##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n02_34274-2.3420000003583a.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4635548 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.271438242037405 33.0 33.0 33.0 33.0 33.0 2 32.25935790115861 33.0 33.0 33.0 33.0 33.0 3 32.244061974981165 33.0 33.0 33.0 33.0 33.0 4 36.05621773304904 37.0 37.0 37.0 37.0 37.0 5 36.058215770821484 37.0 37.0 37.0 37.0 37.0 6 36.04387852310018 37.0 37.0 37.0 33.0 37.0 7 35.982119913330635 37.0 37.0 37.0 33.0 37.0 8 35.95285778509898 37.0 37.0 37.0 33.0 37.0 9 35.92946767027329 37.0 37.0 37.0 33.0 37.0 10-11 35.85891484674519 37.0 37.0 37.0 33.0 37.0 12-13 35.86465472906332 37.0 37.0 37.0 33.0 37.0 14-15 37.73537885919852 40.0 37.0 40.0 33.0 40.0 16-17 37.70428426153715 40.0 37.0 40.0 33.0 40.0 18-19 37.6485848059388 40.0 37.0 40.0 33.0 40.0 20-21 37.56242206962369 40.0 37.0 40.0 33.0 40.0 22-23 37.60963946441716 40.0 37.0 40.0 33.0 40.0 24-25 37.42082349271327 40.0 37.0 40.0 33.0 40.0 26-27 37.382585510925566 40.0 37.0 40.0 33.0 40.0 28-29 37.38390984194318 40.0 37.0 40.0 33.0 40.0 30-31 37.33932234117735 40.0 37.0 40.0 33.0 40.0 32-33 37.27930958756117 40.0 37.0 40.0 33.0 40.0 34-35 37.22502086053257 40.0 37.0 40.0 33.0 40.0 36-37 37.14410852826893 40.0 37.0 40.0 33.0 40.0 38-39 37.11049470310738 40.0 37.0 40.0 33.0 40.0 40-41 37.00677740797852 40.0 37.0 40.0 33.0 40.0 42-43 36.92549661873849 40.0 37.0 40.0 33.0 40.0 44-45 36.77962724148256 40.0 37.0 40.0 33.0 40.0 46-47 36.70014602372794 40.0 37.0 40.0 33.0 40.0 48-49 36.5786665352187 40.0 37.0 40.0 33.0 40.0 50-51 35.38941329051063 37.0 35.0 38.5 30.0 38.5 52-53 35.78800262665817 37.0 37.0 38.5 33.0 40.0 54-55 36.57964657037313 38.5 37.0 40.0 33.0 40.0 56-57 36.60434990641883 38.5 37.0 40.0 33.0 40.0 58-59 36.45340033152499 37.0 37.0 40.0 33.0 40.0 60-61 36.200506283183785 37.0 37.0 40.0 33.0 40.0 62-63 35.95945053314085 37.0 37.0 40.0 33.0 40.0 64-65 35.7242436061497 37.0 37.0 40.0 33.0 40.0 66-67 35.490275583383024 37.0 37.0 40.0 30.0 40.0 68-69 35.27237966255554 37.0 37.0 37.0 27.0 40.0 70-71 34.91427885117358 37.0 33.0 37.0 27.0 40.0 72-73 34.6613179283226 37.0 33.0 37.0 27.0 40.0 74-75 34.41091301395218 37.0 33.0 37.0 27.0 38.5 76-77 34.17038837695134 37.0 33.0 37.0 27.0 37.0 78-79 33.93212442196694 37.0 33.0 37.0 27.0 37.0 80-81 33.71021570696712 37.0 33.0 37.0 27.0 37.0 82-83 33.497694231620514 37.0 33.0 37.0 27.0 37.0 84-85 33.31665900126587 37.0 33.0 37.0 27.0 37.0 86-87 33.16786645289834 37.0 33.0 37.0 27.0 37.0 88-89 33.01566945267312 37.0 33.0 37.0 27.0 37.0 90-91 32.85697354444394 37.0 33.0 37.0 27.0 37.0 92-93 32.72327122920527 37.0 33.0 37.0 27.0 37.0 94-95 32.60474565250969 35.0 33.0 37.0 27.0 37.0 96-97 32.47094259405792 33.0 33.0 37.0 22.0 37.0 98-99 32.32368772796657 33.0 33.0 37.0 22.0 37.0 100-101 31.05481175041225 33.0 30.0 37.