##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n02_34344-1.34200000035854.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4870057 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.697695530052318 33.0 33.0 33.0 27.0 33.0 2 31.568731331070662 33.0 33.0 33.0 27.0 33.0 3 31.508148877928946 33.0 33.0 33.0 27.0 33.0 4 35.15115675237477 37.0 37.0 37.0 33.0 37.0 5 35.17005858452991 37.0 37.0 37.0 33.0 37.0 6 35.285981252375485 37.0 37.0 37.0 33.0 37.0 7 35.09052871455098 37.0 37.0 37.0 33.0 37.0 8 35.16605472995491 37.0 37.0 37.0 33.0 37.0 9 34.9717198381867 37.0 37.0 37.0 33.0 37.0 10-11 34.918465225355675 37.0 37.0 37.0 33.0 37.0 12-13 34.90571188386501 37.0 37.0 37.0 33.0 37.0 14-15 36.567044697834135 40.0 37.0 40.0 33.0 40.0 16-17 36.52177048441117 40.0 37.0 40.0 33.0 40.0 18-19 36.514170984035715 40.0 37.0 40.0 33.0 40.0 20-21 36.43801817925334 40.0 37.0 40.0 33.0 40.0 22-23 36.652400988325184 40.0 37.0 40.0 33.0 40.0 24-25 36.33647593857731 40.0 37.0 40.0 33.0 40.0 26-27 36.27102454858331 40.0 37.0 40.0 30.0 40.0 28-29 36.19925772942699 40.0 37.0 40.0 30.0 40.0 30-31 36.14701018899779 40.0 37.0 40.0 27.0 40.0 32-33 36.04765857976611 40.0 37.0 40.0 27.0 40.0 34-35 36.01245467968856 40.0 37.0 40.0 27.0 40.0 36-37 35.9120329597785 40.0 37.0 40.0 27.0 40.0 38-39 35.82247302238967 40.0 37.0 40.0 27.0 40.0 40-41 35.75394466635606 37.0 37.0 40.0 27.0 40.0 42-43 35.64634253767461 37.0 37.0 40.0 27.0 40.0 44-45 35.47467278103727 37.0 37.0 40.0 27.0 40.0 46-47 35.3902863970586 37.0 37.0 40.0 27.0 40.0 48-49 35.27562685611277 37.0 37.0 40.0 27.0 40.0 50-51 34.13889426756196 37.0 35.0 38.5 24.5 38.5 52-53 34.559136679509095 37.0 35.0 38.5 27.0 40.0 54-55 35.31523255271961 37.0 37.0 40.0 27.0 40.0 56-57 35.40108657865811 37.0 37.0 40.0 27.0 40.0 58-59 35.140078339945504 37.0 37.0 40.0 27.0 40.0 60-61 34.904877088707586 37.0 35.0 40.0 27.0 40.0 62-63 34.648544873294085 37.0 33.0 40.0 27.0 40.0 64-65 34.41844469171511 37.0 33.0 40.0 27.0 40.0 66-67 34.199379288579166 37.0 33.0 37.0 27.0 40.0 68-69 34.047553550194586 37.0 33.0 37.0 27.0 40.0 70-71 33.66597054202856 37.0 33.0 37.0 22.0 40.0 72-73 33.53385607191045 37.0 33.0 37.0 24.5 38.5 74-75 33.35905914448229 37.0 33.0 37.0 27.0 37.0 76-77 33.09858590977477 37.0 33.0 37.0 22.0 37.0 78-79 32.81545298135114 37.0 33.0 37.0 22.0 37.0 80-81 32.53238401521789 37.0 33.0 37.0 22.0 37.0 82-83 32.31044605843422 37.0 33.0 37.0 22.0 37.0 84-85 32.13896675131318 37.0 33.0 37.0 22.0 37.0 86-87 31.995721610650552 35.0 33.0 37.0 22.0 37.0 88-89 31.856958553051843 33.0 33.0 37.0 22.0 37.0 90-91 31.702809227900207 33.0 33.0 37.0 15.0 37.0 92-93 31.558261022406924 33.0 33.0 37.0 15.0 37.0 94-95 31.437975674617363 33.0 33.0 37.0 15.0 37.0 96-97 31.297995896146595 33.0 33.0 37.0 15.0 37.0 98-99 31.152596879256237 33.0 33.0 37.0 15.0 37.0 100-101 29.92328642149363 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.2729597672664035 2306 2 0.2322524197512834 2306 3 0.21646507016112082 2306 4 0.3684506831125489 2306 5 0.2846815085881147 2306 6 0.23621358379751456 2306 7 0.41895012982488566 2306 8 0.24240656606010447 2306 9 0.3377940370152501 2306 10-11 0.3116790931699356 2306 12-13 0.2547318330246924 2306 14-15 0.5066094415062636 2306 16-17 0.48969612901522197 2306 18-19 0.5004510636719814 2306 20-21 