##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n02_34302-1.342000000358e7.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8398502 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.111431895830947 33.0 33.0 33.0 27.0 33.0 2 30.76175322694452 33.0 33.0 33.0 27.0 33.0 3 30.610970504025598 33.0 33.0 33.0 27.0 33.0 4 34.09242564924078 37.0 37.0 37.0 27.0 37.0 5 34.16522827523289 37.0 37.0 37.0 27.0 37.0 6 34.53891670204996 37.0 37.0 37.0 27.0 37.0 7 34.08422883033189 37.0 37.0 37.0 27.0 37.0 8 34.309541868299846 37.0 37.0 37.0 27.0 37.0 9 33.87971628749984 37.0 37.0 37.0 27.0 37.0 10-11 33.87424578811793 37.0 37.0 37.0 24.5 37.0 12-13 33.85263645826363 37.0 37.0 37.0 24.5 37.0 14-15 35.404427837250026 40.0 37.0 40.0 24.5 40.0 16-17 35.3137040986595 40.0 37.0 40.0 24.5 40.0 18-19 35.33109505718996 40.0 37.0 40.0 22.0 40.0 20-21 35.28609786602421 40.0 37.0 40.0 22.0 40.0 22-23 35.844815480189204 40.0 37.0 40.0 27.0 40.0 24-25 35.32153394736347 40.0 37.0 40.0 22.0 40.0 26-27 35.20832012661305 38.5 37.0 40.0 24.5 40.0 28-29 35.02994450677038 38.5 37.0 40.0 22.0 40.0 30-31 35.01413496121094 40.0 37.0 40.0 18.5 40.0 32-33 34.831167272449306 37.0 37.0 40.0 15.0 40.0 34-35 34.88838926275186 37.0 37.0 40.0 18.5 40.0 36-37 34.7494012027383 37.0 37.0 40.0 18.5 40.0 38-39 34.861510778946055 37.0 37.0 40.0 22.0 40.0 40-41 34.55809416965073 37.0 37.0 40.0 15.0 40.0 42-43 34.435681327455775 37.0 37.0 40.0 15.0 40.0 44-45 34.2912124090701 37.0 35.0 40.0 15.0 40.0 46-47 34.236214863079155 37.0 33.0 40.0 15.0 40.0 48-49 34.13076218830453 37.0 33.0 40.0 15.0 40.0 50-51 33.07630074982419 37.0 33.0 38.5 15.0 38.5 52-53 33.509509255341015 37.0 33.0 38.5 18.5 40.0 54-55 34.22413782838892 37.0 33.0 40.0 18.5 40.0 56-57 34.40618023309395 37.0 33.0 40.0 18.5 40.0 58-59 33.988436211600586 37.0 33.0 40.0 15.0 40.0 60-61 33.785149661213396 37.0 33.0 40.0 15.0 40.0 62-63 33.47864756119603 37.0 33.0 40.0 15.0 40.0 64-65 33.27778322848527 37.0 33.0 38.5 15.0 40.0 66-67 33.13095478217425 37.0 33.0 37.0 15.0 40.0 68-69 33.13230180810816 37.0 33.0 37.0 18.5 40.0 70-71 32.71862309492812 37.0 33.0 37.0 15.0 40.0 72-73 32.83177898868155 37.0 33.0 37.0 22.0 37.0 74-75 32.78778453586128 37.0 33.0 37.0 22.0 37.0 76-77 32.47996874918884 37.0 33.0 37.0 22.0 37.0 78-79 32.08075517514909 37.0 33.0 37.0 22.0 37.0 80-81 31.687417768073402 37.0 33.0 37.0 15.0 37.0 82-83 31.396609538224794 35.0 33.0 37.0 15.0 37.0 84-85 31.20917819630215 33.0 33.0 37.0 15.0 37.0 86-87 31.073107918531186 33.0 33.0 37.0 15.0 37.0 88-89 30.937519452873858 33.0 33.0 37.0 15.0 37.0 90-91 30.774859850006585 33.0 33.0 37.0 15.0 37.0 92-93 30.612913767240872 33.0 30.0 37.0 15.0 37.0 94-95 30.488442284112097 33.0 27.0 37.0 15.0 37.0 96-97 30.359283357913114 33.0 27.0 37.0 15.0 37.0 98-99 30.220721742996545 33.0 27.0 37.0 15.0 37.0 100-101 29.01111400580723 33.0 27.0 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.13825420758846718 2306 2 0.1435477523359303 2306 3 0.16964524577113949 2306 4 0.36656210795969457 2306 5 0.2392837229674214 2306 6 0.036520665255714846 2306 7 0.4417006420192138 2306 8 0.09579469608615909 2306 9 0.3557955242443853 2306 10-11 0.33571871206667225 2306 12-13 0.2791763627641686 2306 14-15 0.5525378622702846 2306 16-17 0.5347829218672047 2306 18-19 0.5557010045374255 2306 20-21 0.6036299856180207 2306 22-23 0.3156146438832437 2306 24-25 