##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n02_34271-1.342000000358da.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5243229 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.16295340142496 33.0 33.0 33.0 27.0 33.0 2 30.72323638734833 33.0 33.0 33.0 27.0 33.0 3 30.590975522907733 33.0 33.0 33.0 27.0 33.0 4 34.03404428835742 37.0 37.0 37.0 27.0 37.0 5 34.08988316169292 37.0 37.0 37.0 27.0 37.0 6 34.55767428811521 37.0 37.0 37.0 27.0 37.0 7 34.07683700254175 37.0 37.0 37.0 27.0 37.0 8 34.258266995395395 37.0 37.0 37.0 27.0 37.0 9 33.839845064939944 37.0 37.0 37.0 27.0 37.0 10-11 33.83446212248216 37.0 37.0 37.0 24.5 37.0 12-13 33.827864565900136 37.0 37.0 37.0 24.5 37.0 14-15 35.41593386441828 40.0 37.0 40.0 22.0 40.0 16-17 35.3213311873275 40.0 37.0 40.0 24.5 40.0 18-19 35.31922399345899 40.0 37.0 40.0 18.5 40.0 20-21 35.226999717159025 40.0 37.0 40.0 22.0 40.0 22-23 35.91083118818575 40.0 37.0 40.0 27.0 40.0 24-25 35.30570512941548 40.0 37.0 40.0 22.0 40.0 26-27 35.18169328480599 40.0 37.0 40.0 21.0 40.0 28-29 34.97061295625272 40.0 37.0 40.0 18.5 40.0 30-31 34.91565436108169 40.0 37.0 40.0 18.5 40.0 32-33 34.782100304983814 38.5 37.0 40.0 15.0 40.0 34-35 34.776468374736254 37.0 37.0 40.0 15.0 40.0 36-37 34.621281275336244 37.0 37.0 40.0 15.0 40.0 38-39 34.56725655125878 37.0 37.0 40.0 15.0 40.0 40-41 34.55309924475929 37.0 37.0 40.0 15.0 40.0 42-43 34.275473567910154 37.0 37.0 40.0 15.0 40.0 44-45 34.067182264974505 37.0 33.0 40.0 15.0 40.0 46-47 34.00473391492151 37.0 33.0 40.0 15.0 40.0 48-49 33.89087859790217 37.0 33.0 40.0 15.0 40.0 50-51 32.85616811701339 37.0 33.0 38.5 15.0 38.5 52-53 33.26574082497636 37.0 33.0 38.5 15.0 40.0 54-55 33.95319153521618 37.0 33.0 40.0 15.0 40.0 56-57 34.124559598674786 37.0 33.0 40.0 18.5 40.0 58-59 33.716905460356585 37.0 33.0 40.0 15.0 40.0 60-61 33.44809896725853 37.0 33.0 40.0 15.0 40.0 62-63 33.18158276130987 37.0 33.0 40.0 15.0 40.0 64-65 32.95878446278047 37.0 33.0 38.5 15.0 40.0 66-67 32.816122564930886 37.0 33.0 37.0 15.0 40.0 68-69 32.78465922430624 37.0 33.0 37.0 15.0 40.0 70-71 32.39766725809611 37.0 33.0 37.0 15.0 40.0 72-73 32.42875726007771 37.0 33.0 37.0 15.0 38.5 74-75 32.444248286695085 37.0 33.0 37.0 22.0 37.0 76-77 32.210085331005 37.0 33.0 37.0 22.0 37.0 78-79 31.881254948048234 37.0 33.0 37.0 15.0 37.0 80-81 31.528621294244445 37.0 33.0 37.0 15.0 37.0 82-83 31.225671814067248 35.0 33.0 37.0 15.0 37.0 84-85 31.01562186202434 33.0 33.0 37.0 15.0 37.0 86-87 30.84831312918051 33.0 33.0 37.0 15.0 37.0 88-89 30.692894588430143 33.0 33.0 37.0 15.0 37.0 90-91 30.53807091012046 33.0 27.0 37.0 15.0 37.0 92-93 30.401557227426075 33.0 27.0 37.0 15.0 37.0 94-95 30.281633035673245 33.0 27.0 37.0 15.0 37.0 96-97 30.13872815778216 33.0 27.0 37.0 15.0 37.0 98-99 29.98750636296832 33.0 27.0 37.0 15.0 37.0 100-101 28.7618967243277 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -0.12992369826876882 2306 2 -0.022172437961000213 2306 3 -0.09749730141988877 2306 4 -0.040709240001376656 2306 5 -0.0715723778755546 2306 6 -0.33085234583124645 2306 7 0.18712675988386707 2306 8 -0.36569119602232547 2306 9 -0.09568020159610313 2306 10-11 -0.12423050169260108 2306 12-13 -0.09444675362398414 2306 14-15 0.17546304463382967 2306 16-17 0.10084189086463624 2306 18-19 0.04881181070860663 2306 20-21 0.2250669091934654 2306 22-23 -0.006424197931359288 