##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n02_34311-1.34200000035761.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2663197 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.937737613852825 33.0 33.0 33.0 27.0 33.0 2 31.89898907215651 33.0 33.0 33.0 33.0 33.0 3 31.87955265795208 33.0 33.0 33.0 33.0 33.0 4 35.61418926200353 37.0 37.0 37.0 33.0 37.0 5 35.62931018621604 37.0 37.0 37.0 33.0 37.0 6 35.627887460071484 37.0 37.0 37.0 33.0 37.0 7 35.549697975778734 37.0 37.0 37.0 33.0 37.0 8 35.49930515842426 37.0 37.0 37.0 33.0 37.0 9 35.46100795397412 37.0 37.0 37.0 33.0 37.0 10-11 35.38036465195778 37.0 37.0 37.0 33.0 37.0 12-13 35.37823093822951 37.0 37.0 37.0 33.0 37.0 14-15 37.03401381872989 40.0 37.0 40.0 33.0 40.0 16-17 36.99851775891908 40.0 37.0 40.0 33.0 40.0 18-19 36.97859245861271 40.0 37.0 40.0 33.0 40.0 20-21 36.897773615695726 40.0 37.0 40.0 33.0 40.0 22-23 36.945439447401 40.0 37.0 40.0 33.0 40.0 24-25 36.739155796585834 40.0 37.0 40.0 33.0 40.0 26-27 36.6549921015982 40.0 37.0 40.0 33.0 40.0 28-29 36.667715906859314 40.0 37.0 40.0 33.0 40.0 30-31 36.635918972573194 40.0 37.0 40.0 33.0 40.0 32-33 36.53330151693622 40.0 37.0 40.0 33.0 40.0 34-35 36.488724829593906 40.0 37.0 40.0 33.0 40.0 36-37 36.4062042725341 40.0 37.0 40.0 33.0 40.0 38-39 36.36494258592211 40.0 37.0 40.0 33.0 40.0 40-41 36.258037050957924 37.0 37.0 40.0 33.0 40.0 42-43 36.164081928599344 37.0 37.0 40.0 33.0 40.0 44-45 36.02950870701642 37.0 37.0 40.0 30.0 40.0 46-47 35.96464399742114 37.0 37.0 40.0 27.0 40.0 48-49 35.84843930058497 37.0 37.0 40.0 27.0 40.0 50-51 34.64787340177989 37.0 35.0 38.5 27.0 38.5 52-53 35.105801598604984 37.0 35.0 38.5 27.0 40.0 54-55 35.93185915274011 37.0 37.0 40.0 27.0 40.0 56-57 35.97006849286778 37.0 37.0 40.0 27.0 40.0 58-59 35.74739758267977 37.0 37.0 40.0 27.0 40.0 60-61 35.48995943597113 37.0 37.0 40.0 27.0 40.0 62-63 35.26622758286375 37.0 37.0 40.0 27.0 40.0 64-65 35.02601722666404 37.0 33.0 40.0 27.0 40.0 66-67 34.803910300289466 37.0 33.0 37.0 27.0 40.0 68-69 34.58294598559551 37.0 33.0 37.0 27.0 40.0 70-71 34.20116912117278 37.0 33.0 37.0 27.0 40.0 72-73 33.95463009307986 37.0 33.0 37.0 27.0 38.5 74-75 33.70585916100086 37.0 33.0 37.0 27.0 37.0 76-77 33.46226997101604 37.0 33.0 37.0 27.0 37.0 78-79 33.24012943841556 37.0 33.0 37.0 27.0 37.0 80-81 33.05119204474923 37.0 33.0 37.0 27.0 37.0 82-83 32.895340074354245 37.0 33.0 37.0 27.0 37.0 84-85 32.74397331477919 37.0 33.0 37.0 27.0 37.0 86-87 32.62951032161722 37.0 33.0 37.0 27.0 37.0 88-89 32.51213916957701 35.0 33.0 37.0 24.5 37.0 90-91 32.378131246017475 33.0 33.0 37.0 22.0 37.0 92-93 32.25328073739945 33.0 33.0 37.0 22.0 37.0 94-95 32.14440651592804 33.0 33.0 37.0 22.0 37.0 96-97 32.01277374523927 33.0 33.0 37.0 22.0 37.0 98-99 31.87455715818244 33.0 33.0 37.0 22.0 37.0 100-101 30.641239082200826 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.3427487227182091 2306 2 0.2256867266635716 2306 3 0.2106920125376739 2306 4 0.3369202709946606 2306 5 0.3086782562770196 2306 6 0.31065785939303225 2306 7 0.3257461867410143 2306 8 0.288081568002184 2306 9 0.22747604911909747 2306 10-11 0.2724400026961078 2306 12-13 0.37272354152130305 2306 14-15 0.5868255135261933 2306 16-17 0.669301348841195 2306 18-19 0.6537083308908436 2306 20-21 0.8043191612339129 2306 22-23 0.7199394122111471 2306 24-25 0.5809108946086141 