0.4318935829201038 2306 26-27 0.30334569594504757 2306 28-29 0.3594509205581886 2306 30-31 0.3157613458846953 2306 32-33 0.3465659666691181 2306 34-35 0.19939392002411438 2306 36-37 0.281946468759827 2306 38-39 0.14047412822954186 2306 40-41 0.25674300640777403 2306 42-43 0.26058447530014206 2306 44-45 0.321636809857651 2306 46-47 0.3219237771131276 2306 48-49 0.26028052507616906 2306 50-51 0.1517492295756142 2306 52-53 0.23699979018957862 2306 54-55 0.48357403570999224 2306 56-57 0.32245513704182827 2306 58-59 0.4133736740544123 2306 60-61 0.4659948425574285 2306 62-63 0.4779336106659855 2306 64-65 0.46141032995981135 2306 66-67 0.2776312726338759 2306 68-69 0.19499426198267855 2306 70-71 0.3681385373120989 2306 72-73 0.04800494137950295 2306 74-75 0.06075837061482048 2306 76-77 0.23928068010011572 2306 78-79 0.36661801273066885 2306 80-81 0.29037725594186625 2306 82-83 0.26135605792965677 2306 84-85 0.36742043460256113 2306 86-87 0.3085516934897683 2306 88-89 0.3800587342083652 2306 90-91 0.3337305548027061 2306 92-93 0.3423782727344289 2306 94-95 0.3290914731678001 2306 96-97 0.31530864629947075 2306 98-99 0.20352418142573114 2306 100-101 -0.0252441996030619 2307 1 0.07835897956559279 2307 2 0.0742677909208389 2307 3 0.12633855094058788 2307 4 0.21499539499888698 2307 5 0.1314155828298169 2307 6 0.015424263995889476 2307 7 0.3055221316059402 2307 8 0.04076689054480909 2307 9 0.2252355055330284 2307 10-11 0.20502304328093857 2307 12-13 0.20273436797080535 2307 14-15 0.4516307319067536 2307 16-17 0.4146298243240878 2307 18-19 0.5170396437801372 2307 20-21 0.571112828698304 2307 22-23 0.2643251146888801 2307 24-25 0.3149347086813492 2307 26-27 0.23089947656890786 2307 28-29 0.31590434119181054 2307 30-31 0.3275603600518622 2307 32-33 0.34065910506342334 2307 34-35 0.17115782134204238 2307 36-37 0.16679608058633733 2307 38-39 0.05946597397125686 2307 40-41 0.16455016756950158 2307 42-43 0.1318215022863356 2307 44-45 0.19762049988833041 2307 46-47 0.17760371993307444 2307 48-49 0.09411626609239931 2307 50-51 -0.029694457665790708 2307 52-53 -0.01942588301249515 2307 54-55 0.19397383842721183 2307 56-57 0.15556632143585603 2307 58-59 0.21811982863810897 2307 60-61 0.19735975469540534 2307 62-63 0.21105888668756023 2307 64-65 0.1571201557466182 2307 66-67 0.048021094992201085 2307 68-69 -0.036922693296013165 2307 70-71 -0.03582030206078457 2307 72-73 -0.13969590063156545 2307 74-75 -0.045099896667032624 2307 76-77 0.08688699198706473 2307 78-79 0.19205815817951333 2307 80-81 0.18234132374439227 2307 82-83 0.003423524865180383 2307 84-85 -0.0525727157042617 2307 86-87 0.06573007025049549 2307 88-89 0.1704544910304584 2307 90-91 0.19742111573664545 2307 92-93 0.10491582619544815 2307 94-95 0.03632792341418778 2307 96-97 0.12155784728966168 2307 98-99 0.07067409164607952 2307 100-101 0.027111826093094038 2201 1 0.090683688076723 2201 2 0.047584476194856506 2201 3 0.06664189904153517 2201 4 0.17108118611876222 2201 5 0.04053200669421386 2201 6 -0.005465073716472091 2201 7 0.24603367900422057 2201 8 -0.020180213725737417 2201 9 0.08706600065411862 2201 10-11 0.059712511934655765 2201 12-13 0.1162475421324487 2201 14-15 0.3258940882982202 2201 16-17 0.314916663822288 2201 18-19 0.32128111682392557 2201 20-21 0.33785818730767403 2201 22-23 0.21110861048172325 2201 24-25 0.35721980117801166 2201 26-27 