2306 24-25 0.28972379537875526 2306 26-27 -0.019949896328562033 2306 28-29 0.0623981300809362 2306 30-31 0.06881154095686526 2306 32-33 -0.03955026511976456 2306 34-35 -0.05149371853518403 2306 36-37 0.015515015148658051 2306 38-39 -0.07470902763822806 2306 40-41 -0.17421013720722556 2306 42-43 -0.08714955286632886 2306 44-45 -0.01853434742491089 2306 46-47 0.044393573079439363 2306 48-49 -0.049780486385230915 2306 50-51 -0.03395011712699869 2306 52-53 -0.10684860677311292 2306 54-55 0.07421691141498599 2306 56-57 -0.014361041906589378 2306 58-59 0.12448792964262623 2306 60-61 0.16128200926788594 2306 62-63 0.23967014951934829 2306 64-65 0.18517084351362456 2306 66-67 0.036090961044607184 2306 68-69 -0.08779050772940167 2306 70-71 0.016228090224799985 2306 72-73 -0.1320286746475574 2306 74-75 -0.256749163797636 2306 76-77 -0.1387915964032871 2306 78-79 -0.011229898949981276 2306 80-81 -0.11254324587938314 2306 82-83 -0.16211052597127917 2306 84-85 -0.03829132247916078 2306 86-87 0.10985831272893876 2306 88-89 0.058899681596550124 2306 90-91 0.05289038429017623 2306 92-93 -0.028520303773380107 2306 94-95 0.08484871722767906 2306 96-97 0.1606162342299129 2306 98-99 0.14235828417919194 2306 100-101 -0.09411781648496031 2307 1 -0.003968744974446992 2307 2 -0.0754286760190297 2307 3 -0.08347427980351085 2307 4 0.07055154088679672 2307 5 0.04600513096097103 2307 6 -0.19666708881009498 2307 7 0.20601823669172603 2307 8 -0.16776825597389688 2307 9 0.025016242971396707 2307 10-11 0.07953932343753678 2307 12-13 0.07055783224139844 2307 14-15 0.23329538430080987 2307 16-17 0.22908163000638382 2307 18-19 0.32775164370698207 2307 20-21 0.36969810088911004 2307 22-23 0.15238362383606585 2307 24-25 0.2828785797162183 2307 26-27 -0.06095943597335207 2307 28-29 0.07342267733400831 2307 30-31 0.06847739592411273 2307 32-33 0.04997541684580398 2307 34-35 -0.0037073718517177667 2307 36-37 -0.04804984281886249 2307 38-39 -0.06850672866070084 2307 40-41 -0.14015532921282414 2307 42-43 0.010629132051256818 2307 44-45 0.16032080813505445 2307 46-47 0.030086406915394548 2307 48-49 0.043457731597484894 2307 50-51 -0.07227542675065024 2307 52-53 0.019722272894625803 2307 54-55 0.08136144590176286 2307 56-57 0.1049067196463227 2307 58-59 0.15292620304875726 2307 60-61 0.07855365692815752 2307 62-63 0.1817577095727998 2307 64-65 0.2281450788358157 2307 66-67 0.014970358133290063 2307 68-69 -0.006922932148441419 2307 70-71 -0.011618572964216867 2307 72-73 -0.1392069159290017 2307 74-75 -0.19517785750883831 2307 76-77 -0.08099053813431567 2307 78-79 -0.002324713053898364 2307 80-81 -0.008276500505484563 2307 82-83 -0.10067821356825135 2307 84-85 0.02822288002180784 2307 86-87 0.08611243559231951 2307 88-89 0.25020054247555024 2307 90-91 0.22247430562672932 2307 92-93 0.04662799990419231 2307 94-95 0.026336768977433422 2307 96-97 0.05726964703665516 2307 98-99 0.17831342155490404 2307 100-101 0.03806561763293459 2201 1 -0.021797135708730764 2201 2 -0.03599298607915813 2201 3 -0.04534526093528868 2201 4 0.04084439373790616 2201 5 -0.03556642009601063 2201 6 -0.062029928282875346 2201 7 0.3044253020942591 2201 8 -0.051120619085054386 2201 9 0.017227466849320194 2201 10-11 0.062320823468091646 2201 12-13 0.07761657889000873 2201 14-15 0.3605571596187289 2201 16-17 0.2129615929512596 2201 18-19 0.270179093057223 2201 