0.5367407058737044 2306 22-23 0.4881724289409206 2306 24-25 0.43107517316589394 2306 26-27 0.4622509425505541 2306 28-29 0.4401451310027866 2306 30-31 0.5044284270500157 2306 32-33 0.36285656683424605 2306 34-35 0.430293744317531 2306 36-37 0.3920307243757222 2306 38-39 0.37134696658714006 2306 40-41 0.3154112242060805 2306 42-43 0.2163300953345697 2306 44-45 0.3025533743500617 2306 46-47 0.3177349552554105 2306 48-49 0.2431257348615361 2306 50-51 0.20764846584147278 2306 52-53 0.157207129961769 2306 54-55 0.4212157052503258 2306 56-57 0.3216091701559449 2306 58-59 0.43509514903851 2306 60-61 0.49399673380256814 2306 62-63 0.42647385902533586 2306 64-65 0.3582023881909109 2306 66-67 0.43422267689432914 2306 68-69 0.3567722886507525 2306 70-71 0.35128419460038174 2306 72-73 0.2726731482829976 2306 74-75 0.2336084238906082 2306 76-77 0.36746109948819594 2306 78-79 0.41108723082330556 2306 80-81 0.3994204506603154 2306 82-83 0.42266633997563474 2306 84-85 0.30367723026602533 2306 86-87 0.41824315149591484 2306 88-89 0.49238891592746015 2306 90-91 0.4094256382862831 2306 92-93 0.3233023418009431 2306 94-95 0.3336088108195021 2306 96-97 0.478712741478855 2306 98-99 0.45833364794459897 2306 100-101 0.08284657125660644 2307 1 0.23548516678619436 2307 2 0.15320036731629472 2307 3 0.13888306599723066 2307 4 0.2809710844297655 2307 5 0.24707157216140985 2307 6 0.1561465117638079 2307 7 0.3017094067772135 2307 8 0.20786648720384449 2307 9 0.21914266741162436 2307 10-11 0.2892023684536369 2307 12-13 0.18066101810620694 2307 14-15 0.41700651986462844 2307 16-17 0.3872381667824101 2307 18-19 0.388272991171128 2307 20-21 0.4904914243653238 2307 22-23 0.2792643343518577 2307 24-25 0.25778922022973916 2307 26-27 0.30821518233355505 2307 28-29 0.30516943956875053 2307 30-31 0.17603844421691406 2307 32-33 0.11694057892989207 2307 34-35 0.17212802698895757 2307 36-37 0.10308126966040021 2307 38-39 0.15222120027618047 2307 40-41 0.12613501199776778 2307 42-43 0.14579988216122786 2307 44-45 0.1359185872446531 2307 46-47 0.21712382887201187 2307 48-49 0.14113433561472988 2307 50-51 0.09389221087931077 2307 52-53 0.0602819857347896 2307 54-55 0.03670393352208379 2307 56-57 0.016538637828141134 2307 58-59 0.14113273744421662 2307 60-61 0.14229268314385735 2307 62-63 0.15093366867071012 2307 64-65 0.1475528525431571 2307 66-67 0.042059618225046336 2307 68-69 0.00504593831903577 2307 70-71 -0.029391889192559972 2307 72-73 -0.05696345355440968 2307 74-75 0.0017476219097858348 2307 76-77 -0.010208805757415007 2307 78-79 0.06457727382706935 2307 80-81 0.05654284126215714 2307 82-83 -0.07234958698909111 2307 84-85 -0.057050979012565506 2307 86-87 -0.04166352629495407 2307 88-89 0.12194122115615968 2307 90-91 0.15792276528099336 2307 92-93 0.023559556994708686 2307 94-95 -0.0313130826534227 2307 96-97 0.08678358907789274 2307 98-99 0.01623796889071727 2307 100-101 -0.07923494942730258 2201 1 0.12015864879478144 2201 2 0.08519033648498464 2201 3 0.045692762118530794 2201 4 0.20197641840320557 2201 5 0.1118467832807184 2201 6 0.07304947951094931 2201 7 0.1988369278517581 2201 8 0.13371206743890696 2201 9 0.12863636243516652 2201 10-11 0.11657414707541847 2201 12-13 0.10399468420530411 2201 14-15 0.2111954762289443 2201 16-17 0.19400127569701908 2201 18-19 