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.28831619024946775 2306 2 0.31349558447493564 2306 3 0.25239271429116883 2306 4 0.33955853174048656 2306 5 0.3770221951822066 2306 6 0.30190917772773673 2306 7 0.3390640439005139 2306 8 0.30183049975325105 2306 9 0.37988848229746 2306 10-11 0.3512850326146051 2306 12-13 0.3832511236889431 2306 14-15 0.5173877333815042 2306 16-17 0.4544940085721052 2306 18-19 0.4626783139335231 2306 20-21 0.5221394440214411 2306 22-23 0.48547544560471323 2306 24-25 0.4787257494377357 2306 26-27 0.4881683688301379 2306 28-29 0.4287647571974844 2306 30-31 0.5185552241724665 2306 32-33 0.32006713321516855 2306 34-35 0.35857657328497083 2306 36-37 0.3518552784946323 2306 38-39 0.2082123260006128 2306 40-41 0.26309373611390896 2306 42-43 0.2501470939333643 2306 44-45 0.30435190247753496 2306 46-47 0.3732940386850956 2306 48-49 0.2877111594323267 2306 50-51 0.2911461886647686 2306 52-53 0.2640368196423495 2306 54-55 0.39435777670171035 2306 56-57 0.2864074366852094 2306 58-59 0.37174332038794233 2306 60-61 0.5024776837323301 2306 62-63 0.44407516196667274 2306 64-65 0.31339213261568943 2306 66-67 0.2886105777144081 2306 68-69 0.3201086176701864 2306 70-71 0.3646619900741257 2306 72-73 0.3845631425123912 2306 74-75 0.3148546082465984 2306 76-77 0.32116972962203505 2306 78-79 0.3220953901669361 2306 80-81 0.3613989115365044 2306 82-83 0.23238918923537 2306 84-85 0.2423565505143941 2306 86-87 0.3795412233395865 2306 88-89 0.37964346192293874 2306 90-91 0.2623521735430643 2306 92-93 0.2925474738361302 2306 94-95 0.2720219354608773 2306 96-97 0.1838906519379364 2306 98-99 0.10803145565696326 2306 100-101 -0.103634809455869 2307 1 0.221732942493297 2307 2 0.2225765008181142 2307 3 0.17339621065777777 2307 4 0.26816077812843275 2307 5 0.240801423170808 2307 6 0.30522796545321285 2307 7 0.30150696318091974 2307 8 0.29959816553955676 2307 9 0.2360605280282897 2307 10-11 0.27898929601358446 2307 12-13 0.2754255063538764 2307 14-15 0.4333760065498282 2307 16-17 0.36939612937995037 2307 18-19 0.45807155228373375 2307 20-21 0.5344066917093571 2307 22-23 0.382502178805737 2307 24-25 0.27495443168998435 2307 26-27 0.388217556876036 2307 28-29 0.371653922476348 2307 30-31 0.25706976526306136 2307 32-33 0.2281884309363491 2307 34-35 0.2562931437337923 2307 36-37 0.280251738159933 2307 38-39 0.18407419592935526 2307 40-41 0.08667192553205183 2307 42-43 0.07711489182470643 2307 44-45 0.12044663263091593 2307 46-47 0.13174007789299225 2307 48-49 0.05937583348121933 2307 50-51 0.14204377011866143 2307 52-53 0.060989469623230264 2307 54-55 0.13528865545116986 2307 56-57 0.1388146896131488 2307 58-59 0.29207504858484157 2307 60-61 0.24319449409836125 2307 62-63 0.22081870252221591 2307 64-65 0.21447848574369743 2307 66-67 0.11444870688222153 2307 68-69 0.22446261299430859 2307 70-71 0.029024297174167657 2307 72-73 0.20569748911946562 2307 74-75 0.12250932221811439 2307 76-77 0.18393764134855672 2307 78-79 0.21976964885791261 2307 80-81 0.19513985315631288 2307 82-83 0.09755095265692404 2307 84-85 0.05819028287320549 2307 86-87 0.1121227324489169 2307 88-89 0.2646837710246359 2307 90-91 0.23208134817768666 2307 92-93 0.10089021814763299 2307 94-95 -0.06528970694805025 2307 96-97 0.14909482750111636 2307 98-99 0.15294099482520807 2307 100-101 0.049004430357936 2201 1 0.23775619164989337 2201 2 0.26843360364613034 2201 3 0.2881345951259533 2201 4 0.3205778541599358 2201 5 0.30217839328940244 2201 6 0.323170014586843 2201 7 0.32938459951792964 2201 8 0.35800239766054887 2201 9 0.26253487137261544 2201 