 40.0 56-57 36.23795792339662 37.0 37.0 40.0 33.0 40.0 58-59 36.04442940598112 37.0 37.0 40.0 33.0 40.0 60-61 35.778184583215946 37.0 37.0 40.0 27.0 40.0 62-63 35.53556982125528 37.0 37.0 40.0 27.0 40.0 64-65 35.28823913807598 37.0 37.0 40.0 27.0 40.0 66-67 35.04782225086532 37.0 35.0 37.0 27.0 40.0 68-69 34.828841113424815 37.0 33.0 37.0 27.0 40.0 70-71 34.478344800370024 37.0 33.0 37.0 27.0 40.0 72-73 34.25357262496169 37.0 33.0 37.0 27.0 40.0 74-75 34.01996412276138 37.0 33.0 37.0 27.0 37.0 76-77 33.78645596729184 37.0 33.0 37.0 27.0 37.0 78-79 33.56665067371107 37.0 33.0 37.0 27.0 37.0 80-81 33.35576160917893 37.0 33.0 37.0 27.0 37.0 82-83 33.164501115443 37.0 33.0 37.0 27.0 37.0 84-85 33.00828263791486 37.0 33.0 37.0 27.0 37.0 86-87 32.87355580422371 37.0 33.0 37.0 27.0 37.0 88-89 32.74322659567245 37.0 33.0 37.0 27.0 37.0 90-91 32.59670480092521 37.0 33.0 37.0 24.5 37.0 92-93 32.47277996456541 33.0 33.0 37.0 22.0 37.0 94-95 32.362638079915016 33.0 33.0 37.0 22.0 37.0 96-97 32.22032465824904 33.0 33.0 37.0 22.0 37.0 98-99 32.082922224343775 33.0 33.0 37.0 22.0 37.0 100-101 30.80907168207755 33.0 30.0 37.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.2840735893210109 2306 2 0.21222990631105176 2306 3 0.24003623185904388 2306 4 0.32832796142622556 2306 5 0.3252496960856064 2306 6 0.25151614822130597 2306 7 0.3339094745633133 2306 8 0.29193331121756216 2306 9 0.38946156004317345 2306 10-11 0.3082337831620521 2306 12-13 0.38244606131389247 2306 14-15 0.5490337236551497 2306 16-17 0.5796897895595734 2306 18-19 0.6133561490184221 2306 20-21 0.5711788995467728 2306 22-23 0.5365433628189464 2306 24-25 0.4122102332506117 2306 26-27 0.5497949384179677 2306 28-29 0.47726181184801675 2306 30-31 0.45512169520343804 2306 32-33 0.40209235045245606 2306 34-35 0.4067714586454869 2306 36-37 0.40989152650021055 2306 38-39 0.31309109738263174 2306 40-41 0.1641055695695286 2306 42-43 0.3086245311224829 2306 44-45 0.3881819856526718 2306 46-47 0.18314113221026673 2306 48-49 0.2283556254772563 2306 50-51 0.23296901853093743 2306 52-53 0.33667947650047125 2306 54-55 0.5460745594300676 2306 56-57 0.47212398254446697 2306 58-59 0.5613753580917944 2306 60-61 0.543149008646509 2306 62-63 0.5417641221199503 2306 64-65 0.5525140955857424 2306 66-67 0.38936963890898113 2306 68-69 0.2982058128398464 2306 70-71 0.28472182043825 2306 72-73 0.30300868294364847 2306 74-75 0.4446036218463547 2306 76-77 0.41112160507709206 2306 78-79 0.43885642037388806 2306 80-81 0.45193978277205815 2306 82-83 0.3891509910355566 2306 84-85 0.31146652963766286 2306 86-87 0.40438924449158975 2306 88-89 0.4112092924301862 2306 90-91 0.4721615538263606 2306 92-93 0.3344953829067592 2306 94-95 0.3550345295069306 2306 96-97 0.24150389600277578 2306 98-99 0.28850635405805036 2306 100-101 -0.06392872995690624 2307 1 0.3849688468830834 2307 2 0.3600450482252313 2307 3 0.35999116674898346 2307 4 0.48103321644955344 2307 5 0.44051259604311355 2307 6 0.42674744866498315 2307 7 0.5487512956550091 2307 8 0.5119767768230972 2307 9 0.4298239408126605 2307 10-11 0.47084689928515644 2307 12-13 0.5193276133540508 2307 14-15 0.8174236084629243 2307 16-17 0.7430410491228869 2307 18-19 0.7103735380504688 2307 20-21 0.8476028073037227 2307 22-23 0.671659415241777 2307 24-25 0.7205777342944728 