0.29419908202590506 2201 20-21 0.3555434106030262 2201 22-23 0.3252519083548506 2201 24-25 0.24456645486399964 2201 26-27 0.24729063192253875 2201 28-29 0.18962512825553546 2201 30-31 0.23903118992330974 2201 32-33 0.16556943443253402 2201 34-35 0.15881608787686474 2201 36-37 0.09348004268947108 2201 38-39 0.12591616025829921 2201 40-41 0.10120758484543302 2201 42-43 0.21430700521801072 2201 44-45 0.19345958286485399 2201 46-47 0.14577398886130766 2201 48-49 0.12862944694917644 2201 50-51 0.011641394481515022 2201 52-53 0.20389527602542756 2201 54-55 0.31009035608076374 2201 56-57 0.2728889489454289 2201 58-59 0.21721747904972943 2201 60-61 0.23567993574747703 2201 62-63 0.2476147414776193 2201 64-65 0.21098473688723374 2201 66-67 0.3600630124358162 2201 68-69 0.28322680511028864 2201 70-71 0.19739414690915424 2201 72-73 0.2540552246999752 2201 74-75 0.3340399405543124 2201 76-77 0.3252834856676188 2201 78-79 0.27784657031538273 2201 80-81 0.23566008847421216 2201 82-83 0.17560745556828294 2201 84-85 0.15106437405191286 2201 86-87 0.030276412102253403 2201 88-89 0.13571482602634788 2201 90-91 0.0932103870676535 2201 92-93 0.07006787202592335 2201 94-95 0.19547267302771232 2201 96-97 0.20898340257950565 2201 98-99 0.18568986629118456 2201 100-101 0.001636104213790901 1101 1 0.2632365141149755 1101 2 0.20612849037691916 1101 3 0.21810397276797033 1101 4 0.3036261400063367 1101 5 0.28576223173718773 1101 6 0.3004832882990627 1101 7 0.42488254265325054 1101 8 0.27612382623503606 1101 9 0.32385813942010344 1101 10-11 0.39182063397996103 1101 12-13 0.38652219192225346 1101 14-15 0.44413416060691446 1101 16-17 0.5056441196419925 1101 18-19 0.5940376536589298 1101 20-21 0.5982851736993595 1101 22-23 0.523515399636068 1101 24-25 0.4620335739766048 1101 26-27 0.525079711407642 1101 28-29 0.4560591991210714 1101 30-31 0.5182221670622553 1101 32-33 0.3950436734541469 1101 34-35 0.3833895372840104 1101 36-37 0.31954877461958375 1101 38-39 0.37942754796411293 1101 40-41 0.29440042768244723 1101 42-43 0.400890556852012 1101 44-45 0.3786933441024871 1101 46-47 0.4085366015250287 1101 48-49 0.3994466503580867 1101 50-51 0.43263646232758646 1101 52-53 0.4389961825001123 1101 54-55 0.5366631247197091 1101 56-57 0.5045099986446857 1101 58-59 0.5009087988220173 1101 60-61 0.5097597782277745 1101 62-63 0.5281631314175144 1101 64-65 0.4389574038607975 1101 66-67 0.45737650219255954 1101 68-69 0.4603831083890242 1101 70-71 0.5344554608650895 1101 72-73 0.46600330637816256 1101 74-75 0.45742913486697745 1101 76-77 0.5611875769911876 1101 78-79 0.5675653377417404 1101 80-81 0.5714298838588832 1101 82-83 0.5129660016500068 1101 84-85 0.557829203960658 1101 86-87 0.6277967064420267 1101 88-89 0.4431987944017912 1101 90-91 0.5319993544242685 1101 92-93 0.6029899120712905 1101 94-95 0.48184824547862704 1101 96-97 0.6111356780630395 1101 98-99 0.5151644718546571 1101 100-101 0.315391020664741 2304 1 0.08850552228208741 2304 2 0.02153122878089775 2304 3 0.04552055216577955 2304 4 0.14133180491965902 2304 5 0.11105719478987908 2304 6 0.10194021780196039 2304 7 0.12350713993475892 2304 8 0.03347814306226837 2304 9 -0.01575172522836965 2304 10-11 0.05894133337097429 2304 12-13 0.05642244623035708 2304 14-15 0.1566417061899088 2304 16-17 0.048042562974615066 2304 18-19 