FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n02_34274-2.3420000003583a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n02_34274-2.3420000003583a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4635548
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC4750.061.886199
ATCGGGA7400.055.7742
GCGATCG352.8051066E-454.221781
CGGGAGA8000.048.6191254
CGATCGC405.4212887E-447.4440541
AGAGCGG6800.046.018818
ACGACGG555.0003055E-543.1309551
CGACCGC459.684102E-442.1724931
GAGAGCG8650.040.0139857
GATCCGG2350.036.3401261
CGGAAAG4050.036.3069655
ATTCGGA1850.035.9005052
GGTCCGG1353.1286618E-1035.1437451
CGCGGAG1107.093695E-834.5047651
CGAGGCG1404.620233E-1033.888611
AAGCGGC2550.033.467869
CGACGGG1151.04833816E-733.004561
AGCGCCC1456.730261E-1032.7200361
GGACGGG4800.032.6177831
CGAGGGG6000.032.42011