20-21 0.31074865959007525 2201 22-23 0.19989045065540978 2201 24-25 0.28830760288050783 2201 26-27 -0.0075114762136152535 2201 28-29 0.1712076293481104 2201 30-31 0.22047243668535543 2201 32-33 0.08823044370230093 2201 34-35 -0.01851907386415519 2201 36-37 0.07939484829667975 2201 38-39 0.04634548348290224 2201 40-41 -0.07122127510773879 2201 42-43 0.16352740654355813 2201 44-45 0.16224644617718553 2201 46-47 0.1076687538164336 2201 48-49 0.10788436045872629 2201 50-51 0.10626346146256793 2201 52-53 0.16129050811115064 2201 54-55 0.3330498319285198 2201 56-57 0.22790900140442005 2201 58-59 0.3279910222840243 2201 60-61 0.28045342778421656 2201 62-63 0.3227870904606007 2201 64-65 0.35106673363382157 2201 66-67 0.24554207437721942 2201 68-69 0.10020878719349469 2201 70-71 0.2758102771470874 2201 72-73 0.1301945627664267 2201 74-75 0.06470222766226641 2201 76-77 0.12882779858444593 2201 78-79 0.16255666396381585 2201 80-81 0.15187399508559807 2201 82-83 0.10579629772345811 2201 84-85 0.1678693497106103 2201 86-87 0.17926528555114274 2201 88-89 0.1944483761365312 2201 90-91 0.12475533762761515 2201 92-93 0.16468329577942242 2201 94-95 0.22570079924839703 2201 96-97 0.3391436172654885 2201 98-99 0.2644224400963786 2201 100-101 0.2479149647209553 1101 1 0.18536433323599155 1101 2 0.21355785277347294 1101 3 0.2515390243164539 1101 4 0.4066548440994566 1101 5 0.4265050954773315 1101 6 0.2193343718583094 1101 7 0.6336439588287561 1101 8 0.22078481093566893 1101 9 0.4028453896841242 1101 10-11 0.4268067798173618 1101 12-13 0.48300464046533875 1101 14-15 0.6555234381173634 1101 16-17 0.5630160517442917 1101 18-19 0.5919293237774852 1101 20-21 0.6487899711824738 1101 22-23 0.3490087027759401 1101 24-25 0.5494978936871604 1101 26-27 0.45579395226530295 1101 28-29 0.566032254413912 1101 30-31 0.5521166296930389 1101 32-33 0.3924405103743993 1101 34-35 0.3311899928365776 1101 36-37 0.5547950020367765 1101 38-39 0.5817479113869481 1101 40-41 0.36076247315497056 1101 42-43 0.49689842235147097 1101 44-45 0.522021909655102 1101 46-47 0.4598173982385134 1101 48-49 0.45550416980339037 1101 50-51 0.4825436560825409 1101 52-53 0.5446331932324995 1101 54-55 0.6881937650317624 1101 56-57 0.5642793531344381 1101 58-59 0.7598205624073202 1101 60-61 0.8105200503258629 1101 62-63 0.726928161311001 1101 64-65 0.7371256405373785 1101 66-67 0.6879645105189098 1101 68-69 0.571273189534395 1101 70-71 0.7519763781177602 1101 72-73 0.5575634101342288 1101 74-75 0.5112493529648532 1101 76-77 0.4954197794308115 1101 78-79 0.5741741451813205 1101 80-81 0.5950001555699878 1101 82-83 0.6672604518335845 1101 84-85 0.5883337416993832 1101 86-87 0.6804788796552792 1101 88-89 0.6046743223098829 1101 90-91 0.7416568635751091 1101 92-93 0.6958258787010543 1101 94-95 0.6463194269088355 1101 96-97 0.6958745919292113 1101 98-99 0.6823236345555692 1101 100-101 0.38281424073287695 2304 1 -1.359960196687382E-4 2304 2 -0.14123114491178157 2304 3 -0.02312021399218267 2304 4 0.05746388935359903 2304 5 0.03986767961269777 2304 6 -0.06994061275386798 2304 7 0.240787612236808 2304 8 -0.08702065829146477 2304 9 -0.018027946022080243 2304 10-11 0.07810856392916321 2304 12-13 0.0393670780879134 2304 14-15 0.1857179139479328 2304 16-17 0.03756214076833686 2304 18-19 0.08481225567371808 2304 20-21 0.13775871219960578 2304 22-23 0.041568420675105244 2304 