0.18530639290521123 2201 28-29 0.2394456131421805 2201 30-31 0.35292116573830157 2201 32-33 0.2683334780114919 2201 34-35 0.2298748893873963 2201 36-37 0.2183384406921789 2201 38-39 0.12799228415342156 2201 40-41 0.1841899029548486 2201 42-43 0.1990529976313482 2201 44-45 0.2684491493534438 2201 46-47 0.23534948281866974 2201 48-49 0.22982569300177857 2201 50-51 0.15994352736711193 2201 52-53 0.22097212554766799 2201 54-55 0.3636623146232054 2201 56-57 0.3805002347902615 2201 58-59 0.4683277199483271 2201 60-61 0.35396539358981727 2201 62-63 0.3893105753440125 2201 64-65 0.3679633870470198 2201 66-67 0.2889928466100926 2201 68-69 0.22308353740674391 2201 70-71 0.3216028356864342 2201 72-73 0.21118119249822342 2201 74-75 0.30884814841794395 2201 76-77 0.3861535489807508 2201 78-79 0.40937433027306014 2201 80-81 0.4061202114584965 2201 82-83 0.32785828471603295 2201 84-85 0.3240381924802911 2201 86-87 0.37368159317538385 2201 88-89 0.5272363872613646 2201 90-91 0.40080801566931257 2201 92-93 0.2913092161313955 2201 94-95 0.3720619178406537 2201 96-97 0.40080102781407234 2201 98-99 0.4444285054711834 2201 100-101 0.3277268369618369 1101 1 0.09508053581616238 1101 2 0.0032732663352845748 1101 3 0.11092496918201178 1101 4 0.1861161116346537 1101 5 0.13786769418447165 1101 6 -0.020649279444299395 1101 7 0.38371839746699976 1101 8 0.02569444690958278 1101 9 0.22180529547009797 1101 10-11 0.20220712663769547 1101 12-13 0.18704434788183733 1101 14-15 0.3050221335330505 1101 16-17 0.30654313492921403 1101 18-19 0.35286921546568095 1101 20-21 0.3919158849078741 1101 22-23 0.21182830209169623 1101 24-25 0.2556848094373194 1101 26-27 0.11917469356487942 1101 28-29 0.2645514314581874 1101 30-31 0.19528541970012725 1101 32-33 0.15172308080438768 1101 34-35 0.10113943314841123 1101 36-37 0.1306520491468106 1101 38-39 0.050681432792536896 1101 40-41 0.11115818483474271 1101 42-43 0.10862930326940301 1101 44-45 0.13346238466742477 1101 46-47 0.028961573664325613 1101 48-49 0.0947572699110566 1101 50-51 0.13298451230252084 1101 52-53 0.10331546963596594 1101 54-55 0.25358725075835054 1101 56-57 0.2033630647026854 1101 58-59 0.38538779385799415 1101 60-61 0.32558545575930253 1101 62-63 0.25500315536575613 1101 64-65 0.24949729843537938 1101 66-67 0.234350401655675 1101 68-69 0.18978533548158083 1101 70-71 0.2795724398670316 1101 72-73 0.19650360150032498 1101 74-75 0.25827864507467524 1101 76-77 0.2422715064256451 1101 78-79 0.4135334539316915 1101 80-81 0.3379998056584661 1101 82-83 0.3234444067919924 1101 84-85 0.31208619604705135 1101 86-87 0.35544615712648664 1101 88-89 0.3244907423153016 1101 90-91 0.33687919731731597 1101 92-93 0.2781306472616265 1101 94-95 0.25039342275748666 1101 96-97 0.2597642235228932 1101 98-99 0.26062850849138286 1101 100-101 0.14110731330541526 2304 1 0.11949973470710873 2304 2 0.0204188093552915 2304 3 0.1158960821252073 2304 4 0.22838917731839103 2304 5 0.17350711117229167 2304 6 0.12962961127019668 2304 7 0.34494730085158665 2304 8 0.0930547907145467 2304 9 0.18527496740095017 2304 10-11 0.28009542293396095 2304 12-13 0.21840686037202062 2304 14-15 0.4023063267336582 2304 16-17 0.3498512684384849 2304 18-19 0.39253044999140485 2304 20-21 0.5058986688080438 2304 22-23 0.3580809741409752 2304 24-25 0.4030542378034383 2304 26-27 0.3423320694319685 2304 28-29 0.27071886794545463 2304 30-31 