10-11 0.3040648459208555 2201 12-13 0.30122889490764493 2201 14-15 0.45104775079925474 2201 16-17 0.45528748377271455 2201 18-19 0.4912339877577452 2201 20-21 0.602801026702501 2201 22-23 0.528927476046384 2201 24-25 0.4064388360406994 2201 26-27 0.4121379524428619 2201 28-29 0.35328882038569276 2201 30-31 0.4416658823075821 2201 32-33 0.3799795193153912 2201 34-35 0.3697905981220373 2201 36-37 0.4142485996488361 2201 38-39 0.35172474963992784 2201 40-41 0.35933462425374074 2201 42-43 0.2975208407866745 2201 44-45 0.35417249772495296 2201 46-47 0.3197923273013643 2201 48-49 0.334811726749102 2201 50-51 0.3534610278374828 2201 52-53 0.3303611933546833 2201 54-55 0.44810174163669814 2201 56-57 0.3777368310075673 2201 58-59 0.4522285374266062 2201 60-61 0.418235591618604 2201 62-63 0.4205364998656407 2201 64-65 0.42096593558312634 2201 66-67 0.4108340101778438 2201 68-69 0.37509954167151704 2201 70-71 0.3671048511556734 2201 72-73 0.4653517356864043 2201 74-75 0.39901909364258614 2201 76-77 0.3286611119211855 2201 78-79 0.3347705705563584 2201 80-81 0.36520008468166054 2201 82-83 0.3614042792683989 2201 84-85 0.3186030597924301 2201 86-87 0.4316137749230151 2201 88-89 0.4522109459724746 2201 90-91 0.41161532304345627 2201 92-93 0.29531369624161385 2201 94-95 0.33347133330374135 2201 96-97 0.42671399988979175 2201 98-99 0.35963082994064877 2201 100-101 0.11226761419803566 1101 1 0.22140607339876794 1101 2 0.2038971919467656 1101 3 0.15507491793600536 1101 4 0.1883443497717323 1101 5 0.2156592753608848 1101 6 0.23625963640872527 1101 7 0.28546622983225944 1101 8 0.3013962955263665 1101 9 0.2872129165699491 1101 10-11 0.25432266574348006 1101 12-13 0.2837666177017937 1101 14-15 0.39565412315390347 1101 16-17 0.3563061420382567 1101 18-19 0.333533814826815 1101 20-21 0.44145084318483185 1101 22-23 0.25656448621457173 1101 24-25 0.28262658128824114 1101 26-27 0.28841075610905165 1101 28-29 0.176944494752739 1101 30-31 0.2223537476625168 1101 32-33 0.1454725267250936 1101 34-35 0.08165353090918614 1101 36-37 0.06719380778725537 1101 38-39 0.1058619283992428 1101 40-41 -0.06362635126587435 1101 42-43 0.045313136180709535 1101 44-45 0.14084594137626993 1101 46-47 0.07409597814261559 1101 48-49 0.18612299953666422 1101 50-51 0.16245147806987603 1101 52-53 0.219843649960481 1101 54-55 0.29603589429974164 1101 56-57 0.19017110886370858 1101 58-59 0.18971541418827087 1101 60-61 0.2736037314049753 1101 62-63 0.3005869229369793 1101 64-65 0.356320774555293 1101 66-67 0.22496458260616237 1101 68-69 0.30976433521647095 1101 70-71 0.36978760300360136 1101 72-73 0.2828271340115478 1101 74-75 0.3254441972256856 1101 76-77 0.284175601349439 1101 78-79 0.39627196056434144 1101 80-81 0.24900019816069374 1101 82-83 0.18281050386635656 1101 84-85 0.32401272436135997 1101 86-87 0.315618079463853 1101 88-89 0.316972764600294 1101 90-91 0.32972631584599554 1101 92-93 0.2851151621791743 1101 94-95 0.278911761303128 1101 96-97 0.35117254605282966 1101 98-99 0.240274712422206 1101 100-101 0.1852601973751682 2304 1 0.1711879648486132 2304 2 0.12484494181314432 2304 3 0.1469704687668525 2304 4 0.20834874128763659 2304 5 0.17782865793973457 2304 6 0.24690957346317788 2304 7 