24-25 0.15936930282971673 2304 26-27 -0.0026613589430013462 2304 28-29 0.028993829442399033 2304 30-31 0.10668577855720685 2304 32-33 -0.056022518634343044 2304 34-35 -0.11565507505697781 2304 36-37 0.08361661713332325 2304 38-39 0.03039204096159409 2304 40-41 -0.027431790470920703 2304 42-43 -0.06287591211016519 2304 44-45 0.04955810388965887 2304 46-47 0.056260889686228666 2304 48-49 0.06422925566069182 2304 50-51 0.01844187905750516 2304 52-53 0.07830351595082163 2304 54-55 0.22484311492103615 2304 56-57 0.30934176240997147 2304 58-59 0.2151410272987988 2304 60-61 0.2313415790276352 2304 62-63 0.26331608738255596 2304 64-65 0.14398455946403743 2304 66-67 0.18301626974742646 2304 68-69 0.09270491616381094 2304 70-71 0.2383174439824316 2304 72-73 0.13380286658767915 2304 74-75 0.11043194692613412 2304 76-77 0.24377300346878172 2304 78-79 0.11280032295707443 2304 80-81 0.20601340900607212 2304 82-83 -0.012927096489761425 2304 84-85 0.12390888007037049 2304 86-87 0.18740335111434447 2304 88-89 0.19656715815264292 2304 90-91 0.08758178731281063 2304 92-93 0.08058495473084193 2304 94-95 0.22048645388550625 2304 96-97 0.13778624949058127 2304 98-99 0.12873508628238994 2304 100-101 0.15282348935314616 2202 1 -0.17103664974727195 2202 2 -0.2647175292423114 2202 3 -0.2559875588741676 2202 4 -0.1723885791025026 2202 5 -0.18929235843487646 2202 6 -0.3011418490225566 2202 7 0.15720093377606048 2202 8 -0.3186911036180575 2202 9 -0.2927834436807544 2202 10-11 -0.07747852210489725 2202 12-13 -0.15637097665462107 2202 14-15 -0.09974179709703179 2202 16-17 -0.1550560901969149 2202 18-19 -0.11071562672749735 2202 20-21 -0.11672667651927071 2202 22-23 -0.16430788138003294 2202 24-25 -0.10252972782323866 2202 26-27 -0.18626036455296457 2202 28-29 -0.12349925319448118 2202 30-31 -0.0929540687860424 2202 32-33 -0.12089469664725527 2202 34-35 -0.29869380618241337 2202 36-37 -0.21561451858026004 2202 38-39 -0.1967309634423131 2202 40-41 -0.36659692040851155 2202 42-43 -0.26922489370439706 2202 44-45 -0.18118058664503423 2202 46-47 -0.16683552361310205 2202 48-49 -0.16566374801580253 2202 50-51 -0.19939148009621022 2202 52-53 -0.15580029197220568 2202 54-55 -0.009815137078099667 2202 56-57 -0.0747237591644847 2202 58-59 -0.1855276927821805 2202 60-61 0.02554246047433395 2202 62-63 0.027128186090813244 2202 64-65 -0.055759541134385415 2202 66-67 0.15996061377928328 2202 68-69 0.030048293347370247 2202 70-71 0.06325054698687183 2202 72-73 -0.058424310556226544 2202 74-75 -0.08596466632843658 2202 76-77 -0.0569544649179079 2202 78-79 0.06781552921749068 2202 80-81 0.0445627367599819 2202 82-83 0.09099896171548139 2202 84-85 0.1428814455000449 2202 86-87 0.10032772849613636 2202 88-89 0.15715151500029023 2202 90-91 0.08283389139614883 2202 92-93 0.12288234665033926 2202 94-95 0.16568103127251632 2202 96-97 0.002500645546341218 2202 98-99 0.12078351976270696 2202 100-101 -0.13197335978309255 1102 1 -0.11022416905527166 1102 2 -0.2708664629342046 1102 3 -0.1818202718192623 1102 4 -0.13809377061255645 1102 5 -0.1499865855324245 1102 6 -0.25105423501039326 1102 7 -0.018368430693165294 1102 8 -0.22788981523628848 1102 9 -0.11030776543181986 1102 10-11 -0.10002722891194793 1102 12-13 -0.16191572817013622 1102 14-15 -0.13714778928773796 1102 16-17 -0.12848739422766187 1102 18-19 -0.014663321668678009 1102 20-21 0.006732228457508427 