0.3447789768264258 2304 32-33 0.2641654998844132 2304 34-35 0.13190211361891357 2304 36-37 0.2924326021195114 2304 38-39 0.21730650682366104 2304 40-41 0.2492561032561582 2304 42-43 0.2498478291130013 2304 44-45 0.3089681306959733 2304 46-47 0.29371338913924916 2304 48-49 0.28490804460264485 2304 50-51 0.23398258290382046 2304 52-53 0.27751064123452807 2304 54-55 0.47898329373254 2304 56-57 0.4697152946131382 2304 58-59 0.5023515187754839 2304 60-61 0.3534241025815632 2304 62-63 0.41217902889337665 2304 64-65 0.33910641132234787 2304 66-67 0.29623720022055977 2304 68-69 0.28895844494264367 2304 70-71 0.3766871301916197 2304 72-73 0.2854668687798778 2304 74-75 0.3807123467506415 2304 76-77 0.4293818356692185 2304 78-79 0.45274564461025335 2304 80-81 0.4676068987848474 2304 82-83 0.33835366169263636 2304 84-85 0.3117364055506222 2304 86-87 0.37950095457134125 2304 88-89 0.4577236902962376 2304 90-91 0.34898555860760183 2304 92-93 0.4636145220664467 2304 94-95 0.3907780539257715 2304 96-97 0.3860081813401095 2304 98-99 0.36461242677220085 2304 100-101 0.31247809119198067 2202 1 0.037908969346720056 2202 2 0.0050546810491418626 2202 3 0.07446355178095487 2202 4 0.11807491849690877 2202 5 0.11261838357390985 2202 6 0.044149820218514435 2202 7 0.2331416125746344 2202 8 0.06969600485096095 2202 9 0.013672393745295608 2202 10-11 0.1436538316501128 2202 12-13 0.15536729062201005 2202 14-15 0.2830034487115469 2202 16-17 0.29577248204767415 2202 18-19 0.3277086399742828 2202 20-21 0.35877120020674624 2202 22-23 0.22888544015322765 2202 24-25 0.13211359281076795 2202 26-27 0.11665765469191314 2202 28-29 0.14980784655067225 2202 30-31 0.17403881361045137 2202 32-33 0.17678386001217916 2202 34-35 -0.034458228321000206 2202 36-37 0.1297607367347382 2202 38-39 0.09651328255901603 2202 40-41 0.09277665668438573 2202 42-43 -0.002798796864418307 2202 44-45 0.09424355132757967 2202 46-47 0.14604463266660161 2202 48-49 0.10626483269389553 2202 50-51 0.03824182892717687 2202 52-53 0.1253652297117398 2202 54-55 0.21128882852095643 2202 56-57 0.226788729640802 2202 58-59 0.15670189668571766 2202 60-61 0.27826721238793795 2202 62-63 0.2068294207379182 2202 64-65 0.13393812754924994 2202 66-67 0.22525179031585907 2202 68-69 0.1827475514325343 2202 70-71 0.22117471968457636 2202 72-73 0.2163111784584757 2202 74-75 0.19638980395362893 2202 76-77 0.19145878595039534 2202 78-79 0.3374945472973465 2202 80-81 0.33793274853080035 2202 82-83 0.30315490340783313 2202 84-85 0.25671736303147696 2202 86-87 0.2833866887517651 2202 88-89 0.2667468976689591 2202 90-91 0.2650309404594573 2202 92-93 0.278436077209939 2202 94-95 0.30384363535022985 2202 96-97 0.30429298303258534 2202 98-99 0.2917176128643568 2202 100-101 0.07095497875968348 1102 1 -0.08143629952072828 1102 2 -0.0727672461767348 1102 3 -0.002427525049686352 1102 4 0.03562998501735848 1102 5 -0.07287600464952959 1102 6 -0.11052845346245732 1102 7 0.13697876152840394 1102 8 -0.1251734773855162 1102 9 -0.010757032065690453 1102 10-11 0.0966673090104635 1102 12-13 -0.02708161868075365 1102 14-15 0.045214316053481696 1102 16-17 0.0031004452651330894 1102 18-19 0.07926079156585075 1102 20-21 0.1495900155212695 1102 22-23 -0.030872073791734067 1102 24-25 -0.062395725641039235 1102 26-27 -0.17692089799194832 1102 28-29 -0.12961461003714447 1102 30-31 0.035318209957033275 1102 32-33 -0.051658503884198126 