2306 26-27 0.6721385807389808 2306 28-29 0.5283680276432676 2306 30-31 0.581898334410262 2306 32-33 0.38652293756616274 2306 34-35 0.45306235722984667 2306 36-37 0.4592304918578094 2306 38-39 0.26734663099110634 2306 40-41 0.28744317592300206 2306 42-43 0.2597046425808145 2306 44-45 0.317595501361545 2306 46-47 0.3418048903963111 2306 48-49 0.2671079933536973 2306 50-51 0.38243066103751744 2306 52-53 0.2760748585744466 2306 54-55 0.5964699437969685 2306 56-57 0.4741231792741729 2306 58-59 0.5352807806000399 2306 60-61 0.748737581190916 2306 62-63 0.7284465218740479 2306 64-65 0.5498047528738965 2306 66-67 0.4289347933561771 2306 68-69 0.39633337397258117 2306 70-71 0.5308764704234079 2306 72-73 0.5302787030152913 2306 74-75 0.5772444991351051 2306 76-77 0.5636724971403595 2306 78-79 0.6307291623857125 2306 80-81 0.4854712741989857 2306 82-83 0.4607354561699921 2306 84-85 0.5253134012864962 2306 86-87 0.6265097971694544 2306 88-89 0.6424029856748632 2306 90-91 0.599813978234188 2306 92-93 0.455748167712521 2306 94-95 0.4062134901527301 2306 96-97 0.5655511992331057 2306 98-99 0.3417913937757966 2306 100-101 0.1975957568889477 2307 1 0.2843571872192463 2307 2 0.24543434978098233 2307 3 0.2508766315512396 2307 4 0.371142230756881 2307 5 0.26566887000982575 2307 6 0.35047785834651535 2307 7 0.4074061299466649 2307 8 0.378533921130483 2307 9 0.2688276184388485 2307 10-11 0.40927053244171674 2307 12-13 0.4649466690644033 2307 14-15 0.5694969244064865 2307 16-17 0.4814157049877821 2307 18-19 0.6194988296728141 2307 20-21 0.665342854893261 2307 22-23 0.4219256363835484 2307 24-25 0.5128079239102803 2307 26-27 0.5698908932524276 2307 28-29 0.46124595965782333 2307 30-31 0.3827144521119479 2307 32-33 0.3429566543734879 2307 34-35 0.33874192513430756 2307 36-37 0.4104312048187637 2307 38-39 0.23535516592445305 2307 40-41 0.1961079272961399 2307 42-43 0.15479290306073779 2307 44-45 0.30131010767569677 2307 46-47 0.2977435877143151 2307 48-49 0.3380108142172702 2307 50-51 0.22049450360138678 2307 52-53 0.35815174772579894 2307 54-55 0.46679850883015916 2307 56-57 0.43048297538594227 2307 58-59 0.2987789903072411 2307 60-61 0.28221410472551867 2307 62-63 0.4424382145167769 2307 64-65 0.4425001422989183 2307 66-67 0.30248098506991994 2307 68-69 0.38133577847160893 2307 70-71 0.19595799498376465 2307 72-73 0.35039393624338544 2307 74-75 0.4237286564350313 2307 76-77 0.46159632227887926 2307 78-79 0.3531947415045238 2307 80-81 0.3329247062372147 2307 82-83 0.26198803820327043 2307 84-85 0.207788714681179 2307 86-87 0.38889670473452753 2307 88-89 0.46046671195898625 2307 90-91 0.46974096285951106 2307 92-93 0.2184789310973585 2307 94-95 0.16765313307423924 2307 96-97 0.21838722070499017 2307 98-99 0.2678557479705468 2307 100-101 0.16802913980728462 2201 1 0.1922667421421842 2201 2 0.19317803020502922 2201 3 0.2175704701998349 2201 4 0.28565165107400503 2201 5 0.21878424224507853 2201 6 0.2389565199800927 2201 7 0.2377267175303217 2201 8 0.18129041418908542 2201 9 0.2792114958388652 2201 10-11 0.22589393295255178 2201 12-13 0.2260205057900535 2201 14-15 0.26926115520947747 2201 16-17 0.25396029839042455 2201 18-19 0.3021108513936852 2201 20-21 0.35021474944925046 2201 22-23 0.38074043633505994 2201 24-25 0.47281787303596445 2201 26-27 0.32696585029979275 2201 28-29 0.3156275462507452 2201 30-31 0.24613134974045892 