0.2217278584802429 2304 8 0.15961340138598956 2304 9 0.18086990618287757 2304 10-11 0.2136946468500298 2304 12-13 0.1961684308956464 2304 14-15 0.3212857065041632 2304 16-17 0.25872050492316845 2304 18-19 0.3386511426127399 2304 20-21 0.39409670961938303 2304 22-23 0.3189538679729296 2304 24-25 0.36713187366348876 2304 26-27 0.3319050494012785 2304 28-29 0.1809537604425131 2304 30-31 0.2783410496409928 2304 32-33 0.1750705482654169 2304 34-35 0.11916383629911564 2304 36-37 0.2627276594693484 2304 38-39 0.1897357943683744 2304 40-41 0.2126773763690366 2304 42-43 0.11263074629088266 2304 44-45 0.19389024882268302 2304 46-47 0.2513614594406448 2304 48-49 0.16267350175059647 2304 50-51 0.1338246852386149 2304 52-53 0.21442323749960934 2304 54-55 0.4031096438820114 2304 56-57 0.34557449106092974 2304 58-59 0.320338375830687 2304 60-61 0.2860904258271475 2304 62-63 0.2371547751426064 2304 64-65 0.2384706201441631 2304 66-67 0.1614437796244843 2304 68-69 0.27087569532938716 2304 70-71 0.3969114549937487 2304 72-73 0.29200179619112276 2304 74-75 0.28407459904209276 2304 76-77 0.30340585679171994 2304 78-79 0.33971918521534405 2304 80-81 0.4424497587244147 2304 82-83 0.3273721851745748 2304 84-85 0.2906233339893234 2304 86-87 0.42326044129936236 2304 88-89 0.419757048462742 2304 90-91 0.1964225658739167 2304 92-93 0.26310911245722224 2304 94-95 0.27172222344739794 2304 96-97 0.30392491550778544 2304 98-99 0.25540781345875274 2304 100-101 0.2313216952412418 2202 1 0.14100744235583562 2202 2 0.11862602429830815 2202 3 0.12363838412080241 2202 4 0.1597868489058527 2202 5 0.23963490954188416 2202 6 0.17089486342882765 2202 7 0.26355866579393705 2202 8 0.18565740088044436 2202 9 0.08829935724673987 2202 10-11 0.21151822715594193 2202 12-13 0.15831393989382292 2202 14-15 0.24522895480438223 2202 16-17 0.2616517577030706 2202 18-19 0.3089350278214553 2202 20-21 0.4115829234295205 2202 22-23 0.34197544860310103 2202 24-25 0.17177708781768075 2202 26-27 0.1885181806313838 2202 28-29 0.1749832916259635 2202 30-31 0.1435159952776104 2202 32-33 0.07242416513199856 2202 34-35 -0.035071244189232686 2202 36-37 0.02611352755663887 2202 38-39 0.020413961520702628 2202 40-41 -0.02300134473259874 2202 42-43 -0.04368562406435217 2202 44-45 0.05895092820169623 2202 46-47 0.03534865330242809 2202 48-49 0.052599649424713846 2202 50-51 0.010004442923971624 2202 52-53 0.039564185919296335 2202 54-55 0.1459572162032572 2202 56-57 0.05755795770490124 2202 58-59 -0.027933253615245235 2202 60-61 0.21347296826925088 2202 62-63 0.15755550953967656 2202 64-65 0.0772467788160327 2202 66-67 0.10329287863650904 2202 68-69 0.1731496815777973 2202 70-71 0.20952265503638046 2202 72-73 0.2296077085358732 2202 74-75 0.1582004881632102 2202 76-77 0.15826737109092193 2202 78-79 0.2491762206801198 2202 80-81 0.16583912270289147 2202 82-83 0.1316887660630286 2202 84-85 0.11303472565617056 2202 86-87 0.14380205101429766 2202 88-89 0.13300215494609802 2202 90-91 0.1870952617615984 2202 92-93 0.14602190459238074 2202 94-95 0.1259541199456251 2202 96-97 0.21306335702259815 2202 98-99 0.21674017036844617 2202 100-101 -0.07471836650217512 1102 1 0.05745300434950451 1102 2 0.07174728155280974 1102 3 0.09777980084494686 