2307 26-27 0.7259708299789978 2307 28-29 0.6676835230760787 2307 30-31 0.6762387817245425 2307 32-33 0.6143814739146762 2307 34-35 0.6634403230809696 2307 36-37 0.6346402787471845 2307 38-39 0.4860370592381713 2307 40-41 0.4963640420613089 2307 42-43 0.5260915855140027 2307 44-45 0.6483344221537379 2307 46-47 0.5877927610545441 2307 48-49 0.5640683767831263 2307 50-51 0.281827139689689 2307 52-53 0.38205356062139373 2307 54-55 0.5546221442247372 2307 56-57 0.5601832162887987 2307 58-59 0.6674155335537222 2307 60-61 0.7504710660928993 2307 62-63 0.5379332157867509 2307 64-65 0.49944007107426813 2307 66-67 0.42497256377039605 2307 68-69 0.5173725732759138 2307 70-71 0.3274560246336051 2307 72-73 0.45592317547759365 2307 74-75 0.5274368000339464 2307 76-77 0.5112324544604974 2307 78-79 0.4594302798202179 2307 80-81 0.522948949234916 2307 82-83 0.5332582117755535 2307 84-85 0.3976905179277992 2307 86-87 0.4262754881017088 2307 88-89 0.6074847717468259 2307 90-91 0.6105828614585036 2307 92-93 0.5083580375449017 2307 94-95 0.46397194703248346 2307 96-97 0.4363407511908832 2307 98-99 0.5162406930953551 2307 100-101 0.3148530055613037 2201 1 0.19615513951375618 2201 2 0.1351087840630143 2201 3 0.1985871072148626 2201 4 0.25555527023281854 2201 5 0.2192348069275738 2201 6 0.22462323333363798 2201 7 0.18576872191948723 2201 8 0.21717603141857467 2201 9 0.20437100217635162 2201 10-11 0.1601479195497788 2201 12-13 0.11818135071462166 2201 14-15 0.3185029534965409 2201 16-17 0.3338392196397564 2201 18-19 0.21528429001502047 2201 20-21 0.34602409214854646 2201 22-23 0.32456011594524625 2201 24-25 0.07992762205062576 2201 26-27 0.18085469879459026 2201 28-29 0.13327744641266293 2201 30-31 0.009563369847462866 2201 32-33 -0.009839327837283918 2201 34-35 -0.008867488081428121 2201 36-37 -0.011205716534419707 2201 38-39 -0.04201586394702872 2201 40-41 -0.10185177871651518 2201 42-43 -0.010268431651915932 2201 44-45 -0.0200498782659011 2201 46-47 -0.08954012244004872 2201 48-49 -0.05224467078799222 2201 50-51 -0.0932122137571838 2201 52-53 0.06455129096018908 2201 54-55 0.0819884753581448 2201 56-57 0.253520169767107 2201 58-59 0.057435432442716206 2201 60-61 0.01568382478180297 2201 62-63 0.0542959882521572 2201 64-65 0.22269234263336557 2201 66-67 0.1941894379925415 2201 68-69 0.126900081837654 2201 70-71 0.14321044304882946 2201 72-73 0.3317943658964779 2201 74-75 0.2070560908814869 2201 76-77 0.1366233653660771 2201 78-79 0.032193072648937004 2201 80-81 -0.06968660859713083 2201 82-83 -0.051344442332549534 2201 84-85 0.07818317619341286 2201 86-87 -0.01226481049773298 2201 88-89 0.1661115831776101 2201 90-91 0.050167338181587695 2201 92-93 0.13875883760885444 2201 94-95 0.21906366903213836 2201 96-97 0.19282206851877248 2201 98-99 0.01952785022913872 2201 100-101 0.022098081189135144 1101 1 0.165005900333405 1101 2 0.1940560378843017 1101 3 0.1260636695166184 1101 4 0.1706726572272217 1101 5 0.20760579583349426 1101 6 0.2163659110226206 1101 7 0.2810434871332248 1101 8 0.2678465218973827 1101 9 0.16754314002677262 1101 10-11 0.16972590182447078 1101 12-13 0.23627354295081204 1101 14-15 0.3760020551162384 1101 16-17 0.3152758995863536 1101 18-19 0.4048205334183663 1101 20-21 0.3964157573076861 1101 22-23 0.3176898600685263 1101 24-25 