2201 32-33 0.17273254822718087 2201 34-35 0.32642925504815423 2201 36-37 0.22740575094216098 2201 38-39 0.3246247412472698 2201 40-41 0.2821569957151979 2201 42-43 0.13300097355739382 2201 44-45 0.3143865194691742 2201 46-47 0.4478813287611487 2201 48-49 0.41243361861662464 2201 50-51 0.30128015010644305 2201 52-53 0.42671878100520644 2201 54-55 0.414755802835181 2201 56-57 0.5007359256361354 2201 58-59 0.43573539390731497 2201 60-61 0.4866799244624147 2201 62-63 0.43147411079505105 2201 64-65 0.46368425546813086 2201 66-67 0.45293475716805176 2201 68-69 0.4142573517711625 2201 70-71 0.3778355371316948 2201 72-73 0.477854777237134 2201 74-75 0.43423627086042416 2201 76-77 0.4089972177001897 2201 78-79 0.514726321617978 2201 80-81 0.339645800473825 2201 82-83 0.4762031650546277 2201 84-85 0.4710542852718973 2201 86-87 0.40609155292676036 2201 88-89 0.4937941928654439 2201 90-91 0.555038412455481 2201 92-93 0.35964155749932303 2201 94-95 0.27796594529076657 2201 96-97 0.20050847462924537 2201 98-99 0.3588898299964889 2201 100-101 0.22096559499232526 1101 1 0.3609040604194682 1101 2 0.387016504961597 1101 3 0.35283354196155514 1101 4 0.4422891330040102 1101 5 0.4836957333941925 1101 6 0.39838973602896743 1101 7 0.46938397416507627 1101 8 0.4130158594608915 1101 9 0.4407735611363037 1101 10-11 0.5021370118058925 1101 12-13 0.41304284438755445 1101 14-15 0.6414545109086873 1101 16-17 0.7050642724241882 1101 18-19 0.6758139035170743 1101 20-21 0.8040305855783174 1101 22-23 0.5999396289815948 1101 24-25 0.6666140708375607 1101 26-27 0.6483259408543063 1101 28-29 0.5782206237403145 1101 30-31 0.45683628387023845 1101 32-33 0.5226493580899856 1101 34-35 0.6139098212865974 1101 36-37 0.38905918901323844 1101 38-39 0.4326628177821945 1101 40-41 0.4413508696480406 1101 42-43 0.4958293018447648 1101 44-45 0.3548517023510982 1101 46-47 0.48421223457900453 1101 48-49 0.492877799398336 1101 50-51 0.573772172035909 1101 52-53 0.6054555183152956 1101 54-55 0.679083599251193 1101 56-57 0.6444898464827702 1101 58-59 0.6164380096234368 1101 60-61 0.7743971052713974 1101 62-63 0.630177162918443 1101 64-65 0.6541603485319811 1101 66-67 0.7508379565789056 1101 68-69 0.7039785966229246 1101 70-71 0.5969644954820694 1101 72-73 0.5529618333972905 1101 74-75 0.5701103128100726 1101 76-77 0.618209502565044 1101 78-79 0.6786340760297875 1101 80-81 0.6469372114331051 1101 82-83 0.645831946114555 1101 84-85 0.6878627843036114 1101 86-87 0.801815443497631 1101 88-89 0.721050554865279 1101 90-91 0.837299820414465 1101 92-93 0.7634901241741758 1101 94-95 0.7454038254424162 1101 96-97 0.7436098302193486 1101 98-99 0.7950918592903271 1101 100-101 0.6153829912774107 2304 1 0.1988136223105279 2304 2 0.1261967012266858 2304 3 0.10958832466180368 2304 4 0.19154272030337438 2304 5 0.21725096530196453 2304 6 0.28670683091966964 2304 7 0.2863426549689123 2304 8 0.25858651533019383 2304 9 0.1716892601497193 2304 10-11 0.30466862961100105 2304 12-13 0.2573928753144372 2304 14-15 0.41429204167106803 2304 16-17 0.4410825785082366 2304 18-19 0.35590345988077843 2304 20-21 0.5146569317751926 2304 22-23 0.4790163846855222 2304 24-25 0.30049436405075625 2304 26-27 0.3349305417220094 2304 28-29 0.30175115800773966 2304 30-31 0.2031264833376767 2304 32-33 0.1142850613147175 2304 34-35 0.0415575794164198 2304 36-37 0.21013948047171738 2304 38-39 