1102 22-23 -0.16955016197783124 1102 24-25 -0.1613744383171678 1102 26-27 -0.23916390810928334 1102 28-29 -0.26384158089114607 1102 30-31 -0.10890830185957157 1102 32-33 -0.18331676828817223 1102 34-35 -0.148573273610765 1102 36-37 -0.06013978230856054 1102 38-39 -0.08870959676500689 1102 40-41 -0.24546269878050708 1102 42-43 -0.14683964020617424 1102 44-45 -0.017160951990398132 1102 46-47 -0.045272854272432994 1102 48-49 0.03276084569320403 1102 50-51 -0.006443395196711776 1102 52-53 -0.02281045114609981 1102 54-55 0.18007911838004986 1102 56-57 0.15427977071797017 1102 58-59 0.16529235917849405 1102 60-61 0.205066863255702 1102 62-63 0.21268529472712316 1102 64-65 0.1980540739818224 1102 66-67 0.11389526790650706 1102 68-69 -0.0369569280425992 1102 70-71 0.1335780106878275 1102 72-73 0.019791073046270924 1102 74-75 0.13003970424338007 1102 76-77 0.1408341675438436 1102 78-79 -0.08083113815819232 1102 80-81 -0.06618264071461155 1102 82-83 -0.02610450891356564 1102 84-85 0.03351573549736031 1102 86-87 0.14780705310858266 1102 88-89 0.134268558204635 1102 90-91 0.08300734688265976 1102 92-93 0.06526992976970547 1102 94-95 0.14117316373679856 1102 96-97 0.03790455627120792 1102 98-99 -0.002723243735172076 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60-61 -0.6684920430281167 1213 62-63 -0.8764432754417726 1213 64-65 -0.8184396454067127 1213 66-67 -0.7010850124965415 1213 68-69 -0.40165844869607525 1213 70-71 -1.3179895369478274 1213 72-73 -0.6951399083643999 1213 74-75 -0.5627688553442702 1213 76-77 -0.5434923601237145 1213 78-79 -0.5582239429101357 1213 80-81 -0.6837859882364334 1213 82-83 -0.799196477586662 1213 84-85 -0.8552651364935961 1213 86-87 -0.8016676921799544 1213 88-89 -0.8243747976539275 1213 90-91 -0.8826669270594145 1213 92-93 -0.7910962535263515 1213 94-95 -0.9309252097972553 1213 96-97 -0.8796939815928226 1213 98-99 -0.7675668496959389 1213 100-101 -0.5994993418668457 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 503.0 3 320.0 4 503.0 5 423.0 6 1175.0 7 9514.0 8 59198.0 9 107906.0 10 96935.0 11 69790.0 12 44894.0 13 31105.0 14 23824.0 15 20050.0 16 18067.0 17 17427.0 18 17653.0 19 18668.0 20 19723.0 21 21136.0 22 22957.0 23 25146.0 24 28117.0 25 33269.0 26 39118.0 27 46429.0 28 54091.0 29 64977.0 30 80135.0 31 103916.0 32 140060.0 33 198870.0 34 299854.0 35 499641.0 36 942262.0 37 1582033.0 38 502136.0 39 1404.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.54019426172405 23.73582178701373 21.605932331312655 15.118051619949563 2 32.16212095622316 25.821594270792335 29.197805464634552 12.818479308349948 3 35.43628050399373 20.736756374127058 27.99344164851163 15.833521473367584 4 29.03849422729834 18.662528529274137 32.66673033031886 19.632246913108663 5 33.86772999741769 17.206354008614827 30.928061296991533 17.99785469697595 6 31.168922433646536 17.472404123856236 34.89901563059756 16.459657811899664 7 35.70175758463224 19.03390406166409 27.242762260718795 18.02157609298488 8 28.833642036052076 29.521918974746825 24.113840578064057 17.530598411137042 9 31.81389284722335 25.695515686935146 24.121314970903455 18.36927649493805 10-11 27.02779998576229 28.731486922587894 27.851535430042752 16.389177661607064 12-13 28.61994054348489 24.244674430863785 27.518237871301377 19.617147154349947 14-15 25.823042233146072 24.57928115447021 28.85233436905321 