1102 4 0.09348572311125025 1102 5 0.0762096650812154 1102 6 0.08374971420133903 1102 7 0.15031295689433222 1102 8 0.18615611400069554 1102 9 0.14661212394232592 1102 10-11 0.12536661069666621 1102 12-13 0.09573017939234774 1102 14-15 0.17100644344503024 1102 16-17 0.139562869149934 1102 18-19 0.17962261502506038 1102 20-21 0.29950537243520614 1102 22-23 0.14451169712052803 1102 24-25 0.04810082480724276 1102 26-27 -0.001830163102944482 1102 28-29 -0.06552026799558774 1102 30-31 0.055837120735169776 1102 32-33 -0.0807030776097335 1102 34-35 -0.09905494041935725 1102 36-37 -0.027761436196293232 1102 38-39 -0.028548631508584776 1102 40-41 -0.04345526548823386 1102 42-43 -0.07117934947227411 1102 44-45 0.01193832555495078 1102 46-47 -0.06680452403244175 1102 48-49 -0.10359050659892688 1102 50-51 -0.11632612216995142 1102 52-53 -0.03073768602730098 1102 54-55 0.04787364717926579 1102 56-57 0.10224836564199791 1102 58-59 0.12766378711337723 1102 60-61 0.1958525074332229 1102 62-63 0.27608006950467257 1102 64-65 0.219231368801438 1102 66-67 0.22741814035629915 1102 68-69 0.11100611588940268 1102 70-71 0.1989107929629199 1102 72-73 0.18386214055671957 1102 74-75 0.17349837656466605 1102 76-77 0.11681938294102423 1102 78-79 0.07761667002169048 1102 80-81 0.08698750525572052 1102 82-83 0.08160392323437549 1102 84-85 0.06146774834465418 1102 86-87 0.11004154953506884 1102 88-89 0.20177880271295123 1102 90-91 0.16642077870721295 1102 92-93 0.2012521744216329 1102 94-95 0.2168979324917899 1102 96-97 0.2144372297316295 1102 98-99 0.15845015433547616 1102 100-101 0.10936683313125783 2305 1 0.08292064132680821 2305 2 0.10260749845250672 2305 3 0.09973332352723219 2305 4 0.11655515504878622 2305 5 0.11621812382610841 2305 6 0.13355273365072406 2305 7 0.14754388843311972 2305 8 0.11064855029089671 2305 9 0.04007662504544385 2305 10-11 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5650.0 9 10638.0 10 9949.0 11 11153.0 12 8300.0 13 7469.0 14 7351.0 15 7824.0 16 8355.0 17 9154.0 18 9935.0 19 11071.0 20 12604.0 21 14457.0 22 16502.0 23 18222.0 24 20532.0 25 24145.0 26 28814.0 27 35452.0 28 43362.0 29 51957.0 30 63597.0 31 81742.0 32 111315.0 33 160322.0 34 242705.0 35 407027.0 36 825255.0 37 1689077.0 38 677406.0 39 748.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 33.03619519086207 32.81277422400281 16.123485202818774 18.02754538231635 2 30.693884131557226 25.596336560461815 29.967104857938846 13.742674450042111 3 31.954856677404784 23.80965653134905 25.250034029258682 18.98545276198748 4 28.036411181743077 23.348473692761317 29.574665650395964 19.04044947509964 5 29.228346470302412 21.71671375493919 30.635485350567276 18.419454424191123 6 27.367680470872664 22.03943005537264 32.01966059253954 18.573228881215165 7 31.538950766921676 22.743166271643066 25.329201332462304 20.388681628972957 8 27.5283110332326 27.062378742190106 25.153131676939505 20.256178547637788 9 25.98879840453488 24.748624848470975 28.511597395536203 20.75097935145794 10-11 22.7753481571674 29.03547929731162 30.9228978204657 17.26627472505528 12-13 22.835510150936624 28.48492890209319 29.99606577115096 18.68349517581922 14-15 22.255080744510053 28.48009000283958 30.706451031264542 