1102 34-35 -0.08426730334988264 1102 36-37 -0.037834241508505784 1102 38-39 -0.031280901020707574 1102 40-41 0.03443072923410284 1102 42-43 -0.03656778982075792 1102 44-45 0.05657395388515596 1102 46-47 0.025117928676642975 1102 48-49 -0.06592095194933023 1102 50-51 -0.08837343844303547 1102 52-53 -0.06395763897612738 1102 54-55 0.06897967106343117 1102 56-57 0.16237440332936615 1102 58-59 0.12565586971207665 1102 60-61 0.19740557251252255 1102 62-63 0.23269672226190607 1102 64-65 0.16560944232826813 1102 66-67 0.18310228970563713 1102 68-69 0.022928908376016466 1102 70-71 0.08182102924034496 1102 72-73 0.13494410624954156 1102 74-75 0.19756565864651776 1102 76-77 0.11819100159619467 1102 78-79 0.04972463747900946 1102 80-81 0.1068306899952347 1102 82-83 0.1498318196007169 1102 84-85 0.17388579070075494 1102 86-87 0.16770729979928944 1102 88-89 0.20043552622881933 1102 90-91 0.25699910553191785 1102 92-93 0.17646046808885174 1102 94-95 0.21177756646086365 1102 96-97 0.19792159192805414 1102 98-99 0.14964448257445895 1102 100-101 0.0812462295475278 2305 1 0.08572564398781068 2305 2 0.03436972461573262 2305 3 0.07017725131275654 2305 4 0.11069686057311401 2305 5 0.030848547002285898 2305 6 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-0.9850320911692876 1213 100-101 -0.8475849907855455 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 901.0 3 574.0 4 790.0 5 642.0 6 1898.0 7 10435.0 8 49051.0 9 141495.0 10 175198.0 11 124246.0 12 75197.0 13 49586.0 14 36635.0 15 29264.0 16 26776.0 17 26133.0 18 27544.0 19 28792.0 20 30852.0 21 33717.0 22 36576.0 23 39581.0 24 44064.0 25 50759.0 26 59229.0 27 70637.0 28 86036.0 29 105202.0 30 129489.0 31 166639.0 32 226402.0 33 322744.0 34 484492.0 35 820708.0 36 1616681.0 37 2602540.0 38 666717.0 39 280.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.69699642575356 23.743663472566148 25.673651569545346 13.885688532134935 2 29.635581305388836 24.865907380041275 33.58204994029394 11.916461374275949 3 36.7187794396901 20.189885553987295 28.106160088950492 14.985174917372108 4 26.78963091884307 18.194828432816298 36.97586829333323 18.039672355007408 5 33.704104767584276 17.071858444562977 33.180591195189045 16.0434455926637 6 27.266750965047965 17.62373465991612 40.258267317749656 14.851247057286256 7 33.81389657546394 19.125395956617837 31.168412912428984 15.892294555489231 8 26.737968169363597 30.202052307711007 27.489453686856642 15.570525836068756 9 32.80839991981735 26.491107805390683 23.961099493948282 16.739392780843687 10-11 26.49008429555216 29.253904828063643 27.42371011242783 16.83230076395637 12-13 28.344322290190455 24.176865079667753 27.198264108705107 20.28054852143669 14-15 25.495459150229426 24.826220129908823 28.689803944937726 20.988516774924022 16-17 25.782317209748147 26.308242642648082 29.807384919772566 18.102055227831205 18-19 25.28971388711012 26.73874659317419 29.936886170293715 18.034653349421976 20-21 24.420215341352378 28.515777728866475 28.952046967881124 18.111959961900027 22-23 26.93752424791967 26.714440407180305 28.262236893310344 18.085798451589678 24-25 24.01322717940775 29.303213861328114 28.443859839022874 18.23969912024127 26-27 22.34581854177637 30.744109348263997 28.45103293128956 18.459039178670068 28-29 23.00498325008047 29.614704164232663 28.17515885599838 19.205153729688487 30-31 24.887372489403194 