20.745342243330505 16-17 27.318014698513636 24.891610821719105 29.49407312148808 18.296301358279184 18-19 26.958070669778227 24.979957846579413 29.699150098168374 18.362821385473982 20-21 25.69850679121255 27.06128253465561 28.69615724038914 18.544053433742697 22-23 28.371129655150423 25.170671229036174 27.683156381782354 18.775042734031047 24-25 24.891314957542786 28.242593156863084 27.903316698227677 18.96277518736645 26-27 22.990542898739434 30.188113039959052 27.872638019819252 18.948706041482257 28-29 24.358853173080046 29.08405277359258 27.36609436077232 19.190999692555057 30-31 26.735177794587432 26.48163749223159 27.780441324144476 19.0027433890365 32-33 23.523937178453476 26.317649363616468 31.1653010037362 18.99311245419386 34-35 21.175713470829805 28.567865585658513 29.18445741209814 21.071963531413537 36-37 23.61739441335655 29.6855834122414 27.850123584135915 18.846898590266132 38-39 25.075569171105585 27.0678568799164 27.567778953840033 20.288794995137987 40-41 23.314416264000876 26.34660058791628 27.21930554588784 23.11967760219501 42-43 25.915503976576566 26.188499398375242 28.140037928995365 19.755958696052822 44-45 23.82726209555161 27.715214098967806 28.89096632366045 19.566557481820134 46-47 24.422375678375946 28.374890151081534 27.715476262544104 19.48725790799842 48-49 21.854484286023272 28.52422543124773 29.00114571020359 20.620144572525405 50-51 20.79933821226459 27.150013986482126 30.183736948938584 21.866910852314703 52-53 25.261321725941286 26.49050284725706 27.540906158863987 20.707269267937672 54-55 26.362586565335317 26.407140846600186 27.66461372776684 19.56565886029766 56-57 27.024463218153166 26.176681921553456 27.142360434424802 19.656494425868576 58-59 25.78064164758185 26.437423220905025 27.083715794578534 20.69821933693459 60-61 23.00959755281522 26.5221106968741 26.79730427301405 23.670987477296627 62-63 24.96684375803815 26.474075140384123 27.130231792121286 21.428849309456442 64-65 23.27075351469638 27.416768057423823 28.531287376341748 20.78119105153805 66-67 21.81568960866123 27.61851156710062 27.812145766326267 22.753653057911883 68-69 21.366947781282388 26.776324883834445 31.304577925610072 20.552149409273092 70-71 21.122038014919788 29.556196290460328 29.14492477259248 20.176840922027406 72-73 20.68246816569142 32.64150761260296 26.598334086315127 20.07769013539049 74-75 20.85156733823626 32.88999168316222 26.18923563567605 20.069205342925464 76-77 20.767277508305217 32.65179636955226 26.22624511376872 20.354681008373802 78-79 20.71413058896986 32.30841245134104 26.640009145839045 20.33744781385006 80-81 20.51796399200332 32.22565332449784 26.742911637236123 20.513471046262715 82-83 20.199259503690957 32.15095713656402 26.888673405098295 20.761109954646727 84-85 20.368856840007396 31.78197462678604 26.87676897402423 20.972399559182335 86-87 20.227187652832484 31.645334459024998 27.387714885758392 20.739763002384127 88-89 20.29889262200567 31.6136816924086 27.453546917756483 20.633878767829245 90-91 20.650993105551553 31.113087979624975 27.151956550108437 21.08396236471503 92-93 20.465576801505286 31.373219398271935 26.955600920406482 21.2056028798163 94-95 20.418640344568104 31.334427209433624 26.88330663955776 21.363625806440513 96-97 20.679295472656968 31.47272501715098 26.727260111337543 21.12071939885451 98-99 20.906686867269304 31.152973486517837 