18.558378221385823 16-17 22.353620569799652 28.36963833761813 30.965975063775364 18.31076602880685 18-19 21.9268254397683 28.375054761195845 31.07945355613646 18.618666242899398 20-21 21.969434361777914 29.02586993383566 30.456971048823906 18.54772465556252 22-23 22.180786829282198 28.993274357993158 30.226074776769323 18.59986403595532 24-25 21.565087214458643 29.39978764572268 30.284886081432955 18.750239058385723 26-27 21.399069273174845 29.374306284434283 30.473953289286733 18.752671153104142 28-29 21.14079691254897 29.656332718770678 30.374335293302558 18.828535075377797 30-31 21.174561281634457 29.64371111326154 30.48035883515888 18.701368769945123 32-33 20.624625782180136 29.66927580848354 31.016935843923914 18.68916256541241 34-35 20.58240412342327 30.25753444566736 30.64689533192999 18.513166098979376 36-37 20.690676168036518 29.91509455590367 30.843956697077225 18.55027257898259 38-39 20.853429910109313 29.255633215630027 30.989329692523864 18.901607181736797 40-41 20.98042335361731 29.658375368839323 30.117936647840686 19.24326462970268 42-43 21.38393324192769 29.262006349204977 30.358289858311878 18.99577055055546 44-45 20.793049250985124 29.50873186384789 30.525359056305177 19.172859828861807 46-47 21.0630928767433 29.693003641981285 30.156578690433754 19.08732479084166 48-49 20.77937768939937 29.875964484514704 30.139736816088618 19.204921009997303 50-51 20.90320345818308 29.36500089033631 30.03717776154089 19.69461788993972 52-53 21.275689553295432 29.27442339884579 29.8094775079086 19.64040953995018 54-55 21.432200816097684 29.411216930392893 29.723115586150566 19.43346666735886 56-57 21.387850365182217 29.222398448535365 29.923123404308715 19.4666277819737 58-59 21.358104196753597 29.50250458821178 29.870729674935685 19.26866154009894 60-61 20.811537885097376 29.708589982012874 29.57962854868321 19.90024358420654 62-63 20.974809887473278 29.722548702650275 29.447865430714486 19.85477597916196 64-65 20.686585206305338 29.692701761442457 29.802587831547672 19.818125200704532 66-67 20.896356798726952 29.215800260950076 29.831842267294107 20.056000673028866 68-69 20.714193100690352 29.2602698010918 30.55227502941119 19.473262068806665 70-71 20.722661795594455 30.268708370789394 29.78667938785206 19.221950445764087 72-73 20.665100803563465 30.44711428007687 29.475367387702477 19.412417528657187 74-75 20.79899910753539 30.068391290912107 29.479877161231617 19.65273244032089 76-77 20.910962272303223 29.867770761132313 29.520384097545065 19.7008828690194 78-79 20.655175650186607 29.71532615620568 29.92519247928369 19.704305714324022 80-81 20.58464451171523 29.97382869854818 29.739409446886057 19.702117342850535 82-83 20.289812903485405 30.031493548776496 29.6603443046606 20.0183492430775 84-85 20.349265441519236 30.06923225849017 29.73982471832822 19.841677581662374 86-87 20.454546191988605 29.85445674505081 30.119660966903012 19.571336096057575 88-89 20.61483221917846 30.293605622054905 29.708825733256962 19.382736425509677 90-91 20.930992100420745 29.933819597164174 29.344906477395437 19.790281825019644 92-93 20.55625935848564 30.21856838158759 29.36343430649871 19.861737953428054 94-95 20.15897160720864 30.571225894746423 29.32185744452444 