0.27222571977639376 1101 26-27 0.34780721683791427 1101 28-29 0.2149963896718603 1101 30-31 0.25662930891476066 1101 32-33 0.06210834836599588 1101 34-35 -0.021025704516183907 1101 36-37 0.17325089231120927 1101 38-39 0.09409888070423023 1101 40-41 -0.03845171012036275 1101 42-43 0.054453524762521965 1101 44-45 0.1945507661122079 1101 46-47 0.01334590891280385 1101 48-49 0.07947445664940034 1101 50-51 0.12282440659753036 1101 52-53 0.16397466996698284 1101 54-55 0.2925903042066693 1101 56-57 0.34305972837793064 1101 58-59 0.3918300755585875 1101 60-61 0.3274269035699575 1101 62-63 0.28224208216011704 1101 64-65 0.36791654070105295 1101 66-67 0.28453088799061277 1101 68-69 0.26700924220610744 1101 70-71 0.313916308019202 1101 72-73 0.2757979536876718 1101 74-75 0.2359785133895329 1101 76-77 0.2210931122561277 1101 78-79 0.22659172266370575 1101 80-81 0.1812304964818523 1101 82-83 0.22030652280211172 1101 84-85 0.2055924315978075 1101 86-87 0.29546471850542133 1101 88-89 0.16311612951398047 1101 90-91 0.2715871801485079 1101 92-93 0.2799403243443379 1101 94-95 0.13348537406380245 1101 96-97 0.2702449531094402 1101 98-99 0.33435652767418844 1101 100-101 0.2648586503570556 2304 1 0.24193109229707943 2304 2 0.189831452917133 2304 3 0.25365657260292807 2304 4 0.31072982547964756 2304 5 0.3169096838881629 2304 6 0.2960000671268901 2304 7 0.22920057600400412 2304 8 0.23453410012870535 2304 9 0.1938150232871223 2304 10-11 0.2990640227464354 2304 12-13 0.3483539181768549 2304 14-15 0.47615152230154223 2304 16-17 0.48815187941847427 2304 18-19 0.5350349573851858 2304 20-21 0.5902387467112433 2304 22-23 0.5399854036981324 2304 24-25 0.4914384910188403 2304 26-27 0.48600600538870253 2304 28-29 0.4015656099435816 2304 30-31 0.3704015943205903 2304 32-33 0.2960308528756386 2304 34-35 0.22403463975027194 2304 36-37 0.3720657228377604 2304 38-39 0.22636331136724408 2304 40-41 0.3801224354046724 2304 42-43 0.21902276897592543 2304 44-45 0.30154194360163444 2304 46-47 0.35036027616776977 2304 48-49 0.2430636816945011 2304 50-51 0.1396487704033973 2304 52-53 0.2775434510584418 2304 54-55 0.3218352583097186 2304 56-57 0.2605708070181336 2304 58-59 0.2380701276018229 2304 60-61 0.27987179841733223 2304 62-63 0.3038881702011693 2304 64-65 0.28509598960837224 2304 66-67 0.20299299391610504 2304 68-69 0.24003734525411602 2304 70-71 0.38691422209998194 2304 72-73 0.33786667604719156 2304 74-75 0.34626803194471023 2304 76-77 0.43357022737298223 2304 78-79 0.35885250596079743 2304 80-81 0.45635495582999397 2304 82-83 0.2565041045423868 2304 84-85 0.27793718661079225 2304 86-87 0.3272092405358862 2304 88-89 0.4682122104847508 2304 90-91 0.13908267798964147 2304 92-93 0.3945198934089902 2304 94-95 0.46857346449753123 2304 96-97 0.3016060341064062 2304 98-99 0.20169546934701543 2304 100-101 0.21404074257593209 2202 1 0.13859803157098582 2202 2 0.18170810002637694 2202 3 0.18573604978681146 2202 4 0.19999985015317634 2202 5 0.1704062660963075 2202 6 0.18978268462618075 2202 7 0.2618926993863866 2202 8 0.27648646861414505 2202 9 0.10124309055149894 2202 10-11 0.18339008363866327 2202 12-13 0.209917874493172 2202 14-15 0.3533465035165051 2202 16-17 0.2448806919636226 2202 18-19 0.23644738865258574 2202 20-21 0.4780562377062054 2202 22-23 0.3199912741836357 