26.739190246198667 21.201149400014188 100-101 20.939614627194615 31.205651925420376 26.311146540599218 21.543586906785794 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1720.0 1 1210.0 2 683.5 3 821.0 4 901.5 5 845.0 6 944.0 7 1181.5 8 1440.5 9 1746.5 10 1990.5 11 2143.5 12 2280.0 13 2229.0 14 2110.5 15 2066.5 16 2005.0 17 1922.5 18 1935.5 19 1990.0 20 2166.0 21 2484.5 22 3045.0 23 3866.5 24 5033.0 25 6882.0 26 10153.0 27 15008.5 28 20102.0 29 25122.0 30 30736.5 31 36197.5 32 46774.0 33 66658.5 34 96043.0 35 129246.5 36 168446.5 37 217915.0 38 257032.5 39 267364.5 40 267865.0 41 266731.5 42 266480.0 43 273947.5 44 271892.0 45 261881.5 46 251371.5 47 229988.5 48 202524.5 49 183498.0 50 168394.5 51 156709.5 52 147110.0 53 131790.5 54 113507.5 55 97390.0 56 83537.5 57 72020.0 58 62825.0 59 53930.5 60 44845.0 61 37021.5 62 30392.0 63 24930.5 64 20711.0 65 16658.5 66 13099.0 67 10797.0 68 9320.0 69 7400.5 70 5286.5 71 4030.0 72 3054.5 73 2280.0 74 1838.5 75 1442.0 76 1125.0 77 902.0 78 683.5 79 515.0 80 374.5 81 269.0 82 223.5 83 191.5 84 157.0 85 134.0 86 108.5 87 96.0 88 76.0 89 58.5 90 55.0 91 44.5 92 37.5 93 28.0 94 22.0 95 18.0 96 10.0 97 6.0 98 4.0 99 2.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18444740826692863 2 0.1742628445181395 3 0.14813390755963549 4 0.14073388745751902 5 0.1450442084448343 6 0.12543796961757725 7 0.1011208932510863 8 0.08849508575726904 9 0.08838065245672085 10-11 0.06907956909759234 12-13 0.0879324553629071 14-15 0.09237628186752858 16-17 0.08521466447488751 18-19 0.08145743777355519 20-21 0.07900665792014806 22-23 0.10122579044325548 24-25 0.44713858578368404 26-27 0.28183014703344067 28-29 0.12433178104561139 30-31 0.2309264005062529 32-33 0.2616231333783056 34-35 0.24535453248370423 36-37 0.24768134292818414 38-39 0.26537082397125894 40-41 0.2653517517545009 42-43 0.29185259693978655 44-45 0.26745923170626346 46-47 0.28656959289781164 48-49 0.27002444486021876 50-51 0.2854443321090877 52-53 0.2851582488577173 54-55 0.2603929753974125 56-57 0.27696673176014247 58-59 0.2658857738237258 60-61 0.2427702471129909 62-63 0.23484574105002853 64-65 0.2332436748423538 66-67 0.24313261923139348 68-69 0.24751922908574087 70-71 0.2337586246948207 72-73 0.22515705493694824 74-75 0.23410192459646528 76-77 0.2351890409516731 78-79 0.23716301538612944 80-81 0.2442674161284964 82-83 0.2702247031361781 84-85 0.26426463539929307 86-87 0.2641788104238819 88-89 0.26241463037376395 90-91 0.2535937301231741 92-93 0.23004907853538345 94-95 0.22857098173663595 96-97 0.22409854690687742 98-99 0.24835840662309427 100-101 0.25625430436091956 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5243229.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.58421563780736 #Duplication Level Percentage of deduplicated Percentage of total 1 92.0102581351933 56.66379577888063 2 4.214479785038864 5.190908637660269 3 1.1849832311303936 2.1892878849935964 4 0.6009854223043183 1.4804486336947147 5 0.35414721335832466 1.090493917749382 6 0.2953619708555551 1.0913781182625768 7 0.19829161769651596 0.8548143620374329 8 0.11335210096952175 0.5584560183284433 9 0.11585763580519083 0.6421501464042081 >10 0.7212607669901065 8.852611966373471 >50 0.09623549941017369 4.12887401467418 >100 0.08274728855821215 11.06513039820666 >500 0.010206881517173229 4.245613814669187 >1k 0.0018013280163423249 