19.9479450535205 96-97 20.36048318130759 30.70304112749513 29.17493105055068 19.761544640646605 98-99 20.591337034641914 30.342093481472187 29.021052904777456 20.04551657910844 100-101 20.592267694321276 30.162126408395373 28.98904625538059 20.256559641902758 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 199.0 1 221.0 2 246.0 3 252.0 4 253.5 5 312.0 6 434.5 7 602.0 8 870.5 9 1163.5 10 1438.0 11 1633.5 12 1651.5 13 1541.0 14 1339.5 15 1126.5 16 987.0 17 972.0 18 1043.5 19 1234.0 20 1602.0 21 2203.0 22 3122.5 23 4513.5 24 6657.5 25 9678.5 26 14295.0 27 20201.0 28 26794.5 29 34253.5 30 42362.0 31 51168.5 32 65022.5 33 88139.5 34 117165.0 35 154294.5 36 201175.5 37 257279.0 38 310106.5 39 326794.0 40 332170.5 41 318426.0 42 283843.5 43 270117.5 44 252510.0 45 237092.5 46 239713.5 47 221766.5 48 175471.0 49 131268.0 50 99536.5 51 80114.0 52 63842.0 53 47682.5 54 36881.5 55 28576.5 56 19816.5 57 12880.5 58 8475.0 59 5905.0 60 4300.5 61 3190.5 62 2257.0 63 1442.0 64 965.5 65 700.0 66 501.5 67 367.0 68 296.5 69 227.5 70 166.5 71 132.5 72 107.0 73 90.0 74 76.0 75 64.5 76 52.5 77 36.5 78 34.5 79 36.5 80 27.5 81 24.0 82 19.0 83 16.0 84 13.5 85 10.0 86 9.0 87 8.5 88 6.0 89 4.5 90 6.0 91 3.5 92 4.0 93 4.5 94 1.5 95 3.0 96 2.5 97 1.5 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19104537370770402 2 0.18166136991786083 3 0.15484684874366525 4 0.14798681838695232 5 0.15115796449524413 6 0.1334470056183217 7 0.10835827824455706 8 0.09498337629121735 9 0.0953932523188197 10-11 0.07508281652999818 12-13 0.09489708660119581 14-15 0.09886641234218695 16-17 0.09175829912666204 18-19 0.08790762170945053 20-21 0.08573959324765917 22-23 0.10596373934645914 24-25 0.45495160442735144 26-27 0.285759094717604 28-29 0.12875500372340012 30-31 0.2403275729212598 32-33 0.27120849573772077 34-35 0.2549213167461538 36-37 0.2574452901792841 38-39 0.27540433191501845 40-41 0.2754690491825346 42-43 0.3025424394267949 44-45 0.2784891883332888 46-47 0.29663159566031894 48-49 0.28046306499253165 50-51 0.2955206159012915 52-53 0.29508916745118374 54-55 0.27120849573772077 56-57 0.2876682541093307 58-59 0.27681732558912125 60-61 0.2520737569754428 62-63 0.244048815803439 64-65 0.24189157355290034 66-67 0.2518580327503889 68-69 0.2563343104202567 70-71 0.24249560138305115 72-73 0.23374798405711686 74-75 0.2432290637482343 76-77 0.2438978088459013 78-79 0.24622763047648302 80-81 0.2541447095359599 82-83 0.28074350648510166 84-85 0.27403448308592643 86-87 0.27387268991713604 88-89 0.2720713926379362 90-91 0.2631943407769696 92-93 0.23877435850087197 94-95 0.23754473041806493 96-97 0.23261543187558406 98-99 0.25796302831941337 100-101 0.26608504539269145 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4635548.