2202 24-25 0.16962212782918584 2202 26-27 0.301275422782453 2202 28-29 0.2295941606657621 2202 30-31 0.07086461514828812 2202 32-33 0.1170638560113062 2202 34-35 -0.05743285132184184 2202 36-37 0.042983262233356356 2202 38-39 0.054049302801210786 2202 40-41 -0.06796611634278094 2202 42-43 -0.03481297778874648 2202 44-45 -0.007904223069054694 2202 46-47 -0.0013133522706212375 2202 48-49 -0.05267282677595375 2202 50-51 -0.10330103197350127 2202 52-53 -0.004905083180432257 2202 54-55 0.2161232279121208 2202 56-57 0.11926029999781207 2202 58-59 -0.040381281529008106 2202 60-61 0.1954509079243536 2202 62-63 0.16944359373938056 2202 64-65 0.11268279658376201 2202 66-67 0.14679852585427255 2202 68-69 0.10902041908764204 2202 70-71 0.15249659775035695 2202 72-73 0.18581887273862918 2202 74-75 0.16733643973154244 2202 76-77 0.07374324210216798 2202 78-79 0.16126189105658995 2202 80-81 0.2051898170497637 2202 82-83 0.1250606080510508 2202 84-85 0.07011574195357184 2202 86-87 0.19420382456724639 2202 88-89 0.11068747052033956 2202 90-91 0.1541729461340111 2202 92-93 0.06636755499607716 2202 94-95 0.1347280067428258 2202 96-97 0.040535565477405555 2202 98-99 0.12248009128106219 2202 100-101 -0.14945375307237896 1102 1 0.027475083362048736 1102 2 0.034364568348692615 1102 3 0.015880489763318906 1102 4 0.035958419548222764 1102 5 0.019607636343501156 1102 6 0.032514344342928325 1102 7 0.07537636351547405 1102 8 0.08533551899640202 1102 9 0.12103078396976485 1102 10-11 0.010430067067417781 1102 12-13 0.014807233677061049 1102 14-15 0.0016524276425471385 1102 16-17 0.024215646026149784 1102 18-19 0.07489601202583884 1102 20-21 0.20354627015925786 1102 22-23 0.07977671526040808 1102 24-25 -0.05931707548456444 1102 26-27 -0.0013038045770770168 1102 28-29 -0.07705269558649519 1102 30-31 0.08619137780048192 1102 32-33 0.02741926114732962 1102 34-35 -0.016707584885757853 1102 36-37 0.10724284552522789 1102 38-39 -0.0019433761531004734 1102 40-41 0.05707307346315815 1102 42-43 0.017036344309495632 1102 44-45 0.1266365254382933 1102 46-47 0.0017848651040353047 1102 48-49 0.03622961844956052 1102 50-51 0.005196316221955044 1102 52-53 0.17927850386367794 1102 54-55 0.2378467027696587 1102 56-57 0.2437417857196209 1102 58-59 0.20318886065862785 1102 60-61 0.35083010458713915 1102 62-63 0.31468787764296735 1102 64-65 0.2720716758192623 1102 66-67 0.21520240187815887 1102 68-69 0.11963894575058731 1102 70-71 0.14637050967756693 1102 72-73 0.2501654145053678 1102 74-75 0.2413181364933763 1102 76-77 0.20908572435094186 1102 78-79 0.06751761354814789 1102 80-81 0.24268763723866726 1102 82-83 0.09106140416953679 1102 84-85 0.03882394924261234 1102 86-87 0.10217594357447268 1102 88-89 0.16869602629839164 1102 90-91 0.20667550965425363 1102 92-93 0.2228942135788543 1102 94-95 0.19899585693902822 1102 96-97 0.12904962217839255 1102 98-99 0.053288051717210294 1102 100-101 0.16703118727956223 2305 1 0.16994731367293525 2305 2 0.1740566646192505 2305 3 0.13089148714719556 2305 4 0.17686362336525008 2305 5 0.206939237104514 2305 6 0.27500349370593824 2305 7 0.20963422137932497 2305 8 0.18978994862292353 2305 9 0.16978959309869168 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74-75 0.19436690364693732 2305 76-77 0.28837637294913776 2305 78-79 0.21391143843941762 