0.1496657288239973 2304 20-21 0.16947231389975315 2304 22-23 0.16921211871809305 2304 24-25 0.13158173070684853 2304 26-27 0.08487649674216868 2304 28-29 0.0563818361260644 2304 30-31 0.010627020896073702 2304 32-33 -0.05875535025817413 2304 34-35 -0.2442376748988906 2304 36-37 0.057767798725841146 2304 38-39 -0.009198011425894492 2304 40-41 -0.03731236081588207 2304 42-43 -0.02806807696245528 2304 44-45 0.0033334689476234303 2304 46-47 0.04944208428483421 2304 48-49 0.053036072369806675 2304 50-51 0.030625257354593316 2304 52-53 -0.02616304574522843 2304 54-55 0.154770333756737 2304 56-57 0.15347294452561044 2304 58-59 0.1396168627489729 2304 60-61 0.1922715469906464 2304 62-63 0.21415633920172894 2304 64-65 0.14271754857670516 2304 66-67 0.03214609214020925 2304 68-69 0.15455797164410967 2304 70-71 0.2728717776563414 2304 72-73 0.1563395487208581 2304 74-75 0.20646786190286548 2304 76-77 0.2086141714193701 2304 78-79 0.21194920182404076 2304 80-81 0.23435609596803886 2304 82-83 -0.008603539346019318 2304 84-85 0.03745383658347379 2304 86-87 0.1342038299417112 2304 88-89 0.15520313519640538 2304 90-91 -0.04660757646881919 2304 92-93 0.07883558446388506 2304 94-95 0.17181336851225026 2304 96-97 0.0848684094421337 2304 98-99 0.05925179603608299 2304 100-101 0.13994225180451636 2202 1 -0.006410201694723838 2202 2 0.0067243804341003965 2202 3 -0.053141623505560176 2202 4 0.04827380343937904 2202 5 -0.019779027238776337 2202 6 -0.07000667958518392 2202 7 0.046348494529503625 2202 8 -0.0546394573176201 2202 9 -0.08948604411544636 2202 10-11 -0.015726918848251614 2202 12-13 0.044220735004955714 2202 14-15 0.05177981712002122 2202 16-17 0.05945503880804637 2202 18-19 0.09981499163966845 2202 20-21 0.22748345985193197 2202 22-23 0.1716813622713289 2202 24-25 0.014672876819808778 2202 26-27 0.03174995896767996 2202 28-29 0.025780901369714115 2202 30-31 0.09762589183105774 2202 32-33 0.04589159729476222 2202 34-35 -0.20563863805890747 2202 36-37 -0.002796040914390119 2202 38-39 -0.09685634896522544 2202 40-41 -0.009046498639143863 2202 42-43 -0.08938325491507726 2202 44-45 -0.1088801664598904 2202 46-47 -0.08925749465564792 2202 48-49 -0.12746915312068552 2202 50-51 0.0014124466870626406 2202 52-53 -0.029758004821168527 2202 54-55 0.08204574236093976 2202 56-57 0.05863062221555282 2202 58-59 0.019339953764934137 2202 60-61 0.09591697808620125 2202 62-63 0.10635216298183536 2202 64-65 -0.002943417641873225 2202 66-67 0.07399116696493024 2202 68-69 0.17316017423002705 2202 70-71 0.10757026715148044 2202 72-73 0.07263944630303598 2202 74-75 0.17390642317660365 2202 76-77 0.10438763263046269 2202 78-79 0.14084853856773094 2202 80-81 0.1853439752187569 2202 82-83 0.09013758857442156 2202 84-85 0.16608188382927125 2202 86-87 0.11500072896531321 2202 88-89 0.07461805787966469 2202 90-91 0.13567479237427094 2202 92-93 0.03508665988922033 2202 94-95 0.13420549342255583 2202 96-97 0.1287387769308772 2202 98-99 0.18101846218597117 2202 100-101 0.01989569198847363 1102 1 -0.004184308162162864 1102 2 0.03546298173232998 1102 3 0.05124126324070133 1102 4 -0.004019092912450617 1102 5 0.04064022740651296 1102 6 0.019208185679694623 1102 7 0.1365257715922752 1102 8 0.05808888567609216 1102 9 0.10365478999164424 1102 10-11 0.15722409783203517 1102 12-13 0.052863634856620934 