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.26592101423787 #Duplication Level Percentage of deduplicated Percentage of total 1 90.35196219487183 45.41624595168833 2 4.536474421042831 4.560601298624988 3 1.2867679546224249 1.9404172911210968 4 0.8655024355113696 1.7402110824418004 5 0.5544588641768468 1.393519273617871 6 0.2871957780214706 0.8661696176189901 7 0.2240058530879655 0.7881902362632652 8 0.17096998267555294 0.6875170915979968 9 0.17955211437705004 0.812281715929458 >10 1.2459725244889484 12.520895182237199 >50 0.16744265061492855 5.82271190735155 >100 0.10291787917222918 10.095814756986849 >500 0.017115309905620612 6.135066292450047 >1k 0.009662037431001556 7.22035830207073 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 5.608829851400525E-4 0.0 0.0 2 6.47172675161599E-4 0.0 0.0 3 0.0011433383927854917 0.0 0.0 4 0.0018768007579686372 0.0 0.0 5 0.0022866767855709833 0.0 0.0 6 0.004400774191098873 0.0 0.0 7 0.005975561033992097 0.0 0.0 8 0.007766072101939189 0.0 0.0 9 0.009038845029757 0.0 0.0 10-11 0.01265222579940926 0.0 0.0 12-13 0.018595428199643278 0.0 0.0 14-15 0.026490934836614785 0.0 0.0 16-17 0.03671626310416805 0.0 0.0 18-19 0.04888310939720611 0.0 1.0786211252693317E-5 20-21 0.06295911508197088 0.0 2.1572422505386633E-5 22-23 0.0798934667486994 0.0 2.1572422505386633E-5 24-25 0.09849968115959537 0.0 2.1572422505386633E-5 26-27 0.11756970265435715 0.0 2.1572422505386633E-5 28-29 0.1383331593157918 0.0 4.314484501077327E-5 30-31 0.16165294804411473 0.0 6.47172675161599E-5 32-33 0.18456286074483536 0.0 6.47172675161599E-5 34-35 0.20863768426084683 0.0 6.47172675161599E-5 36-37 0.23758787526307568 0.0 6.47172675161599E-5 38-39 0.2707878334988657 0.0 6.47172675161599E-5 40-41 0.30614503398519444 0.0 8.628969002154653E-5 42-43 0.3457735741275897 0.0 8.628969002154653E-5 44-45 0.38795844633687326 0.0 9.707590127423985E-5 46-47 0.4332928922319432 0.0 1.0786211252693317E-4 48-49 0.48341641592320905 0.0 1.0786211252693317E-4 50-51 0.5359129060900675 0.0 1.0786211252693317E-4 52-53 0.5921845701953685 0.0 1.0786211252693317E-4 54-55 0.6515842355639506 0.0 1.0786211252693317E-4 56-57 0.7120193772127912 0.0 1.0786211252693317E-4 58-59 0.7762189065888219 0.0 1.1864832377962649E-4 60-61 0.8450565068035105 0.0 1.294345350323198E-4 62-63 0.9188449779831855 0.0 1.294345350323198E-4 64-65 0.9949632707934424 0.0 1.294345350323198E-4 66-67 1.0748675237533942 0.0 1.294345350323198E-4 68-69 1.1559259013173846 0.0 1.294345350323198E-4 70-71 1.238494348456752 0.0 1.294345350323198E-4 72-73 1.3255498594772397 0.0 1.294345350323198E-4 74-75 1.4174268069276814 0.0 1.294345350323198E-4 76-77 1.5158401983972554 0.0 1.294345350323198E-4 78-79 1.6199055645632403 0.0 1.402207462850131E-4 80-81 1.7271744354712755 0.0 1.5100695753770643E-4 82-83 1.8422956681712712 0.0 1.5100695753770643E-4 84-85 1.9613430817672475 0.0 1.5100695753770643E-4 86-87 2.0881673536764156 0.0 1.8336559129578637E-4 88-89 2.2213447040134198 0.0 2.1572422505386634E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 475 0.0 61.88619 9 ATCGGGA 740 0.0 55.774 2 GCGATCG 35 2.8051066E-4 54.22178 1 CGGGAGA 800 0.0 48.619125 4 CGATCGC 40 5.4212887E-4 47.444054 1 AGAGCGG 680 0.0 46.01881 8 ACGACGG 55 5.0003055E-5 43.130955 1 CGACCGC 45 9.684102E-4 42.172493 1 GAGAGCG 865 0.0 40.013985 7 GATCCGG 235 0.0 36.340126 1 CGGAAAG 405 0.0 36.306965 5 ATTCGGA 185 0.0 35.900505 2 GGTCCGG 135 3.1286618E-10 35.143745 1 CGCGGAG 110 7.093695E-8 34.504765 1 CGAGGCG 140 4.620233E-10 33.88861 1 AAGCGGC 255 0.0 33.46786 9 CGACGGG 115 1.04833816E-7 33.00456 1 AGCGCCC 145 6.730261E-10 32.720036 1 GGACGGG 480 0.0 32.617783 1 CGAGGGG 600 0.0 32.4201 1 >>END_MODULE