2305 80-81 0.10276980973703331 2305 82-83 0.026995025088396574 2305 84-85 -0.025583976009698972 2305 86-87 0.0540682877609413 2305 88-89 -0.003239237034570408 2305 90-91 0.22758679627335 2305 92-93 0.20556124893506933 2305 94-95 0.23863060683054726 2305 96-97 0.31153699331448337 2305 98-99 0.3138733933727309 2305 100-101 0.2268925488834448 2203 1 0.19169726278835952 2203 2 0.11698136254495495 2203 3 0.21412091409141 2203 4 0.2232206971939945 2203 5 0.2910623499416687 2203 6 0.2618138849632814 2203 7 0.3004106626678009 2203 8 0.2543836168904363 2203 9 0.3271452923618554 2203 10-11 0.18360017919997773 2203 12-13 0.2684033465441047 2203 14-15 0.26468617664301064 2203 16-17 0.1453072281053096 2203 18-19 0.21450131362369973 2203 20-21 0.2676457179308258 2203 22-23 0.18319118414056845 2203 24-25 0.1475021194105679 2203 26-27 0.24460035180032236 2203 28-29 0.20430482261986072 2203 30-31 0.05901155750726872 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-0.8577440554331552 1213 100-101 -0.7072996615413629 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 267.0 3 114.0 4 119.0 5 119.0 6 538.0 7 1660.0 8 4698.0 9 10107.0 10 12274.0 11 10684.0 12 7636.0 13 6057.0 14 5439.0 15 5380.0 16 5638.0 17 5865.0 18 6304.0 19 6904.0 20 7905.0 21 8726.0 22 9877.0 23 10934.0 24 12488.0 25 14571.0 26 17000.0 27 20383.0 28 24378.0 29 30596.0 30 37958.0 31 49129.0 32 67008.0 33 96412.0 34 147009.0 35 248477.0 36 497482.0 37 958178.0 38 316324.0 39 1119.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.68495724889599 25.049666447430237 23.960687776003006 14.30468852767077 2 32.83627353972088 22.141683837652355 32.564982613554925 12.457060009071842 3 39.102130124783876 18.474205796097415 26.022627456962073 16.401036622156635 4 31.68251852597619 17.73910586948306 33.89815991206407 16.680215692476686 5 30.971204534662157 16.518171107758338 35.625409496101895 16.885214861477614 6 25.209626361012578 17.20096786455305 40.68253832319945 16.906867451234923 7 33.86273069603474 17.20849348936624 30.199382056816525 18.729393757782496 8 30.700573664593755 26.469032286890116 24.944291194385528 17.8861028541306 9 29.693268579130848 26.76348508769789 24.807267388925077 18.73597894424618 10-11 24.525328542901885 28.130802653156255 30.221592490619564 17.122276313322295 12-13 25.20038739905538 25.805609251470905 29.822030345758726 19.171973003714985 14-15 23.492683681442486 26.79401027224568 30.82723155131636 18.88607449499548 16-17 22.58778471333012 27.94064780782961 31.606457766603636 17.86510971223663 18-19 22.145957276905325 28.025343196946835 31.504908123214705 18.323791402933143 20-21 22.12515164865529 28.389548064012395 31.089912471296334 18.395387816035985 22-23 22.269621497562593 27.765751158247948 31.108166727623793 18.856460616565666 24-25 22.227244550543485 27.81995361202545 31.00809999604327 18.944701841387797 26-27 21.755219476263548 28.3367799413807 31.01943480644007 18.888565775915676 28-29 21.33861754599992 28.54682398861777 30.468589195596746 19.645969269785564 30-31 21.26304228239425 28.881682918327833 30.32823931208717 19.527035487190748 32-33 20.723645979980677 29.128877648773045 31.387492755002295 18.759983616243993 34-35 21.460735256401094 28.83482867107358 31.192683838544138 18.511752233981184 36-37 20.956090780731817 