FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n02_34298-1.342000000357ce.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n02_34298-1.342000000357ce.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5348509
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC147430.275646913934332No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG138040.2580906192735209No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA97670.18261163999163132No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA93680.17515161702074356No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA74520.1393285493209416No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72760.13603791262200363No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA70050.1309710799776162No Hit
GTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGT61750.11545273645421555No Hit
TTGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC60380.11289127493288316No Hit
TGTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAG58890.11010545181844138No Hit
GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT57430.1073757191022769No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT57410.10733832550342536Illumina Single End PCR Primer 1 (96% over 32bp)
TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT56010.10472077358381561No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGG15550.073.501888
CGGGAGA20200.072.818494
GAGCGGC15350.072.604439
GAGAGCG18000.067.451487
CGGAAAG10650.066.383455
AAGCGGC6700.062.9968539
TCGGGAA7300.060.4521453
GAACCGG3750.058.2259371
ATTCGGA3050.056.0158922
TCGGGAG25150.055.470363
CGTGCGC352.7985137E-454.247771
GTTCGGA3700.053.871142
AACCGGA3500.052.8816682
ACCGGAA3600.052.7240943
AAGCGTC3100.050.4842539
GATCCGG5300.049.2579961
AAAGCGG9350.049.200778
GGATCGG10350.049.0719531
TCGGAGA6400.045.966674
TCGGAAA10850.045.918844
Produced by FastQC (version 0.11.2)