1102 14-15 0.09119729317568925 1102 16-17 0.12507529769445824 1102 18-19 0.10706327145086192 1102 20-21 0.20594861037425716 1102 22-23 0.04395138742795979 1102 24-25 -0.13892802925733605 1102 26-27 -0.09953972512814602 1102 28-29 -0.11529642300404674 1102 30-31 -0.07680112653926585 1102 32-33 -0.096748845084079 1102 34-35 -0.08243314559872772 1102 36-37 0.010030466058708498 1102 38-39 -0.08957568516083825 1102 40-41 -0.06628259228622824 1102 42-43 -0.1512597892543397 1102 44-45 -0.07957568861382924 1102 46-47 -0.0925264226358351 1102 48-49 -0.06505583722748298 1102 50-51 -0.21662678685802916 1102 52-53 -0.027675309258533787 1102 54-55 0.06661350460171178 1102 56-57 0.0812361674683828 1102 58-59 0.08119093510956077 1102 60-61 0.15544769691808114 1102 62-63 0.09166475706619792 1102 64-65 0.14738881345502364 1102 66-67 0.21679707130902415 1102 68-69 0.18194121205267777 1102 70-71 0.0578716322548658 1102 72-73 0.06755617137052639 1102 74-75 0.18739427201082748 1102 76-77 0.12535719589352112 1102 78-79 0.034080452655693705 1102 80-81 0.0057881419254854904 1102 82-83 0.04630340811293365 1102 84-85 0.02805067881582346 1102 86-87 0.08525164587929623 1102 88-89 0.2185971229773891 1102 90-91 0.265813411045162 1102 92-93 0.0751596588072232 1102 94-95 0.13322469696185735 1102 96-97 0.034386073078277235 1102 98-99 -0.021665361250423842 1102 100-101 0.09819360954591261 2305 1 0.13900808822066324 2305 2 0.15011389121500684 2305 3 0.13263628107650405 2305 4 0.20331839568380872 2305 5 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-0.8338791354236363 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 499.0 3 258.0 4 282.0 5 309.0 6 1073.0 7 4672.0 8 14472.0 9 35213.0 10 48026.0 11 41697.0 12 27340.0 13 19509.0 14 15361.0 15 13668.0 16 13203.0 17 13736.0 18 14645.0 19 16228.0 20 18045.0 21 19739.0 22 22114.0 23 23970.0 24 26822.0 25 30765.0 26 35781.0 27 42791.0 28 51444.0 29 62602.0 30 76651.0 31 98220.0 32 132895.0 33 188616.0 34 282171.0 35 473230.0 36 921342.0 37 1599286.0 38 482986.0 39 396.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 34.86947396011316 24.148536364124517 26.331044247408915 14.650945428353406 2 29.645682913794047 22.951801701910217 34.82884418136868 12.57367120292706 3 38.518353232239825 19.51779899002467 26.60475743419787 15.359090343537629 4 28.227785576124635 18.49535272907638 36.73577286095778 16.541088833841197 5 32.915019405192595 17.516723508498306 33.74150868585963 15.82674840044947 6 25.276254254869645 18.39343524116668 40.88153805506066 15.44877244890301 7 32.85931736355853 18.774382992981234 31.79010918195245 16.576190461507785 8 27.765485929916256 27.96591898205002 28.00885424106432 16.259740846969407 9 31.69509556318483 25.495697025900053 25.19697986135775 17.61222754955737 10-11 25.342946112489695 28.71332996884008 28.828320019200625 17.1154038994696 12-13 26.462413319429984 25.216907596320908 28.80202182985138 19.51865725439773 14-15 24.404727648228704 25.714968104427065 30.182013182462082 19.69829106488215 16-17 23.688862111498192 26.9287197209077 31.32813928225746 18.05427888533665 18-19 22.974632087585434 27.33936354563824 31.357247845794923 18.328756520981408 20-21 22.660110316274306 28.17268723263655 30.650513361454777 18.51668908963436 22-23 23.76790478885173 27.27324489158076 