29.094499863393036 31.534095236017905 18.415314119857236 38-39 21.28702313391068 29.00751069732732 30.84068805931525 18.864778109446746 40-41 21.29960802928741 28.956558992889914 30.041483974562716 19.702349003259968 42-43 21.35229803756321 28.77097504815796 30.71019820009632 19.16652871418252 44-45 20.779247890825253 28.88390666613756 31.2839809919906 19.052864451046588 46-47 21.06488230697663 28.758691962819487 30.893407557921027 19.283018172282855 48-49 20.89917923023683 29.692907225181308 29.926386506575188 19.48152703800668 50-51 20.953901919899025 29.217941881036424 29.788936197428033 20.03922000163652 52-53 21.287887551787218 28.839655958880595 29.792681837205915 20.07977465212627 54-55 21.964191079702548 28.950853473641292 29.429128194249643 19.655827252406514 56-57 21.98894670149342 29.193076857534784 29.16206081213888 19.655915628832922 58-59 21.58962251021417 29.2449828140175 29.799323844995907 19.366070830772426 60-61 20.83167636218071 29.498542711498622 29.69670235947051 19.973078566850162 62-63 21.143445561769507 29.275460465731907 29.42609441808329 20.154999554415294 64-65 20.786670324065188 29.227571345122367 29.641642001474366 20.344116329338082 66-67 21.16452346019965 28.39108812689753 29.785389794324757 20.658998618578067 68-69 21.24465795090956 28.760625807606505 30.145008145637686 19.849708095846253 70-71 20.556472510558038 30.255861466952112 29.455362569898885 19.732303452590962 72-73 20.36964241367713 30.529638939537758 28.98821518533537 20.112503461449744 74-75 20.945198989260895 29.776964323316186 29.02355383088169 20.254282856541227 76-77 20.8546639595596 29.36729237339201 29.32660680272518 20.45143686432321 78-79 20.67842154632176 29.523667347973632 29.538971842550215 20.258939263154392 80-81 20.000447512600864 29.96959922802196 29.553318493965914 20.476634765411262 82-83 19.466627295501908 29.624124493127603 29.72230163327641 21.186946578094084 84-85 19.63520685465823 29.31487269759237 30.075377167939877 20.974543279809527 86-87 19.96926272285184 29.3585191944366 30.635158073932704 20.037060008778855 88-89 20.173667843183274 29.965874248127655 29.95812608887966 19.90233181980941 90-91 20.393401955463688 29.69069268636121 29.472896590293235 20.443008767881864 92-93 19.82632449195026 30.22564843963704 29.693360643640904 20.254666424771788 94-95 19.468852524353924 30.62124623742495 29.84813588152873 20.061765356692398 96-97 19.81044145480741 31.139080785620664 29.336456736195654 19.71402102337627 98-99 20.457492927475244 30.223942129871205 28.836345208781683 20.48221973387187 100-101 20.44801107851336 30.034877985347375 28.226984657409876 21.290126278729392 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 191.0 1 187.0 2 185.0 3 239.0 4 258.0 5 247.5 6 317.0 7 456.5 8 640.5 9 794.5 10 994.5 11 1120.0 12 1072.0 13 986.5 14 890.5 15 803.0 16 733.5 17 687.5 18 687.5 19 746.0 20 860.5 21 1045.0 22 1296.5 23 1704.0 24 2339.0 25 3376.0 26 5356.0 27 8263.5 28 11223.5 29 14099.5 30 17480.5 31 20926.0 32 26595.0 33 37808.5 34 54928.5 35 79470.5 36 114053.0 37 152218.0 38 186494.5 39 206643.0 40 205119.0 41 186994.5 42 167486.5 43 159259.5 44 147726.0 45 133203.0 46 125045.0 47 111076.5 48 92512.0 49 77767.0 50 64462.5 51 52941.5 52 43141.5 53 34374.0 54 28211.0 55 22215.0 56 15863.0 57 10732.0 58 7449.5 59 