0.13303481776464565 2304 40-41 0.2770901757452435 2304 42-43 0.11341452625113391 2304 44-45 0.19975655282728866 2304 46-47 0.26170755167827764 2304 48-49 0.23755607205502116 2304 50-51 0.21077640967721578 2304 52-53 0.23590172088859163 2304 54-55 0.276975087558192 2304 56-57 0.42945038728650786 2304 58-59 0.40281131686997895 2304 60-61 0.46428543947478573 2304 62-63 0.35819538565343834 2304 64-65 0.45095459042848063 2304 66-67 0.27671312163197115 2304 68-69 0.41646450405283275 2304 70-71 0.527472113146807 2304 72-73 0.5488968123626208 2304 74-75 0.4199107137628886 2304 76-77 0.4487825931040419 2304 78-79 0.4411123065732667 2304 80-81 0.3139859311477835 2304 82-83 0.2858065904553655 2304 84-85 0.3141749835341727 2304 86-87 0.43700376848875067 2304 88-89 0.43175213454916417 2304 90-91 0.30307384797229275 2304 92-93 0.398681410250056 2304 94-95 0.3375307397288836 2304 96-97 0.4759261223270741 2304 98-99 0.3911388111277354 2304 100-101 0.2938891349799526 2202 1 0.290755122448747 2202 2 0.18237065576129297 2202 3 0.25878682959801225 2202 4 0.19856399125907132 2202 5 0.3277974512582844 2202 6 0.26104100115548334 2202 7 0.34510116126840273 2202 8 0.379987171976758 2202 9 0.09266067292440994 2202 10-11 0.27984074371754275 2202 12-13 0.3391217984367998 2202 14-15 0.43937487986866586 2202 16-17 0.4535241184087937 2202 18-19 0.5206476176577226 2202 20-21 0.5852610163136944 2202 22-23 0.44109220577773556 2202 24-25 0.28639066388148393 2202 26-27 0.45707816223028885 2202 28-29 0.45344276133868533 2202 30-31 0.3604581845218391 2202 32-33 0.25052445329180983 2202 34-35 0.03217376306539421 2202 36-37 0.25349178611910617 2202 38-39 0.24911792483135997 2202 40-41 0.24552494090131916 2202 42-43 0.08517304391128278 2202 44-45 0.1435523031804138 2202 46-47 0.2340683740391043 2202 48-49 0.2829746937031601 2202 50-51 0.1087958667130664 2202 52-53 0.289258513999485 2202 54-55 0.4166312001651207 2202 56-57 0.3066375997196218 2202 58-59 0.24429829565203676 2202 60-61 0.3917957682146209 2202 62-63 0.3788570309052446 2202 64-65 0.4065128914785916 2202 66-67 0.5188120616817287 2202 68-69 0.4772120738167942 2202 70-71 0.4879330154109809 2202 72-73 0.49208978465395603 2202 74-75 0.534589453031785 2202 76-77 0.532240136397661 2202 78-79 0.4829964353426348 2202 80-81 0.4086681922234874 2202 82-83 0.3857744018985869 2202 84-85 0.39226110875144826 2202 86-87 0.3820769311848622 2202 88-89 0.4207649493816561 2202 90-91 0.4939131740575178 2202 92-93 0.45383877442381504 2202 94-95 0.4393384112086878 2202 96-97 0.4778359451385299 2202 98-99 0.44838795813098287 2202 100-101 0.17124107708599112 1102 1 0.17344623642477686 1102 2 0.12314050044175318 1102 3 0.11851629938334796 1102 4 0.1337970387826175 1102 5 0.12309148063052788 1102 6 0.12901563980051378 1102 7 0.20712665904808603 1102 8 0.184856511203634 1102 9 0.22205610678911825 1102 10-11 0.20179881358063767 1102 12-13 0.1565297629118092 1102 14-15 0.21280821251987447 1102 16-17 0.2897815450293493 1102 18-19 0.25075740544907177 1102 20-21 0.37704347345928113 1102 22-23 0.2047834493572509 1102 24-25 0.04091416644967438 1102 26-27 0.0458149375592356 1102 28-29 -0.03628752699494697 1102 30-31 -0.029527729626089183 1102 32-33 -0.07270594759221893 1102 34-35 -0.016452951622774492 1102 36-37 -0.028354858856033616 1102 38-39 0.0017113587097128402 1102 40-41 0.005107754115414309 1102 42-43 -0.12591316968889998 