30.294025541351072 18.66482477821644 24-25 22.511927430232603 28.40083632795426 30.241030112468202 18.846206129344935 26-27 22.10068590320336 28.85867659211937 29.908804343058275 19.131833161618992 28-29 21.98504820999364 28.629925002564743 29.637134452007214 19.747892335434404 30-31 22.5489695670706 28.174061282424013 29.686209489083993 19.59075966142139 32-33 22.42972019427402 27.785380367503265 30.52956984211067 19.255329596112052 34-35 21.561576693865845 28.038617952073995 30.215653333440372 20.184152020619788 36-37 20.61646553938779 28.51470125682592 30.355260817727302 20.513572386058982 38-39 21.057577160403277 28.721502773223424 30.441077327736547 19.779842738636752 40-41 21.474213073540078 28.073685226945415 30.696560679402268 19.755541020112236 42-43 21.965020735035754 28.187106899782137 30.833174244937645 19.014698120244468 44-45 21.116194070828946 28.89125913712853 30.958144884186 19.034401907856523 46-47 21.792326854013442 28.955056893585812 30.033816652438677 19.218799599962068 48-49 21.142087390959475 28.93456204403984 30.12086531349264 19.802485251508052 50-51 20.7843193380032 28.47621834494211 30.313908538875673 20.425553778179015 52-53 22.279554224406088 28.092496880997906 29.51387841384908 20.114070480746925 54-55 22.930266101658976 28.349400971202186 29.120264658464134 19.600068268674704 56-57 23.104171340400654 28.299122544301337 28.725758313446693 19.87094780185132 58-59 22.416004079854044 28.175050774273576 29.17857421907371 20.230370926798678 60-61 21.104114705645642 28.5163627172071 29.009023695769365 21.37049888137789 62-63 21.975237035936264 28.485785665094042 29.15048041121768 20.388496887752012 64-65 21.24065272640305 28.914411369775383 29.703202709567044 20.14173319425452 66-67 21.155134420767048 28.43238226366513 29.266693926439576 21.14578938912825 68-69 21.27868247400886 27.921140043847682 30.678257830174783 20.12191965196867 70-71 21.06828846764773 29.757165160601296 29.49084531312984 19.683701058621136 72-73 20.701215546749076 31.32543658757847 28.353609595953778 19.619738269718678 74-75 21.195663359815583 30.898602478080107 28.13355699172601 19.7721771703783 76-77 21.28323136322158 30.324080434592222 28.505362165502067 19.88732603668413 78-79 21.095186932430988 30.127530647697615 29.029071059846373 19.748211360025028 80-81 20.576580389292833 30.444489409523207 28.890236926397634 20.088693274786333 82-83 19.89581730143133 30.32720264821713 29.179864943396982 20.597115106954554 84-85 19.98047004078315 29.91948163872952 29.313149081365154 20.786899239122178 86-87 19.983062122091486 29.938970522080698 29.858367881978793 20.219599473849023 88-89 20.145554863580916 30.208295803717107 29.5762130000875 20.06993633261448 90-91 20.38131830172218 29.764162848309706 29.296799250011425 20.557719599956688 92-93 19.99488558161073 30.531575359205565 29.088455255270706 20.38508380391299 94-95 19.67637975382774 30.982414855787578 29.08926018661937 20.251945203765317 96-97 20.086692607564434 31.107262679629912 28.81691087228198 19.989133840523674 98-99 20.501442849580833 30.392545453853053 28.454617885447508 20.651393811118606 100-101 20.503425616363906 30.320873614519346 27.979226024802777 21.19647474431397 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 150.0 1 205.0 2 283.5 3 397.5 4 479.5 5 505.0 6 603.0 7 