27.63237659696492 28.3420028634482 19.13824805018368 32-33 22.650498503507556 27.083895800121304 29.882644979439604 20.382960716931535 34-35 20.295771753756934 27.33709155428002 33.23485494988757 19.13228174207548 36-37 21.261230312169747 28.011918202641557 30.040134976515663 20.686716508673033 38-39 20.61427192504383 27.608253010501908 27.872875128565276 23.904599935888978 40-41 22.023495848554187 27.101826884482765 28.776994306909238 22.09768296005381 42-43 23.668511720736728 27.24932018090932 30.29232191044599 18.789846187907955 44-45 21.7263944506895 29.108677041716835 30.5949306464531 18.569997861140568 46-47 23.076078929341996 29.576340470420483 28.84505836028999 18.502522239947535 48-49 21.101596158774523 28.977402600085046 30.05757265500958 19.863428586130855 50-51 20.213754460954746 28.049935348135826 30.472208475081136 21.264101715828293 52-53 23.942721244780394 27.630643233695984 28.168791327203298 20.257844194320327 54-55 25.214628402930217 27.546899739002363 27.944481668236037 19.29399018983139 56-57 25.46637737300572 27.175829241198755 27.405617226707967 19.952176159087553 58-59 24.03279542638615 26.879705531703145 28.019539290973327 21.067959750937376 60-61 21.077876438394508 27.168478097074527 27.979323344729185 23.77432211980178 62-63 23.123886535498205 27.331369542091956 28.26800749663641 21.27673642577343 64-65 21.288340896739705 28.50431811456265 29.943874625462346 20.2634663632353 66-67 20.5505900001301 28.496616575052258 28.67519633747666 22.27759708734098 68-69 20.25505656643714 27.300564798975753 32.24337647062053 20.20100216396657 70-71 19.859330557435964 31.131934054455584 29.819089673069815 19.189645715038637 72-73 19.832376350244772 34.006790702431296 27.174606307704874 18.98622663961906 74-75 20.63051901211205 33.43287267668597 26.816783477849476 19.11982483335251 76-77 20.832588884771297 32.47477378610128 27.363810669574047 19.32882665955338 78-79 20.770624351466452 32.218525997079674 27.864915967239074 19.145933684214796 80-81 20.016187795617206 32.748882135552435 27.62416526829791 19.610764800532447 82-83 19.31006318439237 32.18055729752779 28.133611382945006 20.375768135134834 84-85 19.435894797175678 31.431529114677843 28.46248178926127 20.670094298885218 86-87 19.34860257034962 31.31449519764415 29.121390607387898 20.215511624618333 88-89 19.61550271249596 31.59329560678562 28.701444937442233 20.089756743276183 90-91 20.151215979359108 30.731410429576528 28.340700789062446 20.77667280200192 92-93 19.772027386406485 31.55395605063801 27.974899036241336 20.69911752671417 94-95 19.480292884080672 32.14524336067339 27.849509133875777 20.524954621370163 96-97 20.03764004406156 32.33049584054302 27.34815739984996 20.28370671554546 98-99 20.4292458412829 31.277603897128202 27.208802665360476 21.084347596228422 100-101 20.47922203864129 31.31796207616247 26.84991414154347 21.352901743652776 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 280.0 1 360.5 2 479.0 3 669.5 4 763.5 5 738.0 6 902.5 7 1235.5 8 1727.5 9 2242.5 10 2691.5 11 3103.5 12 3257.0 13 3177.0 14 2980.0 15 2736.0 16 2579.0 17 2536.5 18 2680.0 19 2970.0 20 3469.5 21 4335.0 22 5516.0 23 7247.0 24 9595.0 25 12466.5 26 16544.0 27 21873.5 28 27731.5 29 33815.5 30 40994.0 31 49679.5 32 61791.0 33 86225.5 34 126778.5 35 203302.5 36 306259.5 37 411270.0 38 532309.5 39 596076.5 40 590624.5 41 539159.5 42 483480.5 43 