FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n02_34271-1.342000000358da.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n02_34271-1.342000000358da.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5243229
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT125720.23977590908197985Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC47000.077.669899
ATCGGGA81800.071.219572
TCGGGAG73800.070.447053
CGGGAGA72100.069.9342654
AGAGCGG54050.069.0321358
ATTCGGA11350.066.877242
GATCGGG90800.066.6816251
CGGAAAG27200.065.5696265
TCGGAGA16350.064.696254
GTTCGGA12500.063.760772
ATCCGGA11800.061.5125432
GAGAGCG70600.060.3082967
TCGGGAA20800.059.7511943
AAGCGGC14750.059.4629
AATCGGA11200.059.3013082
GGATCGG29650.059.0531461
TCGGAAA21400.057.1870884
ATCGGAG14200.056.4555973
CGGAGAG31800.056.233875
GAGGGGC72200.055.2228249
Produced by FastQC (version 0.11.2)