817.5 8 1119.5 9 1413.0 10 1678.5 11 1891.0 12 1976.0 13 1880.5 14 1705.0 15 1604.0 16 1520.0 17 1451.5 18 1539.0 19 1732.5 20 1970.5 21 2428.5 22 3097.5 23 4015.0 24 5330.0 25 7164.5 26 10082.5 27 14209.0 28 18817.5 29 23421.5 30 29069.0 31 36127.5 32 48836.0 33 70622.0 34 103013.0 35 142890.5 36 194485.0 37 257907.0 38 315599.0 39 339904.0 40 334600.0 41 310617.5 42 286249.5 43 287757.0 44 283870.5 45 264789.5 46 250373.0 47 222648.5 48 180193.5 49 149871.0 50 127755.0 51 109064.5 52 93096.5 53 75936.0 54 58914.5 55 44522.5 56 33914.0 57 25449.0 58 19696.5 59 15138.5 60 11311.0 61 8517.0 62 6251.0 63 4586.0 64 3383.5 65 2417.0 66 1756.5 67 1268.5 68 927.5 69 684.0 70 510.5 71 404.0 72 297.5 73 226.0 74 198.0 75 158.0 76 130.0 77 114.5 78 93.0 79 84.0 80 64.5 81 54.5 82 51.0 83 34.0 84 29.5 85 31.5 86 27.0 87 24.5 88 20.0 89 16.5 90 12.5 91 10.0 92 11.5 93 8.5 94 6.0 95 6.0 96 4.5 97 2.0 98 1.5 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19416610524271072 2 0.18338594394275057 3 0.15527539000056878 4 0.1479038130354532 5 0.1527908194914351 6 0.1337150674006485 7 0.10763734387503063 8 0.0942904775036514 9 0.09414674201965192 10-11 0.07287389038773057 12-13 0.09370526874736784 14-15 0.0986641429453495 16-17 0.09089215998909253 18-19 0.08675463141396497 20-21 0.08497847150454296 22-23 0.10518357382675399 24-25 0.45580575340288626 26-27 0.2900171394297849 28-29 0.1236946508018284 30-31 0.23149626380142985 32-33 0.26270739746988586 34-35 0.24651662187937431 36-37 0.24855971911622388 38-39 0.26659852235815723 40-41 0.26619811636701585 42-43 0.29293291639091695 44-45 0.2687442877978635 46-47 0.28783030670893583 48-49 0.2711364569244262 50-51 0.28667015601665446 52-53 0.28616708182265627 54-55 0.2623788592207443 56-57 0.2786928366546839 58-59 0.26735826705929727 60-61 0.24328257348938628 62-63 0.23636273661684043 64-65 0.2345147089654187 66-67 0.24353924399652818 68-69 0.24852891865536686 70-71 0.23460711034798978 72-73 0.2260548490500214 74-75 0.23525392002598736 76-77 0.23666047440512503 78-79 0.23853930251740382 80-81 0.2457055430768059 82-83 0.2711364569244262 84-85 0.2649660979327346 86-87 0.2648018288081639 88-89 0.2624609937830297 90-91 0.25426807119505995 92-93 0.23089052140457494 94-95 0.22962770250943673 96-97 0.2245250928274556 98-99 0.24918599515365014 100-101 0.25743025184304824 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4870057.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.6403169912426 #Duplication Level Percentage of deduplicated Percentage of total 1 92.36198719093252 48.619642836717766 2 3.6966382656077394 3.8918442020709736 3 1.1167352581291445 1.7635589394964588 4 0.6154816954681058 1.2959660620699411 5 0.35263698084975337 0.9281461227382881 6 0.2657861080817019 0.8394638988773674 7 0.18546263821148742 0.6833968445839389 8 0.16243018359787165 0.6840301082830158 9 0.1303014328304785 0.6173197855748543 >10 0.851978285637115 9.116155383349803 >50 0.11354973454940578 4.188674850254774 >100 0.11787976285189537 13.512290729181373 >500 0.022337497371729537 8.119371945514974 >1k 0.006637904633677529 5.1492027519581915 >5k 1.5706124719576476E-4 0.5909355393285594 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 8832 0.18135311352618666 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 7441 