1102 44-45 0.03353395405591897 1102 46-47 -0.03060171688796487 1102 48-49 -0.0771531362570741 1102 50-51 -0.12557770509067012 1102 52-53 0.060548443808968955 1102 54-55 0.23443155623363765 1102 56-57 0.2330634085476646 1102 58-59 0.10980995655476278 1102 60-61 0.2560342454419171 1102 62-63 0.2089714230308104 1102 64-65 0.3290548591322846 1102 66-67 0.24440448601716724 1102 68-69 0.1670422932559532 1102 70-71 0.28762196705524445 1102 72-73 0.3748005096831193 1102 74-75 0.3245574614355462 1102 76-77 0.2573907151795254 1102 78-79 0.2950734334531546 1102 80-81 0.1055539569863484 1102 82-83 0.22266773823326957 1102 84-85 0.18515048194807804 1102 86-87 0.28134279567419185 1102 88-89 0.2786675216696963 1102 90-91 0.283397897899782 1102 92-93 0.27984848786369554 1102 94-95 0.41721543796838745 1102 96-97 0.19276848311576344 1102 98-99 0.0638798374496723 1102 100-101 0.0857928195706954 2305 1 0.1464070009103473 2305 2 0.10676575121003751 2305 3 0.11786806741332967 2305 4 0.19116395101456618 2305 5 0.12111983280914984 2305 6 0.19015674368303337 2305 7 0.1513308122298156 2305 8 0.15695527655967823 2305 9 -0.041000554137049505 2305 10-11 0.09746165040638033 2305 12-13 0.10536317638215564 2305 14-15 0.0033247127880002836 2305 16-17 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1213 72-73 -1.4542652404570546 1213 74-75 -1.3226324136934835 1213 76-77 -1.2805369627041188 1213 78-79 -1.199944019995094 1213 80-81 -1.0967335301053822 1213 82-83 -1.1328728136984623 1213 84-85 -1.0945119807756143 1213 86-87 -1.002628458795293 1213 88-89 -1.1533828274696774 1213 90-91 -1.1090597500018688 1213 92-93 -1.1994134840591002 1213 94-95 -1.4036283509035314 1213 96-97 -1.2110548289614655 1213 98-99 -1.1484202717311973 1213 100-101 -0.979926891992239 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 269.0 3 108.0 4 102.0 5 146.0 6 527.0 7 1677.0 8 5007.0 9 10589.0 10 11685.0 11 11373.0 12 8100.0 13 6430.0 14 6263.0 15 6551.0 16 6892.0 17 7139.0 18 7574.0 19 8232.0 20 9157.0 21 10267.0 22 11282.0 23 12405.0 24 14057.0 25 16073.0 26 18797.0 27 22408.0 28 26889.0 29 33264.0 30 41457.0 31 53707.0 32 73000.0 33 105334.0 34 162161.0 35 276333.0 36 538235.0 37 907749.0 38 231812.0 39 146.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.59242141187393 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21.51052377914673 28.177403398537574 31.773517261853872 18.53855556046182 20-21 21.60615680854278 28.329620518786918 31.381550318031877 18.682672354638424 22-23 21.66586700509923 27.37026360265672 32.10831946846092 18.855549923783137 24-25 22.079034560174776 27.389855096516825 31.681832446672075 18.84927789663632 26-27 21.7091993359854 27.875113742718632 31.86166776714453 18.554019154151437 28-29 20.966077996508808 28.468248242279508 31.16484205611096 19.400831705100728 30-31 20.779251573876355 28.792607763459273 30.586091925708033 19.842048736956347 32-33 19.991427009475846 28.95912524623346 31.89830181745893 19.151145926831767 34-35 20.268249713907124 29.178066463892065 32.089193218093115 18.46449060410769 36-37 20.250711442043475 30.204519235710126 31.468127641144473 18.076641681101925 38-39 20.73758051331983 29.910648899612074 30.36043535835134 18.991335228716753 40-41 21.16384117400331 29.095558706566194 29.88034735750609 19.860252761924414 42-43 20.870907429365975 29.3099510084442 30.95090544085527 18.868236121334554 