FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n02_34302-1.342000000358e7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n02_34302-1.342000000358e7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8398502
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC173000.20598911567801018No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG161900.1927724729957795No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT137750.1640173449979532Illumina Single End PCR Primer 1 (96% over 32bp)
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA134200.15979040071669923No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA133290.1587068741544623No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC117740.14019166751404No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG108830.1295826327123575No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC101940.12137878874113502No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA98940.11780672315134294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC55650.085.192389
CGGGAGA80500.083.153264
AGAGCGG59650.081.864448
GAGAGCG70450.077.461087
AAGCGGC22200.077.093939
CGGAAAG36800.075.797865
TCGGGAG89700.071.719313
ATTCGGA11450.071.6908652
ACCGGAA12950.071.438733
TCGGGAA30300.069.676483
TCGGAGA20050.067.907174
GTTCGGA10500.067.783692
GATCCGG16000.065.8451161
GAACCGG10950.065.441531
GGATCGG30800.065.0208051
CGGAGAG34700.062.2027135
GATCGGG132500.061.9255261
AACCGGA11300.060.885352
ATCGGGA130900.060.2078632
AGAGCGT171800.058.917368