0.1527908194914351 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 7047 0.14470056510632218 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT 5270 0.10821228581102849 Illumina Single End PCR Primer 1 (96% over 32bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.001560556683422802 0.0 0.0 2 0.0016426912457082124 0.0 0.0 3 0.0017453594485649757 0.0 0.0 4 0.0018890949325644443 0.0 0.0 5 0.0020122967759925604 0.0 2.0533640571352656E-5 6 0.0021765659005633813 0.0 6.160092171405796E-5 7 0.0025461714308477293 0.0 6.160092171405796E-5 8 0.0029157769611320773 0.0 6.160092171405796E-5 9 0.0033675170537018355 0.0 6.160092171405796E-5 10-11 0.005595417055693599 0.0 6.160092171405796E-5 12-13 0.0075666465505434535 0.0 6.160092171405796E-5 14-15 0.009722678810535483 0.0 6.160092171405796E-5 16-17 0.011868444250241835 0.0 6.160092171405796E-5 18-19 0.014250346556518743 0.0 6.160092171405796E-5 20-21 0.017494661766792464 0.0 6.160092171405796E-5 22-23 0.02097511384363674 0.0 6.160092171405796E-5 24-25 0.024917572833336447 0.0 6.160092171405796E-5 26-27 0.029896980671889464 0.0 6.160092171405796E-5 28-29 0.03503039081472763 0.0 6.160092171405796E-5 30-31 0.054496282076369945 0.0 6.160092171405796E-5 32-33 0.06189865950234258 0.0 6.160092171405796E-5 34-35 0.06863369360974625 0.0 6.160092171405796E-5 36-37 0.07561513140400615 0.0 6.160092171405796E-5 38-39 0.08380805399197586 0.0 6.160092171405796E-5 40-41 0.09266831989851454 0.0 6.160092171405796E-5 42-43 0.1025244673727638 0.0 6.160092171405796E-5 44-45 0.11329436185243827 0.0 6.160092171405796E-5 46-47 0.12452626324496818 0.0 6.160092171405796E-5 48-49 0.13616883744892513 0.0 6.160092171405796E-5 50-51 0.14917689875087703 0.0 6.160092171405796E-5 52-53 0.16394058632167963 0.0 6.160092171405796E-5 54-55 0.17970015546019275 0.0 6.160092171405796E-5 56-57 0.19661987529098734 0.0 6.160092171405796E-5 58-59 0.21440200802577875 0.0 6.160092171405796E-5 60-61 0.2339911011308492 0.0 6.160092171405796E-5 62-63 0.254997015435343 0.0 6.160092171405796E-5 64-65 0.2775942868841166 0.0 8.213456228541063E-5 66-67 0.303877346815448 0.0 8.213456228541063E-5 68-69 0.331248689697061 0.0 8.213456228541063E-5 70-71 0.3609814012443797 0.0 8.213456228541063E-5 72-73 0.39292147915311876 0.0 8.213456228541063E-5 74-75 0.4274179953129912 0.0 9.240138257108695E-5 76-77 0.4664421792188469 0.0 1.3346866371379226E-4 78-79 0.5098400285664008 0.0 1.437354839994686E-4 80-81 0.5576628774570811 0.0 1.540023042851449E-4 82-83 0.6091201807288908 0.0 1.6426912457082125E-4 84-85 0.6661215669549658 0.0 1.6426912457082125E-4 86-87 0.7260900642436012 0.0 1.6426912457082125E-4 88-89 0.7939126790507791 0.0 1.848027651421739E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 1580 0.0 74.431015 9 CGGGAGA 2130 0.0 73.06252 4 AGAGCGG 1785 0.0 69.0715 8 GAGAGCG 2165 0.0 63.303913 7 GATCCGG 455 0.0 61.546295 1 CGGAAAG 1080 0.0 60.18563 5 TCGGGAG 2765 0.0 55.7742 3 AAGCGGC 710 0.0 55.434364 9 ATTCGGA 415 0.0 54.8887 2 TCGGGAA 875 0.0 54.229683 3 TTGTGTT 66020 0.0 50.97204 1 GATCGGG 3915 0.0 49.70652 1 TCGGAAA 1030 0.0 46.98545 4 GGATCGG 995 0.0 46.74813 1 ATCGGGA 4160 0.0 45.174324 2 AAGCGTC 280 0.0 44.032635 9 TCGGAGA 550 0.0 43.132812 4 GTTCGGA 455 0.0 42.76242 2 GGTTCGG 675 0.0 42.189926 1 ACCGGAA 405 0.0 42.17864 3 >>END_MODULE