5446.0 60 4053.0 61 3050.5 62 2216.5 63 1591.5 64 1223.5 65 933.0 66 724.0 67 577.0 68 443.5 69 330.0 70 236.5 71 196.0 72 155.0 73 107.5 74 77.0 75 70.0 76 64.0 77 45.5 78 41.0 79 35.5 80 30.5 81 29.0 82 23.0 83 22.0 84 22.0 85 16.5 86 13.5 87 12.5 88 9.5 89 8.0 90 11.5 91 9.0 92 8.0 93 7.5 94 4.5 95 4.5 96 3.5 97 2.0 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1878265723394893 2 0.17916111633581006 3 0.15136413409024152 4 0.14326137003485315 5 0.14813803358670727 6 0.12758102107581448 7 0.10237242179238393 8 0.0883051230851124 9 0.08823009749200696 10-11 0.06885473807252498 12-13 0.08828636668683605 14-15 0.09243153070591205 16-17 0.08502275338674906 18-19 0.08112142254526575 20-21 0.07939583390384045 22-23 0.10064683315095863 24-25 0.4521042240534302 26-27 0.2846471002420701 28-29 0.1260805092137055 30-31 0.2338547737096817 32-33 0.26356490857943915 34-35 0.2476969956376369 36-37 0.25015408381184034 38-39 0.2681789825554242 40-41 0.2678413673864497 42-43 0.2939877865836984 44-45 0.2698483020020204 46-47 0.2887735078628697 48-49 0.27298062051417293 50-51 0.2878919571438807 52-53 0.286766573247299 54-55 0.263865010951861 56-57 0.2796766546988341 58-59 0.2699045711968495 60-61 0.2452399074634335 62-63 0.23745600217874321 64-65 0.23661196425630696 66-67 0.24535244585309163 68-69 0.2500227890239058 70-71 0.2359742467149106 72-73 0.22903437935265666 74-75 0.2369683358235578 76-77 0.23773734815288866 78-79 0.24036324391157934 80-81 0.24810963639971687 82-83 0.2737308764452274 84-85 0.26735370103126427 86-87 0.2661908043381298 88-89 0.2648778564587845 90-91 0.25688763079305427 92-93 0.23256058222861273 94-95 0.23166027511134735 96-97 0.2269899319405332 98-99 0.25131698050497475 100-101 0.2594572573569159 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2665757.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.95418145396606 #Duplication Level Percentage of deduplicated Percentage of total 1 88.23270943496992 43.193600678545806 2 5.217146692599532 5.108022917229528 3 1.8683298254271952 2.7438767186945894 4 0.9664228279152843 1.8924175391607938 5 0.6638086048863171 1.6248103447154414 6 0.42712422649382276 1.2545710132298098 7 0.2841283447474257 0.9736489381486354 8 0.2635098263172013 1.0319926281948284 9 0.19337263225778545 0.8519759035000813 >10 1.5688746508207496 15.060328025555611 >50 0.16173644310714805 5.495099940619337 >100 0.13629168758333116 13.99054244110931 >500 0.013965728095541868 4.428827867101426 >1k 0.0024244717118435664 1.880888746377729 >5k 1.546030668619762E-4 0.4693962978171866 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 6293 0.23606802870629243 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 6111 0.22924069973369662 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 2940 0.1102876218650087 No Hit TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA 2862 0.10736162373389621 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 2802 0.10511085594073277 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 3.7512796552724046E-4 0.0 0.0 2 4.1264076207996454E-4 0.0 0.0 3 4.8766635518541265E-4 0.0 0.0 4 4.8766635518541265E-4 0.0 0.0 5 4.8766635518541265E-4 0.0 0.0 6 5.626919482908607E-4 0.0 3.7512796552724045E-5 7 6.377175413963089E-4 0.0 3.7512796552724045E-5 8 8.627943207126531E-4 