1.7050693948785969 >5k 0.0 0.0 >10k+ 3.1123155854604647E-5 0.24096691318674732 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT 12572 0.23977590908197985 Illumina Single End PCR Primer 1 (96% over 32bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.002898976947220882 0.0 0.0 2 0.0032422768488654607 0.0 0.0 3 0.0037000100510582315 0.0 1.907221675803212E-5 4 0.00404330995270281 0.0 1.907221675803212E-5 5 0.004634548672201805 0.0 1.907221675803212E-5 6 0.0053783651257650585 0.0 1.907221675803212E-5 7 0.006484553697730921 0.0 1.907221675803212E-5 8 0.007628886703212849 0.0 1.907221675803212E-5 9 0.008506208674082326 0.0 1.907221675803212E-5 10-11 0.012091785424592365 0.0 1.907221675803212E-5 12-13 0.015505712224280115 0.0 3.814443351606424E-5 14-15 0.019568094393740958 0.0 3.814443351606424E-5 16-17 0.023363465528589346 0.0 3.814443351606424E-5 18-19 0.027864508683484928 0.0 5.7216650274096366E-5 20-21 0.03280421282381525 0.0 5.7216650274096366E-5 22-23 0.03796324745686294 0.0 8.582497541114454E-5 24-25 0.04322717928207981 0.0 9.536108379016061E-5 26-27 0.04914910258544877 0.0 9.536108379016061E-5 28-29 0.05535710914018823 0.0 1.1443330054819273E-4 30-31 0.08225847087739253 0.0 1.1443330054819273E-4 32-33 0.09064071014254765 0.0 1.1443330054819273E-4 34-35 0.0983840301463087 0.0 1.1443330054819273E-4 36-37 0.10650879448523037 0.0 1.1443330054819273E-4 38-39 0.11594000567207727 0.0 1.1443330054819273E-4 40-41 0.12620085828789857 0.0 1.1443330054819273E-4 42-43 0.13729135233269424 0.0 1.2396940892720878E-4 44-45 0.14956432381648788 0.0 1.3350551730622484E-4 46-47 0.16276229781304613 0.0 1.430416256852409E-4 48-49 0.17783888516027052 0.0 1.7164995082228908E-4 50-51 0.19307758634993819 0.0 1.7164995082228908E-4 52-53 0.20958458995401497 0.0 1.7164995082228908E-4 54-55 0.22759829868197634 0.0 1.7164995082228908E-4 56-57 0.24727129026788647 0.0 1.7164995082228908E-4 58-59 0.26853681195309226 0.0 1.7164995082228908E-4 60-61 0.29170955531410125 0.0 1.7164995082228908E-4 62-63 0.31644622044926896 0.0 1.7164995082228908E-4 64-65 0.34398650144786735 0.0 1.7164995082228908E-4 66-67 0.37433039830989645 0.0 1.7164995082228908E-4 68-69 0.40639079468014844 0.0 1.7164995082228908E-4 70-71 0.4400532572580751 0.0 1.9072216758032122E-4 72-73 0.47635722185698925 0.0 1.9072216758032122E-4 74-75 0.5156555244869145 0.0 2.0979438433835334E-4 76-77 0.5595883757890414 0.0 2.0979438433835334E-4 78-79 0.6084895395566359 0.0 2.0979438433835334E-4 80-81 0.6626546351494471 0.0 2.0979438433835334E-4 82-83 0.7209584017787511 0.0 2.0979438433835334E-4 84-85 0.7862139914163582 0.0 2.0979438433835334E-4 86-87 0.8569242350467623 0.0 2.0979438433835334E-4 88-89 0.9352252209468631 0.0 2.0979438433835334E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 4700 0.0 77.66989 9 ATCGGGA 8180 0.0 71.21957 2 TCGGGAG 7380 0.0 70.44705 3 CGGGAGA 7210 0.0 69.934265 4 AGAGCGG 5405 0.0 69.032135 8 ATTCGGA 1135 0.0 66.87724 2 GATCGGG 9080 0.0 66.681625 1 CGGAAAG 2720 0.0 65.569626 5 TCGGAGA 1635 0.0 64.69625 4 GTTCGGA 1250 0.0 63.76077 2 ATCCGGA 1180 0.0 61.512543 2 GAGAGCG 7060 0.0 60.308296 7 TCGGGAA 2080 0.0 59.751194 3 AAGCGGC 1475 0.0 59.462 9 AATCGGA 1120 0.0 59.301308 2 GGATCGG 2965 0.0 59.053146 1 TCGGAAA 2140 0.0 57.187088 4 ATCGGAG 1420 0.0 56.455597 3 CGGAGAG 3180 0.0 56.23387 5 GAGGGGC 7220 0.0 55.222824 9 >>END_MODULE