FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n02_34344-1.34200000035854.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n02_34344-1.34200000035854.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4870057
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC88320.18135311352618666No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG74410.1527908194914351No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA70470.14470056510632218No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT52700.10821228581102849Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC15800.074.4310159
CGGGAGA21300.073.062524
AGAGCGG17850.069.07158
GAGAGCG21650.063.3039137
GATCCGG4550.061.5462951
CGGAAAG10800.060.185635
TCGGGAG27650.055.77423
AAGCGGC7100.055.4343649
ATTCGGA4150.054.88872
TCGGGAA8750.054.2296833
TTGTGTT660200.050.972041
GATCGGG39150.049.706521
TCGGAAA10300.046.985454
GGATCGG9950.046.748131
ATCGGGA41600.045.1743242
AAGCGTC2800.044.0326359
TCGGAGA5500.043.1328124
GTTCGGA4550.042.762422
GGTTCGG6750.042.1899261
ACCGGAA4050.042.178643
Produced by FastQC (version 0.11.2)