0.0 3.7512796552724045E-5 9 0.0011628966931344455 0.0 3.7512796552724045E-5 10-11 0.0018756398276362023 0.0 3.7512796552724045E-5 12-13 0.0025508701655852354 0.0 3.7512796552724045E-5 14-15 0.003751279655272405 0.0 3.7512796552724045E-5 16-17 0.005233035119105005 0.0 3.7512796552724045E-5 18-19 0.007033649353635759 0.0 3.7512796552724045E-5 20-21 0.00879675079161379 0.0 3.7512796552724045E-5 22-23 0.011347620957199024 0.0 3.7512796552724045E-5 24-25 0.013560875953809743 0.0 3.7512796552724045E-5 26-27 0.016524386881474944 0.0 3.7512796552724045E-5 28-29 0.019675461791903763 0.0 3.7512796552724045E-5 30-31 0.030422878004259205 0.0 3.7512796552724045E-5 32-33 0.034943169988862446 0.0 3.7512796552724045E-5 34-35 0.038694449644134855 0.0 3.7512796552724045E-5 36-37 0.0429709084511454 0.0 3.7512796552724045E-5 38-39 0.047997623189210414 0.0 3.7512796552724045E-5 40-41 0.05354951707901358 1.8756398276362023E-5 3.7512796552724045E-5 42-43 0.05870752660501313 3.7512796552724045E-5 3.7512796552724045E-5 44-45 0.064503253672409 3.7512796552724045E-5 3.7512796552724045E-5 46-47 0.07103048027258299 3.7512796552724045E-5 3.7512796552724045E-5 48-49 0.07883314195554958 3.7512796552724045E-5 3.7512796552724045E-5 50-51 0.08665456003679255 3.7512796552724045E-5 3.7512796552724045E-5 52-53 0.09503867006632638 3.7512796552724045E-5 3.7512796552724045E-5 54-55 0.10400422844242743 3.7512796552724045E-5 3.7512796552724045E-5 56-57 0.11356999156337205 3.7512796552724045E-5 3.7512796552724045E-5 58-59 0.12469253574125473 3.7512796552724045E-5 3.7512796552724045E-5 60-61 0.13654657945191553 3.7512796552724045E-5 3.7512796552724045E-5 62-63 0.14894455871259082 3.7512796552724045E-5 3.7512796552724045E-5 64-65 0.1619990119129388 3.7512796552724045E-5 3.7512796552724045E-5 66-67 0.1762726310012503 3.7512796552724045E-5 3.7512796552724045E-5 68-69 0.19349100461895063 3.7512796552724045E-5 3.7512796552724045E-5 70-71 0.21224740289531266 3.7512796552724045E-5 5.6269194829086064E-5 72-73 0.23383601731140535 3.7512796552724045E-5 7.502559310544809E-5 74-75 0.25735654074996334 3.7512796552724045E-5 7.502559310544809E-5 76-77 0.2841406774886083 3.7512796552724045E-5 7.502559310544809E-5 78-79 0.3134944407911149 3.7512796552724045E-5 7.502559310544809E-5 80-81 0.3469558553161447 3.7512796552724045E-5 7.502559310544809E-5 82-83 0.3849563182240542 3.7512796552724045E-5 7.502559310544809E-5 84-85 0.4263516892199852 3.7512796552724045E-5 7.502559310544809E-5 86-87 0.4745368763919592 3.7512796552724045E-5 7.502559310544809E-5 88-89 0.5296806873244635 3.7512796552724045E-5 7.502559310544809E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 490 0.0 63.90976 2 CGGACGG 40 7.589604E-6 59.320225 1 CGTTGCG 25 0.0050437558 56.93778 2 CGGGAGA 505 0.0 55.426144 4 GAGCGGC 405 0.0 53.8553 9 CGGAAAG 285 0.0 51.602516 5 GATCCGG 230 0.0 45.392864 1 AGAGCGG 450 0.0 45.3087 8 CGCGAGG 75 1.3736462E-7 44.292435 1 GTTGCGC 45 9.6711906E-4 42.18327 1 GGTACGG 70 4.339776E-6 40.676723 1 CGAGGCG 95 1.945773E-8 39.963097 1 ATCCGGA 215 0.0 39.724033 2 GGTCCGG 120 8.731149E-11 39.546814 1 GAGAGCG 600 0.0 39.512657 7 TTGTGTT 32830 0.0 38.927654 1 TCGGAAA 270 0.0 38.655643 4 AAGCGGC 225 0.0 35.82548 9 GTGACGC 80 1.084981E-5 35.592136 1 GTTGACG 120 3.8326107E-9 35.592133 1 >>END_MODULE