44-45 20.186081834815614 29.498513578107698 31.83119157174031 18.48421301533638 46-47 20.43413748691336 29.228867431403415 31.557197086669326 18.779797995013897 48-49 20.786203253276913 30.314541923014577 29.726021904014754 19.17323291969375 50-51 20.462979787471415 29.82732405478255 29.872232416305216 19.837463741440814 52-53 20.41136389270134 29.489979044671248 30.102332577204166 19.99632448542325 54-55 21.4532966422708 29.331082121540586 29.780602857399952 19.43501837878866 56-57 21.782521038528337 29.405890336059144 28.98092110118828 19.830667524224243 58-59 21.135135236897543 29.252172996296917 30.162186381615236 19.450505385190304 60-61 20.14560534856202 29.639690660625785 30.306190570565857 19.908513420246337 62-63 20.869445588276257 29.69059705009331 29.700157560218038 19.739799801412396 64-65 20.702443379508974 29.71730781101561 29.612671126146672 19.96757768332874 66-67 21.117604898974463 29.33290320249225 29.44697947267063 20.10251242586266 68-69 21.97231426853221 29.25650945962098 29.326246107311622 19.444930164535197 70-71 21.16718523959462 30.608995259541473 29.193444545630935 19.030374955232976 72-73 20.26243488995273 31.23609354770722 28.936429259627257 19.565042302712794 74-75 21.25687716684627 30.17428115563127 28.705276537668073 19.863565139854387 76-77 21.528027270621166 29.329373901512678 29.150491845669485 19.992106982196674 78-79 21.479993842000642 29.280515314635753 29.7393728401363 19.500118003227303 80-81 19.816022777128538 30.074539120569 30.01767911419601 20.091758988106452 82-83 19.144672905360398 29.78233226936909 30.302083258810654 20.77091156645986 84-85 19.094018735221916 29.04433801713881 30.81605144655793 21.045591801081343 86-87 19.126735135511648 29.18103768798576 31.92743469621539 19.764792480287205 88-89 19.538850235512733 29.785051920858084 31.22821100118655 19.447886842442628 90-91 19.91117576348975 29.54586096149582 30.317360104839253 20.225603170175173 92-93 19.03781446185809 30.627982777050118 30.42948225765172 19.904720503440075 94-95 18.80888568053056 30.914801149739795 30.741971336295883 19.534341833433764 96-97 19.497409934830618 31.62429301984588 29.78096975773369 19.097327287589817 98-99 20.08112505964386 30.554765532578486 29.064454895125625 20.299654512652026 100-101 19.574342965225195 30.562620850425574 28.67048343182078 21.192552752528446 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 814.0 1 595.5 2 371.0 3 462.0 4 514.5 5 501.5 6 621.5 7 853.5 8 1047.0 9 1189.5 10 1322.5 11 1378.0 12 1392.5 13 1334.5 14 1186.0 15 1101.0 16 1037.5 17 955.5 18 987.5 19 1099.0 20 1251.0 21 1478.0 22 1779.5 23 2154.5 24 2680.0 25 3483.5 26 4917.0 27 7157.5 28 9460.0 29 11374.0 30 13737.0 31 17300.5 32 24486.5 33 35813.0 34 52534.5 35 80000.5 36 125798.5 37 185883.0 38 235442.0 39 243162.0 40 219413.5 41 189205.0 42 164030.5 43 153928.0 44 137667.5 45 118349.5 46 114185.5 47 102177.0 48 77401.5 49 59461.0 50 48410.0 51 40985.5 52 35252.0 53 29321.0 54 23547.5 55 17377.5 56 12334.0 57 9409.0 58 7599.5 59 5899.5 60 4595.0 61 3551.0 62 2690.5 63 2024.0 64 1557.5 65 1128.5 66 761.0 67 585.0 68 466.5 69 349.5 70 252.0 71 203.0 72 161.0 73 113.0 74 91.5 75 80.5 76 60.5 77 48.5 78 44.5 79 43.5 80 35.0 81 23.5 82 20.0 83 17.5 84 13.0 85 10.5 86 10.0 87 9.5 88 9.0 89 8.0 90 7.5 91 6.0 92 3.5 93 4.0 94 3.0 95 2.5 96 3.