488032.0 44 478826.5 45 452001.0 46 454880.5 47 414346.5 48 327702.5 49 275757.0 50 237390.0 51 202811.5 52 180807.5 53 156979.5 54 124545.5 55 91529.0 56 69385.0 57 54186.0 58 43268.5 59 33858.0 60 25908.5 61 19761.5 62 14626.5 63 10933.0 64 8365.5 65 6220.0 66 4555.5 67 3316.0 68 2496.0 69 1777.5 70 1227.0 71 938.0 72 683.5 73 510.5 74 403.0 75 339.0 76 276.0 77 249.0 78 212.5 79 162.0 80 127.5 81 97.0 82 85.0 83 67.5 84 49.0 85 49.0 86 40.0 87 34.0 88 32.0 89 29.5 90 30.5 91 20.0 92 13.0 93 13.5 94 12.5 95 10.5 96 6.0 97 3.0 98 2.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18760488477588028 2 0.17778170440395205 3 0.15138413969538853 4 0.14416856720400853 5 0.14851458033825557 6 0.1290230091032901 7 0.10351846079217461 8 0.09114720696619469 9 0.09120674139269122 10-11 0.07152465999293683 12-13 0.09113530008089538 14-15 0.0952788961650542 16-17 0.08797402203392939 18-19 0.08425907382054561 20-21 0.08209797413872141 22-23 0.10248256177113489 24-25 0.4487347862749809 26-27 0.2832886150411109 28-29 0.12385542088339087 30-31 0.23151152431707464 32-33 0.2629218877366464 34-35 0.2463594102853104 36-37 0.24881222865696762 38-39 0.2667975789015708 40-41 0.2665237205396867 42-43 0.29270696131286267 44-45 0.2684109618596269 46-47 0.28696188915594706 48-49 0.2707328044929917 50-51 0.28616412784089357 52-53 0.28515204259045246 54-55 0.2618264542891101 56-57 0.27850800059343916 58-59 0.26738101628123684 60-61 0.24354938535467396 62-63 0.23588730466456995 64-65 0.23422629416531665 66-67 0.243531525026725 68-69 0.24803232766986302 70-71 0.23468470924933993 72-73 0.22571287117631217 74-75 0.23501810203772053 76-77 0.2359587459763658 78-79 0.23794719582135004 80-81 0.24510918732888318 82-83 0.2712209867902633 84-85 0.2650829874184706 86-87 0.26491033758163063 88-89 0.26295165494989464 90-91 0.25415246671370684 92-93 0.23077329742851763 94-95 0.22959451578388623 96-97 0.22459362395817728 98-99 0.2494313866925316 100-101 0.25758760312255685 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8398502.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.82124007242108 #Duplication Level Percentage of deduplicated Percentage of total 1 91.40879187404816 39.14237821568589 2 3.8563280767017973 3.30265500740931 3 1.1993247778122775 1.5406972270650778 4 0.738624076189 1.265151955590376 5 0.48689907052609505 1.0424810995018297 6 0.3355544327925424 0.8621314154384724 7 0.24869983833869652 0.7454744838431544 8 0.1772991347127229 0.6073735049732826 9 0.14385626468142904 0.5544093281260699 >10 1.0718588516881922 9.231368311119548 >50 0.14695058107124698 4.352339465723077 >100 0.1414463323747779 13.223185410484122 >500 0.025581204987751623 7.557301009534139 >1k 0.018109548095157753 14.1104717109648 >5k 4.508669895824191E-4 1.1751619059262268 >10k+ 2.2506899054393353E-4 1.287419948614805 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 17300 0.20598911567801018 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 16190 0.1927724729957795 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT 13775 0.1640173449979532 Illumina Single End PCR Primer 1 (96% over 32bp) TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 13420 0.15979040071669923 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 13329 0.1587068741544623 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 11774 0.14019166751404 