FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n02_34310-1.34200000035810.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n02_34310-1.34200000035810.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5805783
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA283870.48894352406901875No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC239100.41183075564484584No Hit
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT141670.24401532058638775No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC114510.19723437820531703No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT106520.18347223793930983No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC88550.15252034049498575No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC75150.12943990500506133No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG73170.12602951229834114No Hit
TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT73160.12601228809275164No Hit
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT71120.12249855015249451No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA66450.11445484614219993No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC8600.054.022399
CGGGAGA11850.052.4448054
TTGTGTT711600.050.242831
CGGAAAG5850.047.0346455
AAGCGGC3950.042.0065579
AGAGCGG11500.039.9878048
TCGGGAA4750.038.953193
CGAGTGG2200.036.6699751
TCGGAAA4400.035.5803954
CGACGCC958.772422E-734.9670331
TCGGGAG19400.034.4817543
TGTGTTT1066000.033.5793762
CGAGGTG6300.030.1303271
GGCGCCG8300.029.76079856-57
ATTCGGA2400.029.6557032
GTGTTTG1509300.028.8888191
CTGACGG2300.028.885811
ACGACGC2150.028.67532316-17
TGCGGCC2500.028.4694732
CGTGGCA2850.028.3066461
Produced by FastQC (version 0.11.2)