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1866553619578274 2 0.17794402742268034 3 0.1502705207312865 4 0.14377456868568114 5 0.14843062679929425 6 0.12830444011464417 7 0.1030716090473217 8 0.08977931411007146 9 0.08974176525431651 10-11 0.07027268354537798 12-13 0.08902833699497259 14-15 0.09321503441164886 16-17 0.0857991354000474 18-19 0.08258870823299966 20-21 0.08046719788284531 22-23 0.1012504895432069 24-25 0.4525763584143419 26-27 0.28283675597411684 28-29 0.12616415533661235 30-31 0.23661411453978057 32-33 0.26963833317625396 34-35 0.25221566410595986 36-37 0.2552007981384779 38-39 0.272980181338444 40-41 0.2725671439251396 42-43 0.29826933568939884 44-45 0.2749514962655786 46-47 0.29351940543639843 48-49 0.2772044276108752 50-51 0.29248681190313747 52-53 0.2923741653358726 54-55 0.2683241232248309 56-57 0.28510846174729093 58-59 0.2741817447226022 60-61 0.24904278579466707 62-63 0.24162688678306563 64-65 0.23974944399531842 66-67 0.2493619510685841 68-69 0.25437472331186917 70-71 0.23980576727895084 72-73 0.23118830488319114 74-75 0.24023757912013266 76-77 0.24100733066310903 78-79 0.24369207384958755 80-81 0.2505447400248649 82-83 0.27808682572111637 84-85 0.27174106909853085 86-87 0.2720226855166929 88-89 0.2697322053156413 90-91 0.2605890589393124 92-93 0.23569416757378445 94-95 0.23460525075689104 96-97 0.22983654607601317 98-99 0.25550118898451746 100-101 0.2636305162554629 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2663197.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.460331235169924 #Duplication Level Percentage of deduplicated Percentage of total 1 86.435556506925 35.83646803273357 2 6.07180262471378 5.034778960304149 3 2.103325962811159 2.6161377334105005 4 1.2326767298693033 2.0442874210506954 5 0.6484863137541363 1.344322868486041 6 0.5117770003013341 1.273106637062098 7 0.42856423984373604 1.2437890737629071 8 0.2897131506998838 0.9609282552961504 9 0.19278248384926588 0.7193543073056423 >10 1.6750993528245082 13.649684169802564 >50 0.19135353375358777 5.5399190820702735 >100 0.18649887410013388 16.953014473094346 >500 0.02556731419815194 7.053940308203755 >1k 0.006340146360918092 4.669511081892712 >5k 4.5576599529278874E-4 1.0607575955245852 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 6137 0.23043732776809228 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 5846 0.21951061074340353 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 5761 0.21631895800423326 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 5164 0.1939022911185316 No Hit TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT 5160 0.19375209569551183 No Hit GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT 4646 0.17445198383747054 No Hit TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA 4273 0.16044626064087636 No Hit TGTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAG 3969 0.14903140849137334 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 3953 0.14843062679929425 No Hit GTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGT 3894 0.14621524430975252 No Hit TTGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGG 3333 0.12515033623122887 No Hit TGTGTTTGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAA 3019 0.11335999552417639 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 2905 0.10907942596811276 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 2896 