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 10883 0.1295826327123575 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 10194 0.12137878874113502 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 9894 0.11780672315134294 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0021313324685759438 0.0 0.0 2 0.002250401321569013 0.0 1.1906885299306947E-5 3 0.002500445912854459 0.0 1.1906885299306947E-5 4 0.002679049192344063 0.0 1.1906885299306947E-5 5 0.0030005350954253507 0.0 1.1906885299306947E-5 6 0.0033339278838059455 0.0 1.1906885299306947E-5 7 0.0037268550986830746 0.0 1.1906885299306947E-5 8 0.004322199363648422 0.0 1.1906885299306947E-5 9 0.004727033463824858 0.0 2.3813770598613895E-5 10-11 0.0075311049518116445 0.0 3.5720655897920846E-5 12-13 0.01037089709569635 0.0 4.167409854757432E-5 14-15 0.013258316780778287 0.0 4.762754119722779E-5 16-17 0.015705181709785864 0.0 4.762754119722779E-5 18-19 0.018854552871452553 0.0 4.762754119722779E-5 20-21 0.02224801518175503 0.0 7.144131179584169E-5 22-23 0.02661784208660068 0.0 7.144131179584169E-5 24-25 0.0316961286667551 0.0 8.334819709514864E-5 26-27 0.037125668363239064 0.0 8.334819709514864E-5 28-29 0.043043390356994615 0.0 8.334819709514864E-5 30-31 0.06754776030296832 0.0 8.334819709514864E-5 32-33 0.07437635902212085 0.0 8.334819709514864E-5 34-35 0.08085965806759349 0.0 8.334819709514864E-5 36-37 0.08802760301777626 0.0 8.334819709514864E-5 38-39 0.09515982731206113 0.0 8.334819709514864E-5 40-41 0.1030838594787499 0.0 8.334819709514864E-5 42-43 0.11132342410587032 0.0 8.334819709514864E-5 44-45 0.12092037365711171 0.0 8.334819709514864E-5 46-47 0.1307495074716896 0.0 8.334819709514864E-5 48-49 0.1414359370278176 0.0 8.334819709514864E-5 50-51 0.15289631412840052 0.0 8.334819709514864E-5 52-53 0.16417808794949384 0.0 8.334819709514864E-5 54-55 0.1768648742359054 0.0 8.334819709514864E-5 56-57 0.19056374577275803 0.0 8.334819709514864E-5 58-59 0.20608437076040464 0.0 8.334819709514864E-5 60-61 0.22265280165439028 0.0 8.334819709514864E-5 62-63 0.24009638861787497 0.0 8.334819709514864E-5 64-65 0.259647494279337 0.0 9.525508239445558E-5 66-67 0.2797403632219174 0.0 9.525508239445558E-5 68-69 0.3028278138172736 0.0 9.525508239445558E-5 70-71 0.32687972212187366 0.0 9.525508239445558E-5 72-73 0.35306891633769927 0.0 1.0716196769376254E-4 74-75 0.38258608499468116 0.0 1.0716196769376254E-4 76-77 0.4159848982592372 0.0 1.0716196769376254E-4 78-79 0.4533189371152141 0.0 1.0716196769376254E-4 80-81 0.49521926648347525 0.0 1.1906885299306948E-4 82-83 0.5414775158712828 0.0 1.1906885299306948E-4 84-85 0.5938082767617368 0.0 1.1906885299306948E-4 86-87 0.6525985229270649 0.0 1.1906885299306948E-4 88-89 0.7179911369908587 0.0 1.1906885299306948E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 5565 0.0 85.19238 9 CGGGAGA 8050 0.0 83.15326 4 AGAGCGG 5965 0.0 81.86444 8 GAGAGCG 7045 0.0 77.46108 7 AAGCGGC 2220 0.0 77.09393 9 CGGAAAG 3680 0.0 75.79786 5 TCGGGAG 8970 0.0 71.71931 3 ATTCGGA 1145 0.0 71.690865 2 ACCGGAA 1295 0.0 71.43873 3 TCGGGAA 3030 0.0 69.67648 3 TCGGAGA 2005 0.0 67.90717 4 GTTCGGA 1050 0.0 67.78369 2 GATCCGG 1600 0.0 65.845116 1 GAACCGG 1095 0.0 65.44153 1 GGATCGG 3080 0.0 65.020805 1 CGGAGAG 3470 0.0 62.202713 5 GATCGGG 13250 0.0 61.925526 1 AACCGGA 1130 0.0 60.88535 2 ATCGGGA 13090 0.0 60.207863 2 AGAGCGT 17180 0.0 58.91736 8 >>END_MODULE