0.10874148626631827 No Hit GTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAAT 2838 0.1065636526325315 No Hit TTGTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTA 2693 0.10111906854806459 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 3.754885575494415E-4 0.0 0.0 2 3.754885575494415E-4 0.0 0.0 3 3.754885575494415E-4 0.0 0.0 4 4.1303741330438567E-4 0.0 0.0 5 4.88135124814274E-4 0.0 3.7548855754944155E-5 6 6.007816920791065E-4 0.0 3.7548855754944155E-5 7 6.758794035889947E-4 0.0 3.7548855754944155E-5 8 9.387213938736038E-4 0.0 3.7548855754944155E-5 9 0.001013819105383492 0.0 3.7548855754944155E-5 10-11 0.0015770519417076545 0.0 3.7548855754944155E-5 12-13 0.0023468034846840097 0.0 3.7548855754944155E-5 14-15 0.0030977805997828923 0.0 3.7548855754944155E-5 16-17 0.003717336719739471 0.0 3.7548855754944155E-5 18-19 0.004449539406960882 0.0 3.7548855754944155E-5 20-21 0.005294388661447125 0.0 3.7548855754944155E-5 22-23 0.006214335627443257 0.0 3.7548855754944155E-5 24-25 0.007528545578866302 0.0 3.7548855754944155E-5 26-27 0.009068048664819013 0.0 3.7548855754944155E-5 28-29 0.011245882298605774 0.0 3.7548855754944155E-5 30-31 0.01826751832478033 0.0 3.7548855754944155E-5 32-33 0.020163735540405008 0.0 3.7548855754944155E-5 34-35 0.022285245890559354 0.0 3.7548855754944155E-5 36-37 0.02386229783226701 0.0 3.7548855754944155E-5 38-39 0.026246650172705964 0.0 3.7548855754944155E-5 40-41 0.02859345365738997 0.0 3.7548855754944155E-5 42-43 0.030808836146931674 0.0 3.7548855754944155E-5 44-45 0.03366254918430743 0.0 3.7548855754944155E-5 46-47 0.03676032978409033 0.0 3.7548855754944155E-5 48-49 0.04004585466264794 0.0 3.7548855754944155E-5 50-51 0.04370686809875499 0.0 3.7548855754944155E-5 52-53 0.048456798351755426 0.0 3.7548855754944155E-5 54-55 0.05303775875385862 0.0 3.7548855754944155E-5 56-57 0.058388470698938155 0.0 3.7548855754944155E-5 58-59 0.06464035518213636 0.0 3.7548855754944155E-5 60-61 0.07156811906892355 0.0 3.7548855754944155E-5 62-63 0.07928440892656458 0.0 3.7548855754944155E-5 64-65 0.08812716445685392 0.0 3.7548855754944155E-5 66-67 0.09753315282346743 0.0 5.632328363241623E-5 68-69 0.1088541328335831 0.0 7.509771150988831E-5 70-71 0.12135790179997949 0.0 7.509771150988831E-5 72-73 0.13618969982318244 0.0 7.509771150988831E-5 74-75 0.15336830133106938 0.0 7.509771150988831E-5 76-77 0.1723867967709486 0.0 7.509771150988831E-5 78-79 0.19600502704080847 0.0 7.509771150988831E-5 80-81 0.2218198653723326 0.0 7.509771150988831E-5 82-83 0.25351109962950547 0.0 7.509771150988831E-5 84-85 0.28961432443788426 0.0 7.509771150988831E-5 86-87 0.3311058100470975 0.0 7.509771150988831E-5 88-89 0.3799193225285249 0.0 7.509771150988831E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGAGC 40 7.5998287E-6 59.30675 1 CGGGAGA 465 0.0 53.03827 4 GAGCGGC 415 0.0 52.541653 9 AGAGCGG 425 0.0 50.190044 8 TTGTGTT 36935 0.0 48.646465 1 GAACCGG 130 0.0 47.4454 1 AACCGGA 130 0.0 47.440937 2 TCGGGAA 220 0.0 43.118393 3 CGAGGCG 100 6.4392225E-10 42.70086 1 ACCGGAA 140 0.0 40.654484 3 CGGAAAG 305 0.0 38.875046 5 TGTGTTT 63185 0.0 37.82266 1 GGTCCGG 65 1.3376745E-4 36.496464 1 CGAGGGG 265 0.0 35.80785 1 TCTCGGG 325 0.0 35.086765 48-49 GGCGCCG 325 0.0 35.07751 56-57 GGAGAGC 645 0.0 34.548573 6 CGGCGTG 90 0.0 34.23392 12-13 GCCGTAT 300 0.0 34.040314 58-59 CCGTGTA 205 0.0 33.608887 38-39 >>END_MODULE